Rhizosphere microbiome and plant growth promotion bacteria isolates enhance salinity tolerance in salt-sensitive Brassica fruticulosa

Rhizosphere microbiome and plant growth promotion bacteria isolates enhance salinity tolerance in salt-sensitive Brassica fruticulosa | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 6 March 2025 V1 Latest version Share on Rhizosphere microbiome and plant growth promotion bacteria isolates enhance salinity tolerance in salt-sensitive Brassica fruticulosa Authors : Gloria Escola Oliva 0000-0003-4231-4334 , Anita Bollmann-Giolai , Michael Giolai , Jacob Malone , Levi Yant , Charlotte Poschenrieder , Eliana Bianucci 0000-0002-0697-7870 [email protected] , and Silvia Busoms Authors Info & Affiliations https://doi.org/10.22541/au.174124680.05965302/v1 356 views 181 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Soil salinity is a major stress limiting agricultural productivity worldwide. To counteract the adverse effects of salinity, plants from coastal habitats may engage in positive interaction with microbial partners. We recently discovered inland and coastal populations of Brassica fruticulosa with contrasting salinity tolerance. Here we characterize the microbiomes of salt-tolerant (PST) and salt-sensitive (PSS) populations, analyzing the diversity and abundance of bacteria and fungi taxa in bulk soil, rhizosphere, roots, and leaves of eight populations. We tested in a reciprocal transplant experiment the effects of two salt-tolerant (MST) and two salt-sensitive (MSS) rhizospheric microbiomes on PST and PSS plants under high salinity. Inoculation with MST enhanced salinity tolerance, improved growth, nutritional status, and reduced oxidative stress of PSS plants. Later, we isolated 113 bacterial strains from MST, 26 of which grew in 150mM NaCl and exhibited phosphate solubilization and siderophore production mechanisms. Further screening for nitrogen fixation, auxin production, and ACC deaminase activity allowed us to select 15 top candidates for mediating tolerance. Only five strains significantly promoted salt-sensitive plants growth under saline conditions. These findings highlight that the microbiome of species adapted to harsh conditions harbor a reservoir of potentially beneficial microorganisms that may improve plant resilience to salinity. Supplementary Material File (escola_bf_microbiome_.docx) Download 304.17 KB Information & Authors Information Version history V1 Version 1 06 March 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords brassica fruticulosa genome growth microbiome plant growth-promoting bacteria (pgpb) soil salinity Authors Affiliations Gloria Escola Oliva 0000-0003-4231-4334 Universitat Autonoma de Barcelona Departament de Biologia Animal de Biologia Vegetal i d'Ecologia View all articles by this author Anita Bollmann-Giolai John Innes Centre View all articles by this author Michael Giolai John Innes Centre View all articles by this author Jacob Malone John Innes Centre View all articles by this author Levi Yant University of Nottingham School of Life Sciences View all articles by this author Charlotte Poschenrieder Universitat Autonoma de Barcelona Departament de Biologia Animal de Biologia Vegetal i d'Ecologia View all articles by this author Eliana Bianucci 0000-0002-0697-7870 [email protected] Universitat Autonoma de Barcelona Departament de Biologia Animal de Biologia Vegetal i d'Ecologia View all articles by this author Silvia Busoms Universitat Autonoma de Barcelona Departament de Biologia Animal de Biologia Vegetal i d'Ecologia View all articles by this author Metrics & Citations Metrics Article Usage 356 views 181 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Gloria Escola Oliva, Anita Bollmann-Giolai, Michael Giolai, et al. Rhizosphere microbiome and plant growth promotion bacteria isolates enhance salinity tolerance in salt-sensitive Brassica fruticulosa. Authorea . 06 March 2025. DOI: https://doi.org/10.22541/au.174124680.05965302/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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