Effects of Self-Esteem on the Associations Between Infertility-Related Stress and Psychological Distress Among Infertile Chinese Women: A Cross-Sectional Study.

Our study used a cross-sectional design, and was conducted in Liaoning Province, China from December 2017 to May 2018. All participants were recruited from the Shenyang Women’s and Children’s Hospital. Participants included women who were diagnosed with infertility, who could communicate fluently in Chinese, and aged over 18 years of age. The exclusion criteria were women who currently suffered from other major diseases, history of psychiatric illness in the past, and cognitive impairments. All eligible participants were invited to participate in the study by their attending physicians. The researchers verified that the participants were well-informed on the purpose and steps involved in this study. After written informed consent was obtained, the participants were requested to complete a structured questionnaire. The minimum sample size was calculated using the formula: n = (Z α/2 ) 2 P (1- P )/δ 2 , using the constants, α=0.05 and Z α/2 =1.96. P is the prevalence of depression and anxiety in Chinese infertile women, which is estimated based on the findings of prior studies. P =0.20 in the current study. 26 δ is tolerable error, and δ=0.04 was used in the present study. A sample size of 384 participants was identified as the minimum sample size required. Overall, 548 eligible participants were included, and 536 effective responses were received (97.8%). Five of the eligible were not able to participate due to health problems, and seven questionnaires that were not complete were excluded. Our study collected information on the demographic variables of patients, including age, residency, educational level, income level, number of times of exercise weekly, sources of stress and life events. Residence was categorized as rural or urban. Educational level was categorized as high school or lower, junior college and bachelor degree or higher. Income was categorized as < 4000 and ≥ 4000 yuan. Number of times of exercise weekly was categorized as 0, 1–2 and 3–4. Sources of stress was categorized as family, oneself and others (friend or colleague). Negative life events referred to whether participants experienced the death of a family member or severe illnesses in the family (eg, cancers), and it was categorized as either yes or no. We also surveyed clinical variables, including causes of infertility and past histories (pregnancy, infection, operation and infertility treatment). Causes of infertility were categorized as male factors (oligospermia, erectile dysfunction, etc.), female factors (eg, endometriosis, ovulation disorders), and unexplained reason. History of infertility treatment was defined as “yes”, if participants had received assisted reproductive technologies (ART). Pregnancy history, operation history and infertility treatment history were categorized as either yes or no. If the participant had experienced sexually transmitted infections or had a history of induced abortion infection, infection history was identified as “yes”. The Hospital Anxiety and Depression Scale (HADS) is one of the most commonly used instruments worldwide for assessing anxiety and depression in a clinical setting in patients with physical problems. 27 The HADS is a 14-item scale that consists of two subscales, including anxiety and depression, with seven items in each. Each of the items is scored on a Likert scale (0 indicates not at all and 3 indicates very much indeed). The global score in each subscale ranges from 0 to 21. A higher score indicates higher levels of anxiety and depression. A HADS-A or HADS-D score of ≥ 8 indicates possible depression and anxiety, and a score of ≥ 10 indicates probable depression and anxiety. 27 Yang et al (2019) speculated that the Chinese version of HADS had good psychometric properties in terms of internal reliability (Cronbach’s α for HADS-D and HADS-A were 0.729 and 0.811, respectively) and structural validity. 28 The Cronbach’s alpha coefficient for HADS was 0.840 (HADS-D: 0.693, HADS-A: 0.779). Newton et al (1999) compiled a Fertility Problem Inventory (FPI), which aimed to measure the degree of stress associated with infertility. FPI contains five dimensions (rejection of a childfree lifestyle, social concern, sexual concern, relationship concern, the need for parenthood), and 46 items. 20 Peng et al (2011) translated FPI from English to Chinese, and reported good validity and reliability for FPI in the Chinese population. 29 Each of the items is scored on a Likert scale (1 indicating “not agree”, while 6 indicating “totally agree”). The global FPI score ranged from 46 to 276. A higher score indicated a higher degree of stress associated with infertility. The Chinese version of FPI produced a Cronbach’s alpha value of 0.810, in a previous study. 29 In this current study, the Cronbach’s α value for the FPI was 0.887. Rosenberg (1965) developed the Rosenberg self-esteem scale (RSES), which was used to determine levels of self-esteem. 30 The Chinese version was translated by Cheng et al (1995) 31 and consists of 10 items. Participants rated each item on a 4-point Likert scale (ranging from “strongly agree” to “strongly disagree”). The version used in this study has also been used in previous studies. 31 , 32 The Chinese version of FPI reported a Cronbach’s alpha value of 0.830 in a previous study. 33 In this study, the Cronbach’s α value for the RSES was 0.749. SPSS 22.0 was used to analyze all statistical data in this study. One-way ANOVA or independent-group t -tests were used to examine the difference between depression and anxiety using categorical variables. Correlations was examined using Pearson’s correlation among continuous variables. Hierarchical regression analysis was used to investigate the effects of independent variables based on depression and anxiety and explore the effect of self-esteem. For model 1, all demographic and clinical variables that were significantly associated with depression or anxiety were included into the univariate analyses. Infertility-related stress was added into model 2; self-esteem was added as a variable into model 3; while the product of infertility-related stress and self-esteem was added into model 4. Self-esteem played a moderating role when the interaction was significant in the regression model. 34 Asymptotic and resampling strategies developed by Preacher and Hayes, 35 were used to examine the mediating role of self-esteem (a*b product) on the association between infertility-related stress and psychological outcomes, including depression and anxiety. The bootstrap estimate was based on 5000 bootstrap samples. The bias-corrected and accelerated 95% confidence interval (BCa 95% CI) was calculated for each a*b product, with a BCa 95% CI excluding 0 indicated a significant mediating role. All statistical tests were two-sided (α=0.05).

Table 1 shows the demographic and infertility characteristics of subjects and the distributions of depression and anxiety. The mean age of the subjects was 32.77 years (SD = 4.42; range, 22–47). Approximately 80% of participants lived in urban areas. Among the 536 infertile women, more than 50% had junior college or higher level of education, and 73.1% had a household monthly income level of ≥ 4000-yuan RMB. Education level, income, sleep disorders, number of times of exercise weekly and history of infertility treatment were associated with depression ( P <0.05). With regard to the level of education and income, patients with a higher level of education and income had a lower score of depression. From among the participants, 315 (58.8%) patients had received infertility treatment and their scores for depression were higher than patients who had not received treatment. Approximately half of the participants (48.7%) suffered from sleep disorders and the level of depression were higher than women without sleep disorders. Patients (46.5%) who did not have a habit of exercising suffered from a higher level of depression than women who exercised. Sleep disorders and a history of infertility treatment were associated with anxiety ( P <0.05). Table 1 Distribution of Characteristics and Univariate Analyses of Depression and Anxiety Variables N ( % ) Depression P Anxiety P Mean ± SD Mean ± SD Age 0.931 0.177  20–29 128(23.9) 5.41±3.41 7.42±3.96  30–39 365(68.1) 5.35±3.43 7.00±3.75  >40 43(8.0) 5.19±3.30 6.21±3.76 Residence 0.419 0.536  Rural area 111(20.7) 5.59±3.21 7.23±3.59  Urban area 425(79.3) 5.29±3.46 6.99±3.80 Education level 0.008 0.395  Senior high school or lower 224(41.8) 5.86±3.38 7.18±3.72  Junior college 114(21.3) 5.25±3.35 6.61±3.86  Bachelor degree or above 198(36.9) 4.83±3.41 7.12±3.74 Income (RMB, yuan) 0.001 0.413  <4000 144(26.9) 6.19±3.29 7.26±3.62  ≥4000 392(73.1) 5.04±3.40 6.96±3.81 History of pregnancy 0.616 0.588  No 283(52.8) 5.28±3.48 6.95±3.81  Yes 253(47.2) 5.43±3.33 7.13±3.70 Cause of infertility 0.116 0.227  Female factor 331(61.8) 5.51±3.20 7.26±3.71  Male factor 93(17.4) 4.69±3.86 6.65±4.10  Unexplained 112(20.9) 5.45±3.58 6.71±3.60 History of infection 0.535 0.878  No 496(92.5) 5.33±3.44 7.03±3.75  Yes 40(7.5) 5.68±3.03 7.13±3.96 History of operation 0.126 0.141  No 316(59.0) 5.17±3.44 6.85±3.75  Yes 220(41.0) 5.60±3.36 7.30±3.77 History of infertility treatment 0.019 0.007  No 221(41.2) 4.94±3.28 6.52±3.52  Yes 315(58.8) 5.64±3.47 7.40±3.88 Negative life event 0.403 0.087  No 404 5.28±3.39 6.88±3.65  Yes 132 5.57±3.47 7.52±4.05 Sleep disorders <0.001 <0.001  No 275(51.3) 4.50±3.23 5.86±3.46  Yes 261(48.7) 6.25±3.37 8.28±3.67 Times of exercise weekly 0.006 0.083  0 249(46.5) 5.86±3.44 7.42±3.82  1–2 188(35.1) 4.95±3.41 6.76±3.66  3–4 99(18.5) 4.85±3.18 6.61±3.72 Sources of stress 0.764 0.095  The family 166(31.0) 5.25±3.48 7.17±3.66  Oneself 293(54.7) 5.34±3.37 6.77±3.81  Others (friend or colleague) 77(14.4) 5.60±3.43 7.78±69 Abbreviation : SD, standard deviation. Distribution of Characteristics and Univariate Analyses of Depression and Anxiety Abbreviation : SD, standard deviation. Table 2 presents levels of depression, anxiety, infertility-related stress, self-esteem and age. The mean score of HADS-D and HADS-A were 5.35 (SD=4.42) and 7.04 (SD=3.76), and ranged from 0 to 16 and from 0 to 18, respectively. The mean values were 140.41 (SD=30.29) and 29.08 (SD=3.94) for PFI and RSES, respectively. In addition, the prevalence of depression was 27.9% (possible depression: 20.3%; probable depression: 7.6%) and anxiety was 42.2% (possible anxiety: 23.5%; probable anxiety: 18.7%) for female infertility. Table 2 Description Statistics for Continuous Variables Variables Mean SD Range n (%) Age 32.77 4.42 22–47 Depression 5.35 3.41 0–16 8 ≤ Scores ≤ 10 109(20.3) 11 ≤ Scores ≤ 21 41(7.6) Anxiety 7.04 3.76 0–18 8 ≤ Scores ≤ 10 126(23.5) 11 ≤ Scores ≤ 21 100(18.7) Infertility-related stress 140.41 30.29 61–226 Self-esteem 29.08 3.94 17–40 Abbreviation : SD, standard deviation. Description Statistics for Continuous Variables Abbreviation : SD, standard deviation. Table 3 demonstrates correlations among infertility-related stress, self-esteem and depression. Infertility-related stress was significantly and positively associated with depression and anxiety ( P <0.01), whereas self-esteem was significantly and negatively associated with depression and anxiety ( P <0.01). Age was also significantly and negatively associated with anxiety ( P <0.05). Table 3 Correlations Among Continuous Variables Variables 1 2 3 4 5 1.Age 1 2.Depression −0.11 1 3.Anxiety −0.103* 0.661** 1 4.Infertility-related stress −0.059 0.456** 0.451** 1 5. Self-esteem 0.163** −0.444** −0.406** −0.445** 1 Notes: * p < 0.05, ** p < 0.01. Correlations Among Continuous Variables Notes: * p < 0.05, ** p < 0.01. Tables 4 and 5 shows the predictors of depression and anxiety based on hierarchical regression analysis. Based on the results of the univariate analyses, age, education, income, sleep disorders, number of times of exercise weekly and history of infertility treatment were defined as control variables in the regression model 1 of depression, and age, sleep disorders and history of infertility treatment were entered into the regression model 1 of anxiety. In model 2, infertility-related stress was significantly and positively associated with depression ( β =0.339, P <0.001) and anxiety ( β =0.401, P <0.001). Self-esteem (depression: β =−0.280, P <0.001; anxiety: β =−0.225, P <0.001) in model 3, together with infertility-related stress, showed 31.4% variance in depression and 31.0% in anxiety. In model 4, interactions were significantly and negatively associated with depression ( F =29.203, β =−0.099, adjusted R 2 =0.322, P <0.001), indicating that the effect of infertility-related stress on depression decreased as the self-esteem of the infertile women increased. For anxiety, the interaction term was significant in model 4 ( F =43.105, β =−0.133, adjusted R 2 =0.321, P <0.001), indicating that the effect of infertility-related stress on anxiety gradually decreased as the self-esteem of the infertile women increased. Table 4 Hierarchical Linear Regression Analyses of the Factors Associated with Depression Variables Depression Model 1( β ) Model 2( β ) Model 3( β ) Model 4( β ) Age 0.023 0.036 0.068 0.064 Education level −0.082 −0.051 −0.006 −0.003 Income −0.134*** −0.093* −0.073* −0.068 History of infertility treatment 0.088* 0.057 0.056 0.053 Sleep disorders 0.245*** 0.188*** 0.167*** 0.167*** Times of exercise weekly −0.106* −0.076* −0.068 −0.069 Infertility-related stress 0.399*** 0.288*** 0.297*** Self-esteem −0.280*** −0.294*** Interaction −0.099** F 11.574 27.275 31.547 29.203 Adjusted R 2 0.106 0.256 0.314 0.322 Notes : * p < 0.05, ** p < 0.01, *** p <0.001. Table 5 Hierarchical Linear Regression Analyses of the Factors Associated with Anxiety Variables Anxiety Model 1( β ) Model 2( β ) Model 3( β ) Model 4( β ) Age −0.084* −0.064 −0.034 −0.039 History of infertility treatment 0.102* 0.069 0.069 0.066 Sleep disorders 0.312*** 0.251*** 0.234*** 0.233*** Infertility-related stress 0.401*** 0.306*** 0.315*** Self-esteem −0.225*** −0.240*** Interaction −0.133** F 24.411 50.783 49.011 43.105 Adjusted R 2 0.116 0.271 0.310 0.321 Notes : * p < 0.05, ** p < 0.01, *** p <0.001. Hierarchical Linear Regression Analyses of the Factors Associated with Depression Notes : * p < 0.05, ** p < 0.01, *** p <0.001. Hierarchical Linear Regression Analyses of the Factors Associated with Anxiety Notes : * p < 0.05, ** p < 0.01, *** p <0.001. As shown in Tables 4 and 5 , infertility-related stress was positively and significantly associated with depression ( β =0.339, P <0.001) and anxiety ( β =0.401, P <0.001) in model 2. In model 3, self-esteem was negatively correlated with depression ( β =−0.280, P <0.001) and anxiety ( β =−0.225, P <0.001) and the effect of infertility-related stress on depression ( β =0.288, P <0.001) and anxiety ( β =0.306, P <0.001) was smaller than that in model 2, indicating that self-esteem may be a partial mediator of the relationship between infertility-related stress with depression and anxiety. Table 6 shows that self-esteem mediated the relationship between infertility-related stress with depression (a*b=0.1109, BCa 95% CI: 0.0770,0.1495) and anxiety (a*b=0.0956, BCa 95% CI: 0.0622,0.1327). Therefore, self-esteem was found to play a partial mediating effect on the relationship between infertility-related stress with depression (c’=0.1133, P <0.001) and anxiety (c’=0.1133, P <0.001). Table 6 Test of Mediation Model X M Y c a b c’ a*b (BCa 95% CI) Infertility-related stress Self-esteem Depression 0.3990*** −0.3962*** −0.2799*** 0.2881*** 0.1109(0.0770,0.1495) Anxiety 0.4014*** −0.4242*** −0.2253*** 0.3058*** 0.0956(0.0622,0.1327) Notes : *** p <0.001. a Assozciations of infertility-related stress with self-esteem. b Associations of self-esteem with depression or anxiety. c Direct associations of infertility-related stress with depression or anxiety. c’ Indirect associations of infertility-related stress with depression or anxiety. BCa 95% CI: Bias-corrected and accelerated 95% Confidence Interval. Age, education level, income, history of infertility treatment, sleep disorders and times of exercise weekly are covariates for depression. Age, history of infertility treatment, sleep disorders are covariates for anxiety. Test of Mediation Model Notes : *** p <0.001. a Assozciations of infertility-related stress with self-esteem. b Associations of self-esteem with depression or anxiety. c Direct associations of infertility-related stress with depression or anxiety. c’ Indirect associations of infertility-related stress with depression or anxiety. BCa 95% CI: Bias-corrected and accelerated 95% Confidence Interval. Age, education level, income, history of infertility treatment, sleep disorders and times of exercise weekly are covariates for depression. Age, history of infertility treatment, sleep disorders are covariates for anxiety.

Infertility is a disease and social problem, which is defined by the World Health Organization (WHO) as “a disease of the reproductive system defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse”, 1 and has been found to affect approximately 10–12% of couples worldwide, 2 with rates being higher (about 31.1%) in low-income and middle-income countries. 3 Among women, 1.9% suffer from primary infertility, while 10.5% experience secondary infertility. 4 In China, relevant large-scale epidemiological studies have revealed that the prevalence of infertility varies widely, ranging from 6.7–25% among women of a reproductive age. 5–7 In addition, prior studies have shown that women with infertility seem to be vulnerable to mental disorders caused by stress related to infertility, compared with males. 8 , 9 The impact of infertility on women’s mental health is profound throughout their life, and includes emotional, spiritual, sexual, and physical aspects. Moreover, infertility can result in low self-esteem, guilt and loneliness, social isolation, and even psychological stress. 10 Depression and anxiety are the most common mental disorders in infertility patients. 11 Studies have found that 14.7% of infertile women suffered from anxiety in an Italian population, while only 4.5% of men were affected. 12 Additionally, some scholars have found increased symptom load where 10–50% of subjects reported mild to moderate depression, 13 while major depression was reported in 17% of women. 14 Psychological disorders in women with infertility are associated with high rates of problematic personality traits, such as neuroticism, 15 and low quality of life. 16 Furthermore, both depression and anxiety can cause a decrease in fertility and rate of pregnancy in patients being treated with assisted reproductive technology. 17 Therefore, the early identification of influencing factors, screening high risk populations and implementing management strategies may be effective in decreasing the negative consequences caused by mental disorders in infertile females. Research has shown that infertility-related stress significantly increases both anxiety and depression in women with infertility. 18 , 19 Infertility-related stress refers to perceived stress from one’s social networks, marital relationship, sexual domain, rejection of a childfree lifestyle, as well as the need for parenthood. 20 In reality, the diagnosis of infertility in females may trigger negative thoughts concerning the importance of the rejection of a childfree lifestyle and the need for parenthood throughout their lives. In addition, it may involve feelings of stigma and social isolation from family and friends, a reduction in sexual pleasure and difficulty in discussing infertility issues with the couple, which induce a higher level of infertility-related stress. Furthermore, infertility treatment leads to heavy psychological and physical stress. Therefore, women with high levels of infertility-related stress can be inclined to suffer from mood disorders. Although the association of both depression and anxiety with infertility-related stress have been examined among female infertility patients, 18 , 19 the underlying causes remain unclear. Over the years, positive psychology has been used increasingly to prevent and treat mental health problems. Self-esteem is a term that reflects a person’s overall evaluation or appraisal of her or his own worth, which encompasses beliefs and emotions, such as triumph, despair, pride or shame. 21 Low levels of self-esteem result in a series of psychological or physical disorders. Self-esteem has been found to be negatively associated with depression among infertile couples. 22 , 23 Additionally, other study have found that self-esteem can mediate the relationship between mindfulness and depression in the general population. 24 Another study found that self-esteem moderated the negative impact of body-related shame and guilt on mental disorders among young adults. 25 Therefore, self-esteem as a protective factor that can effectively buffer against the adverse negative impacts on mental health. In other words, self-esteem may ameliorate the negative effect of infertility-related stress on depression or anxiety. Therefore, our study aimed to ascertain the manner in which self-esteem may affect the associations between infertility-related stress with depression and anxiety in infertile women. Nevertheless, the role of self-esteem on the associations between infertility-related stress with depression and anxiety has still not yet been explored among female infertility patients. Therefore, our study aimed to evaluate the prevalence of depression and anxiety and examine whether self-esteem affects associations of infertility-related stress with depression and anxiety in Chinese women with infertility.

In the study population, the prevalence of depression was 27.9% (possible depression: 20.3%; probable depression: 7.6%) and anxiety was 42.2% (possible anxiety: 23.5%; probable anxiety: 18.7%), which revealed the significantly worse psychological status of infertile women based on depression and anxiety. In addition, the prevalence of comorbidity was 22.4%, indicating that depression and anxiety coexist in Chinese women with infertility. This result was similar to the results of previous studies. 22 , 36 This situation is notable because the comorbidity of depression and anxiety exhibited worse symptoms, lower compliance for treatment and poorer prognosis than patients with a single mental problem. Additionally, compared with other studies, the prevalence of mood disorders in our study population was higher. For instance, Chiaffarino et al (2011) reported that the prevalence of depression and anxiety were 17.9% and 14.7% among women undergoing assisted reproductive treatment at an Italian infertility department in a study conducted on 1000 couples. 12 Biringer et al (2015) found that 17.0% of infertile women suffered from anxiety and 7.0% from depression in study conducted in Norway on 12,584 Norwegian women from 1995 to 1997. 37 In addition, Peterson et al (2014) showed that depression was reported in 11.6% of 1131 infertile women included in a study conducted in Denmark. 38 In this study, the mean score of depression based on HADS-D was 5.35±4.42, which was higher than the results of the study conducted in Italy (3.34±1.65), 39 and the prevalence of anxiety (7.04±3.76) was similar to that of the study conducted in Iran (8.40±4.51). 40 The mean value of infertility-related stress measured using the FPI was 140.41±30.29, which was similar to the results of the study conducted in France (140.81±24.54), 41 and was higher than the that of the study conducted in Italy (134.20±28.96). 42 The average score for self-esteem based on RSES was 29.08 ±3.94, which was higher than that of studies conducted in Egypt (19.55±3.38) 43 and Athens (19.40±4.50). 44 The results of univariate analyses showed that income, education, history of infertility treatment, sleep disorders and exercise were associated with an increase in the prevalence of depression and anxiety. Education was significantly associated with the development of depression, which is in line with the findings of previous studies. 37 , 45 A higher education level was associated with a lower prevalence of depression because women with higher education may have different life roles or other social/work opportunities than only becoming mothers and having to fixate themselves on this life goal. These opportunities may allow them to accept infertility to some extent and cause less distress. A previous study also found that monthly family income was important factor that determined the level of depression. 46 Family income can put an additional strain on the psychological and psychological status of couples struggling with infertility. 47 Improvement in educational level and family income may increase feelings of control and self-confidence in female infertility patients, which relieved their level of depression to some extent. Several articles have reported that (unsuccessful) infertility treatment increases the probability of negative emotions, 48 especially for depression and anxiety. 46 Patients with a higher income had lower scores of depressions in our study. Additionally, a recent study found that poor sleep quality contributed to the development of depression and anxiety in infertile women undergoing in vitro fertilization treatment. 49 Overall, the results of this study showed that after adjusting for control variables, infertility-related stress was an important factor that affected the prevalence of mood disorders in infertile women. More specifically, infertility-related stress was significantly and positively associated with depression and anxiety, which is in accordance with several previous studies. A diagnosis of infertility acts as a negative stressor that increases feelings of stigma and guilt, inducing a series of negative emotions. The stress-health theory suggests that high levels of stress experienced over a long duration could damage an individual’s immune system, and may even lead to psychological and physical vulnerability. 50 Other studies concerning infertility in women have reported that infertility-related stress exhibited a significant effect on psychological distress (eg, depression and anxiety). 18 , 19 Additionally, infertility-related stress can decrease individual well-being and increase marital conflicts. 41 , 51 Noticeably, the mean score of infertility-related stress in our study was higher than that of studies conducted in other countries. 52 , 53 A possible interpretation of this difference may be due to differences in traditional culture. In China, couples are more inclined to give priority to the needs of family members (eg, having babies) over their own interest, and individuals without children are socially marginalized. Therefore, female infertility may be more vulnerable to comments regarding childlessness, and easily induce uncomfortable feelings, resulting in an increase of mental health problems. An increasing number of scholars have become interested in the effects of positive psychological resources on mental health outcomes. Our research study took this research direction to analyze the integrative and independent contributions of these positive psychological effects on depression in a heterogeneous sample of female infertility patients. Self-esteem accounted for a mediating proportion of variance of depression (27.8%) and anxiety (23.8%). Self-esteem, an important inner resource needed for proper psychological and social functioning, was found to be negatively correlated with depression and anxiety, which were consistent with the findings of previous studies. 22 , 23 Moreover, self-esteem was independently associated with infertility-related stress, indicating that each form of positive expectations had an unique association with infertility-related stress. These results contributed to the understanding that self-esteem was a positive resource for combating mental health problems and encouraged us to explore the mediating role of positive psychological resources on the relationship between infertility-related stress with depression and anxiety in this population. In this study, self-esteem played a partial mediating role in the relationship between infertility-related stress with depression and anxiety. In other words, women with fertility who perceived more infertile-related stress were more likely to experience lower levels of positive psychological resources, which in turn increased their possibility of developing depression. Compared with decreasing levels of infertility-related stress, a more positive and strategic method of treatment is to develop programs that increase the inner positive resources of patients, thus enhancing their mental health in the long run. Additionally, our study found that self-esteem moderated the effect of infertility-related stress on depression and anxiety in infertile females. Simple slope analysis showed that when fertile women possessed a high level of self-esteem, levels of depression and anxiety decreased significantly along with high levels of infertility-related stress, compared with low levels of infertility-related stress. In other words, women with fertility possess a high level of self-esteem, and can better maintain mental health even at a higher level of infertility-related stress. Therefore, resilience on self-esteem should be emphasized and developed for individuals to be able to cope with mental health problems.

In summary, our findings revealed that Chinese infertile women had a high prevalence of depression and anxiety. Self-esteem was negatively associated with depression and anxiety. Additionally, self-esteem could affect the effects of infertility-related stress on depression and anxiety. Specifically, self-esteem mediated and moderated the effects of infertility-related stress on depression and anxiety. In addition, infertility-related stress was positively associated with depression and anxiety among Chinese female infertility patients. Thus, interventions that can decrease infertility-related stress and enhance levels of self-esteem should be developed and put applied on Chinese women with infertility.

There are several limitations in this current study. First, our study was of a cross-sectional design. Longitudinal studies are required to replicate our results in further research. Second, these associations in our study may be underestimated or overestimated due to the study being based on self-reported measurement tools. Third, the present study only recruited a non-random sample of infertile women from Liaoning province, which may result in self-selection bias among participants, and limit the generalizability of findings in our study. Finally, the results of our study may be overrated due to the small sample size and high data fluctuations. Furthermore, research conducted in high-income or developed countries showed a lower level of depression and anxiety compared with developing or low-income countries, such as China. People in developed or higher income countries tend to possess adequate health resources and better health literacy to deal with mental health problems.

According to our findings, some valuable implications of this study that will allow for individuals to cope with psychological distress and improve mental health nursing for infertile women should be highlighted. First, there is an urgent need to evaluate patient psychological status during diagnosis and treatment for infertility. Second, psychological counseling should be integrated into in vitro fertilization treatment and other treatment methods to help infertile women cope with mental health problems. Third, family is one of the main sources of stress, and thus, especially spouses should be provided with information on how to be supportive and understanding during mental nursing, which can relieve the negative mood of patients. Finally, self-esteem can mediate and moderate the effects of infertility-related stress on depression and anxiety, as shown in the current study. Therefore, self-esteem is an important positive psychological resource that can reduce stress and improve the mental health of infertile women. Psychological interventions that aim to increase self-esteem should be introduced by clinicians and nurses for women with infertility. Since the building of self-esteem is usually regarded as a dynamic process, interventions such as cognitive-behavioral therapy and mindfulness-based skills can be introduced to infertile women to increase their level of self-esteem, thereby improve mental health. Therefore, targeted intervention approaches should be tested in the next study.