Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production

Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production Nazli Ece Varan, Dilek Alagöz, Ali Toprak, Hatice Korkmaz Güvenmez, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4866580/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 29 Oct, 2024 Read the published version in Chemical Papers → Version 1 posted 5 You are reading this latest preprint version Abstract In this study, Fe 3 O 4 -coated multi-walled carbon nanotubes (MWCNT-Fe 3 O 4 ) or nickel oxide-coated multi-walled carbon nanotubes (MWCNT-NiO) were activated with 3-glycidyloxypropyl)trimethoxysilane to create oxirane groups. Pullulanase from Bacillus licheniformis was covalently immobilized on these magnetic MWCNTs to obtain magnetically separable immobilized pullunase preparations (MWCNT-Fe 3 O 4 @Pul or MWCNT-NiO@Pul) for producing maltooligosaccharides (MOS) from pullulan. The highest recovered activity values were obtained as 78% and 85% respectively, for MWCNT-Fe 3 O 4 @Pul and MWCNT-NiO@Pul after 24 h of immobilization at pH 7.0. The optimal pH and temperature were found to be 5.5 and 45°C for the free pullulanase, whereas the corresponding values were 5.5 and 50°C for both immobilized pullulanase preparations. The thermal stability of MWCNT-Fe 3 O 4 @Pul and MWCNT-NiO@Pul increased by 6.2- and 8.2-fold at 50°C. The catalytic efficiencies of MWCNT-Fe 3 O 4 @Pul and MWCNT-NiO@Pul were calculated to be 0.8- and 1.1-fold that of free pullulanase, respectively. After 24 h of hydrolysis, MOS yields were determined to be 470 and 490 mg MOS/g pullulan for MWCNT-Fe 3 O 4 @Pul and MWCNT-NiO@Pul, respectively. The remaining activities were 86% and 85% for MWCNT-Fe 3 O 4 @Pul and MWCNT-NiO@Pul after 10 reuses, respectively. Pullulanase immobilization magnetic multi-walled carbon nanotubes maltooligosaccharide Full Text Cite Share Download PDF Status: Published Journal Publication published 29 Oct, 2024 Read the published version in Chemical Papers → Version 1 posted Reviewers agreed at journal 09 Aug, 2024 Reviewers invited by journal 09 Aug, 2024 Editor invited by journal 08 Aug, 2024 Editor assigned by journal 07 Aug, 2024 First submitted to journal 05 Aug, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4866580","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":338019562,"identity":"d4ac7eb7-4ba6-4971-86a7-0951a8ae5ae6","order_by":0,"name":"Nazli Ece Varan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAtElEQVRIiWNgGAWjYPACGzApQYqWNJgWA6K1HCZBC79E8rMPP3eclzc4wHzwNg/Dn3yCWiRnpBnP7D1z23DDAbZkax4GA8sGQloMzhwwZuBtu51gcIDHTBqohbDL7M8c/8z4t+0cUAv/N+K0GLD3GDPzth0A2cJGnBaJ4z3FzLJtyYYzD7MZW84xMCashb+ZfTPj2zY7eb7jzQ9vvKmQIz5iGBiYwe4kQcMoGAWjYBSMAtwAAB3fMirzdzuEAAAAAElFTkSuQmCC","orcid":"","institution":"Cukurova University: Cukurova Universitesi","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Nazli","middleName":"Ece","lastName":"Varan","suffix":""},{"id":338019563,"identity":"5ca663cb-e069-41c2-9ed5-9b5527e02005","order_by":1,"name":"Dilek Alagöz","email":"","orcid":"","institution":"Cukurova University: Cukurova Universitesi","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Dilek","middleName":"","lastName":"Alagöz","suffix":""},{"id":338019564,"identity":"998edc22-6930-425d-ae3f-74b6b5fe15d3","order_by":2,"name":"Ali Toprak","email":"","orcid":"","institution":"Nevşehir Hacı Bektaş Veli Üniversitesi: Nevsehir Haci Bektas Veli Universitesi","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ali","middleName":"","lastName":"Toprak","suffix":""},{"id":338019565,"identity":"ad4a7625-0da0-420f-95eb-8838a4270c80","order_by":3,"name":"Hatice Korkmaz Güvenmez","email":"","orcid":"","institution":"Cukurova University: Cukurova Universitesi","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Hatice","middleName":"Korkmaz","lastName":"Güvenmez","suffix":""},{"id":338019566,"identity":"4af5058b-d356-4781-8250-ec7e2b3d4a36","order_by":4,"name":"Deniz Yildirim","email":"","orcid":"","institution":"Cukurova University: Cukurova Universitesi","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Deniz","middleName":"","lastName":"Yildirim","suffix":""}],"badges":[],"createdAt":"2024-08-06 07:53:33","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4866580/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4866580/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s11696-024-03764-0","type":"published","date":"2024-10-29T16:20:03+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":68206940,"identity":"61653aff-99e4-40ea-b596-b50e3cde3c07","added_by":"auto","created_at":"2024-11-04 16:33:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1224791,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4866580/v1_covered_f2987f07-fa09-41da-82e3-efa9eee6b358.pdf"}],"financialInterests":"","formattedTitle":"Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"chemical-papers","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"chpa","sideBox":"Learn more about [Chemical Papers](http://link.springer.com/journal/11696)","snPcode":"11696","submissionUrl":"https://www.editorialmanager.com/CHPA/default.aspx","title":"Chemical Papers","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Pullulanase, immobilization, magnetic multi-walled carbon nanotubes, maltooligosaccharide","lastPublishedDoi":"10.21203/rs.3.rs-4866580/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4866580/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn this study, Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e-coated multi-walled carbon nanotubes (MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e) or nickel oxide-coated multi-walled carbon nanotubes (MWCNT-NiO) were activated with 3-glycidyloxypropyl)trimethoxysilane to create oxirane groups. Pullulanase from \u003cem\u003eBacillus licheniformis\u003c/em\u003e was covalently immobilized on these magnetic MWCNTs to obtain magnetically separable immobilized pullunase preparations (MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul or MWCNT-NiO@Pul) for producing maltooligosaccharides (MOS) from pullulan. The highest recovered activity values were obtained as 78% and 85% respectively, for MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul and MWCNT-NiO@Pul after 24 h of immobilization at pH 7.0. The optimal pH and temperature were found to be 5.5 and 45\u0026deg;C for the free pullulanase, whereas the corresponding values were 5.5 and 50\u0026deg;C for both immobilized pullulanase preparations. The thermal stability of MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul and MWCNT-NiO@Pul increased by 6.2- and 8.2-fold at 50\u0026deg;C. The catalytic efficiencies of MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul and MWCNT-NiO@Pul were calculated to be 0.8- and 1.1-fold that of free pullulanase, respectively. After 24 h of hydrolysis, MOS yields were determined to be 470 and 490 mg MOS/g pullulan for MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul and MWCNT-NiO@Pul, respectively. The remaining activities were 86% and 85% for MWCNT-Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e@Pul and MWCNT-NiO@Pul after 10 reuses, respectively.\u003c/p\u003e","manuscriptTitle":"Immobilization of pullulanase from Bacillus licheniformis on magnetic multi-walled carbon nanotubes for maltooligosaccharide production","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-04 02:33:15","doi":"10.21203/rs.3.rs-4866580/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"","date":"2024-08-09T08:33:51+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-09T07:29:43+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"Chemical Papers","date":"2024-08-08T07:22:28+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-07T09:36:13+00:00","index":"","fulltext":""},{"type":"submitted","content":"Chemical Papers","date":"2024-08-06T03:51:32+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"chemical-papers","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"chpa","sideBox":"Learn more about [Chemical Papers](http://link.springer.com/journal/11696)","snPcode":"11696","submissionUrl":"https://www.editorialmanager.com/CHPA/default.aspx","title":"Chemical Papers","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"a329ab7a-6abc-4ecd-b30d-32f0c63c6b8d","owner":[],"postedDate":"September 4th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-11-04T16:25:04+00:00","versionOfRecord":{"articleIdentity":"rs-4866580","link":"https://doi.org/10.1007/s11696-024-03764-0","journal":{"identity":"chemical-papers","isVorOnly":false,"title":"Chemical Papers"},"publishedOn":"2024-10-29 16:20:03","publishedOnDateReadable":"October 29th, 2024"},"versionCreatedAt":"2024-09-04 02:33:15","video":"","vorDoi":"10.1007/s11696-024-03764-0","vorDoiUrl":"https://doi.org/10.1007/s11696-024-03764-0","workflowStages":[]},"version":"v1","identity":"rs-4866580","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4866580","identity":"rs-4866580","version":["v1"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}