Preparation of pH/temperature smart response hydrogels P(DEA-AMPS-EGDMA) for controlled release of 5-fluorouracil

Preparation of pH/temperature smart response hydrogels P(DEA-AMPS-EGDMA) for controlled release of 5-fluorouracil | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Preparation of pH/temperature smart response hydrogels P(DEA-AMPS-EGDMA) for controlled release of 5-fluorouracil Yalei Wang, Qian Zhang, Ning Qiao, Lihua Liu, Bowu Zhao, Xuepeng Zhang, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7102071/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A temperature/pH-responsive hydrogel drug carrier was synthesized using a simple, convenient and green aqueous free radical polymerization method with N,N-diethylacrylamide (DEA) as the temperature-sensitive monomer, 2-acrylamido-2-methylpropanesulfonic acid (AMPS) as the pH-sensitive monomer, and ethyleneglycol dimethacrylate (EGDMA) as the cross-linking agent, and the structure and properties. The study confirmed its good mechanical, elastic, and swelling properties as well as temperature and pH sensitivity. Then, 5-fluorouracil (5-Fu) was used as a model drug to explore the drug-carrying and drug-releasing properties of this hydrogel, and finally, its biosafety properties were investigated. The results showed that P(DEA-AMPS-EGDMA) had the highest drug loading and encapsulation rate of 326.84 mg.g − 1 and 16.72%, respectively, when the molar ratio of DEA and AMPS was 121 : 9. When the temperature was 37 ℃ and pH was 7.4, the highest cumulative drug release rate was 26.34%, and the drug release process was in accordance with the one-stage release model. The hemolysis rates of P(DEA-AMPS-EGDMA) were all lower than 1.9%, and the cell survival rates were all greater than 87.6%, which indicated that the PDAE hydrogel had good biosafety. Hydrogel Temperature Response pH Response Free Radical Polymerization Biosafety Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7102071","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":499372921,"identity":"a6eb28f1-4679-45b2-80d9-12bea40173f5","order_by":0,"name":"Yalei Wang","email":"","orcid":"","institution":"North China University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Yalei","middleName":"","lastName":"Wang","suffix":""},{"id":499372922,"identity":"3a84aaa6-c36b-4cc5-b2fd-998d971fc53c","order_by":1,"name":"Qian Zhang","email":"","orcid":"","institution":"North China University of Science and 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5-fluorouracil","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Hydrogel, Temperature Response, pH Response, Free Radical Polymerization, 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The study confirmed its good mechanical, elastic, and swelling properties as well as temperature and pH sensitivity. Then, 5-fluorouracil (5-Fu) was used as a model drug to explore the drug-carrying and drug-releasing properties of this hydrogel, and finally, its biosafety properties were investigated. The results showed that P(DEA-AMPS-EGDMA) had the highest drug loading and encapsulation rate of 326.84 mg.g\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e and 16.72%, respectively, when the molar ratio of DEA and AMPS was 121 : 9. When the temperature was 37 ℃ and pH was 7.4, the highest cumulative drug release rate was 26.34%, and the drug release process was in accordance with the one-stage release model. 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