Improve sensitivity for the study of blood diseases by analysis of hemoglobin concentration using a graphene-coated SPR based biosensor

Improve sensitivity for the study of blood diseases by analysis of hemoglobin concentration using a graphene-coated SPR based biosensor | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Improve sensitivity for the study of blood diseases by analysis of hemoglobin concentration using a graphene-coated SPR based biosensor HABIA Mohamed ilyes, Manallah Aissa, Ayadi Khaled This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4574565/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Recent scientific and technical advancements have resulted in the creation of very sensitive biosensing devices for pathogen detection. Surface plasmon resonance (SPR) has developed as an environmentally benign and effective label-free detection method in clinical research, notably for studying biomolecular interactions such as hemoglobin interactions. SPR can be used to assess blood abnormalities such as anemia, which is characterized by a transitory fall in blood hemoglobin concentration due to a decrease in red blood cell count. When compared to gold film sensors, using silver as the metal material and graphene to improves the sensitivity and gives a more distinct reflectance spectrum. The fundamental goal of this research is to improve hemoglobin detection sensitivity by concentration analysis, hence leading to a better knowledge of blood behavior utilizing SPR. This entails examining SPR reflectivity and calculating electromagnetic field dispersion in multilayer structures using a mechanism for wave propagation in stratified media based on transfer matrices. Biophysics SPR optical sensors biosensors blood diseases Hemoglobin (HB) Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4574565","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":313929238,"identity":"d070212d-694b-4071-9e4d-2723bf556e13","order_by":0,"name":"HABIA Mohamed ilyes","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0001-7787-9672","institution":"Ferhat Abbas university of Setif1","correspondingAuthor":true,"prefix":"","firstName":"HABIA","middleName":"Mohamed","lastName":"ilyes","suffix":""},{"id":313929239,"identity":"fc8b6506-d7f9-4749-b5bb-c7064b7d6123","order_by":1,"name":"Manallah Aissa","email":"","orcid":"","institution":"Ferhat Abbas university of Setif1","correspondingAuthor":false,"prefix":"","firstName":"Manallah","middleName":"","lastName":"Aissa","suffix":""},{"id":313929240,"identity":"5bc5179c-c8fd-497d-917a-ade8f8012602","order_by":2,"name":"Ayadi Khaled","email":"","orcid":"","institution":"Ferhat Abbas university of Setif1","correspondingAuthor":false,"prefix":"","firstName":"Ayadi","middleName":"","lastName":"Khaled","suffix":""}],"badges":[],"createdAt":"2024-06-13 08:16:02","currentVersionCode":1,"declarations":{"humanSubjects":true,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":true,"humanSubjectConsent":true,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-4574565/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4574565/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":58329017,"identity":"ca7a9fbb-267b-4e85-93b5-e10a7168b481","added_by":"auto","created_at":"2024-06-14 03:10:21","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":550112,"visible":true,"origin":"","legend":"","description":"","filename":"Articl24.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4574565/v1_covered_014202f9-e266-4557-8703-5c7e9ee154a6.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003eImprove sensitivity for the study of blood diseases by analysis of hemoglobin concentration using a graphene-coated SPR based biosensor\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"SPR, optical sensors, biosensors, blood diseases, Hemoglobin (HB)","lastPublishedDoi":"10.21203/rs.3.rs-4574565/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4574565/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eRecent scientific and technical advancements have resulted in the creation of very sensitive biosensing devices for pathogen detection. 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