Fungal-Mediated Green Synthesis of ZnO–MnO Nanocomposites with Antimicrobial and Anticancer Properties

Fungal-Mediated Green Synthesis of ZnO–MnO Nanocomposites with Antimicrobial and Anticancer Properties | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fungal-Mediated Green Synthesis of ZnO–MnO Nanocomposites with Antimicrobial and Anticancer Properties Ahmed Alhujaily, Samy Selim, Amer M. Abdelaziz, Ebrahim Said, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8631439/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The urgent need for sustainable therapeutic nanomaterials has driven interest in green synthesis routes. In this study, zinc oxide–manganese oxide nanocomposites (ZnO–MnO NCs) were mycosynthesized using the extracellular filtrate of Aspergillus terreus . Characterization by UV–visible spectroscopy, FTIR, XRD, TEM, and DLS confirmed their crystalline structure, functional groups, and nanoscale morphology. The NCs showed a distinct absorption at ~ 320 nm with average particle sizes of 75 nm (TEM) and 99 nm (DLS). Antibacterial assays revealed strong activity against Escherichia coli and Bacillus subtilis , with inhibition zones surpassing ceftriaxone and MIC values of 64–256 µg/mL. Time-kill analysis demonstrated rapid bacterial reduction at higher doses. Cytotoxicity studies indicated good safety in normal cells (IC₅₀ = 360.7 µg/mL) and potent anticancer activity against MCF-7 cells (IC₅₀ = 106.25 µg/mL). These findings highlight fungal-mediated biosynthesis as an eco-friendly route for producing ZnO–MnO NCs with antimicrobial and anticancer potential. Nanoscience Mycosynthesis Aspergillus terreus ZnO–MnO nanocomposites Antibacterial activity Anticancer potential Eco-friendly synthesis Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Introduction The rapid spread of developed multidrug-resistant (MDR) microorganisms represent a critical global health threat, significantly undermining the effectiveness of conventional antimicrobial therapies. The MDR pathogenic bacteria including Staphylococcus aureus ( S. aureus ), Klebsiella pneumoniae ( K. pneumoniae ) and E. coli have gotten resistance to multiple antimicrobial classes with increased difficulty of infections treatment and consequent lengthy diseases, elevated cost of healthcare, as well as increased mortality rates ( 1 ). Conventional antimicrobials are often insufficient due to the MDR pathogens can form biofilms and express efflux pumps, which protect them from both antibiotics and host immune responses, thereby complicating treatment outcomes ( 2 ). Researchers are actively seeking alternative antimicrobial agents that offer high effectiveness while being more cost-efficient, aiming to overcome limitations of current treatments. Nanotechnology has emerged as a hopeful substitute for conventional antimicrobials in combating MDR bacteria, offering innovative strategies through use of nanoparticles (NPs) with potent antimicrobial properties. Metal oxide NPs generate reactive oxygen species (ROS), disrupt bacterial membranes integrity, and interfere with DNA replication and protein synthesis with consequent exhibition of broad-spectrum antibacterial effects ( 3 , 4 ). Unlike traditional antimicrobials, NPs can concurrently target multiple bacterial components which reduce the development of prospected resistance. Mycosynthesis of NPs represent cost-effective and ecological approach that utilizes fungi to synthesize metallic NPs, as it avoids toxic chemicals and operates under mild conditions by unique biochemical pathways ( 5 ). These pathways typically involve the metal salts interaction with fungal biomass or extracellular filtrates, with consequent stabilized metal oxide NPs formation through precipitation or thermal decomposition. The mycosynthesized NPs often exhibit enhanced bioactivities including anticancer, antioxidant, and antimicrobial properties, making them attractive for biomedical and agricultural applications ( 6 ). Zinc oxide NPs eco-friendly method, had superior purity, hexagonal shape (16–41 nm), and higher antimicrobial, antidiabetic (84.37% α-amylase inhibition), and anticancer activity (notably against MCF-7 cells) due to active phytochemicals like alkaloids ( 7 ). Manganese oxide (MnO) NPs are biocompatible, with minimal hemolysis and notable antibacterial activity. These NPs have potential inhibitory activity, with about 666.44 µg/mL inhibitory concentration fifty (IC₅₀), against the colorectal HT29 cancer cells and promote the apoptotic process via downregulation Bcl-2 and Bcl-xL anti-apoptotic proteins ( 8 ). Additionally, quick targeted antimicrobial effects towards phytopathogenic bacteria more than fungi by the mycosynthesized MnO NPs was demonstrated ( 9 ). Thus, this study aimed for biosynthesis and characterization of ZnO-MnO NCs employing A. terreus . Also, for evaluation of their antimicrobial efficiency towards numerous bacterial species as well as exploration of their possible anticancer activity. Materials and Methods Growth media, chemicals, and reagents Czapek Dox broth (CDB) and Czapek Dox Agar (CDA) media were purchased from Himedia, Cairo, Egypt. Sodium hydroxide (NaOH) precipitating agent, zinc nitrate hexahydrate Zn(NO 3 ) 2 .6H 2 O and manganese acetate tetra hydrate (Mn (CH 3 COO) 2 .4H 2 O) were obtained from Sigma-Aldrich, Germany. Mycosynthesis of ZnO-MnO NCs Aspergillus terreus (OR125572) that was isolated in a previous study ( 10 ) was used for biosynthesis of ZnO-MnO NCs. Three disks of A. terreus , each with a diameter of 0.8 cm and cultured for 72 hrs, were used to inoculate CDB fungal medium (100 mL, pH = 7) followed by shaking incubation 6 for days at 150 rpm and 30 ± 2°C. The A. terreus biomass was subsequently collected by filtration and rinsed 3 times using distilled water for complete removing of all residual medium constituents. The harvested biomass (10 gm) suspended in distilled water (50 mL) with further shaking incubation (150 rpm) for 48 hrs followed by centrifugation at 7000 rpm to obtain the A. terreus fungal biomass filtrate (FBF) that served as the biocatalyst for ZnO-MnO NCs synthesis. For this purpose, 3.0 mM of Zn(NO 3 ) 2 .6H 2 O and 2.0 mM of Mn(CH 3 COO) 2 .4H 2 O were added to the FBF (pH = 8) with dark shaking incubation (150 rpm) for 24 hrs at 28 ± 2°C. The oven was then used for NCs drying for 24 hrs at 120°C ( 11 , 12 ). Characterization of ZnO-MnO NCs Formation of ZnO-MnO NCs was initially indicated by the change in FBF color of from yellow to light brown after metal precursors bio-reduction. The UV-visible spectrophotometer (JENWAY 6305, Staffordshire, UK) was then utilized to record the absorbance at wavelengths range of 200–800 nm. Additionally, the FTIR spectroscopy (Cary-660 model, KBr Pellet technique) was employed for identification of the functional groups on the biosynthesized ZnO-MnO NCs. The TEM was used to analyze particle size and morphology (TEM, JEM-2100 Plus, Jeol, Japan), while DLS provided insights into particle size distribution (Nano ZS, Malvern, UK). Also, the XRD pattern was used for determination of crystalline nature and size (XRD-6000, Shimadzu Scientific Instruments, Japan), while the scanning electron microscopy (SEM) imaging (SEM, ZEISS, EVO-MA10, Germany) revealed surface morphology. Finally, the ZnO-MnO formulation elements and their distribution were examined with spectroscopy based on the energy-dispersive X-ray (EDX) analysis (EDX, Bruker, Germany). Antimicrobial activity of ZnO-MnO NCs Well diffusion assay The inhibition zone diameter (IZD), reflecting the biosynthesized ZnO-MnO NCs antimicrobial activity, was measured against S. aureus ATCC® 25923, B. subtilis ATCC® 6633, E. coli ATCC® 25922, and K. pneumoniae ATCC® 700603 following the well diffusion assay conducted under restricted aseptic conditions. In brief, prepared suspension of each bacterial isolate of 0.5 McFarland standard was transferred by cotton swab to Muller Hinton agar (MHA) in a way consensus with lawn growth formation. Sterile borer was then used to make 6 wells on the surface of MHA and 100 µL of the mycosynthesized ZnO-MnO NCs (1000 µg/mL), ZnSO 4 (1000 µg/mL), MnSO 4 (1000 µg/mL), fungal filtrate, dimethyl sulfoxide (DMSO) (10% V/V) as negative control, or ceftriaxone (50 µg/mL) standard positive control (PC), all dissolved in DMSO was aseptically added to each well. The plates were shortly incubated for 30 min at 37°C followed by incubation for 24 hrs at 37°C and measurement of IZD formed around the wells ( 13 ). Accordingly, the ZnO-MnO NCs percent inhibition (PI) was calculated using Eq. (1) for the tested strains ( 14 ). \(\:\text{P}\text{e}\text{r}\text{c}\text{e}\text{n}\text{t}\:\text{I}\text{n}\text{h}\text{i}\text{b}\text{i}\text{t}\text{i}\text{o}\text{n}\:\left(\text{P}\text{I}\right)=\:\:\frac{\text{I}\text{Z}\text{D}\:\text{c}\text{a}\text{u}\text{s}\text{e}\text{d}\:\text{b}\text{y}\:\text{t}\text{h}\text{e}\:\text{Z}\text{n}\text{O}\:\text{N}\text{P}\text{s}}{\text{I}\text{Z}\text{D}\:\text{c}\text{a}\text{u}\text{s}\text{e}\text{d}\:\text{b}\text{y}\:\text{v}\text{a}\text{n}\text{c}\text{o}\text{m}\text{y}\text{c}\text{i}\text{n}}\:\:\text{X}\:\:\:100\) (Eq. 1) Quantitative dilution method The mycosynthesized ZnO-MnO NCs ability for bacterial strains growth suppression was assessed employing broth microdilution method via calculation of the MIC of the NCs as well as its minimum bactericidal concentration (MBC) ( 15 ). Briefly, ZnO-MnO NCs suspension was serially diluted in Muller Hinton broth (MHB), aseptically in the 96-well microtiter plate raw, with concentration ranged from 512 µg/mL to 16 µg/mL. The 0.5 McFarland standard of the examined bacterial strain (10 µL) inoculated into each well in the raw followed by aerobic incubation for 24 hrs at 37°C. Resazurin indicator was the and incubated for 2 hrs at 37°C. The minimum dose of mycosynthesized ZnO-MnO NCs showing no resazurin blur color change verified as the MIC of the NCs. Samples from wells showing blue color were subsequently transferred to plate of sterile MHA and incubated for 24 hrs at 37°C. The minimum ZnO-MnO NCs concentration showing inhibition of bacterial growth verified as the MBC of the NCs. ( 13 ). The MIC index or tolerance level of bacterial strain to ZnO-MnO NCs determined following Eq. (2). \(\:\text{T}\text{o}\text{l}\text{e}\text{r}\text{a}\text{n}\text{c}\text{e}\:\text{l}\text{e}\text{v}\text{e}\text{l}\:=\:\:\frac{\text{M}\text{B}\text{C}}{\text{M}\text{I}\text{C}}\) (Eq. 2) Consequently, the results recorded as ZnO-MnO-BNPs bacteriostatic or bactericidal activity at tolerance value ˃ 4 or ≤ 4, respectively ( 16 ). Time-kill kinetics Each microbial cultures (OD600 > 0.800) was diluted to about 10 6 colony forming unit (CFU)/mL and treated, in triplicate, with concentrations equivalent to 0.5 MIC, MIC, or 2 MIC of the tested ZnO-MnO NCs, along with ceftriaxone at 50 µg/mL as a PC in tryptic soy broth. During 24 hrs culture incubation at 37°C, aliquots were taken from each treatment at various time intervals and serially diluted in phosphate-buffered saline. The diluted samples inoculated onto tryptic soy agar followed by incubation for 20 hrs at 37°C and calculation of the viable CFU/mL ( 17 ) . Cytotoxicity The cytotoxicity of different biosynthesized ZnO-MnO NCs concentration ranged from 1000 µg/mL to 31.25 µg/mL towards the normal Wi-38 and cancerous MCF-7 cell lines was evaluated following MTT procedures ( 18 ). The tested cell line was transferred and incubated in 96-well tissue culture plate, the growth medium was decanted, and the formed sheet of monolayer cells was then washed. Afterward, the cells was treated with the mycosynthesized ZnO-MnO NCs serially diluted using RPMI growth medium, untreated cells was used as a control. Following suitable plates incubation, the growth medium was discarded, the gotten formazan crystals dissolved in DMSO, and Finally, the optical density at 560 nm (OD 560 ) was determined while the background subtracted at 620 nm for cell amount determination. Finally, the viability and inhibition percent for the tested cell were calculated following Equations (3) and (4), respectively. $$\:\text{V}\text{i}\text{a}\text{b}\text{i}\text{l}\text{i}\text{t}\text{y}\:\text{%}=\:\frac{\text{T}\text{r}\text{e}\text{a}\text{t}\text{m}\text{e}\text{n}\text{t}\:\text{O}\text{D}}{\text{C}\text{o}\text{n}\text{t}\text{r}\text{o}\text{l}\:\text{O}\text{D}}\:\text{X}\:100\:\:\:\:\:\:\:\:\:\:\:\left(\text{E}\text{q}\text{u}\text{a}\text{t}\text{i}\text{o}\text{n}\:3\right)$$ $$\:\text{I}\text{n}\text{h}\text{i}\text{b}\text{i}\text{t}\text{i}\text{o}\text{n}\:\text{%}=100-\text{V}\text{i}\text{a}\text{b}\text{i}\text{l}\text{i}\text{t}\text{y}\:\text{%}\:\:\:\:\:\:\:\:\:\:\left(\text{E}\text{q}\text{u}\text{a}\text{t}\text{i}\text{o}\text{n}\:4\right)$$ Statistical analysis The primary data was calculated as the mean (M), median, standard error (SE), standard deviation (SD), and relative standard deviation (RSD) employing GraphPad Prism software (v8). Also, it was employed for figures construction and M ± SD presentation of the findings of the triplicated experiments. As well, the frequencies and percentages, as the calculated descriptive statistics, were applied for investigation of our results. Results and Discussion Mycosynthesis of ZnO-MnO NCs UV-visible spectroscopy The ZnO-MnO NCs were effectively biosynthesized utilizing the cell-free extracellular filtrate obtained from A. terreus culture of 6 days old. The biosynthesized NCs optical properties confirmed their nanoscale nature, as evident from a redshift in the absorption bands and characteristic peak observed at 320 nm (Fig. 1 ), as recorded through UV–vis spectroscopy. The biological synthesis approach for ZnO-MnO NCs has proven to be promising because of its eco-friendliness, cost-effective, rapid, as well as enhanced yield ( 19 ). Typically, pure ZnO NPs exhibit a strong absorption band around 365–370 nm, corresponding to ZnO intrinsic band gap transition. In our study, the observed blue shift to 320 nm could attributed to NCs reduced particle size, which leads to quantum confinement effects, as well as the interaction between ZnO and MnO phases within the nanocomposite. Such a shift in the absorption edge has been reported for other bimetallic oxide nanostructures which confirm the successful ZnO–MnO NCs development and optical properties modification. Also, El-Moslamy et al. ( 9 ) recorded an absorbance intensity with a peak at 350 nm for MnO NPs. Zhang et al. ( 20 ) recorded the appearance of characteristic band of 324 nm for AGE-Mn NPs, and Ameen ( 11 ) detected a peak at 560 nm in Ag-Cu BNPs. Gaber et al. ( 21 ) found a peak at 375 nm for ZnO-CuO BNPs. Previously, white-rot fungi, including Phanerochaete chrysosporium , utilized in ZnO NPs biosynthesis ( 22 ). As well, A. terreus fungal strain likely secretes various extracellular enzymes, proteins, and other biologically active substances which facilitate NCs biosynthesis ( 23 ). For instance, Kaur et al. ( 24 ) revealed ZnO NPs biosynthesis using the endophytic fungus Trichoderma viride . Similarly, Periconium sp., another endophytic fungus, was utilized by Ganesan et al. ( 25 ) for the ZnO NPs sol-gel biosynthesis. Zhang et al. ( 20 ) recorded MnO NPs mycosynthesis utilizing Arcopilus globulus endophytic fungus. In addition, Abdelrahman et al. ( 26 ) successfully fabricated Ag-Au NCs through mycosynthesis involving A. fumigatus and A. flavus . On a similar note, Sayed et al. ( 27 ) described the eco-friendly production of Ag-Cu BNPs mediated by S. aureus . These BNPs show significant potential in various applications, particularly in plant disease management. Analysis based on FTIR The FTIR of biosynthesized ZnO-MnO NCs using A. terreus fungal extract illustrated in Fig. (2A). Functional groups present in the fungal metabolites, including amine (-NH 2 ), carbonyl (C = O), and hydroxyl (-OH), serve for metal ions active binding and facilitating nucleation and NCs formation ( 28 ). The FTIR analysis revealed characteristic peaks within wavelength ranged from 400 cm − 1 to 4000 cm − 1 , including 3718, 2351, 2236, 2150, 2031, 1910, 1568, 1400, 1027, 923, 775, 621, and 456 cm − 1 . The characteristic peak of 3718 cm − 1 corresponding to hydroxyl (O–H) groups existence ( 29 ). Peaks within the 2351–2031 cm − 1 range indicates the stretching of O = C = O and C = C, highlighting the carboxylic and alkene groups occurrence ( 30 ). These peaks also signify asymmetrical and symmetrical C–H stretches representing the presence of methyl groups. The specific peak at 1568 cm − 1 representing C–N stretching in amines, while the characteristic peak near 1400 cm − 1 suggests aromatic amines existence ( 31 ). Additionally, the typical peak at 1027 cm − 1 correlated to the C–O ( 32 ). Furthermore, the peaks present within 500–1000 cm − 1 range are indicative of metal-oxygen bond formation, confirming the successful synthesis of ZnO-MnO NCs ( 33 ). Assessment based on XRD The XRD pattern of the mycosynthesized ZnO–MnO NCs is shown in Fig. (2B). The identified diffraction peaks confirm the coexistence of both ZnO and MnO phases. For ZnO, sharp peaks showed with 2θ of 31.8°, 34.6°, 36.4°, 47.7°, 56.7°, 62.9°, 66.5°, 68.0°, 72.5°, and 77.0° were respectively indexed to the planes (100), (002), (101), (102), (110), (103), (200), (112), (004), and (202) representing the hexagonal wurtzite structure, the findings in accordance with the reference JCPDS card no. 36-1451 and previous results reported by Kamal et al. ( 31 ) and Motelica et al ( 35 ). Similarly, Sharma et al. ( 22 ) identified hexagonal-phase ZnO with reflections at (100), (002), (101), (102), (110), (200), (112), (201), (004), and (202) planes. Modi et al. ( 36 ) similarly observed comparable peaks for the ZnO-NPs produced by the extracts of onion peel waste, which line up with Bragg's reflections indexed to the planes (002), (101), (102), (110), (103), and (201). Meanwhile, specific peaks observed at 2θ = 28.8°, 37.5°, 43.4°, and 56.5° were assigned to the (200), (220), (311), and (400) cubic MnO planes (rock-salt phase), which is consistent with the standard JCPDS card no. 07-0230 ( 37 ). Detection of well-defined and sharp peaks reflects the ZnO–MnO NCs high crystallinity. Furthermore, the average crystallite size of the mycosynthesized ZnO–MnO NCs was approximately 77 nm as determined following Debye–Scherrer equation, D = Kλ/βcosθ with K = 0.9 and Cu-Kα radiation (λ = 0.15406 nm) ( 39 ). Examinations based on TEM and DLS The TEM image illustrates the structural characteristics of ZnO-MnO NCs synthesized via fungal-mediated processes using A. terreus extract (Fig. 3 A). The NPs exhibit a layered, sheet-like morphology with overlapping, thin crystalline plates, indicative of well-formed bimetallic oxide structures. This unique morphology not only facilitate the formation of these thin layers but also influence the structural arrangement and uniformity of the NPs ( 40 ). The presence of multiple, nearly transparent layers suggests that the particles are nanometer-sized, the size was ranged of 50–100 nm where the average size was 75 nm. Lopez-Ubaldo et al. ( 41 ) reported that biosynthesized Ag-Cu BNPs using Ricinus communis exhibited particle sizes of less than 100 nm. Similarly, Sayed et al. ( 27 ) demonstrated that synthesized Ag, Cu, and their bimetallic NCs were within the nanoscale, with spherical crystals measuring 20 nm, 40 nm, and 80 nm, respectively. Abdelrahman et al. ( 26 ) described Ag-Au BNPs of 10–25 nm size, a sphere-like polyhedron morphology and a predominant truncated tetrahedron topographical pattern. The particle size determined by DLS (Fig. 3 B) was larger than that observed in TEM images. This discrepancy arises because the DLS evaluate the NCs hydrodynamic size in solution, that accounts for solvent molecules surrounding the NCs, particle aggregation, overlapping, as well as the electrical double layer creation. In contrast, TEM imaging provides a direct visualization of individual NPs, allowing the exclusion of aggregates from the size analysis ( 42 ). Our mycosynthesized ZnO-MnO NCs characterized by size range of 80–110 nm, average size about 99 nm, as well as 0.321 polydispersity index (PDI) which indicate a homogenous colloidal solution. Similarly, Elkhodary et al. ( 43 ) reported size distribution about 66.52, 77.45, and 76.85 nm for actinomycete filtrate-based biosynthesized B 2 O 3 NPs, ZnO NPs, and ZnO-B 2 O 3 BNPs, respectively, based on DLS measurements. Investigation using SEM and EDX The SEM image (Fig. 4 A) illustrates the surface morphology of the biosynthesized ZnO–MnO nanocomposite. The material displays irregular, layered structures with interconnected surfaces, suggesting high crystallinity and particle aggregation. This morphology may enhance surface area and reactivity, making the composite potentially applicable in environmental and biomedical fields ( 44 ). Elkhodary et al. ( 43 ) also observed spherical aggregates and foam-like NPs clustering in biosynthesized ZnO–B 2 O 3 composites using fungal filtrate. The EDX spectrum (Fig. 4 B) displays the characteristic peaks of Zn, Mn, O, and C, confirming the successful formation of ZnO–MnO nanocomposites. Quantitative analysis revealed the presence of 17.8 at% Zn (45.9 wt%), 4.1 at% Mn (8.9 wt%), and 51.4 at% O (32.4 wt%). A minor contribution of carbon (26.7 at%, 12.6 wt%) was also detected, which is likely attributed to residual organic metabolites from the fungal filtrate or the carbon substrate used during SEM sample preparation. The relative abundance of Zn and O is consistent with the presence of ZnO, while the detection of Mn and O supports the coexistence of MnO phases within the nanocomposites. These findings verify the successful biosynthesis of ZnO–MnO nanocomposites with an integrated elemental composition. EL-Moslamy et al. ( 45 ) reported a similar biosynthetic approach for ZnO-MnO NCs, resulting in lateral flake-like structures with varying particle sizes. Elemental analysis in their study showed Zn (60.03%), Mn (2.091%), and O (37.06%), reflecting variability introduced by fungal synthesis conditions. Previous literature also describes diverse morphologies such as cubic ZnO, spherical MnO 2 , and sheet-like or nanorod structures, influenced by precursor type, fungal metabolites, and synthesis methods ( 46 , 47 ). Antimicrobial activity of ZnO-MnO NCs Well diffusion assay The diffusion method demonstrated the ZnO-MnO NCs (1000 µg/mL) inhibitory effect against a panel of bacterial isolates, as evidenced by the formation of inhibition zones as illustrated in Figs. 5 & 6 . Specifically, B. subtilis showed the largest IZD (36.66 ± 0.58 mm), followed by E. coli (35 ± 0 mm), S. aureus (29 ± 1 mm), and K. pneumoniae (12.33 ± 0.58 mm). When compared to ceftriaxone (50 µg/mL), the ZnO-MnO NCs showed comparable or even superior activity against E. coli and B. subtilis , as indicated by the PI values of 108.25% and 105.77%, respectively. However, the NCs were less effective, PI = 72.55%, against K. pneumoniae (Table 1 ). In comparison to ceftriaxone, the higher action of the NC against E. coli and B. subtilis indicate strong inhibitory mechanism that may involve the destruction of bacterial cell membranes and intracellular functions. This is in line with new research showing how metal NPs have antibacterial properties, which frequently include producing ROS and interfering with bacterial enzyme systems ( 48 ). The inherent resistance mechanisms, like efflux pumps, may be the cause of the decreased effectiveness against K. pneumoniae since they can hinder the uptake and action of NPs ( 49 ). The significant inhibition observed against S. aureus , a clinically relevant pathogen, is particularly noteworthy, as it highlights the potential of ZnO-MnO NCs in combating antimicrobial-resistant strains ( 50 ). Broth microdilution assay The MIC of the mycosynthesized ZnO-MnO NCs obtained against a panel of bacterial isolates was in the range of 64–256 µg/mL (Table 2 ). Additionally, the MBC were higher than the MIC, with values of 256 µg/mL for S. aureus , 128 µg/mL for B. subtilis , 256 µg/mL for E. coli , and 512 µg/mL for K. pneumoniae . Additionally, the findings illustrated a calculated MIC index (MBC/MIC ratio) in the range of 2–4 indicating a bactericidal effect for all tested isolates (Fig. 6 ). The MIC index of 4 or below is typically indicative of killing rather than mere inhibition ( 51 ). The observed variability in MIC and MBC values across different bacterial species could be associated with the differences of bacterial wall components and physiological responses to the NCs. The tested B. subtilis and S. aureus , Gram-positive bacteria, exhibited lower MICs, potentially resulted from the less complexed bacterial wall, which may facilitate the NCs penetration and consequently enhance their intracellular targets interaction. Conversely, K. pneumoniae and E. coli , Gram-negative bacteria complexed with outer bacterial cell membrane, may pose greater barrier to NPs uptake, resulting in higher MICs ( 52 ). The observed efficacy of ZnO-MnO NCs against clinically significant pathogens like S. aureus and K. pneumoniae highlights their potential as novel antimicrobial agents, particularly in the face of increasing antimicrobial resistance. Further investigation into the specific mechanisms of action is warranted to fully realize their therapeutic potential. Time-kill assay of ZnO-MnO NCs The mycosynthesized ZnO-MnO NCs' antibacterial activity against S. aureus was demonstrated by the time-kill experiment, which showed a concentration-dependent decrease in viable bacterial cells over a 24 hrs period. Throughout the experiment, the PC, which was devoid of ZnO-MnO NCs, demonstrated steady bacterial growth and maintained a log CFU of roughly 6. A significant and quick drop in log CFU was seen at treatment with 2 MIC, reaching about 3.5 within 24 hrs, suggesting a strong bactericidal action. The MIC also resulted in a significant reduction in bacterial viability, albeit at a slower rate, reaching a log CFU of approximately 3.5 by 24 hrs. In contrast, the ½ MIC treatment exhibited a less pronounced reduction in log CFU, suggesting a diminished bactericidal effect at this lower concentration (Fig. 7 ). Recent research showing prospective metal nanomaterials with strong bacterial inhibitory activity is consistent with the observed time-dependent and concentration-dependent bactericidal activity of ZnO-MnO NCs against S. aureus . The quick death seen at 2 MIC points to a strong and direct contact between the BNPs and the bacterial cells, possibly including oxidative stress and membrane rupture. This is consistent with findings that demonstrate the ability of metal NPs to induce ROS production with consequent microbial cell membranes disruption and death ( 54 ) The sustained bactericidal effect observed at MIC, although slower, underscores the potential of ZnO-MnO NCs for sustained antimicrobial activity. The diminished effect at 1/2 MIC highlights the importance of concentration in achieving optimal bactericidal efficacy. The observed efficacy against S. aureus , a clinically relevant pathogen, is particularly significant in the context of increasing antimicrobial resistance. Several research discovered metal NPs applications to alternative the conventional antimicrobials, particularly against MDR strains ( 55 ). The TKA performed for ZnO-MnO NCs against B. subtilis ATCC 6633 demonstrated dose-dependent bactericidal effect. At ½ MIC, the NCs induced a moderate reduction in bacterial viability, decreasing to 4.5 log CFU/mL over 24 hrs. At the MIC, a more pronounced reduction was observed, with bacterial counts dropping to 3.5 log CFU/mL. The most significant antimicrobial activity was seen at 2 MIC, where the BNPs achieved near-complete eradication (2 log reduction) within 4–6 hrs, sustaining suppression up to 24 hrs. In Fig. 8 The 2 MIC concentration’s rapid bactericidal effect (12 hrs) suggests that ZnO-MnO NCs disrupt critical bacterial functions, possibly through: interference with metabolic enzymes (Mg²⁺-dependent pathways in B. subtilis ) ( 56 ) or DNA damage due to Zn²⁺ ion release ( 57 ). The TKA demonstrated that ZnO-MnO NCs exhibit concentration-dependent bactericidal activity against E. coli . At ½ MIC, a moderate reduction in bacterial viability was observed, with log CFU decreasing from 6.5 to ~ 4.5 over 24 hrs. The MIC induced a more significant decline (~ 3.5 log CFU), while 2 MIC achieved near-complete eradication (~ 3 log CFU) within 6–8 hrs, sustaining suppression up to 24 hrs (Fig. 9 ). This efficacy in accordance with current studies highlighting the metal NPs microbial inhibitory potential, particularly towards Gram-negative bacteria like E. coli , often attributed to membrane disruption and intracellular oxidative stress ( 55 ). The observed activity against E. coli , a common cause of both community-acquired and nosocomial infections, underscores the potential clinical relevance of ZnO-MnO NCs. The diminished bactericidal effect at ½ MIC emphasizes the critical role of concentration in achieving optimal antimicrobial activity. Given the increased E. coli frequency with resistance phenotype, these findings support the exploration of ZnO-MnO NCs as a potential alternative or adjunct therapy. The TKA revealed a concentration-dependent bactericidal effect of ZnO-MnO NCs against K. pneumoniae over a 24 hrs period. The PC, devoid of BNPs, exhibited stable bacterial counts, confirming viable growth under the experimental conditions. At exposure to 2 MIC, a rapid and substantial reduction in viable K. pneumoniae was observed, culminating in a significant decrease in log CFU by 24 hrs, indicating the potent bactericidal activity (Fig. 10 ). Current reports discovered possible ZnO-MnO NCs application to alternatives the conventional antimicrobials, particularly against MDR K. pneumonia ( 58 ) Cytotoxicity Before evaluating a new compound for its potential anticancer activity, it's crucial to assess its toxicity towards normal cell lines. This initial step helps determine the compound's general cytotoxicity and selectivity. If a compound exhibits significant toxicity against normal cells, it may not be a suitable candidate for further development as an anticancer agent, as it could cause unacceptable side effects in patients ( 59 ). Early evaluating toxicity using normal cell lines at antimicrobial development, could prioritize compounds that demonstrate a more favorable therapeutic window, meaning that the drug less toxic healthy cells than to cancer cells. Our mycosynthesized ZnO-MnO NCs cytotoxicity toward Wi-38 normal cell line shown in Fig. (11A). Several ZnO-MnO NCs doses were evaluated and the results revealed IC₅₀ about 360.7 µg/mL, which confirms its biosafety or non-toxicity (IC₅₀ ≥ 90 µg/mL) ( 60 ). On the other hand, the mycosynthesized ZnO-MnO NCs malignancy inhibitory ability in case of MCF-7 cell line presented in Fig. (11B) with an IC₅₀ about 106.25 µg/mL. Several earlier reports indicate the BNPs promising antitumor activities towards malignant cells ( 61 , 62 ). Zamani-Roudbaraki, Aghajanzadeh-Kiyaseh ( 63 ) reported that Zn x Mn 1 − X Fe 2 O 4 NPs showed significant anticancer activity against breast cancer. Alafaleq, Zughaibi ( 64 ) proved that Cu-Mn-BNPs have anticancer activity on HT-29 cell line (IC₅₀ 115.2 µg/mL). ZnO-MnO NCs exhibit anticancer activity through diverse mechanisms. They can induce cytotoxicity by generating ROS, leading to oxidative stress and damage to cellular components. Many BNPs trigger apoptosis via activation of caspase cascades and disruption of mitochondrial membrane potential ( 65 ). Some formulations interfere with DNA replication and cell cycle progression, halting proliferation. Certain BNPs also demonstrated enhanced cellular uptake as well as specific target distribution towards cancer cells due to BNPs unique surface properties and tunable compositions, improving therapeutic efficacy and reducing off-target effects compared to monometallic counterparts ( 66 ). Conclusion In conclusion, the innovative and ecological approach for ZnO–MnO NCs mycosynthesis using A. terreus , introduced by the present study, representing a significant improvement superior to the physical and chemical conventional synthetic methods. The biosynthesized ZnO–MnO NCs exhibited well-defined nanoscale features, about 77 nm average crystallite size assessed using XRD, and strong elemental integration as confirmed by EDX (Zn: 17.8 at%, Mn: 4.1 at%, O: 51.4 at%). Functionally, these NCs demonstrated superior antimicrobial activity, with inhibition zones reaching up to 16.25 mm and 15.11 mm towards B. subtilis and E. coli , respectively, outperforming some conventional antibiotics under the same conditions. Furthermore, MTT assay results revealed selective inhibitory effect against breast MCF-7 cancerous cell lines with over 65% cell inhibition at 100 µg/mL. Conversely, the NCs showed less toxic effect against normal cell lines. Compared to other NCs synthesis, the fungal route offers several advantages: lower energy requirements, non-toxic byproducts, and biologically active capping agents that enhance bioactivity. This is the first report, to our knowledge, detailing the biosynthesis of ZnO–MnO NCs via A. terreus with dual antimicrobial and anticancer activities, providing a scalable and sustainable platform for biomedical applications. Future research should explore the molecular mechanisms of action, in vivo efficacy, and potential for formulation into targeted therapeutic systems. Declarations Author contribution: Ebrahim Said, Amr H. Hashem, and Amer M. Abdelaziz conducted the core experimental work, data analysis, and manuscript drafting. Fathy M. Elkady contributed to the experimental design and provided assistance in data interpretation. Ahmed Alhujaily, Samy Selim, Mohammed S. Abdulrahman, Faisal Alsenani, Hiba Shaghaleh, Hattan S. Gattan, Mohammed H. Alruhaili, Mohammed Aufy and Mohanned T. Alharbi participated in manuscript revision critical and technical support. All authors have read and approved the final manuscript. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Data availability: The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request. Acknowledgments: This work was funded by the Deanship of Graduate Studies and Scientific Research at Jouf University under grant No. (DGSSR-2025-FC-01007). Conflicts of Interest: The authors declare no competing interests. References Fareid MA, El-Sherbiny GM, Askar AA, Abdelaziz AM, Hegazy AM, Ab Aziz R et al (2025) Impeding Biofilm-Forming Mediated Methicillin-Resistant Staphylococcus aureus and Virulence Genes Using a Biosynthesized Silver Nanoparticles–Antibiotic Combination. 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J Inorg Organomet Polym Mater 34(10):4950–4960 Alafaleq NO, Zughaibi TA, Jabir NR, Khan AU, Khan MS, Tabrez S (2023) Biogenic Synthesis of Cu-Mn Bimetallic Nanoparticles Using Pumpkin Seeds Extract and Their Characterization and Anticancer Efficacy. Nanomaterials 13(7):1201 Kamli MR, Srivastava V, Hajrah NH, Sabir JSM (2021) Phytogenic Fabrication of Ag-Fe Bimetallic Nanoparticles for Cell Cycle Arrest and Apoptosis Signaling Pathways in Candida auris by. Generating Oxidative Stress. ;10(2) Makada H, Habib S, Singh M (2023) Bimetallic nanoparticles as suitable nanocarriers in cancer therapy. Sci Afr 20:e01700 Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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1","display":"","copyAsset":false,"role":"figure","size":59443,"visible":true,"origin":"","legend":"\u003cp\u003eUV–visible spectrum of mycosynthesized ZnO-MnO NCs and \u003cem\u003eA. terreus\u003c/em\u003e extract.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/adc5522d8afbb8eb32ee0fa8.png"},{"id":100646266,"identity":"18d7bc14-9c95-41a7-9c79-a1454ad89b82","added_by":"auto","created_at":"2026-01-20 04:46:19","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":151150,"visible":true,"origin":"","legend":"\u003cp\u003eFTIR (A) and XRD (B) analysis of biosynthesized ZnO-MnO NCs using \u003cem\u003eA. terreus\u003c/em\u003e.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/478e1fc0a72fe7a772fb1d83.png"},{"id":100796158,"identity":"b7846b30-eb3b-4cd4-9a15-135835bb88d5","added_by":"auto","created_at":"2026-01-21 13:41:07","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":305076,"visible":true,"origin":"","legend":"\u003cp\u003eMycosynthesized ZnO-MnO NCs TEM image (A) and DLS graph (B).\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/3f06cc909917887ed2edd46e.png"},{"id":100646263,"identity":"e14dd121-fe55-46be-beea-d888474a49e5","added_by":"auto","created_at":"2026-01-20 04:46:19","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":227002,"visible":true,"origin":"","legend":"\u003cp\u003eMycosynthesized ZnO-MnO NCs SEM image (A) and EDX graph (B).\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/6d95591e5deb18422fd534eb.png"},{"id":100646268,"identity":"23806faf-6163-4c46-a8d4-1c829d55804a","added_by":"auto","created_at":"2026-01-20 04:46:20","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":584627,"visible":true,"origin":"","legend":"\u003cp\u003eMycosynthesized ZnO-MnO NCs antimicrobial activity demonstrated by well diffusion assay against \u003cem\u003eS. aureus\u003c/em\u003e ATCC25923.\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/05292c4515219b90b7641978.png"},{"id":100646277,"identity":"3dbfeffb-bfa6-414d-b45f-696c9449f07b","added_by":"auto","created_at":"2026-01-20 04:46:20","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":23481,"visible":true,"origin":"","legend":"\u003cp\u003eHeatmap describes the MIC of ZnO-MnO NCs (A) and ceftriaxone (B) towards \u003cem\u003eS. aureus\u003c/em\u003e ATCC 25923 (1), \u003cem\u003eB. subtilis\u003c/em\u003e ATCC 6633 (2), \u003cem\u003eE. coli\u003c/em\u003e ATCC 25922 (3), and \u003cem\u003eK. pneumoniae\u003c/em\u003eATCC 600603 (53).\u003c/p\u003e","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/757e6578ebbc333b65d7dd9f.png"},{"id":100796385,"identity":"22caeb8d-7352-4179-91d7-ebdbc4aa7b71","added_by":"auto","created_at":"2026-01-21 13:42:55","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":49651,"visible":true,"origin":"","legend":"\u003cp\u003eTKA of biosynthesized ZnO-MnO NCs at different concentration towards \u003cem\u003eS. aureus\u003c/em\u003e ATCC 25923.\u003c/p\u003e","description":"","filename":"7.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/8504b5bda49bf86b9f9c0d23.png"},{"id":100646286,"identity":"a640d6d5-abdf-4b3e-87e9-5649ce193ed0","added_by":"auto","created_at":"2026-01-20 04:46:21","extension":"png","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":43482,"visible":true,"origin":"","legend":"\u003cp\u003eTKA of ZnO-MnO NCs at different concentration against \u003cem\u003eB. subtilis\u003c/em\u003e ATCC 6633.\u003c/p\u003e","description":"","filename":"8.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/1be214cdf5d28e0a3503bab0.png"},{"id":100646284,"identity":"e1461e1c-db37-4c42-b1ba-cc8712a1f704","added_by":"auto","created_at":"2026-01-20 04:46:21","extension":"png","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":47144,"visible":true,"origin":"","legend":"\u003cp\u003eTKA of ZnO-MnO NCs at different concentrations against \u003cem\u003eE. coli\u003c/em\u003e ATCC 25922.\u003c/p\u003e","description":"","filename":"9.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/eab00abc6aa61546f7e0e69d.png"},{"id":100646282,"identity":"9beb8003-9721-4bdd-97d9-30f2cf0c04a7","added_by":"auto","created_at":"2026-01-20 04:46:21","extension":"png","order_by":10,"title":"Figure 10","display":"","copyAsset":false,"role":"figure","size":37127,"visible":true,"origin":"","legend":"\u003cp\u003eTKA of ZnO-MnO NCs at different concentration against \u003cem\u003eK. pneumonia \u003c/em\u003eATCC 600603.\u003c/p\u003e","description":"","filename":"10.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/9fba07d6e8770103e5a97b2a.png"},{"id":100646281,"identity":"fc7ee5a1-5879-45e1-8b30-4032e1b23305","added_by":"auto","created_at":"2026-01-20 04:46:20","extension":"png","order_by":11,"title":"Figure 11","display":"","copyAsset":false,"role":"figure","size":162172,"visible":true,"origin":"","legend":"\u003cp\u003eCytotoxic effect of ZnO-MnO NCs against normal (Wi-38) cell line (A), and cancerous (MCF-7) cell line (B).\u003c/p\u003e","description":"","filename":"11.png","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/039e2b19e1f3e8a599267b96.png"},{"id":100857667,"identity":"b3e4bd73-5bf2-4f44-830a-eee13196a340","added_by":"auto","created_at":"2026-01-22 07:18:14","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2839833,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8631439/v1/e28f356b-50c9-4e7c-ac04-3ab0d7e15be5.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eFungal-Mediated Green Synthesis of ZnO–MnO Nanocomposites with Antimicrobial and Anticancer Properties\u003c/strong\u003e\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003eThe rapid spread of developed multidrug-resistant (MDR) microorganisms represent a critical global health threat, significantly undermining the effectiveness of conventional antimicrobial therapies. The MDR pathogenic bacteria including \u003cem\u003eStaphylococcus aureus\u003c/em\u003e (\u003cem\u003eS. aureus\u003c/em\u003e), \u003cem\u003eKlebsiella pneumoniae\u003c/em\u003e (\u003cem\u003eK. pneumoniae\u003c/em\u003e) and \u003cem\u003eE. coli\u003c/em\u003e have gotten resistance to multiple antimicrobial classes with increased difficulty of infections treatment and consequent lengthy diseases, elevated cost of healthcare, as well as increased mortality rates (\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e). Conventional antimicrobials are often insufficient due to the MDR pathogens can form biofilms and express efflux pumps, which protect them from both antibiotics and host immune responses, thereby complicating treatment outcomes (\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eResearchers are actively seeking alternative antimicrobial agents that offer high effectiveness while being more cost-efficient, aiming to overcome limitations of current treatments. Nanotechnology has emerged as a hopeful substitute for conventional antimicrobials in combating MDR bacteria, offering innovative strategies through use of nanoparticles (NPs) with potent antimicrobial properties. Metal oxide NPs generate reactive oxygen species (ROS), disrupt bacterial membranes integrity, and interfere with DNA replication and protein synthesis with consequent exhibition of broad-spectrum antibacterial effects (\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e). Unlike traditional antimicrobials, NPs can concurrently target multiple bacterial components which reduce the development of prospected resistance. Mycosynthesis of NPs represent cost-effective and ecological approach that utilizes fungi to synthesize metallic NPs, as it avoids toxic chemicals and operates under mild conditions by unique biochemical pathways (\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e). These pathways typically involve the metal salts interaction with fungal biomass or extracellular filtrates, with consequent stabilized metal oxide NPs formation through precipitation or thermal decomposition. The mycosynthesized NPs often exhibit enhanced bioactivities including anticancer, antioxidant, and antimicrobial properties, making them attractive for biomedical and agricultural applications (\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e). Zinc oxide NPs eco-friendly method, had superior purity, hexagonal shape (16\u0026ndash;41 nm), and higher antimicrobial, antidiabetic (84.37% α-amylase inhibition), and anticancer activity (notably against MCF-7 cells) due to active phytochemicals like alkaloids (\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e). Manganese oxide (MnO) NPs are biocompatible, with minimal hemolysis and notable antibacterial activity. These NPs have potential inhibitory activity, with about 666.44 \u0026micro;g/mL inhibitory concentration fifty (IC₅₀), against the colorectal HT29 cancer cells and promote the apoptotic process via downregulation Bcl-2 and Bcl-xL anti-apoptotic proteins (\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e). Additionally, quick targeted antimicrobial effects towards phytopathogenic bacteria more than fungi by the mycosynthesized MnO NPs was demonstrated (\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e). Thus, this study aimed for biosynthesis and characterization of ZnO-MnO NCs employing \u003cem\u003eA. terreus\u003c/em\u003e. Also, for evaluation of their antimicrobial efficiency towards numerous bacterial species as well as exploration of their possible anticancer activity.\u003c/p\u003e"},{"header":"Materials and Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eGrowth media, chemicals, and reagents\u003c/h2\u003e \u003cp\u003eCzapek Dox broth (CDB) and Czapek Dox Agar (CDA) media were purchased from Himedia, Cairo, Egypt. Sodium hydroxide (NaOH) precipitating agent, zinc nitrate hexahydrate Zn(NO\u003csub\u003e3\u003c/sub\u003e)\u003csub\u003e2\u003c/sub\u003e.6H\u003csub\u003e2\u003c/sub\u003eO and manganese acetate tetra hydrate (Mn (CH\u003csub\u003e3\u003c/sub\u003eCOO)\u003csub\u003e2\u003c/sub\u003e.4H\u003csub\u003e2\u003c/sub\u003eO) were obtained from Sigma-Aldrich, Germany.\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eMycosynthesis of ZnO-MnO NCs\u003c/h3\u003e\n\u003cp\u003e \u003cem\u003eAspergillus terreus\u003c/em\u003e (OR125572) that was isolated in a previous study (\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e) was used for biosynthesis of ZnO-MnO NCs. Three disks of \u003cem\u003eA. terreus\u003c/em\u003e, each with a diameter of 0.8 cm and cultured for 72 hrs, were used to inoculate CDB fungal medium (100 mL, pH\u0026thinsp;=\u0026thinsp;7) followed by shaking incubation 6 for days at 150 rpm and 30\u0026thinsp;\u0026plusmn;\u0026thinsp;2\u0026deg;C. The \u003cem\u003eA. terreus\u003c/em\u003e biomass was subsequently collected by filtration and rinsed 3 times using distilled water for complete removing of all residual medium constituents. The harvested biomass (10 gm) suspended in distilled water (50 mL) with further shaking incubation (150 rpm) for 48 hrs followed by centrifugation at 7000 rpm to obtain the \u003cem\u003eA. terreus\u003c/em\u003e fungal biomass filtrate (FBF) that served as the biocatalyst for ZnO-MnO NCs synthesis. For this purpose, 3.0 mM of Zn(NO\u003csub\u003e3\u003c/sub\u003e)\u003csub\u003e2\u003c/sub\u003e.6H\u003csub\u003e2\u003c/sub\u003eO and 2.0 mM of Mn(CH\u003csub\u003e3\u003c/sub\u003eCOO)\u003csub\u003e2\u003c/sub\u003e.4H\u003csub\u003e2\u003c/sub\u003eO were added to the FBF (pH\u0026thinsp;=\u0026thinsp;8) with dark shaking incubation (150 rpm) for 24 hrs at 28\u0026thinsp;\u0026plusmn;\u0026thinsp;2\u0026deg;C. The oven was then used for NCs drying for 24 hrs at 120\u0026deg;C (\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e, \u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e).\u003c/p\u003e\n\u003ch3\u003eCharacterization of ZnO-MnO NCs\u003c/h3\u003e\n\u003cp\u003eFormation of ZnO-MnO NCs was initially indicated by the change in FBF color of from yellow to light brown after metal precursors bio-reduction. The UV-visible spectrophotometer (JENWAY 6305, Staffordshire, UK) was then utilized to record the absorbance at wavelengths range of 200\u0026ndash;800 nm. Additionally, the FTIR spectroscopy (Cary-660 model, KBr Pellet technique) was employed for identification of the functional groups on the biosynthesized ZnO-MnO NCs. The TEM was used to analyze particle size and morphology (TEM, JEM-2100 Plus, Jeol, Japan), while DLS provided insights into particle size distribution (Nano ZS, Malvern, UK). Also, the XRD pattern was used for determination of crystalline nature and size (XRD-6000, Shimadzu Scientific Instruments, Japan), while the scanning electron microscopy (SEM) imaging (SEM, ZEISS, EVO-MA10, Germany) revealed surface morphology. Finally, the ZnO-MnO formulation elements and their distribution were examined with spectroscopy based on the energy-dispersive X-ray (EDX) analysis (EDX, Bruker, Germany).\u003c/p\u003e\n\u003ch3\u003eAntimicrobial activity of ZnO-MnO NCs\u003c/h3\u003e\n\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003eWell diffusion assay\u003c/h2\u003e \u003cp\u003eThe inhibition zone diameter (IZD), reflecting the biosynthesized ZnO-MnO NCs antimicrobial activity, was measured against \u003cem\u003eS. aureus\u003c/em\u003e ATCC\u0026reg; 25923, \u003cem\u003eB. subtilis\u003c/em\u003e ATCC\u0026reg; 6633, \u003cem\u003eE. coli\u003c/em\u003e ATCC\u0026reg; 25922, and \u003cem\u003eK. pneumoniae\u003c/em\u003e ATCC\u0026reg; 700603 following the well diffusion assay conducted under restricted aseptic conditions. In brief, prepared suspension of each bacterial isolate of 0.5 McFarland standard was transferred by cotton swab to Muller Hinton agar (MHA) in a way consensus with lawn growth formation. Sterile borer was then used to make 6 wells on the surface of MHA and 100 \u0026micro;L of the mycosynthesized ZnO-MnO NCs (1000 \u0026micro;g/mL), ZnSO\u003csub\u003e4\u003c/sub\u003e (1000 \u0026micro;g/mL), MnSO\u003csub\u003e4\u003c/sub\u003e (1000 \u0026micro;g/mL), fungal filtrate, dimethyl sulfoxide (DMSO) (10% V/V) as negative control, or ceftriaxone (50 \u0026micro;g/mL) standard positive control (PC), all dissolved in DMSO was aseptically added to each well. The plates were shortly incubated for 30 min at 37\u0026deg;C followed by incubation for 24 hrs at 37\u0026deg;C and measurement of IZD formed around the wells (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e). Accordingly, the ZnO-MnO NCs percent inhibition (PI) was calculated using Eq.\u0026nbsp;(1) for the tested strains (\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003cspan class=\"InlineEquation\"\u003e \u003cspan class=\"mathinline\"\u003e\\(\\:\\text{P}\\text{e}\\text{r}\\text{c}\\text{e}\\text{n}\\text{t}\\:\\text{I}\\text{n}\\text{h}\\text{i}\\text{b}\\text{i}\\text{t}\\text{i}\\text{o}\\text{n}\\:\\left(\\text{P}\\text{I}\\right)=\\:\\:\\frac{\\text{I}\\text{Z}\\text{D}\\:\\text{c}\\text{a}\\text{u}\\text{s}\\text{e}\\text{d}\\:\\text{b}\\text{y}\\:\\text{t}\\text{h}\\text{e}\\:\\text{Z}\\text{n}\\text{O}\\:\\text{N}\\text{P}\\text{s}}{\\text{I}\\text{Z}\\text{D}\\:\\text{c}\\text{a}\\text{u}\\text{s}\\text{e}\\text{d}\\:\\text{b}\\text{y}\\:\\text{v}\\text{a}\\text{n}\\text{c}\\text{o}\\text{m}\\text{y}\\text{c}\\text{i}\\text{n}}\\:\\:\\text{X}\\:\\:\\:100\\)\u003c/span\u003e \u003c/span\u003e (Eq.\u0026nbsp;1)\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eQuantitative dilution method\u003c/h2\u003e \u003cp\u003eThe mycosynthesized ZnO-MnO NCs ability for bacterial strains growth suppression was assessed employing broth microdilution method via calculation of the MIC of the NCs as well as its minimum bactericidal concentration (MBC) (\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e). Briefly, ZnO-MnO NCs suspension was serially diluted in Muller Hinton broth (MHB), aseptically in the 96-well microtiter plate raw, with concentration ranged from 512 \u0026micro;g/mL to 16 \u0026micro;g/mL. The 0.5 McFarland standard of the examined bacterial strain (10 \u0026micro;L) inoculated into each well in the raw followed by aerobic incubation for 24 hrs at 37\u0026deg;C. Resazurin indicator was the and incubated for 2 hrs at 37\u0026deg;C. The minimum dose of mycosynthesized ZnO-MnO NCs showing no resazurin blur color change verified as the MIC of the NCs. Samples from wells showing blue color were subsequently transferred to plate of sterile MHA and incubated for 24 hrs at 37\u0026deg;C. The minimum ZnO-MnO NCs concentration showing inhibition of bacterial growth verified as the MBC of the NCs. (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e). The MIC index or tolerance level of bacterial strain to ZnO-MnO NCs determined following Eq.\u0026nbsp;(2).\u003c/p\u003e \u003cp\u003e \u003cspan class=\"InlineEquation\"\u003e \u003cspan class=\"mathinline\"\u003e\\(\\:\\text{T}\\text{o}\\text{l}\\text{e}\\text{r}\\text{a}\\text{n}\\text{c}\\text{e}\\:\\text{l}\\text{e}\\text{v}\\text{e}\\text{l}\\:=\\:\\:\\frac{\\text{M}\\text{B}\\text{C}}{\\text{M}\\text{I}\\text{C}}\\)\u003c/span\u003e \u003c/span\u003e (Eq.\u0026nbsp;2)\u003c/p\u003e \u003cp\u003eConsequently, the results recorded as ZnO-MnO-BNPs bacteriostatic or bactericidal activity at tolerance value ˃ 4 or \u0026le;\u0026thinsp;4, respectively (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eTime-kill kinetics\u003c/h3\u003e\n\u003cp\u003eEach microbial cultures (OD600\u0026thinsp;\u0026gt;\u0026thinsp;0.800) was diluted to about 10\u003csup\u003e6\u003c/sup\u003e colony forming unit (CFU)/mL and treated, in triplicate, with concentrations equivalent to 0.5 MIC, MIC, or 2 MIC of the tested ZnO-MnO NCs, along with ceftriaxone at 50 \u0026micro;g/mL as a PC in tryptic soy broth. During 24 hrs culture incubation at 37\u0026deg;C, aliquots were taken from each treatment at various time intervals and serially diluted in phosphate-buffered saline. The diluted samples inoculated onto tryptic soy agar followed by incubation for 20 hrs at 37\u0026deg;C and calculation of the viable CFU/mL (\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e) .\u003c/p\u003e\n\u003ch3\u003eCytotoxicity\u003c/h3\u003e\n\u003cp\u003eThe cytotoxicity of different biosynthesized ZnO-MnO NCs concentration ranged from 1000 \u0026micro;g/mL to 31.25 \u0026micro;g/mL towards the normal Wi-38 and cancerous MCF-7 cell lines was evaluated following MTT procedures (\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e). The tested cell line was transferred and incubated in 96-well tissue culture plate, the growth medium was decanted, and the formed sheet of monolayer cells was then washed. Afterward, the cells was treated with the mycosynthesized ZnO-MnO NCs serially diluted using RPMI growth medium, untreated cells was used as a control. Following suitable plates incubation, the growth medium was discarded, the gotten formazan crystals dissolved in DMSO, and Finally, the optical density at 560 nm (OD\u003csub\u003e560\u003c/sub\u003e) was determined while the background subtracted at 620 nm for cell amount determination. Finally, the viability and inhibition percent for the tested cell were calculated following Equations (3) and (4), respectively.\u003cdiv id=\"Equa\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equa\" name=\"EquationSource\"\u003e\n$$\\:\\text{V}\\text{i}\\text{a}\\text{b}\\text{i}\\text{l}\\text{i}\\text{t}\\text{y}\\:\\text{%}=\\:\\frac{\\text{T}\\text{r}\\text{e}\\text{a}\\text{t}\\text{m}\\text{e}\\text{n}\\text{t}\\:\\text{O}\\text{D}}{\\text{C}\\text{o}\\text{n}\\text{t}\\text{r}\\text{o}\\text{l}\\:\\text{O}\\text{D}}\\:\\text{X}\\:100\\:\\:\\:\\:\\:\\:\\:\\:\\:\\:\\:\\left(\\text{E}\\text{q}\\text{u}\\text{a}\\text{t}\\text{i}\\text{o}\\text{n}\\:3\\right)$$\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Equb\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equb\" name=\"EquationSource\"\u003e\n$$\\:\\text{I}\\text{n}\\text{h}\\text{i}\\text{b}\\text{i}\\text{t}\\text{i}\\text{o}\\text{n}\\:\\text{%}=100-\\text{V}\\text{i}\\text{a}\\text{b}\\text{i}\\text{l}\\text{i}\\text{t}\\text{y}\\:\\text{%}\\:\\:\\:\\:\\:\\:\\:\\:\\:\\:\\left(\\text{E}\\text{q}\\text{u}\\text{a}\\text{t}\\text{i}\\text{o}\\text{n}\\:4\\right)$$\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eStatistical analysis\u003c/h2\u003e \u003cp\u003eThe primary data was calculated as the mean (M), median, standard error (SE), standard deviation (SD), and relative standard deviation (RSD) employing GraphPad Prism software (v8). Also, it was employed for figures construction and M\u0026thinsp;\u0026plusmn;\u0026thinsp;SD presentation of the findings of the triplicated experiments. As well, the frequencies and percentages, as the calculated descriptive statistics, were applied for investigation of our results.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results and Discussion","content":"\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003eMycosynthesis of ZnO-MnO NCs\u003c/h2\u003e \u003cdiv id=\"Sec14\" class=\"Section3\"\u003e \u003ch2\u003eUV-visible spectroscopy\u003c/h2\u003e \u003cp\u003eThe ZnO-MnO NCs were effectively biosynthesized utilizing the cell-free extracellular filtrate obtained from \u003cem\u003eA. terreus\u003c/em\u003e culture of 6 days old. The biosynthesized NCs optical properties confirmed their nanoscale nature, as evident from a redshift in the absorption bands and characteristic peak observed at 320 nm (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e), as recorded through UV\u0026ndash;vis spectroscopy. The biological synthesis approach for ZnO-MnO NCs has proven to be promising because of its eco-friendliness, cost-effective, rapid, as well as enhanced yield (\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e). Typically, pure ZnO NPs exhibit a strong absorption band around 365\u0026ndash;370 nm, corresponding to ZnO intrinsic band gap transition. In our study, the observed blue shift to 320 nm could attributed to NCs reduced particle size, which leads to quantum confinement effects, as well as the interaction between ZnO and MnO phases within the nanocomposite. Such a shift in the absorption edge has been reported for other bimetallic oxide nanostructures which confirm the successful ZnO\u0026ndash;MnO NCs development and optical properties modification. Also, El-Moslamy et al. (\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e) recorded an absorbance intensity with a peak at 350 nm for MnO NPs. Zhang et al. (\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e) recorded the appearance of characteristic band of 324 nm for AGE-Mn NPs, and Ameen (\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e) detected a peak at 560 nm in Ag-Cu BNPs. Gaber et al. (\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e) found a peak at 375 nm for ZnO-CuO BNPs. Previously, white-rot fungi, including \u003cem\u003ePhanerochaete chrysosporium\u003c/em\u003e, utilized in ZnO NPs biosynthesis (\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e). As well, \u003cem\u003eA. terreus\u003c/em\u003e fungal strain likely secretes various extracellular enzymes, proteins, and other biologically active substances which facilitate NCs biosynthesis (\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e). For instance, Kaur et al. (\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e) revealed ZnO NPs biosynthesis using the endophytic fungus \u003cem\u003eTrichoderma viride\u003c/em\u003e. Similarly, \u003cem\u003ePericonium\u003c/em\u003e sp., another endophytic fungus, was utilized by Ganesan et al. (\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e) for the ZnO NPs sol-gel biosynthesis. Zhang et al. (\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e) recorded MnO NPs mycosynthesis utilizing \u003cem\u003eArcopilus globulus\u003c/em\u003e endophytic fungus. In addition, Abdelrahman et al. (\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e) successfully fabricated Ag-Au NCs through mycosynthesis involving \u003cem\u003eA. fumigatus\u003c/em\u003e and \u003cem\u003eA. flavus\u003c/em\u003e. On a similar note, Sayed et al. (\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e) described the eco-friendly production of Ag-Cu BNPs mediated by \u003cem\u003eS. aureus\u003c/em\u003e. These BNPs show significant potential in various applications, particularly in plant disease management.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003eAnalysis based on FTIR\u003c/h2\u003e \u003cp\u003eThe FTIR of biosynthesized ZnO-MnO NCs using \u003cem\u003eA. terreus\u003c/em\u003e fungal extract illustrated in Fig.\u0026nbsp;(2A). Functional groups present in the fungal metabolites, including amine (-NH\u003csub\u003e2\u003c/sub\u003e), carbonyl (C\u0026thinsp;=\u0026thinsp;O), and hydroxyl (-OH), serve for metal ions active binding and facilitating nucleation and NCs formation (\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e). The FTIR analysis revealed characteristic peaks within wavelength ranged from 400 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e to 4000 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e, including 3718, 2351, 2236, 2150, 2031, 1910, 1568, 1400, 1027, 923, 775, 621, and 456 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e. The characteristic peak of 3718 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e corresponding to hydroxyl (O\u0026ndash;H) groups existence (\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e). Peaks within the 2351\u0026ndash;2031 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e range indicates the stretching of O\u0026thinsp;=\u0026thinsp;C\u0026thinsp;=\u0026thinsp;O and C\u0026thinsp;=\u0026thinsp;C, highlighting the carboxylic and alkene groups occurrence (\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e). These peaks also signify asymmetrical and symmetrical C\u0026ndash;H stretches representing the presence of methyl groups. The specific peak at 1568 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e representing C\u0026ndash;N stretching in amines, while the characteristic peak near 1400 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e suggests aromatic amines existence (\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e). Additionally, the typical peak at 1027 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e correlated to the C\u0026ndash;O (\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e). Furthermore, the peaks present within 500\u0026ndash;1000 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e range are indicative of metal-oxygen bond formation, confirming the successful synthesis of ZnO-MnO NCs (\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec16\" class=\"Section2\"\u003e \u003ch2\u003eAssessment based on XRD\u003c/h2\u003e \u003cp\u003eThe XRD pattern of the mycosynthesized ZnO\u0026ndash;MnO NCs is shown in Fig.\u0026nbsp;(2B). The identified diffraction peaks confirm the coexistence of both ZnO and MnO phases. For ZnO, sharp peaks showed with 2θ of 31.8\u0026deg;, 34.6\u0026deg;, 36.4\u0026deg;, 47.7\u0026deg;, 56.7\u0026deg;, 62.9\u0026deg;, 66.5\u0026deg;, 68.0\u0026deg;, 72.5\u0026deg;, and 77.0\u0026deg; were respectively indexed to the planes (100), (002), (101), (102), (110), (103), (200), (112), (004), and (202) representing the hexagonal wurtzite structure, the findings in accordance with the reference JCPDS card no. 36-1451 and previous results reported by Kamal et al. (\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e) and Motelica et al (\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e). Similarly, Sharma et al. (\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e) identified hexagonal-phase ZnO with reflections at (100), (002), (101), (102), (110), (200), (112), (201), (004), and (202) planes. Modi et al. (\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e) similarly observed comparable peaks for the ZnO-NPs produced by the extracts of onion peel waste, which line up with Bragg's reflections indexed to the planes (002), (101), (102), (110), (103), and (201). Meanwhile, specific peaks observed at 2θ\u0026thinsp;=\u0026thinsp;28.8\u0026deg;, 37.5\u0026deg;, 43.4\u0026deg;, and 56.5\u0026deg; were assigned to the (200), (220), (311), and (400) cubic MnO planes (rock-salt phase), which is consistent with the standard JCPDS card no. 07-0230 (\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e). Detection of well-defined and sharp peaks reflects the ZnO\u0026ndash;MnO NCs high crystallinity. Furthermore, the average crystallite size of the mycosynthesized ZnO\u0026ndash;MnO NCs was approximately 77 nm as determined following Debye\u0026ndash;Scherrer equation, D\u0026thinsp;=\u0026thinsp;Kλ/βcosθ with K\u0026thinsp;=\u0026thinsp;0.9 and Cu-Kα radiation (λ\u0026thinsp;=\u0026thinsp;0.15406 nm) (\u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e39\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec17\" class=\"Section2\"\u003e \u003ch2\u003eExaminations based on TEM and DLS\u003c/h2\u003e \u003cp\u003eThe TEM image illustrates the structural characteristics of ZnO-MnO NCs synthesized via fungal-mediated processes using \u003cem\u003eA. terreus\u003c/em\u003e extract (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eA). The NPs exhibit a layered, sheet-like morphology with overlapping, thin crystalline plates, indicative of well-formed bimetallic oxide structures. This unique morphology not only facilitate the formation of these thin layers but also influence the structural arrangement and uniformity of the NPs (\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e). The presence of multiple, nearly transparent layers suggests that the particles are nanometer-sized, the size was ranged of 50\u0026ndash;100 nm where the average size was 75 nm. Lopez-Ubaldo et al. (\u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e) reported that biosynthesized Ag-Cu BNPs using Ricinus communis exhibited particle sizes of less than 100 nm. Similarly, Sayed et al. (\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e) demonstrated that synthesized Ag, Cu, and their bimetallic NCs were within the nanoscale, with spherical crystals measuring 20 nm, 40 nm, and 80 nm, respectively. Abdelrahman et al. (\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e) described Ag-Au BNPs of 10\u0026ndash;25 nm size, a sphere-like polyhedron morphology and a predominant truncated tetrahedron topographical pattern.\u003c/p\u003e \u003cp\u003eThe particle size determined by DLS (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eB) was larger than that observed in TEM images. This discrepancy arises because the DLS evaluate the NCs hydrodynamic size in solution, that accounts for solvent molecules surrounding the NCs, particle aggregation, overlapping, as well as the electrical double layer creation. In contrast, TEM imaging provides a direct visualization of individual NPs, allowing the exclusion of aggregates from the size analysis (\u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e42\u003c/span\u003e). Our mycosynthesized ZnO-MnO NCs characterized by size range of 80\u0026ndash;110 nm, average size about 99 nm, as well as 0.321 polydispersity index (PDI) which indicate a homogenous colloidal solution. Similarly, Elkhodary et al. (\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e) reported size distribution about 66.52, 77.45, and 76.85 nm for actinomycete filtrate-based biosynthesized B\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e NPs, ZnO NPs, and ZnO-B\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e BNPs, respectively, based on DLS measurements.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec18\" class=\"Section2\"\u003e \u003ch2\u003eInvestigation using SEM and EDX\u003c/h2\u003e \u003cp\u003eThe SEM image (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eA) illustrates the surface morphology of the biosynthesized ZnO\u0026ndash;MnO nanocomposite. The material displays irregular, layered structures with interconnected surfaces, suggesting high crystallinity and particle aggregation. This morphology may enhance surface area and reactivity, making the composite potentially applicable in environmental and biomedical fields (\u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e44\u003c/span\u003e). Elkhodary et al. (\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e) also observed spherical aggregates and foam-like NPs clustering in biosynthesized ZnO\u0026ndash;B\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e composites using fungal filtrate.\u003c/p\u003e \u003cp\u003eThe EDX spectrum (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eB) displays the characteristic peaks of Zn, Mn, O, and C, confirming the successful formation of ZnO\u0026ndash;MnO nanocomposites. Quantitative analysis revealed the presence of 17.8 at% Zn (45.9 wt%), 4.1 at% Mn (8.9 wt%), and 51.4 at% O (32.4 wt%). A minor contribution of carbon (26.7 at%, 12.6 wt%) was also detected, which is likely attributed to residual organic metabolites from the fungal filtrate or the carbon substrate used during SEM sample preparation. The relative abundance of Zn and O is consistent with the presence of ZnO, while the detection of Mn and O supports the coexistence of MnO phases within the nanocomposites. These findings verify the successful biosynthesis of ZnO\u0026ndash;MnO nanocomposites with an integrated elemental composition. EL-Moslamy et al. (\u003cspan citationid=\"CR45\" class=\"CitationRef\"\u003e45\u003c/span\u003e) reported a similar biosynthetic approach for ZnO-MnO NCs, resulting in lateral flake-like structures with varying particle sizes. Elemental analysis in their study showed Zn (60.03%), Mn (2.091%), and O (37.06%), reflecting variability introduced by fungal synthesis conditions. Previous literature also describes diverse morphologies such as cubic ZnO, spherical MnO\u003csub\u003e2\u003c/sub\u003e, and sheet-like or nanorod structures, influenced by precursor type, fungal metabolites, and synthesis methods (\u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e46\u003c/span\u003e, \u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e47\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec19\" class=\"Section2\"\u003e \u003ch2\u003e\u003c/h2\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cb\u003eAntimicrobial activity\u003c/b\u003e of \u003cb\u003eZnO-MnO NCs\u003c/b\u003e\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec20\" class=\"Section2\"\u003e \u003ch2\u003eWell diffusion assay\u003c/h2\u003e \u003cp\u003eThe diffusion method demonstrated the ZnO-MnO NCs (1000 \u0026micro;g/mL) inhibitory effect against a panel of bacterial isolates, as evidenced by the formation of inhibition zones as illustrated in Figs.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e \u0026amp; \u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e. Specifically, \u003cem\u003eB. subtilis\u003c/em\u003e showed the largest IZD (36.66\u0026thinsp;\u0026plusmn;\u0026thinsp;0.58 mm), followed by \u003cem\u003eE. coli\u003c/em\u003e (35\u0026thinsp;\u0026plusmn;\u0026thinsp;0 mm), \u003cem\u003eS. aureus\u003c/em\u003e (29\u0026thinsp;\u0026plusmn;\u0026thinsp;1 mm), and \u003cem\u003eK. pneumoniae\u003c/em\u003e (12.33\u0026thinsp;\u0026plusmn;\u0026thinsp;0.58 mm). When compared to ceftriaxone (50 \u0026micro;g/mL), the ZnO-MnO NCs showed comparable or even superior activity against \u003cem\u003eE. coli\u003c/em\u003e and \u003cem\u003eB. subtilis\u003c/em\u003e, as indicated by the PI values of 108.25% and 105.77%, respectively. However, the NCs were less effective, PI\u0026thinsp;=\u0026thinsp;72.55%, against \u003cem\u003eK. pneumoniae\u003c/em\u003e (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). In comparison to ceftriaxone, the higher action of the NC against \u003cem\u003eE. coli\u003c/em\u003e and \u003cem\u003eB. subtilis\u003c/em\u003e indicate strong inhibitory mechanism that may involve the destruction of bacterial cell membranes and intracellular functions. This is in line with new research showing how metal NPs have antibacterial properties, which frequently include producing ROS and interfering with bacterial enzyme systems (\u003cspan citationid=\"CR48\" class=\"CitationRef\"\u003e48\u003c/span\u003e). The inherent resistance mechanisms, like efflux pumps, may be the cause of the decreased effectiveness against \u003cem\u003eK. pneumoniae\u003c/em\u003e since they can hinder the uptake and action of NPs (\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e49\u003c/span\u003e). The significant inhibition observed against \u003cem\u003eS. aureus\u003c/em\u003e, a clinically relevant pathogen, is particularly noteworthy, as it highlights the potential of ZnO-MnO NCs in combating antimicrobial-resistant strains (\u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e50\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e\u003cimg 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\" width=\"609\" height=\"480\"\u003e\u003c/p\u003e\u003cdiv id=\"Sec21\" class=\"Section2\"\u003e \u003ch2\u003eBroth microdilution assay\u003c/h2\u003e \u003cp\u003eThe MIC of the mycosynthesized ZnO-MnO NCs obtained against a panel of bacterial isolates was in the range of 64\u0026ndash;256 \u0026micro;g/mL (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e). Additionally, the MBC were higher than the MIC, with values of 256 \u0026micro;g/mL for \u003cem\u003eS. aureus\u003c/em\u003e, 128 \u0026micro;g/mL for \u003cem\u003eB. subtilis\u003c/em\u003e, 256 \u0026micro;g/mL for \u003cem\u003eE. coli\u003c/em\u003e, and 512 \u0026micro;g/mL for \u003cem\u003eK. pneumoniae\u003c/em\u003e. Additionally, the findings illustrated a calculated MIC index (MBC/MIC ratio) in the range of 2\u0026ndash;4 indicating a bactericidal effect for all tested isolates (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e). The MIC index of 4 or below is typically indicative of killing rather than mere inhibition (\u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e51\u003c/span\u003e). The observed variability in MIC and MBC values across different bacterial species could be associated with the differences of bacterial wall components and physiological responses to the NCs. The tested \u003cem\u003eB. subtilis\u003c/em\u003e and \u003cem\u003eS. aureus\u003c/em\u003e, Gram-positive bacteria, exhibited lower MICs, potentially resulted from the less complexed bacterial wall, which may facilitate the NCs penetration and consequently enhance their intracellular targets interaction. Conversely, \u003cem\u003eK. pneumoniae\u003c/em\u003e and \u003cem\u003eE. coli\u003c/em\u003e, Gram-negative bacteria complexed with outer bacterial cell membrane, may pose greater barrier to NPs uptake, resulting in higher MICs (\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e52\u003c/span\u003e). The observed efficacy of ZnO-MnO NCs against clinically significant pathogens like \u003cem\u003eS. aureus\u003c/em\u003e and \u003cem\u003eK. pneumoniae\u003c/em\u003e highlights their potential as novel antimicrobial agents, particularly in the face of increasing antimicrobial resistance. Further investigation into the specific mechanisms of action is warranted to fully realize their therapeutic potential.\u003c/p\u003e \u003cp\u003e\u003cimg 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\" width=\"609\" height=\"394\"\u003e\u003c/p\u003e\u003cp\u003e \u003cb\u003eTime-kill assay of ZnO-MnO NCs\u003c/b\u003e \u003c/p\u003e \u003cp\u003eThe mycosynthesized ZnO-MnO NCs' antibacterial activity against \u003cem\u003eS. aureus\u003c/em\u003e was demonstrated by the time-kill experiment, which showed a concentration-dependent decrease in viable bacterial cells over a 24 hrs period. Throughout the experiment, the PC, which was devoid of ZnO-MnO NCs, demonstrated steady bacterial growth and maintained a log CFU of roughly 6. A significant and quick drop in log CFU was seen at treatment with 2 MIC, reaching about 3.5 within 24 hrs, suggesting a strong bactericidal action. The MIC also resulted in a significant reduction in bacterial viability, albeit at a slower rate, reaching a log CFU of approximately 3.5 by 24 hrs. In contrast, the \u0026frac12; MIC treatment exhibited a less pronounced reduction in log CFU, suggesting a diminished bactericidal effect at this lower concentration (Fig.\u0026nbsp;\u003cspan refid=\"Fig7\" class=\"InternalRef\"\u003e7\u003c/span\u003e). Recent research showing prospective metal nanomaterials with strong bacterial inhibitory activity is consistent with the observed time-dependent and concentration-dependent bactericidal activity of ZnO-MnO NCs against \u003cem\u003eS. aureus\u003c/em\u003e. The quick death seen at 2 MIC points to a strong and direct contact between the BNPs and the bacterial cells, possibly including oxidative stress and membrane rupture. This is consistent with findings that demonstrate the ability of metal NPs to induce ROS production with consequent microbial cell membranes disruption and death (\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e) The sustained bactericidal effect observed at MIC, although slower, underscores the potential of ZnO-MnO NCs for sustained antimicrobial activity. The diminished effect at 1/2 MIC highlights the importance of concentration in achieving optimal bactericidal efficacy. The observed efficacy against \u003cem\u003eS. aureus\u003c/em\u003e, a clinically relevant pathogen, is particularly significant in the context of increasing antimicrobial resistance. Several research discovered metal NPs applications to alternative the conventional antimicrobials, particularly against MDR strains (\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e55\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe TKA performed for ZnO-MnO NCs against \u003cem\u003eB. subtilis\u003c/em\u003e ATCC 6633 demonstrated dose-dependent bactericidal effect. At \u0026frac12; MIC, the NCs induced a moderate reduction in bacterial viability, decreasing to 4.5 log CFU/mL over 24 hrs. At the MIC, a more pronounced reduction was observed, with bacterial counts dropping to 3.5 log CFU/mL. The most significant antimicrobial activity was seen at 2 MIC, where the BNPs achieved near-complete eradication (2 log reduction) within 4\u0026ndash;6 hrs, sustaining suppression up to 24 hrs. In Fig.\u0026nbsp;\u003cspan refid=\"Fig8\" class=\"InternalRef\"\u003e8\u003c/span\u003e The 2 MIC concentration\u0026rsquo;s rapid bactericidal effect (12 hrs) suggests that ZnO-MnO NCs disrupt critical bacterial functions, possibly through: interference with metabolic enzymes (Mg\u0026sup2;⁺-dependent pathways in \u003cem\u003eB. subtilis\u003c/em\u003e) (\u003cspan citationid=\"CR56\" class=\"CitationRef\"\u003e56\u003c/span\u003e) or DNA damage due to Zn\u0026sup2;⁺ ion release (\u003cspan citationid=\"CR57\" class=\"CitationRef\"\u003e57\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe TKA demonstrated that ZnO-MnO NCs exhibit concentration-dependent bactericidal activity against \u003cem\u003eE. coli\u003c/em\u003e. At \u0026frac12; MIC, a moderate reduction in bacterial viability was observed, with log CFU decreasing from 6.5 to ~\u0026thinsp;4.5 over 24 hrs. The MIC induced a more significant decline (~\u0026thinsp;3.5 log CFU), while 2 MIC achieved near-complete eradication (~\u0026thinsp;3 log CFU) within 6\u0026ndash;8 hrs, sustaining suppression up to 24 hrs (Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003e). This efficacy in accordance with current studies highlighting the metal NPs microbial inhibitory potential, particularly towards Gram-negative bacteria like \u003cem\u003eE. coli\u003c/em\u003e, often attributed to membrane disruption and intracellular oxidative stress (\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e55\u003c/span\u003e). The observed activity against \u003cem\u003eE. coli\u003c/em\u003e, a common cause of both community-acquired and nosocomial infections, underscores the potential clinical relevance of ZnO-MnO NCs. The diminished bactericidal effect at \u0026frac12; MIC emphasizes the critical role of concentration in achieving optimal antimicrobial activity. Given the increased \u003cem\u003eE. coli\u003c/em\u003e frequency with resistance phenotype, these findings support the exploration of ZnO-MnO NCs as a potential alternative or adjunct therapy.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe TKA revealed a concentration-dependent bactericidal effect of ZnO-MnO NCs against \u003cem\u003eK. pneumoniae\u003c/em\u003e over a 24 hrs period. The PC, devoid of BNPs, exhibited stable bacterial counts, confirming viable growth under the experimental conditions. At exposure to 2 MIC, a rapid and substantial reduction in viable \u003cem\u003eK. pneumoniae\u003c/em\u003e was observed, culminating in a significant decrease in log CFU by 24 hrs, indicating the potent bactericidal activity (Fig.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003e). Current reports discovered possible ZnO-MnO NCs application to alternatives the conventional antimicrobials, particularly against MDR \u003cem\u003eK. pneumonia\u003c/em\u003e (\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e58\u003c/span\u003e)\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cdiv id=\"Sec22\" class=\"Section3\"\u003e \u003ch2\u003eCytotoxicity\u003c/h2\u003e \u003cp\u003eBefore evaluating a new compound for its potential anticancer activity, it's crucial to assess its toxicity towards normal cell lines. This initial step helps determine the compound's general cytotoxicity and selectivity. If a compound exhibits significant toxicity against normal cells, it may not be a suitable candidate for further development as an anticancer agent, as it could cause unacceptable side effects in patients (\u003cspan citationid=\"CR59\" class=\"CitationRef\"\u003e59\u003c/span\u003e). Early evaluating toxicity using normal cell lines at antimicrobial development, could prioritize compounds that demonstrate a more favorable therapeutic window, meaning that the drug less toxic healthy cells than to cancer cells. Our mycosynthesized ZnO-MnO NCs cytotoxicity toward Wi-38 normal cell line shown in Fig.\u0026nbsp;(11A). Several ZnO-MnO NCs doses were evaluated and the results revealed IC₅₀ about 360.7 \u0026micro;g/mL, which confirms its biosafety or non-toxicity (IC₅₀ \u0026ge; 90 \u0026micro;g/mL) (\u003cspan citationid=\"CR60\" class=\"CitationRef\"\u003e60\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eOn the other hand, the mycosynthesized ZnO-MnO NCs malignancy inhibitory ability in case of MCF-7 cell line presented in Fig.\u0026nbsp;(11B) with an IC₅₀ about 106.25 \u0026micro;g/mL. Several earlier reports indicate the BNPs promising antitumor activities towards malignant cells (\u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e61\u003c/span\u003e, \u003cspan citationid=\"CR62\" class=\"CitationRef\"\u003e62\u003c/span\u003e). Zamani-Roudbaraki, Aghajanzadeh-Kiyaseh (\u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e63\u003c/span\u003e) reported that Zn\u003csub\u003ex\u003c/sub\u003e Mn\u003csub\u003e1\u0026thinsp;\u0026minus;\u0026thinsp;X\u003c/sub\u003e Fe\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e NPs showed significant anticancer activity against breast cancer. Alafaleq, Zughaibi (\u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e64\u003c/span\u003e) proved that Cu-Mn-BNPs have anticancer activity on HT-29 cell line (IC₅₀ 115.2 \u0026micro;g/mL). ZnO-MnO NCs exhibit anticancer activity through diverse mechanisms. They can induce cytotoxicity by generating ROS, leading to oxidative stress and damage to cellular components. Many BNPs trigger apoptosis via activation of caspase cascades and disruption of mitochondrial membrane potential (\u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e65\u003c/span\u003e). Some formulations interfere with DNA replication and cell cycle progression, halting proliferation. Certain BNPs also demonstrated enhanced cellular uptake as well as specific target distribution towards cancer cells due to BNPs unique surface properties and tunable compositions, improving therapeutic efficacy and reducing off-target effects compared to monometallic counterparts (\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e66\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e \u003c/div\u003e"},{"header":"Conclusion","content":"\u003cp\u003eIn conclusion, the innovative and ecological approach for ZnO\u0026ndash;MnO NCs mycosynthesis using \u003cem\u003eA. terreus\u003c/em\u003e, introduced by the present study, representing a significant improvement superior to the physical and chemical conventional synthetic methods. The biosynthesized ZnO\u0026ndash;MnO NCs exhibited well-defined nanoscale features, about 77 nm average crystallite size assessed using XRD, and strong elemental integration as confirmed by EDX (Zn: 17.8 at%, Mn: 4.1 at%, O: 51.4 at%). Functionally, these NCs demonstrated superior antimicrobial activity, with inhibition zones reaching up to 16.25 mm and 15.11 mm towards \u003cem\u003eB. subtilis\u003c/em\u003e and \u003cem\u003eE. coli\u003c/em\u003e, respectively, outperforming some conventional antibiotics under the same conditions. Furthermore, MTT assay results revealed selective inhibitory effect against breast MCF-7 cancerous cell lines with over 65% cell inhibition at 100 \u0026micro;g/mL. Conversely, the NCs showed less toxic effect against normal cell lines. Compared to other NCs synthesis, the fungal route offers several advantages: lower energy requirements, non-toxic byproducts, and biologically active capping agents that enhance bioactivity. This is the first report, to our knowledge, detailing the biosynthesis of ZnO\u0026ndash;MnO NCs via \u003cem\u003eA. terreus\u003c/em\u003e with dual antimicrobial and anticancer activities, providing a scalable and sustainable platform for biomedical applications. Future research should explore the molecular mechanisms of action, \u003cem\u003ein vivo\u003c/em\u003e efficacy, and potential for formulation into targeted therapeutic systems.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAuthor contribution:\u003c/strong\u003e Ebrahim Said, Amr H. Hashem, and Amer M. Abdelaziz conducted the core experimental work, data analysis, and manuscript drafting. Fathy M. Elkady contributed to the experimental design and provided assistance in data interpretation. Ahmed Alhujaily, Samy Selim, Mohammed S. Abdulrahman, Faisal Alsenani, Hiba Shaghaleh, Hattan S. Gattan, Mohammed H. Alruhaili, Mohammed Aufy and Mohanned T. Alharbi participated in manuscript revision critical and technical support. All authors have read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate:\u0026nbsp;\u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication:\u0026nbsp;\u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan dir=\"LTR\"\u003eData availability:\u0026nbsp;\u003c/span\u003e\u003c/strong\u003eThe datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments:\u003c/strong\u003e This work was funded by the Deanship of Graduate Studies and Scientific Research at Jouf University under grant No. (DGSSR-2025-FC-01007).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflicts of Interest:\u0026nbsp;\u003c/strong\u003eThe authors declare no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eFareid MA, El-Sherbiny GM, Askar AA, Abdelaziz AM, Hegazy AM, Ab Aziz R et al (2025) Impeding Biofilm-Forming Mediated Methicillin-Resistant Staphylococcus aureus and Virulence Genes Using a Biosynthesized Silver Nanoparticles\u0026ndash;Antibiotic Combination. 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RSC Adv 15(1):513\u0026ndash;523\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZamani-Roudbaraki M, Aghajanzadeh-Kiyaseh M, Shahangian SS, Jashnani S, Khoramabadi H, Shirini F (2024) Breast Cancer Therapy: Harnessing Biocompatible Zinc-manganese Nano-platform for Dual-targeted Photodynamic Therapy. J Inorg Organomet Polym Mater 34(10):4950\u0026ndash;4960\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAlafaleq NO, Zughaibi TA, Jabir NR, Khan AU, Khan MS, Tabrez S (2023) Biogenic Synthesis of Cu-Mn Bimetallic Nanoparticles Using Pumpkin Seeds Extract and Their Characterization and Anticancer Efficacy. Nanomaterials 13(7):1201\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKamli MR, Srivastava V, Hajrah NH, Sabir JSM (2021) Phytogenic Fabrication of Ag-Fe Bimetallic Nanoparticles for Cell Cycle Arrest and Apoptosis Signaling Pathways in Candida auris by. 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Sci Afr 20:e01700\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"University of Vienna","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Mycosynthesis, Aspergillus terreus, ZnO–MnO nanocomposites, Antibacterial activity, Anticancer potential, Eco-friendly synthesis","lastPublishedDoi":"10.21203/rs.3.rs-8631439/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8631439/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe urgent need for sustainable therapeutic nanomaterials has driven interest in green synthesis routes. In this study, zinc oxide\u0026ndash;manganese oxide nanocomposites (ZnO\u0026ndash;MnO NCs) were mycosynthesized using the extracellular filtrate of \u003cem\u003eAspergillus terreus\u003c/em\u003e. Characterization by UV\u0026ndash;visible spectroscopy, FTIR, XRD, TEM, and DLS confirmed their crystalline structure, functional groups, and nanoscale morphology. The NCs showed a distinct absorption at ~\u0026thinsp;320 nm with average particle sizes of 75 nm (TEM) and 99 nm (DLS). Antibacterial assays revealed strong activity against \u003cem\u003eEscherichia coli\u003c/em\u003e and \u003cem\u003eBacillus subtilis\u003c/em\u003e, with inhibition zones surpassing ceftriaxone and MIC values of 64\u0026ndash;256 \u0026micro;g/mL. Time-kill analysis demonstrated rapid bacterial reduction at higher doses. Cytotoxicity studies indicated good safety in normal cells (IC₅₀ = 360.7 \u0026micro;g/mL) and potent anticancer activity against MCF-7 cells (IC₅₀ = 106.25 \u0026micro;g/mL). These findings highlight fungal-mediated biosynthesis as an eco-friendly route for producing ZnO\u0026ndash;MnO NCs with antimicrobial and anticancer potential.\u003c/p\u003e","manuscriptTitle":"Fungal-Mediated Green Synthesis of ZnO–MnO Nanocomposites with Antimicrobial and Anticancer Properties","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-20 04:46:06","doi":"10.21203/rs.3.rs-8631439/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"63c2f24b-dfc2-4a42-b8d1-b01e972900f4","owner":[],"postedDate":"January 20th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":61317823,"name":"Nanoscience"}],"tags":[],"updatedAt":"2026-01-20T04:46:07+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-20 04:46:06","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8631439","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8631439","identity":"rs-8631439","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}