Uncaria nervosa Elmer, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer

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The results of phytochemical screening contained alkaloids, flavonoids, and terpenoids in the ethanol extract of this plant. Based on literature searches, reports regarding the bioactive compounds responsible for breast cancer have not been found. Further research is needed to understand the potential of Uncaria nervosa Elmer as a breast cancer treatment and to identify the specific compounds responsible for its effects Methods This study aims to determine the metabolite profiling of ethanol extract, the isolation, characterization of bioactive compounds, and their bioactivity in T47D breast cancer cells. The research began by extracting the leaves by maceration using 70% ethanol, and then solid phase extraction was carried out using the solid phase extraction (SPE) method. In this study, the sorbent used was polyamide. The extract was analyzed using a tandem analysis technique based on LCMS using the MZmine and SIRIUS platforms. Isolation was carried out using column chromatography, and preparative recycling HPLC. Bioactive compounds were characterized using UV, HPLC, NMR, and 2D NMR, as well as bioactivity tests using the MTT method. Results The results show that the extract contained N-[(1,3-dimethyl-2,6-dioxo-7-prop-2-ynylpurin-8-yl) amino] formamide, N-(3-phenylbutyl)hexan-2-amine, 1,1-Dichloro-1-nitrosopropane, ceratodictyol, betulinic acid, ursolic acid, 7-methyl-N-[6-[(7-methyl-6-oxooctanoyl) amino] hexyl]-6-oxononanamide, nervisterol and 3,5,10-tris (acetyloxy)-2-hydroxy-4,14,16,16-tetramethyl-8-methylidene-13-oxo-15oxatetracyclo [9.4.1.01,14.04,9] hexadecan-7-yl 3-phenylprop-2-enoate. The ethanol extract of Uncaria nervosa Elmer leaves contains nine compounds consisting of alkaloids, terpenoids, and fatty acid. The bioactive compounds that were successfully isolated were betulinic acid, and ursolic acid, with IC50 values of ˃100 and 14,70±4,50 μg/ml, respectively. These compounds were reported in this plant for the first time. Conclusion Betulinic acid, and ursolic acid have been successfully isolated from leaves Uncaria nervosa Elmer, and ursolic acid have moderate cytotoxic activity on T47D breast cancer cells. " } { "@context": "http://schema.org", "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": "1", "item": { "@id": "https://f1000research.com/", "name": "Home" } }, { "@type": "ListItem", "position": "2", "item": { "@id": "https://f1000research.com/browse/articles", "name": "Browse" } }, { "@type": "ListItem", "position": "3", "item": { "@id": "https://f1000research.com/articles/13-923", "name": "Uncaria nervosa Elmer, a new herbal source for betulinic acid and..." } } ] } Home Browse Uncaria nervosa Elmer, a new herbal source for betulinic acid and... ALL Metrics - Views Downloads Get PDF Get XML Cite How to cite this article Rahmawati N, Ismail NH, Wahyuni FS and Hamidi D. Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.12688/f1000research.152293.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. Close Copy Citation Details Export Export Citation Sciwheel EndNote Ref. Manager Bibtex ProCite Sente EXPORT Select a format first Track Share ▬ ✚ Research Article Revised Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] Noveri Rahmawati https://orcid.org/0009-0000-7746-2009 1 , Nor Hadiani Ismail https://orcid.org/0000-0002-2374-4630 2 , Fatma Sri Wahyuni https://orcid.org/0000-0002-2026-4156 3 , Dachriyanus Hamidi 3 Noveri Rahmawati https://orcid.org/0009-0000-7746-2009 1 , Nor Hadiani Ismail https://orcid.org/0000-0002-2374-4630 2 , Fatma Sri Wahyuni https://orcid.org/0000-0002-2026-4156 3 , Dachriyanus Hamidi 3 PUBLISHED 15 Sep 2025 Author details Author details 1 Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 2 Faculty of Applied sciences, Universiti Teknologi MARA, Shah Alam, Selangor, 68100, Malaysia 3 Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, 25166, Indonesia Noveri Rahmawati Roles: Writing – Original Draft Preparation Nor Hadiani Ismail Roles: Formal Analysis, Methodology Fatma Sri Wahyuni Roles: Methodology, Visualization Dachriyanus Hamidi Roles: Investigation, Writing – Review & Editing OPEN PEER REVIEW DETAILS REVIEWER STATUS This article is included in the Oncology gateway. This article is included in the Plant Science gateway. Abstract Abstract Background Uncaria nervosa Elmer is an Indonesian herbal plant that is traditionally used for breast cancer. The results of phytochemical screening contained alkaloids, flavonoids, and terpenoids in the ethanol extract of this plant. Based on literature searches, reports regarding the bioactive compounds responsible for breast cancer have not been found. Further research is needed to understand the potential of Uncaria nervosa Elmer as a breast cancer treatment and to identify the specific compounds responsible for its effects Methods This study aims to determine the metabolite profiling of ethanol extract, the isolation, characterization of bioactive compounds, and their bioactivity in T47D breast cancer cells. The research began by extracting the leaves by maceration using 70% ethanol, and then solid phase extraction was carried out using the solid phase extraction (SPE) method. In this study, the sorbent used was polyamide. The extract was analyzed using a tandem analysis technique based on LCMS using the MZmine and SIRIUS platforms. Isolation was carried out using column chromatography, and preparative recycling HPLC. Bioactive compounds were characterized using UV, HPLC, NMR, and 2D NMR, as well as bioactivity tests using the MTT method. Results The results show that the extract contained N-[(1,3-dimethyl-2,6-dioxo-7-prop-2-ynylpurin-8-yl) amino] formamide, N-(3-phenylbutyl)hexan-2-amine, 1,1-Dichloro-1-nitrosopropane, ceratodictyol, betulinic acid, ursolic acid, 7-methyl-N-[6-[(7-methyl-6-oxooctanoyl) amino] hexyl]-6-oxononanamide, nervisterol and 3,5,10-tris (acetyloxy)-2-hydroxy-4,14,16,16-tetramethyl-8-methylidene-13-oxo-15oxatetracyclo [9.4.1.0 1 , 14 .0 4 , 9 ] hexadecan-7-yl 3-phenylprop-2-enoate. The ethanol extract of Uncaria nervosa Elmer leaves contains nine compounds consisting of alkaloids, terpenoids, and fatty acid. The bioactive compounds that were successfully isolated were betulinic acid, and ursolic acid, with IC 50 values of ˃100 and 14,70±4,50 μg/ml, respectively. These compounds were reported in this plant for the first time. Conclusion Betulinic acid, and ursolic acid have been successfully isolated from leaves Uncaria nervosa Elmer, and ursolic acid have moderate cytotoxic activity on T47D breast cancer cells. READ ALL READ LESS Keywords Isolation; cytotoxicity; LCMS/MS; T47D; Uncaria nervosa Elmer, MZMine, SIRIUS Corresponding Author(s) Dachriyanus Hamidi ( [email protected] ) Close Corresponding author: Dachriyanus Hamidi Competing interests: No competing interests were disclosed. Grant information: This work was supported by the Ministry of Education, Culture, Research and Technology through DRTPM DIKTI Grant-in-Aid 115/E5/PG.02.00.PL/2023 and derivate contract number by LPPM Unand No. 51/UN16.19/PT.01.03/2023. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Copyright: © 2025 Rahmawati N et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. How to cite: Rahmawati N, Ismail NH, Wahyuni FS and Hamidi D. Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.12688/f1000research.152293.2 ) First published: 15 Aug 2024, 13 :923 ( https://doi.org/10.12688/f1000research.152293.1 ) Latest published: 15 Sep 2025, 13 :923 ( https://doi.org/10.12688/f1000research.152293.2 ) Revised Amendments from Version 1 In revision 2, I have completed the article according to the reviewer's suggestions. I have strengthened the background by adding literature related to the traditional use of plants. In the research methods, the amount of extract used and its yield are shown in Table 1, and an explanation of NMR has also been included in the research methods. A discussion of the use of ethanol extracts and fractions has also been added. The conclusion has been revised according to the reviewer's suggestions. In revision 2, I have completed the article according to the reviewer's suggestions. I have strengthened the background by adding literature related to the traditional use of plants. In the research methods, the amount of extract used and its yield are shown in Table 1, and an explanation of NMR has also been included in the research methods. A discussion of the use of ethanol extracts and fractions has also been added. The conclusion has been revised according to the reviewer's suggestions. See the authors' detailed response to the review by Suhuan Mei See the authors' detailed response to the review by Subiya Showkat READ REVIEWER RESPONSES Introduction Breast cancer is cancer that forms in breast cells. This disease occurs in men and women, but women are more often affected by this disease. Breast cancer usually starts in the ductal carcinoma or lobular carcinoma of the breast. This disease spreads through the lymphatic system or bloodstream. Breast cancer is the most common cancer in women throughout the world, with incidence rates varying in each country. According to the World Health Organization (WHO), there were an estimated 2.3 million new cases of breast cancer diagnosed worldwide in 2020 ( Sung et al., 2021 ). One of the current treatments for breast cancer is chemotherapy. These side effects will be different for each person. The type of drug, dosage, length of time using the drug and the medical history of each individual are factors that greatly influence the side effects that will occur. The cancer treatment continues to develop rapidly and drug development continues, but this disease is still an evolutionary process. Cancer cells can adapt to the treatment given. Many studies have shown that natural products can kill cancer cells and also limit their proliferation by targeting several target molecules and genes in cancer cells ( Newman and Cragg, 2020 ; Zhu et al., 2022 ). Plants are a source of medicinal compounds, due to their ability to synthesize bioactive compounds, especially secondary metabolites ( Joshua et al., 2020 ). Uncaria nervosa Elmer, commonly known as “Akar Kaik Kaik” is a plant species belonging to the genus Uncaria within the family Rubiaceae. This botanical species is indigenous to Southeast Asia, particularly found in regions such as Indonesia, Malaysia, and the Philippines. U. nervosa is renowned for its medicinal properties and has been an integral part of traditional herbal medicine practices in the region for centuries. U. nervosa is a woody vine characterized by its distinctive climbing habit, with slender stems that can reach significant lengths as they twine around trees and other support structures in their natural habitat. The leaves are opposite, elliptical to lanceolate in shape, and possess prominent veins. The plant produces small, tubular flowers with whitish or yellowish hues, which eventually give rise to small fruits containing seeds ( Anonim, n.d. ; Maulina et al., 2019 ). U. nervosa has been revered for its diverse medicinal properties and has been used by indigenous communities for various health purposes. In traditional herbal medicine, different parts of the plant, including the leaves, stems, and roots, are utilized to prepare decoctions, infusions, or poultices to address ailments such as fever, inflammation, digestive disorders, and respiratory conditions. Additionally, U. nervosa has been valued for its purported benefits in promoting overall wellness and vitality. The pharmacological activities of U. nervosa are attributed to its rich phytochemical composition, which includes alkaloids, flavonoids, phenolic compounds, tannins, and triterpenoids ( Ridho, 2023 ). Triterpenoid is a terpenoid, often having a pentacyclic or tetracyclic structure ( Hill and Connolly, 2020 ; Ling et al., 2022 ; Noushahi et al., 2022 ). Pentacyclic triterpenes consist of five isoprene units which are classified into lupane, oleanane, and ursane types ( El-Dawy et al., 2022 ; Kaps et al., 2021 ; Yanlin Li, Jing Wang, Linyong Li, Wenhui Song, Min Li, Xin Hua, Yu Wang, Jifeng Yuan, 2023 ). These compounds have a wide spectrum of pharmacological properties including, cardiovascular activities, antioxidant, antimicrobial, antiviral, antidiabetic, anti-inflammatory, anti-ulcerogenic, anti-obesity, anti-aging, analgesic, immunomodulatory, hypolipidemic, neuroprotective, hepatoprotective, and anticancer ( Adham et al., 2020 ; Chooluck et al., 2021 ; Wang et al., 2020 ). Research on the isolation and cytotoxic activity of U. nervosa has not been widely explored. The cytotoxic activity of ethanol extract of U. nervosa leaves on T47D breast cancer cells has been reported by previous researchers where the IC 50 value was obtained at 64.42 μg/ml ( Rahmawati et al., 2023 ). In this study, the metabolites profiling of ethanol extract was determined using LCMS/MS, isolation using column chromatography and preparative recycling HPLC and cytotoxic testing of T47D breast cancer cells using the MTT method. Methods U. nervosa Elmer plant obtained from the Kampar Forest, Riau, Indonesia. The plant was identified in the ANDA Herbarium, Andalas University, Padang with voucher number NR-04. The chemicals used are ethanol (Merck 1.00983.2500), methanol (Merck 1.06007.4000), acetonitrile (Merck, 1000304000), n-hexane (Merck 1.04367.2500), ethyl acetate (Merck 1.09623.1000), butanol (Merck 1.01990.1000), DMSO, cells T47D, phosphate buffered saline (PBS) (Sigma, P5119), culture media, 20% MTT solution 3-(4,5-dimethylhiazolyl-2,5-diphenyltetrazolium bromide (Merck, M2003). The equipment used were LCMS/MS (Thermo Scientific TM Orbitrap Fushion TM mass spectrometer), recycling preparative HPLC (Japan analytical industry TM ), column Jaigel-ODS-AP.SP-120-15 20x250 mm, liquid nitrogen tank, 96 hole microplate, hemocytometer, biosafety cabinet (Faster), micropipettes (10, 20, 200, 1000 μL), culture flask, 1.5 mL centrifuge tube, inverted/phase contrast microscope (Olympus), centrifugator, CO2 incubator, spectrophotometric microplate reader (Thermo), ELISA reader, Laminar Air Flow and FACS-Calibur. Crude extraction and liquid-liquid fractionation U. nervosa dry leaves (1.932 g) leaves were macerated using the 2000 ml ethanol (Merck 1.00983.2500). The ethanol extract was fractionated using a separating funnel starting with 500 ml n-hexane (Merck 1.04367.2500), 500 ml ethyl acetate (Merck 1.09623.1000), and 500 ml butanol (Merck 1.01990.1000) as solvents. The three fractions were thickened using a rotary evaporator. The ethyl acetate fraction was selected to continue the isolation process. Metabolites profiling of ethanol extract Ultra-High-Performance Liquid Chromatography (UHPLC) The ethanol extract (20 mg) was cleaned up using SPE-polyamide and obtained 5.7 mg (28.5% recovery). SPE is carried out by rinsing using 3 ml methanol (Merck 1.06007.4000) and a conditioning process with water which aims to eliminate interference in the sample (2:98, v/v). The sample was filtered with a 0.45 μm UHPLC filter, then placed in an HPLC vial. The crude was transferred to the Eppendorf tube and dissolved in 1 mL of solvent mixture (80% methanol and 20% water). The tube was sonicated and the solution was filtered (0.22 μm) then put into an HPLC bottle. The stationary phase used was a Luna Omega C18 column (100 x 2.1 mm, 1.6 μm) with a diode array detector (DAD). The sample injected was 1 μL, the column temperature was 30°C, and the system flow rate was 0.28 mL/minute. Elution was carried out using deionized water (% A) and the organic solvent, acetonitrile (Merck, 1000304000), (% B) and solvent system: 0-30 minutes (5-100% B) and 30-40 minutes (100% B) ( Ammar et al., 2024 ). Liquid Chromatography Mass Spectrometry (LCMS) LCMS analysis at a temperature of 30 °C. The sample (1000 ppm), was dissolved in a mixture of methanol and water, then injected 1 μL and the flow rate was set to 0.2 mL/min. LCMS grade water (% A) and methanol (% B) were used in the analysis, with elution systems of 0-30 minutes (10-100% B) and 30-35 minutes (100% B). The samples were filtered using positive ionization mode and analyzed using multistage mass spectrometry (MS/MS) data analysis. The ethanol extract data processing was carried out using the MZmine platform and compound identification using the Sirius platform ( Pang et al., 2024 ; Zheng et al., 2023 ). Isolation and elucidation of bioactive compound The ethyl acetate fraction was weighed as much as 20 g, preabsorbed using silica gel and then put into a chromatography column. The fraction was eluted using a step gradient polarity system solvent, using n-hexane 250 mL (Merck 1.04367.2500), ethyl acetate 250 mL (Merck 1.09623.1000), and methanol 250 mL (Merck 1.06007.4000). There were 11 sub-fractions obtained and the one that was continued for purification was sub-fraction E (HE 5:5). Purification was carried out using recycling preparative HPLC with 1000 ml acetonitrile (Merck, 1000304000) and distilled water as eluents (98:2) ( Ammar et al., 2024 ). Elucidation of the structure of the isolated compound was carried out using a mass spectrometer, 1H- and 1D and 2D-NMR. Samples for NMR (nuclear magnetic resonance) experiments were performed in DMSO solvent, analyzed using NMR Bruker 500 MHz for 1H and NMR 125 MHz for 13 C NMR. Cytotoxic assay against T47D cells Cell lines and culture condition T47D breast cancer cells are a collection of the Cancer Chemoprevention Research Center (CCRC) Gajah Mada University (UGM) Indonesia. T47D cancer cells were taken from the liquid nitrogen tank in a sterile manner, and then the cancer cells in a cyro tube were thawed over a wet bath at a temperature of 37°C for 2-3 minutes. Cancer cells were transferred into a falcon tube containing 9 mL of DMEM and centrifuged at 2000 rpm (10 minutes). The supernatant was removed by pipetting it, leaving a layer of pellets. Next, 2 mL of medium was added to the pellet layer and transferred into a flask, then incubated in a 5% CO 2 incubator at 37°C for 3-4 hours. The flask was observed under an inverted microscope to see whether the cells adhered to the bottom of the flask and formed a monolayer. The growth medium was changed once every two days, and when the number of cells in the flask reached 70-85% confluent, cell sub-culture was carried out. Cell seeding Cell of suspension (180 μL) was made in cell suspension medium, and put into each well except the wells in the first and last columns and the first and last row. The first and last columns, as well as the first and last rows, are blanks, which only contain 200 μL of medium, while the second and seventh columns are controls, which contain 200 μL of cell suspension. Incubate in a 5% CO 2 incubator at 37°C for 24 hours. Cytotoxicity assay Determination of cytotoxic activity was carried out on betulinic acid, ursolic acid and doxorubicin. The 96-well plate, which contains 180 μL of cell suspension and has been incubated for 24 hours, is removed from the incubator. The test solution (20 μL) for each concentration of 0.1-67 μg/mL was transferred into each well except the control well and blank well to obtain a test solution with a concentration of 100, 10, 1, and 0.1 μg/mL. The 96-well plate was again incubated for 48 hours in a 5% CO 2 incubator at 37°C. After that, the medium was discarded, then 100 μL of PBS (Sigma, P5119) was added to each well, and the PBS was removed by pipetting. 100 μL of 0.5 mg/mL MTT (Merck, M2003) solution was pipetted into each well and incubated for 4 hours in a 5% CO 2 incubator at 37°C. After 4 hours, a purple precipitate of formazan crystals will form. Then the cell condition is observed using an inverted microscope. The MTT reagent is discarded, so that what remains is only a purple precipitate of formazan crystals. The precipitate in each well was dissolved in 100 μL of DMSO, and its absorbance was measured using an ELISA reader spectrophotometer at λ 550 nm. Based on the absorbance value between the sample and control cells, the % viability value can be determined. The data is processed using graphPad prism software to obtain the IC 50 value. The cytotoxicity results for the chemicals strong cytotoxicity: IC 50 <4 μg/mL (or IC 50 <10 μM); moderate cytotoxicity: 4 μg/mL< IC 50 <20 μg/mL (or 10 μM< IC 50 <50 μM); low cytotoxicity: 20 μg/mL< IC 50 <100 μg/mL (or 50 μM< IC 50 100 μg/mL (or IC 50 >250 μM) ( Bonsou et al., 2022 ). Statistical analysis Data analysis of extract metabolites profiles and structure elucidation in the form of tables, graphs and images. IC 50 values of samples and controls were determined using statistical analysis GraphPad Prism Software V 9.0.0 (GraphPad Software LLC). Results Crude extraction and liquid-liquid fractionation The leaves of Uncaria nervosa Elmer were extracted using the maceration method. A total of 1,932 g of dried leaves were soaked in 70% ethanol, producing 514 g of extract with a yield of 26.6%. Etanol extract (261.75 g) was further separated using solvents of increasing polarity, n-hexane, ethyl acetate, and butanol and resulting in fractions weighing 10.15 g, 31.34 g, and 19.21 g, respectively ( Table 1 ). Table 1. Yield of ethanol extract and fractions. Sample Yield (%) Ethanol extract 26.60 n-Hexane fraction 3.88 Ethyl acetate fraction 11.97 Butanol fraction 7.34 Metabolites profiling of ethanol extract Determination of the metabolite profile of ethanol extract begins with the clean-up process of ethanol extract using the solid phase extraction (SPE) method. After the clean-up is complete, the extract is prepared for chromatogram determination using UHPLC. The chromatogram profile was monitored under a UV 254 lamp ( Figure 1 ). The extract was prepared to determine the metabolite profile using LCMS. Raw data obtained from the instrument is processed by carrying out data conversion, data processing and metabolite annotation using the Sirius platform. In the ethanol extract, it is predicted that there are 9 compounds ( Figure 2 , Table 2 ), contained N-[(1,3-dimethyl-2,6-dioxo-7-prop-2-ynylpurin-8-yl) amino] formamide, N-(3-phenylbutyl) hexan-2-amine, 1,1-Dichloro-1-nitrosopropane, ceratodictyol, betulinic acid, ursolic acid, 7-methyl-N-[6-[(7-methyl-6-oxooctanoyl) amino] hexyl]-6-oxononanamide, nervisterol and 3,5,10-tris (acetyloxy)-2-hydroxy-4,14,16,16-tetramethyl-8-methylidene-13-oxo-15oxatetracyclo [9.4.1.0 1 , 14 .0 4 , 9 ] hexadecan-7-yl 3-phenylprop-2-enoate. Figure 1. Chromatogram of ethanol extract of U. nervosa leaves at UV 254 nm. Figure 2. Base peak chromatogram of ethanol extract of the leaves of U. nervosa. Table 2. Predicted compounds from the ethanol extract of the leaves of U. nervosa. Peak RT (min) Compound identification Molecular formula Precursor ion (m/z) MS/MS fragmentation Group Sirius similarity (%) 1 1.09 N-[(1,3-dimethyl-2,6-dioxo-7-prop-2-ynylpurin-8-yl) amino] formamide C 11 H 12 N 6 O 3 277.10 139.01, 121.05 Alkaloid 61 2 1.76 N-(3-phenylbutyl) hexan-2-amine C 16 H 27 N 234.22 205.18, 204.17, 190.15, 177.15, 163.13, 176.14, 162.12, 149.11, 148.11 Alkaloid 99 3 2.09 1,1-Dichloro-1-nitrosopropane C 3 H 5 Cl 2 NO 141.98 113.96, 100.95, 97.96 Fatty acid 99 4 7.85 Ceratodictyol C 19 H 38 O 4 331,28 257.24, 239.23, 221.22 Fatty acid 100 5 9.00 Betulinic acid C 30 H 48 O 3 455.33 453.77, 353.67, 99.46 Triterpenoid 70 6 9.57 Ursolic acid C 30 H 48 O 3 455.65 450.54, 407.67, 345.45, 316.83, 187.46 Triterpenoid 100 7 9.86 7-methyl-N-[6-[(7-methyl-6-oxooctanoyl) amino] hexyl]-6-oxononanamide C 25 H 46 N 2 O 4 439.35 394.35, 270.22, 269.22, 244.21, 242.19, 243.20, 241.19 Diterpenoid 100 8 10.66 Nervisterol C 30 H 48 O 425.37 407.36, 131.08, 95.08 Triterpenoid 100 9 11.38 3,5,10-tris (acetyloxy)-2-hydroxy-4,14,16,16-tetramethyl-8-methylidene-13-oxo-15oxatetracyclo[9.4.1.0 1 , 14 .0 4 , 9 ]hexadecan-7-yl 3-phenylprop-2-enoate C 30 H 48 O 12 639.28 621.27, 595.28, 579.26 Triterpenoid 59 Isolation and structural elucidation of the isolated compounds The ethanol extract of U. nervosa Elmer was separated using a fractionation method using n-hexane, ethyl acetate, and butanol as solvents. The isolation process was carried out on the ethyl acetate fraction using column chromatography. The column chromatography results obtained 11 subfractions, and based on monitoring the stain pattern using thin layer chromatography, the isolation was directed to subfraction E. The HPLC chromatogram of subfraction E can be seen in Figure 3 . Figure 3. HPLC profile of U. nervosa Elmer subfraction E. The isolation process for subfraction E was continued using a recycling preparative HPLC instrument, where the subfraction was dissolved in methanol and water using a C-18 column. The results of preparative HPLC recycling obtained six pure compounds ( Figure 4 ), and those that were elucidated were UnE-3 and UnE-4. Compounds Un-E-3 and UnE-4 were monitored by 1H and 13 C NMR, 1D NMR, and 2D NMR ( Figures 5 , 6 , 7 , 8 ). The compounds that have been isolated are betulinic acid (UnE-3) and ursolic acid (UnE-4), with chemical structures as shown in Figure 9 . Figure 4. Six pure compounds from subfraction E. Figure 5. 1H NMR compound UnE-3. Figure 6. 1H NMR compound UnE-4. Figure 7. 13 C NMR compound UnE-3. Figure 8. 13 C NMR compound UnE-4. Figure 9. The structure of betulinic acid (A) and ursolic acid (B). Cytotoxic activity betulinic acid and ursolic acid against T47D cells The cytotoxic activity of betulinic acid (BA) and ursolic acid (UA) on human breast cancer cells (T47D) was determined using the MTT method. As a positive control, the reference standard doxorubicin was used ( Numonov et al., 2020 ). Doxorubicin is one of the most widely used chemotherapy agents in the treatment of breast cancer. The results of the cytotoxic test showed that the IC 50 values for UA and doxorubicin were 14.70 ± 4.50 μg/ml and 0.15 ± 0.02, respectively. The IC 50 value for BA was not determined because the % viability at the highest concentration tested (53 μg/ml) was greater than 50%. The % viability values of UA and doxorubicin can be seen in Figures 10 and 11 . Figure 10. Graph of ursolic acid concentration (μg/ml) and % viability of T47D cells. Figure 11. Graph of doxorubicin concentration (μg/ml) and % viability of T47D cells. Discussion This research began with the preparation of ethanol extract from U. nervosa Elmer leaves. Ethanol was chosen as a solvent because ethanol is a polar solvent (lower polarity than water) so that it can attract bioactive compounds found in U. nervosa Elmer leaves such as alkaloids, flavonoids, and phenolics which can be extracted more optimally with ethanol ( Lin et al., 2020 ). Determining the metabolite profile of the extract begins with clean-up of the ethanol extract using the Solid Phase Extraction method (SPE). The extraction method that can be used for the analysis, separation, and purification of samples in the industrial, pharmaceutical, and toxicological fields. In this research, a cartridge containing a polyamide stationary phase was used. The stages carried out are conditioning, equilibration, sample loading, elution, evaporation of solvent, and yield recovery ( Abd Karim et al., 2022 ; Farré-Segura et al., 2022 ). Samples that have been cleaned up are prepared to determine the UHPLC profile and observed at 254 nm. In the chromatogram, it can be seen that in the ethanol extract, there are polar compounds with higher peaks than semi-polar and non-polar compounds. The metabolite profile of the ethanol extract was determined using LCMS/MS. The ethanol extract data processing was carried out using the MZmine platform and compound identification using the Sirius platform ( Pang et al., 2024 ; Zheng et al., 2023 ). The ethanol extract is predicted to contain 9 compounds consisting of alkaloids, terpenoids, and fatty acids among them N-[(1,3-dimethyl-2,6-dioxo-7-prop-2-ynylpurin-8-yl) amino] formamide, N-(3-phenylbutyl) hexan-2-amine, 1,1-dichloro-1-nitrosopropane, ceratodictyol, betulinic acid, ursolic acid, 7-methyl-N-[6-[(7-methyl-6-oxooctanoyl) amino] hexyl]-6-oxononanamide, nervisterol, and 3,5,10-tris (acetyloxy)-2-hydroxy-4,14,16,16-tetramethyl-8-methylidene-13-oxo-15oxatetracyclo [9.4.1.0 1 , 14 .0 4 , 9 ] hexadecan-7-yl 3-phenylprop-2-enoate. Based on the compounds predicted in the ethanol extract, there are 2 compounds that have been found in other species but have only just been discovered in this species, namely BA and UA. These two compounds are reported to have cytotoxic activity on several cancer cell ( Alam et al., 2021 ; Lou et al., 2021 ). Ethanol extract was fractionated using n-hexane, ethyl acetate and butanol solvents. Furthermore, the isolation process was carried out on the ethyl acetate fraction. The ethyl acetate fraction was chosen because it contains semi-polar compounds, such as flavonoids, phenolics, alkaloids, certain terpenoids, and glycosides. Many bioactive compounds from plants that have the potential to be anticancer are in this polarity range, so they are more widely distributed in the ethyl acetate fraction ( Zhang et al., 2021 ). The separation of ethyl acetate fractions was carried out using column chromatography, a separation technique based on adsorption events. This method allows for the separation of a sample in the form of a mixture weighing several grams ( Koseki et al., 2025 ). Ethyl acetate fraction, preadsorbed using silica gel. The elution system used is an elution system with graded polarity. The eluent comparison starts from non-polar solvents (n-hexane), semi-polar (ethyl acetate) to polar solvents (methanol). The eluent will be increased in polarity if the band that descends with the previous eluent has all been eluted and collected in a vial. The results of column chromatography are collected in a vial and allowed to evaporate. Vials with the same TLC stain pattern are combined and 11 subfractions are obtained which are labeled SF A, SF B, SF C, SF D, SF E, SF F, SF G, SF H, SF I, SF J and SF K. Based on the separation pattern and the number of subfractions available, subfraction E is selected to proceed to the isolation stage. Purification of subfraction E was carried out using a recycling preparative HPLC instrument. The results of the purification of subfraction E, obtained 6 isolates and were labeled UnE-1, UnE-2, UnE-3, UnE-4, UnE-5 and UnE-6 ( Pereda & Gómez, 2024 ). UnE-1, UnE-2, UnE-5 and UnE-6 were obtained in small amounts so that structure elucidation was not carried out. The UnE-3 compound that has been isolated is a white powder. The molecular formula (C 30 H 48 O 3 ) has an M/Z of 456,684 MS (-mode) along with its 1H NMR and 13C NMR spectral data. The compound has a UV spectrum that only has one peak at a maximum absorption of 195.18 nm. This indicates that this compound does not have a conjugated chromophore. To support the UV data, 1D NMR analysis (1H, 13C, and APT NMR in DMSO as well as 2D (HSQC and HMBC) solvent) was carried out. 13C NMR, 125 MHz analysis was carried out to see the total number of carbons. 30 signals were seen, which were divided into 4 types. chemical shift, namely 1 signal at δC 177.72 ppm carbonyl area, 2 signals at δC 150.79 and 110.10 ppm sp2 area, 1 signal at δC 77.26 ppm, and the remaining 26 signals in sp3 area. From the results of the analysis of all spectroscopic data and comparison with the literature, it can be concluded that this compound is classified as a pentacyclic triterpenoid, which has the name betulinic acid ( Rahmawati et al., 2024a ). BA is a pentacyclic triterpenoid compound and in U. nervosa leaves, it is the first to be reported. BA is a naturally occurring compound found primarily in the bark of certain trees, particularly in the white birch tree ( Betula alba ). However, it’s not present in high concentrations in most plants. Apart from white birch, BA has also been found in other plants such as white or silver birch ( Betula pendula ), sweet birch ( Betula lenta ), yellow birch ( Betula alleghaniensis ), white lupin ( Lupinus albus ), Syzygium species ( Pai and Joshi, 2014 ), Paronema canescens ( Muharni et al., 2021 ), Feretia canthioides ( Egbubine et al., 2020 ), and Tetracarpidium conophorum ( Kelly et al., 2021 ). The second compound that has been isolated is UnE-4, which is a white powder and has the molecular formula (C 30 H 48 O 3 ) with m/z 456.66. This compound has a UV spectrum that only has one peak at a maximum absorption of 195.18 nm. This indicates that this compound does not have a conjugated chromophore. Furthermore, to support the UV data, 1D (1H and 13C NMR in DMSO solvent) and 2D (HMBC) NMR analysis was carried out. 13C NMR analysis, 125 MHz was carried out to see the total number of carbons which showed 30 signals which were divided into 4 types of chemical shifts, namely 1 signal at δC 178.75 ppm carbonyl area, 2 signals at δC 138.66 and 125.04 ppm sp2 area, 1 signal at δC 77.29 ppm and the remaining 26 signals in the sp3 area. The 1H-NMR spectrum, 500 MHz there are several specific signals such as two hydroxy protons whose chemical environments are very different, namely at δH 11.93 (s, 1H) which usually bonds directly with the carbon sp2 carbonyl at C-28 while δH 4.29 (d, 1H) possibly binding to the sp3 carbon of methine at C-3. One sp2 area proton signals at δH 5.13 (t, J = 3.25 Hz, 1H) and the rest are aliphatic protons. HMBC data shows a long between H-24 and C-3 which confirms the position of the hydroxy methine which is in ring A. Furthermore, a long between H-27 and C-13 which turns off its position splits rings C and D. Based on Index calculations of Hydrogen Deficiency (IHD) obtained a value of 7 which is in good agreement with NMR data where 5 IHD comes from rings and 2 IHD from double bonds. From the results of the analysis of all spectroscopic data and comparison with the literature, it can be concluded that this compound is classified as a pentacyclic triterpenoid which has the name ursolic acid. UA has reportedly been isolated from several plants including Salvia officinalis ( Jedinák et al., 2006 ), Apples peels ( Yamaguchi et al., 2008 ), Arbutus pavarii ( Groshi et al., 2022 ), and Uncaria macrophylla ( Sun et al., 2012 ). Compounds that have been isolated from the ethanol extract of U. nervosa Elmer leaves are BA and UA. These two compounds were subjected to a cytotoxic test on T47D breast cancer cells using the MTT method and doxorubicin was used as a positive control. The test results show that ursolic acid has strong activity when compared with betulinic acid. The IC 50 values for ursolic acid and doxorubicin were 14.70 ± 4.50 μg/ml and 0.15 ± 0.02, respectively ( Rahmawati et al., 2024b ). BA and UA are the active compounds of pentacyclic triterpenoids which is a triterpene acid that is found in various fruits, vegetables and medicinal plants ( Zafar et al., 2022 ; Maniyamma et al., 2022 ) and has been reported to have antidiabetic, anti-inflammatory, immunomodulatory, and anticancer activities ( Alam et al., 2021 ; Numonov et al., 2020 ; Sianipar, 2021 ; Wang et al., 2020 ). BA and UA are from natural products against metastasis and was also investigated in studies conducted in the last decade ( Khwaza et al., 2020 ; Naeem et al., 2022 ; Zafar et al., 2022 ). Research related to the cytotoxic activity of BA includes the effects of skin carcinogenesis, where it was reported that BA can inhibit 70% of tumor development ( Agame-Lagunes et al., 2021 ). Research on MDA-MB-231 breast cancer cells gave an IC 50 value of 38.81 ± 4.9 mg/mL ( Qi et al., 2021 ). BA was also reported to inhibit the viability of HuCCA and BHK-21 cells and induce neoplastic cell apoptosis ( Phonarknguen et al., 2022 ). Research related to the cytotoxic activity of UA has also been widely reported. UA is active in T47D breast cancer cells with an IC 50 value of 1.63 ± 0.62 μM ( Numonov et al., 2020 ). UA increases cell sensitivity Triple-negative breast cancer (TNBC) to doxorubicin via signaling inactivation ZEB1-AS1/miR-186-5p/ABCC1 ( Lu et al., 2022 ). UA formulated into nanoparticles provides an IC 50 value below 30 μM against pancreatic cancer cells AsPC-1 dan BxPC-3 ( Markowski et al., 2021 ) and UA can significantly increase the drug sensitivity of human breast cancer cells MCF-7/MDA-MB-231 against Epirubicin ( Wang et al., 2022 ). Conclusion BA and UA were successfully isolated and identified for the first time from the leaves of U. nervosa Elmer, an indigenous Indonesian medicinal plant. Metabolite profiling using LC-MS/MS confirmed the presence of nine secondary metabolites, including triterpenoids, alkaloids, and fatty acids. The isolated compound UA demonstrated strong cytotoxic activity against T47D breast cancer cells, with an IC 50 value of 14.70 ± 4.50 μg/mL, while BA showed moderate activity. These findings suggest that U. nervosa Elmer holds significant potential as a novel natural source of pentacyclic triterpenoids with anticancer properties and may contribute to the development of plant-based chemotherapeutic agents. Ethical considerations No animals were harmed in this research. Data availability Zenodo: (Data set) Isolation of Pentacyclic Triterpenoids from The Leaves of Uncaria nervosa Elmer. https://doi.org/10.5281/zenodo.11544271 This project contains the following underlying data: • Dataset of this research Data are available under the terms of the Creative Commons Attribution 4.0 International license (CC4). Zenodo: (Data set) Cytotoxic activity of betulinic acid and ursolic acid againts T47D breast cancer cells. https://zenodo.org/doi/10.5281/zenodo.11545104 This project contains the following underlying data: • Dataset of this research Data are available under the terms of the Creative Commons Attribution 4.0 International license (CCO). 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PubMed Abstract | Publisher Full Text | Free Full Text Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 15 Aug 2024 ADD YOUR COMMENT Comment Author details Author details 1 Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 2 Faculty of Applied sciences, Universiti Teknologi MARA, Shah Alam, Selangor, 68100, Malaysia 3 Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, 25166, Indonesia Noveri Rahmawati Roles: Writing – Original Draft Preparation Nor Hadiani Ismail Roles: Formal Analysis, Methodology Fatma Sri Wahyuni Roles: Methodology, Visualization Dachriyanus Hamidi Roles: Investigation, Writing – Review & Editing Competing interests No competing interests were disclosed. Grant information This work was supported by the Ministry of Education, Culture, Research and Technology through DRTPM DIKTI Grant-in-Aid 115/E5/PG.02.00.PL/2023 and derivate contract number by LPPM Unand No. 51/UN16.19/PT.01.03/2023. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Article Versions (2) version 2 Revised Published: 15 Sep 2025, 13:923 https://doi.org/10.12688/f1000research.152293.2 version 1 Published: 15 Aug 2024, 13:923 https://doi.org/10.12688/f1000research.152293.1 Copyright © 2025 Rahmawati N et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Download Export To Sciwheel Bibtex EndNote ProCite Ref. Manager (RIS) Sente metrics Views Downloads F1000Research - - PubMed Central info_outline Data from PMC are received and updated monthly. - - Citations open_in_new 0 open_in_new 0 open_in_new SEE MORE DETAILS CITE how to cite this article Rahmawati N, Ismail NH, Wahyuni FS and Hamidi D. Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.12688/f1000research.152293.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS track receive updates on this article Track an article to receive email alerts on any updates to this article. TRACK THIS ARTICLE Share Open Peer Review Current Reviewer Status: ? Key to Reviewer Statuses VIEW HIDE Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Version 2 VERSION 2 PUBLISHED 15 Sep 2025 Revised Views 0 Cite How to cite this report: Mei S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414395 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414395 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 23 Oct 2025 Suhuan Mei , Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China Approved VIEWS 0 https://doi.org/10.5256/f1000research.186569.r414395 The authors have thoroughly addressed the reviewers' comments and ... Continue reading READ ALL The authors have thoroughly addressed the reviewers' comments and made improvements in the revised manuscript. I recommend acceptance. Competing Interests: No competing interests were disclosed. Reviewer Expertise: 1. Separation, characterization, and evaluation of bioactive components from foods or plants. 2. The impact of natural products on gut health. 3. Safety evaluation of natural products. 4. Chemical and functional changes of foods during processing I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Mei S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414395 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414395 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Showkat S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414394 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414394 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 16 Oct 2025 Subiya Showkat , Bharathidasan University, Tiruchirappalli, Tamil Nadu, India Approved VIEWS 0 https://doi.org/10.5256/f1000research.186569.r414394 The authors have revised the manuscript as per my previous comments. I ... Continue reading READ ALL The authors have revised the manuscript as per my previous comments. I have no further suggestions, and the manuscript may be considered for indexing. Competing Interests: No competing interests were disclosed. Reviewer Expertise: Phytochemistry, pharmacology, Microbiology and computational studies. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Showkat S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414394 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414394 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Respond or Comment COMMENT ON THIS REPORT Version 1 VERSION 1 PUBLISHED 15 Aug 2024 Views 0 Cite How to cite this report: Mei S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r320017 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-320017 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 07 Mar 2025 Suhuan Mei , Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China Approved with Reservations VIEWS 0 https://doi.org/10.5256/f1000research.167034.r320017 This paper illustrates the cytotoxicity against T47D breast cancer of betulinic acid and ursolic acid from Uncaria nervosa Elmer . The authors have performed LC-MS, UV, HPLC, NMR, and 2D NMR to isolate and characterize bioactive compounds as well as the ... Continue reading READ ALL This paper illustrates the cytotoxicity against T47D breast cancer of betulinic acid and ursolic acid from Uncaria nervosa Elmer . The authors have performed LC-MS, UV, HPLC, NMR, and 2D NMR to isolate and characterize bioactive compounds as well as the MTT method to evaluate the cell viability. The rationale of the proposal is logical and the manuscript is written well but the manuscript should be revised considering the following points which should be incorporated for its improvement. 1. In the title, 'Elmer' does not need italics. 2. The weight of 1.932 g leaves is dry weight or wet weight should be described in the section of methods. Please address the manuscript thoroughly. 3. Details of the NMR should be described in the method section. 4. Is there any data about the yield of the different fractions and pure compounds? 5. Results and discussion have many similar descriptions, for example, “Results Metabolites profiling of ethanol extract Determination of the metabolite profile of ethanol extract begins with the clean-up process of ethanol extract using the solid phase extraction (SPE) method.” “Discussion Metabolites profiling of ethanol extract Determining the metabolite profile of the extract begins with clean-up of the ethanol extract using the Solid Phase Extraction method (SPE).” 6. An acronym of the full name is necessary when they appear first. 7. Please don't use the same subheading in the result and discussion. 8. The sentence 'The ethanol extract data processing was carried out using the MZmine platform and compound identification using the Sirius platform (Pang et al., 2024; Zheng et al., 2023)' in the discussion seems more appropriate when it appears in the method. 9. As mentioned above, please check the manuscript carefully, don’t repeat the result, and add more in-depth discussions. 10. Is the ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer any different compared with other herbal plants? Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: 1. Separation, characterization, and evaluation of bioactive components from foods or plants. 2. The impact of natural products on gut health. 3. Safety evaluation of natural products. 4. Chemical and functional changes of foods during processing I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Mei S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r320017 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-320017 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 19 Mar 2025 Noveri Rahmawati , Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 19 Mar 2025 Author Response Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's ... Continue reading Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's comments. 1 . Thank you. We will revise it. 2 . The weight of 1.932 g leaves is dry weight. We will revise it. Thank you. 3 . We will include the NMR in the method. Thank you. 4 . We have data on the results of different fractions and pure compounds. We will include them in the article. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Thank you. We will revise it. 9 . Thank you. We will revise it. 10 .The ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer on cancer cells is no different when compared to other herbal plants. Betulinic acid and ursolic acid have been reported to have potent bioactivity on several T47D breast cancer cells. Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's comments. 1 . Thank you. We will revise it. 2 . The weight of 1.932 g leaves is dry weight. We will revise it. Thank you. 3 . We will include the NMR in the method. Thank you. 4 . We have data on the results of different fractions and pure compounds. We will include them in the article. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Thank you. We will revise it. 9 . Thank you. We will revise it. 10 .The ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer on cancer cells is no different when compared to other herbal plants. Betulinic acid and ursolic acid have been reported to have potent bioactivity on several T47D breast cancer cells. Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 19 Mar 2025 Noveri Rahmawati , Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 19 Mar 2025 Author Response Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's ... Continue reading Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's comments. 1 . Thank you. We will revise it. 2 . The weight of 1.932 g leaves is dry weight. We will revise it. Thank you. 3 . We will include the NMR in the method. Thank you. 4 . We have data on the results of different fractions and pure compounds. We will include them in the article. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Thank you. We will revise it. 9 . Thank you. We will revise it. 10 .The ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer on cancer cells is no different when compared to other herbal plants. Betulinic acid and ursolic acid have been reported to have potent bioactivity on several T47D breast cancer cells. Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's comments. 1 . Thank you. We will revise it. 2 . The weight of 1.932 g leaves is dry weight. We will revise it. Thank you. 3 . We will include the NMR in the method. Thank you. 4 . We have data on the results of different fractions and pure compounds. We will include them in the article. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Thank you. We will revise it. 9 . Thank you. We will revise it. 10 .The ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer on cancer cells is no different when compared to other herbal plants. Betulinic acid and ursolic acid have been reported to have potent bioactivity on several T47D breast cancer cells. Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Showkat S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r364783 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-364783 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 18 Feb 2025 Subiya Showkat , Bharathidasan University, Tiruchirappalli, Tamil Nadu, India Approved with Reservations VIEWS 0 https://doi.org/10.5256/f1000research.167034.r364783 After carefully reviewing the manuscript and providing my comments, I believe that the manuscript has potential but requires substantial revisions. I recommend that the authors address the issues raised before the manuscript can be reconsidered for indexing. ... Continue reading READ ALL After carefully reviewing the manuscript and providing my comments, I believe that the manuscript has potential but requires substantial revisions. I recommend that the authors address the issues raised before the manuscript can be reconsidered for indexing. Comments for authors: In the introduction, several statements are made about the medicinal properties of U. nervosa , such as its wide range of health benefits and its traditional use by indigenous communities . U. nervosa has been revered for its diverse medicinal properties and has been used by indigenous communities for various health purposes. In traditional herbal medicine, different parts of the plant, including the leaves, stems, and roots, are utilized to prepare decoctions, infusions, or poultices to address ailments such as fever, inflammation, digestive disorders, and respiratory conditions. Additionally, U. nervosa has been valued for its purported benefits in promoting overall wellness and vitality. However, there is no reference provided to support these claims. It would be helpful to include appropriate citations to validate the medicinal properties attributed to the plant. In the methods section, the ethyl acetate fraction is mentioned as being weighed and then subjected to chromatography. However, in the results, it mentions that the ethanol extract was fractionated and then the ethyl acetate fraction was used for isolation. This creates some confusion. Could you clarify whether the isolation was performed on the ethanol extract or the ethyl acetate fraction? Additionally, 20 g of the ethyl acetate fraction was obtained, which seems questionable, as 20 g appears too large. Was this fraction liquid? If so, was it dried before weighing? There are many places in the manuscript where the grammar should be checked. Additionally, there are several sentences that are not well connected. What was the rationale for choosing ethanol extract of U. nervosa for analysis in this study, and how does it compare to other possible extraction methods or solvents in terms of efficiency and bioactivity?" The methodology for NMR studies is missing. The conclusion is very short and not satisfactory. The results and discussion section seems very weak and lacking in depth. Have the authors used a standard for the HPLC analysis? If so, it should be properly labeled in the figure. Ensure all scientific names are italicized throughout the manuscript. Is the work clearly and accurately presented and does it cite the current literature? Partly Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? No If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? No Competing Interests: No competing interests were disclosed. Reviewer Expertise: Phytochemistry and pharmacology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Showkat S. Reviewer Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r364783 ) The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-364783 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 20 Mar 2025 Noveri Rahmawati , Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 20 Mar 2025 Author Response Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's ... Continue reading Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's comments. 1 . Thank you. We will provide references related to the benefits of plants. 2 . Isolation was carried out on the ethyl acetate fraction. Ethanol extract 70% (514 g) was fractionated in stages using n-hexane, ethyl acetate and butanol solvents. The dry ethyl acetate fraction obtained was 20 g. 3 . Thank you. We will revise it. 4 . The basis for selecting ethanol: ethanol is a polar solvent (lower polarity than water) so it can attract bioactive compounds found in U. nervosa elmer leaves such as alkaloids, flavonoids, and phenolics. Comparison with other extraction methods or solvents in terms of efficiency and bioactivity: Extraction using the maceration method is a simple extraction without heating so it is more efficient and allows the bioactive compounds contained in plants to remain stable. The use of other solvents such as water will only be able to attract polar compounds and is less effective for lipophilic compounds. Methanol solvents can extract phenolic compounds more strongly, but are more toxic than ethanol so they are less suitable for biomedical applications. n-hexane and dichloromethane solvents will be effective in attracting non-polar compounds such as essential oils. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Yes, we use standards in HPLC analysis and we will label them correctly in the images. Thank you. 9 . Thank you. We will revise it. Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's comments. 1 . Thank you. We will provide references related to the benefits of plants. 2 . Isolation was carried out on the ethyl acetate fraction. Ethanol extract 70% (514 g) was fractionated in stages using n-hexane, ethyl acetate and butanol solvents. The dry ethyl acetate fraction obtained was 20 g. 3 . Thank you. We will revise it. 4 . The basis for selecting ethanol: ethanol is a polar solvent (lower polarity than water) so it can attract bioactive compounds found in U. nervosa elmer leaves such as alkaloids, flavonoids, and phenolics. Comparison with other extraction methods or solvents in terms of efficiency and bioactivity: Extraction using the maceration method is a simple extraction without heating so it is more efficient and allows the bioactive compounds contained in plants to remain stable. The use of other solvents such as water will only be able to attract polar compounds and is less effective for lipophilic compounds. Methanol solvents can extract phenolic compounds more strongly, but are more toxic than ethanol so they are less suitable for biomedical applications. n-hexane and dichloromethane solvents will be effective in attracting non-polar compounds such as essential oils. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Yes, we use standards in HPLC analysis and we will label them correctly in the images. Thank you. 9 . Thank you. We will revise it. Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 20 Mar 2025 Noveri Rahmawati , Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia 20 Mar 2025 Author Response Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's ... Continue reading Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's comments. 1 . Thank you. We will provide references related to the benefits of plants. 2 . Isolation was carried out on the ethyl acetate fraction. Ethanol extract 70% (514 g) was fractionated in stages using n-hexane, ethyl acetate and butanol solvents. The dry ethyl acetate fraction obtained was 20 g. 3 . Thank you. We will revise it. 4 . The basis for selecting ethanol: ethanol is a polar solvent (lower polarity than water) so it can attract bioactive compounds found in U. nervosa elmer leaves such as alkaloids, flavonoids, and phenolics. Comparison with other extraction methods or solvents in terms of efficiency and bioactivity: Extraction using the maceration method is a simple extraction without heating so it is more efficient and allows the bioactive compounds contained in plants to remain stable. The use of other solvents such as water will only be able to attract polar compounds and is less effective for lipophilic compounds. Methanol solvents can extract phenolic compounds more strongly, but are more toxic than ethanol so they are less suitable for biomedical applications. n-hexane and dichloromethane solvents will be effective in attracting non-polar compounds such as essential oils. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Yes, we use standards in HPLC analysis and we will label them correctly in the images. Thank you. 9 . Thank you. We will revise it. Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's comments. 1 . Thank you. We will provide references related to the benefits of plants. 2 . Isolation was carried out on the ethyl acetate fraction. Ethanol extract 70% (514 g) was fractionated in stages using n-hexane, ethyl acetate and butanol solvents. The dry ethyl acetate fraction obtained was 20 g. 3 . Thank you. We will revise it. 4 . The basis for selecting ethanol: ethanol is a polar solvent (lower polarity than water) so it can attract bioactive compounds found in U. nervosa elmer leaves such as alkaloids, flavonoids, and phenolics. Comparison with other extraction methods or solvents in terms of efficiency and bioactivity: Extraction using the maceration method is a simple extraction without heating so it is more efficient and allows the bioactive compounds contained in plants to remain stable. The use of other solvents such as water will only be able to attract polar compounds and is less effective for lipophilic compounds. Methanol solvents can extract phenolic compounds more strongly, but are more toxic than ethanol so they are less suitable for biomedical applications. n-hexane and dichloromethane solvents will be effective in attracting non-polar compounds such as essential oils. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Yes, we use standards in HPLC analysis and we will label them correctly in the images. Thank you. 9 . Thank you. We will revise it. Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 15 Aug 2024 ADD YOUR COMMENT Comment keyboard_arrow_left keyboard_arrow_right Open Peer Review Reviewer Status info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Reviewer Reports Invited Reviewers 1 2 Version 2 (revision) 15 Sep 25 read read Version 1 15 Aug 24 read read Subiya Showkat , Bharathidasan University, Tiruchirappalli, India Suhuan Mei , Jiangxi Agricultural University, Nanchang, China Comments on this article All Comments (0) Add a comment Sign up for content alerts Sign Up You are now signed up to receive this alert Browse by related subjects keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2025 Mei S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 23 Oct 2025 | for Version 2 Suhuan Mei , Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China 0 Views copyright © 2025 Mei S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions The authors have thoroughly addressed the reviewers' comments and made improvements in the revised manuscript. I recommend acceptance. Competing Interests No competing interests were disclosed. Reviewer Expertise 1. Separation, characterization, and evaluation of bioactive components from foods or plants. 2. The impact of natural products on gut health. 3. Safety evaluation of natural products. 4. Chemical and functional changes of foods during processing I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Mei S. Peer Review Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414395) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414395 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2025 Showkat S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 16 Oct 2025 | for Version 2 Subiya Showkat , Bharathidasan University, Tiruchirappalli, Tamil Nadu, India 0 Views copyright © 2025 Showkat S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (0) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions The authors have revised the manuscript as per my previous comments. I have no further suggestions, and the manuscript may be considered for indexing. Competing Interests No competing interests were disclosed. Reviewer Expertise Phytochemistry, pharmacology, Microbiology and computational studies. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (0) Showkat S. Peer Review Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.186569.r414394) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-923/v2#referee-response-414394 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2025 Mei S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 07 Mar 2025 | for Version 1 Suhuan Mei , Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi, China 0 Views copyright © 2025 Mei S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved With Reservations info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions This paper illustrates the cytotoxicity against T47D breast cancer of betulinic acid and ursolic acid from Uncaria nervosa Elmer . The authors have performed LC-MS, UV, HPLC, NMR, and 2D NMR to isolate and characterize bioactive compounds as well as the MTT method to evaluate the cell viability. The rationale of the proposal is logical and the manuscript is written well but the manuscript should be revised considering the following points which should be incorporated for its improvement. 1. In the title, 'Elmer' does not need italics. 2. The weight of 1.932 g leaves is dry weight or wet weight should be described in the section of methods. Please address the manuscript thoroughly. 3. Details of the NMR should be described in the method section. 4. Is there any data about the yield of the different fractions and pure compounds? 5. Results and discussion have many similar descriptions, for example, “Results Metabolites profiling of ethanol extract Determination of the metabolite profile of ethanol extract begins with the clean-up process of ethanol extract using the solid phase extraction (SPE) method.” “Discussion Metabolites profiling of ethanol extract Determining the metabolite profile of the extract begins with clean-up of the ethanol extract using the Solid Phase Extraction method (SPE).” 6. An acronym of the full name is necessary when they appear first. 7. Please don't use the same subheading in the result and discussion. 8. The sentence 'The ethanol extract data processing was carried out using the MZmine platform and compound identification using the Sirius platform (Pang et al., 2024; Zheng et al., 2023)' in the discussion seems more appropriate when it appears in the method. 9. As mentioned above, please check the manuscript carefully, don’t repeat the result, and add more in-depth discussions. 10. Is the ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer any different compared with other herbal plants? Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise 1. Separation, characterization, and evaluation of bioactive components from foods or plants. 2. The impact of natural products on gut health. 3. Safety evaluation of natural products. 4. Chemical and functional changes of foods during processing I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. reply Respond to this report Responses (1) Author Response 19 Mar 2025 Noveri Rahmawati, Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia Thank you very much for all the comments from the reviewers. We will thoroughly revise this article and send it back to the editor. This is a response to the reviewer's comments. 1 . Thank you. We will revise it. 2 . The weight of 1.932 g leaves is dry weight. We will revise it. Thank you. 3 . We will include the NMR in the method. Thank you. 4 . We have data on the results of different fractions and pure compounds. We will include them in the article. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Thank you. We will revise it. 9 . Thank you. We will revise it. 10 .The ability of betulinic acid and ursolic acid from Uncaria nervosa Elmer on cancer cells is no different when compared to other herbal plants. Betulinic acid and ursolic acid have been reported to have potent bioactivity on several T47D breast cancer cells. View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Mei S. Peer Review Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r320017) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-320017 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2025 Showkat S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 18 Feb 2025 | for Version 1 Subiya Showkat , Bharathidasan University, Tiruchirappalli, Tamil Nadu, India 0 Views copyright © 2025 Showkat S. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved With Reservations info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions After carefully reviewing the manuscript and providing my comments, I believe that the manuscript has potential but requires substantial revisions. I recommend that the authors address the issues raised before the manuscript can be reconsidered for indexing. Comments for authors: In the introduction, several statements are made about the medicinal properties of U. nervosa , such as its wide range of health benefits and its traditional use by indigenous communities . U. nervosa has been revered for its diverse medicinal properties and has been used by indigenous communities for various health purposes. In traditional herbal medicine, different parts of the plant, including the leaves, stems, and roots, are utilized to prepare decoctions, infusions, or poultices to address ailments such as fever, inflammation, digestive disorders, and respiratory conditions. Additionally, U. nervosa has been valued for its purported benefits in promoting overall wellness and vitality. However, there is no reference provided to support these claims. It would be helpful to include appropriate citations to validate the medicinal properties attributed to the plant. In the methods section, the ethyl acetate fraction is mentioned as being weighed and then subjected to chromatography. However, in the results, it mentions that the ethanol extract was fractionated and then the ethyl acetate fraction was used for isolation. This creates some confusion. Could you clarify whether the isolation was performed on the ethanol extract or the ethyl acetate fraction? Additionally, 20 g of the ethyl acetate fraction was obtained, which seems questionable, as 20 g appears too large. Was this fraction liquid? If so, was it dried before weighing? There are many places in the manuscript where the grammar should be checked. Additionally, there are several sentences that are not well connected. What was the rationale for choosing ethanol extract of U. nervosa for analysis in this study, and how does it compare to other possible extraction methods or solvents in terms of efficiency and bioactivity?" The methodology for NMR studies is missing. The conclusion is very short and not satisfactory. The results and discussion section seems very weak and lacking in depth. Have the authors used a standard for the HPLC analysis? If so, it should be properly labeled in the figure. Ensure all scientific names are italicized throughout the manuscript. Is the work clearly and accurately presented and does it cite the current literature? Partly Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? No If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? No Competing Interests No competing interests were disclosed. Reviewer Expertise Phytochemistry and pharmacology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above. reply Respond to this report Responses (1) Author Response 20 Mar 2025 Noveri Rahmawati, Doctoral Program, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia Thank you for all the suggestions for our article. We will revise the article thoroughly and send it back to the editor. Herewith we convey some responses to the reviewer's comments. 1 . Thank you. We will provide references related to the benefits of plants. 2 . Isolation was carried out on the ethyl acetate fraction. Ethanol extract 70% (514 g) was fractionated in stages using n-hexane, ethyl acetate and butanol solvents. The dry ethyl acetate fraction obtained was 20 g. 3 . Thank you. We will revise it. 4 . The basis for selecting ethanol: ethanol is a polar solvent (lower polarity than water) so it can attract bioactive compounds found in U. nervosa elmer leaves such as alkaloids, flavonoids, and phenolics. Comparison with other extraction methods or solvents in terms of efficiency and bioactivity: Extraction using the maceration method is a simple extraction without heating so it is more efficient and allows the bioactive compounds contained in plants to remain stable. The use of other solvents such as water will only be able to attract polar compounds and is less effective for lipophilic compounds. Methanol solvents can extract phenolic compounds more strongly, but are more toxic than ethanol so they are less suitable for biomedical applications. n-hexane and dichloromethane solvents will be effective in attracting non-polar compounds such as essential oils. 5 . Thank you. We will revise it. 6 . Thank you. We will revise it. 7 . Thank you. We will revise it. 8 . Yes, we use standards in HPLC analysis and we will label them correctly in the images. Thank you. 9 . Thank you. We will revise it. View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Showkat S. Peer Review Report For: Uncaria nervosa Elme r, a new herbal source for betulinic acid and ursolic acid: Metabolites profiling, isolation, and in vitro cytotoxicity studies against T47D breast cancer [version 2; peer review: 2 approved] . F1000Research 2025, 13 :923 ( https://doi.org/10.5256/f1000research.167034.r364783) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-923/v1#referee-response-364783 Alongside their report, reviewers assign a status to the article: Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. 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