Research of therapeutical effect and immunologic mechanism of Jiawei Foshou San on model rats of endometriosis

Objective: To explore the effect and mechanism of Jiawei Foshou San on the accretion ectopic endometrium of rats.Method: Endometriosis model was established by surgical implant of endometrial tissue which belongs to its body in rats.Jiawei Foshou San was administrated to the model rats.Twenty-eight days later,the length of ectopic endometrium was measured by vernier caliper,the spleen was weighed by electronic balance,the content of tumor necrosis factor(TNF)-α in blood serum and peritoneal fluid was detected by ELISA test,the expression of interleukin(IL)-8 in ectopic endometrium was detected by immunohistochemical method,the expression of NF-κB p65 protein and inhibitory κBα(IκBα) protein in ectopic endometrium were analyzed by western blot.Result: Jiawei Foshou San 0.045,0.09,0.18 g·kg-1group reduced the volume of ectopic endometrium.Jiawei Foshou San 0.18 g·kg-1group raised the spleen exponent of model rats.Jiawei Foshou San 0.09,0.18 g·kg-1group decreased the content of TNF-α in blood serum and peritoneal fluid,and the content of IL-8 in ectopic endometrium was also decreased.Jiawei Foshou San can decrease the expression of NF-κB p65 and increase the expression of IκBα in ectopic endometrium.Conclusion: Jiawei Foshou San can inhibit the accretion of endometriosis implants of rats,and its mechanism might be associated with improving the environment of body.