Engineering Saccharomyces cerevisiae with Novel Functional Xylose Isomerases from Rumen Microbiota for Enhanced Biofuel Production

Engineering Saccharomyces cerevisiae with Novel Functional Xylose Isomerases from Rumen Microbiota for Enhanced Biofuel Production | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Biotechnology Journal This is a preprint and has not been peer reviewed. Data may be preliminary. 3 March 2025 V1 Latest version Share on Engineering Saccharomyces cerevisiae with Novel Functional Xylose Isomerases from Rumen Microbiota for Enhanced Biofuel Production Authors : Beatriz de Oliveira Vargas , Marcelo Falsarella Carazzolle , Juliana Pimentel Galhardo , Juliana José , Brenda Cristina de Souza , Jéssica Batista de Lima Correia , Jade Ribeiro dos Santos , Gonçalo Pereira 0000-0003-4140-3482 [email protected] , and Fellipe da Silveira Bezerra de Mello 0000-0001-5842-1682 Authors Info & Affiliations https://doi.org/10.22541/au.174100785.54855403/v1 Published Biotechnology Journal Version of record Peer review timeline 456 views 193 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Metagenomic and metatranscriptomic analysis of rumen microbiota led to the discovery of five Bacillota xylose isomerases (XIs) enabling efficient xylose-to-ethanol conversion in S. cerevisiae . One XI from sheep rumen achieved 90% ethanol yield in 72h, while another from camel rumen showed high substrate affinity (KM = 16.25 mM). These top-performing XIs, the first mined from these sources, could accelerate second-generation biofuel commercialization. Supplementary Material File (main vargas et al 2025 (xi) biot.docx) Download 2.59 MB Information & Authors Information Version history V1 Version 1 03 March 2025 Peer review timeline Published Biotechnology Journal Version of Record 9 Jun 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Biotechnology Journal Keywords bioenergy fermentation genetic engineering genetics industrial biotechnology metabolic engineering metagenomics saccharomyces cerevisiae second generation ethanol xylose isomerase yeast Authors Affiliations Beatriz de Oliveira Vargas Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Marcelo Falsarella Carazzolle Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Juliana Pimentel Galhardo Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Juliana José Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Brenda Cristina de Souza Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Jéssica Batista de Lima Correia Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Jade Ribeiro dos Santos Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Gonçalo Pereira 0000-0003-4140-3482 [email protected] Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Fellipe da Silveira Bezerra de Mello 0000-0001-5842-1682 Universidade Estadual de Campinas Departamento de Genetica Evolucao Microbiologia e Imunologia View all articles by this author Metrics & Citations Metrics Article Usage 456 views 193 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Beatriz de Oliveira Vargas, Marcelo Falsarella Carazzolle, Juliana Pimentel Galhardo, et al. Engineering Saccharomyces cerevisiae with Novel Functional Xylose Isomerases from Rumen Microbiota for Enhanced Biofuel Production. Authorea . 03 March 2025. DOI: https://doi.org/10.22541/au.174100785.54855403/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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