Clinical Assessments and Gait Performance in Patients with Trimalleolar Fractures

Clinical Assessments and Gait Performance in Patients with Trimalleolar Fractures | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Clinical Assessments and Gait Performance in Patients with Trimalleolar Fractures Ting Zhu, Ya Wang, Fei Tian, Wenjin Wang, Rongzhou Zhong, Hua Zhai, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1494636/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 8 You are reading this latest preprint version Abstract Background: Trimalleolar fracture is a common ankle fracture with serious complications and costly healthcare problem. Most studies used clinical assessments to evaluate the functional status of the patients. Although clinical assessments are valid, they are static and subjective. Dynamic, objective and precise evaluations such as gait analysis are needed. Ankle biomechanics studies on gait in patients with trimalleolar fractures are still rare. This study aimed to investigate the clinical outcomes and gait biomechanics in patients with trimalleolar fractures and compared to healthy controls. Methods: This was a cross-sectional study. 12 patients with trimalleolar fractures were recruited, and 12 healthy people served as controls. All patients underwent clinical assessments: Olerud and Molander ankle score (OMAS), ankle swelling and passive range of motion (ROM) of ankle, and completed gait biomechanical analysis: temporal-spatial parameters, plantar pressure distributions, and surface electromyography (sEMG). The control group only performed gait test. Results: Patients had poor outcomes of clinical assessments. During gait analysis, patients presented compromised gait patterns: shorter step length, larger step width, slower walking speed and shorter single support compared to healthy controls ( p < 0.001), and patients showed asymmetrical gait. Symmetry index of step width and walking speed were mainly correlated with the difference of ankle inversion ROM between two sides ( R =-0.750, P =0.005; R =-0.700, P =0.011). During walking, patients showed abnormal dynamic plantar pressure features (mainly in the hindfoot and forefoot regions), and the IEMG (integrated electromyography) of tibial anterior muscle (TA) and peroneal longus muscle (PL) were larger than healthy controls ( P =0.002, 0.050). Conclusions: Patients with trimalleolar fractures showed physical impairments of the ankle, and presented altered gait parameters compared to healthy subjects. The ankle stability of patients declined, and deficits in TA and PL muscle ability might contribute to it. Restoring complete muscle functions and improving passive ankle ROM are significant to promote the recovery of a normal gait pattern. Ankle trimalleolar fracture Clinical assessments Biomechanics Gait Figures Figure 1 Figure 2 Introduction Ankle fractures are one of the most common lower limb fractures [ 1 ]. Based on the number of malleoli involved, ankle fractures can be classified into isolated malleolar fractures, bimalleolar fractures and trimalleolar fractures, which occur in around 66%, 25% and 7% of all ankle fractures respectively [ 2 ]. The trimalleolar fractures have worse clinical outcomes and an increased incidence of osteoarthrosis [ 2 ], and a notable proportion was unable to return to sports [ 3 ]. Although some studies investigated the differences among subgroups based on the severity of fractures [ 1 , 4 , 5 ], there were few studies focused on the characteristics of trimalleolar fractures alone [ 6 ]. Most studies used radiographic assessment, physical examination and patient-reported outcome measures (PROM) to evaluate the functional status of the patients post ankle fractures surgeries [ 1 , 4 ]. Although these methods are considered to be valid, they are static and subjective, and dynamic, objective and precise evaluations are needed. Gait is the most common and fundamental method for humans to perform physical activities, and the integrated actions of the nervous, muscular and skeletal systems are reflected in walking [ 7 ]. Gait analysis is a significant way of characterizing functional performance of humans, including temporal-spatial parameters (TSPs), gait kinematics, gait kinetics and musculoskeletal activity [ 8 , 9 ]. TSPs are referred to as the vital signs of gait, including walking speed, cadence, step length and so on [ 8 ]. Plantar pressure distribution (PPD) includes many valuable information for evaluating stability and motor control ability of ankle, and it had been estimated in subjects who were at risk of sustaining ankle injuries or ankle instabilities [ 10 ]. The lower limb muscles are the active part of walking, and analyzing muscle activation during functional tasks, such as walking, would be more comprehensively to evaluate the ankle function [ 9 ]. Several studies have investigated lower extremity muscle activation by surface electromyography (sEMG) in patients with chronic ankle instability, ankle sprains and so on [ 7 , 11 ]. Biomechanical studies on gait in patients with trimalleolar fractures are still rare, and to the best of our knowledge, this was the first study to exclusively explore the TSPs, PPD and sEMG characteristics of trimalleolar fractures patients. The primary aim of this study was to investigate the gait biomechanics including TSPs, PPD and sEMG in patients with trimalleolar fractures during walking, and the results were compared with healthy controls. The secondary aim was to evaluate the association between gait parameters and ankle clinical assessments. Methods Participants 12 patients with unilateral trimalleolar fracture postoperatively were recruited as the experimental group and 12 healthy subjects as the control group. The inclusion criteria of the experimental group were as follows: age between 18 ~ 60 years, who were in the recovery period after the fixation of their trimalleolar ankle fractures, and were able to walk independently without the help of aids, and also did not undergo targeted rehabilitation treatment of the ankle joint. Patients were excluded if they 1) isolated malleolar or bimalleolar fractures; 2) open fractures, pathological fractures, multiple injuries; 3) combined with injuries of blood vessels or nerves; or 4) unwilling to sign informed consent. The control group required the subjects to be in good health, aged between 18 ~ 60 years, had no history of ankle joint injury or surgery, had not received ankle joint movement control training in the past year. Exclusion criteria were: 1) any hip, knee and ankle injuries affecting joint activity and diseases of the motor system; 2) abnormal lower limb alignment; 3) acute injury of the musculoskeletal structure of other joints within the previous 3 months; 4) patients with chronic ankle instability; 5) unwilling to sign informed consent. In this study, all healthy subjects were right leg dominant (the preferred leg to kick a soccer) [ 4 ]. All participants had detailed procedures introduced to them before performing experiment and signed the informed consent forms. This study was approved by the Medical Ethics Committee of Shanghai Sunshine Rehabilitation Center. Equipment Walking performance were captured using the eight-cameras Vicon infrared motion capture system (200 Hz) (ViconT40, OxfordMetrics, Oxford, UK). Hemispherical reflective markers were placed on 21 specific anatomic points on the subjects: anterior superior iliac spine, posterior superior iliac spine, middle thigh, medial knee, lateral knee, middle leg, heel, second metatarsal, medial malleolus, lateral malleoluss on both sides of the body and midpoint of upper margin of sacrum. Vicon Nexus was used to process the 3D motions on the computer. The plantar pressure during walking was measured by an Emed ® -x400 plantar pressure system (100 Hz, 700 mm × 400 mm, 4 sensors/cm 2 . ) (Novel GmbH, Munich, Germany). The sEMG signals were collected using a 16-bit Noraxon data acquisition system (1500 Hz) (Noraxon DTS, USA). According to SENIAM international standards, disposable Ag/AgCl circular bipolar electrodes were used (diameter:10 mm; inter-electrode spacing: 2 cm) [ 12 ]. Testing Procedures After inclusion, the basic characteristics were obtained firstly. Then, patients underwent clinical examinations: assessments of the Olerud and Molander Ankle Score (OMAS), ankle circumference, and passive ankle joint mobility. Finally, the biomechanics of gait (temporal-spatial parameters, plantar pressure and sEMG) were tested. Clinical Assessment • OMAS OMAS is a PROM consisting of nine Likert-styled questions for symptom evaluation in patients with an ankle fracture. It was scored 0–100; with higher scores indicating better ankle function [ 13 ]. • Ankle Circumference The circumferences of the ankles were measured with a flexible ruler without elasticity, wrapping around the ankle in a figure of eight [ 14 ]. Ankle swelling was quantified by comparing a patient's injured and noninjured ankle girth difference. • Passive ankle Joint mobility Joint mobility test was conducted based on standard procedures. Range of motion (ROM) in the sagittal plane (dorsiflexion and plantarflexion) and the coronal plane (inversion and eversion) were measured with a manual goniometer. When measuring ROM, the patients were instructed to bring the ankle actively into maximum position, and then the ankle was passively brought to the maximum angle possible by a therapist [ 15 , 16 ]. Gait Analysis All participants underwent gait motion capture by Vicon. Participants were asked to walk barefoot at their natural speed, and six walking trials were recorded. Before the formal testing of plantar pressure, patients were instructed to walk at a self-selected velocity along the walkway for 2 trails to become familiar with the procedures. Then five successful trials in barefoot walking were analysed. When measuring sEMG, electrodes were placed on tibial anterior muscle (TA), peroneal longus (PL), gastrocnemius medial (GM), gastrocnemius lateral (GL) and soleus muscle (S) of both sides. Before testing, subjects performed a 5-minute walking warm-up at a self-selected pace. Participants completed a total of six walking trials, and data were collected for 30 seconds for each trail. Statistical analysis Analyses were performed using SPSS Software (Version 23, Chicago, IL). All data was checked for normality through the Shapiro-Wilk test. Descriptive statistics were used to present the baseline characteristics of participants. Paired-sample t-tests were used to compare the gait analysis of the injured legs (n = 12) with the noninjured ankles (n = 12) of the patients. All gait parameters were compared between the injured legs (n = 12) of patients and with both legs (n = 24) of the healthy controls by 2- tailed, independent t tests. Some studies concluded that the right and left ankle/foot were independent, therefore, some authors suggested consideration of both limbs in the analysis [ 16 , 17 ]. Finally, Pearson’s tests were performed to verify the correlation between the clinical assessments and temporal-spatial parameters of walking. Correlations were classified [direct (+) or inverse (-)] as weak (0–0.3), moderate (0.3–0.5), or strong (0.5–1) [ 16 ]. Differences were considered statistically significant at P values ≤ 0.05. Results Participant characteristics The demographic characteristics and OMAS for the participants were presented in Table 1 . 12 patients and 12 healthy subjects were included in the trimalleolar fractures group and healthy control group respectively. There were no differences in baseline characteristics between the 2 groups. Gait analysis were performed at a mean of 4.50 ± 1.19 (range 3–7) months postoperatively. The mean OMAS score of patients was 56 ± 11.26 (range 35–75). Table 1 Baseline characteristics of participants (Mean ± SD) Trimalleolar fractures (n = 12) Healthy controls (n = 12) P Value Age (years) 42.20 ± 10.20 32.00 ± 13.98 0.070 Height (cm) 164.30 ± 7.45 165.83 ± 8.35 0.658 Weight (kg) 63.90 ± 8.67 61.75 ± 7.18 0.532 BMI (kg/m2) 23.60 ± 2.32 22.41 ± 1.52 0.159 Male/female 7/5 6/6 0.682 Injured/Dominant foot (n): right/left 8/4 12/0 Time from surgery to gait analysis (mo) 4.50 ± 1.19 (3–7) OMAS, mean (range) Pain 21 ± 2.11 (20–25) Stiffness 3 ± 4.83 (0–10) Swelling 2 ± 2.58 (0–5) Stairs climbing 6 ± 2.11 (5–10) Running 0 ± 0 (0–0) Jumping 0 ± 0 (0–0) Squatting 3 ± 2.58 (0–5) Supporting 6.5 ± 4.74 (0–10) Work activity of daily life 14.5 ± 2.84 (10–20) Total score 56 ± 11.26 (35–75) SD: standard deviation; BMI: body mass index; OMAS: the Olerud and Molander Ankle Score Clinical Assessment: Comparison Between The Injured And Noninjured Side Circumference ankle was significantly larger on the injured side compared with the noninjured side in patients ( P < 0.001), and the degree of ankle swelling was 1.41 ± 0.61 cm (Table 2 ). The passive ROM in dorsiflexion, plantar flexion, inversion, and eversion of the injured side were significantly smaller than those of the noninjured side ( P = 0.002, 0.004, < 0.001, 0.004, Table 2 ). Table 2 Clinical assessments of the patients with trimalleolar fractures (Mean ± SD) Injured side (n = 12) Noninjured side (n = 12) P value Difference Circumference ankle (cm) 50.73 ± 3.11 49.32 ± 2.98 <0.001 1.41 ± 0.61 ROM dorsiflexion (degree) 7.08 ± 3.91 13.08 ± 4.10 0.002 6.00 ± 5.13 ROM plantar flexion (degree) 33.33 ± 8.07 41.41 ± 7.83 0.004 8.08 ± 7.77 ROM inversion (degree) 13.75 ± 6.78 26.25 ± 9.64 <0.001 12.50 ± 7.76 ROM eversion (degree) 6.00 ± 4.17 13.25 ± 5.77 0.004 7.25 ± 6.92 SD: standard deviation; ROM: range of motion. The difference of ankle joint circumference is swelling. Swelling = (Circumference of injured side) - (Circumference of noninjured side), and the difference of angle = (ROM of noninjured side) - (ROM of injured side). Gait Analysis: Comparison Between The Injured And Noninjured Side And Between Patients And Healthy Subjects • Temporal-Spatial Parameters The temporal-spatial gait parameters were as shown in Table 3 . The standardized single-support time was expressed by the ratio of the single-support time to the total gait cycle to eliminate individual difference. The symmetry index is calculated using the formula: ((involved-uninvolved)/(involved + uninvolved)/2)⋅100. An absolute value of symmetry index of zero indicates perfect symmetry and up to 5% difference between limbs is considered normal [ 1 ]. Compared with the noninjured side, the step length, walking speed and single support time of the injured side in the patients were significantly decreased ( P = 0.008, 0.041, 0.008, Table 3 ), while the step width was similar between both sides ( P = 0.434). Compared with the healthy subjects, patients demonstrated shorter step length ( P <0.001), wider step width ( P <0.001), slower walking speed ( P <0.001) and less single support time ( P <0.001).The symmetry indexes indicated asymmetry in patients and symmetry in healthy subjects. The symmetry index of the step length and single support time was significant different between the 2 groups ( P = 0.014, 0.028), while the symmetry index of step width and walking speed was similar between the 2 groups ( P = 0.397, 0.052). Table 3 Temporal-spatial gait parameters of the patients with trimalleolar fractures and both sides of the healthy controls Injured side (n = 12) Noninjured side (n = 12) Healthy controls (n = 24) P1 value P2 value Step length (m) 0.48 ± 0.13 0.41 ± 0.15 0.66 ± 0.06 0.008 <0.001 Step width (m) 0.18 ± 0.04 0.18 ± 0.04 0.12 ± 0.03 0.434 <0.001 Walking speed (m/s) 0.65 ± 0.29 0.66 ± 0.29 1.29 ± 0.13 0.041 <0.001 Single support time (%) 16.27 ± 5.63 20.40 ± 2.71 32.27 ± 2.96 0.008 <0.001 Symmetry index step length (%) 21.44 ± 26.34 -0.49 ± 6.02 0.014 Symmetry index step Width (%) -0.48 ± 7.07 -4.65 ± 14.17 0.397 Symmetry index walking speed (%) -3.74 ± 4.01 -0.35 ± 3.67 0.052 Symmetry index single support time (%) -27.63 ± 27.35 -0.94 ± 7.23 0.028 P1: injured side VS noninjured side, P2: injured side VS healthy controls • Dynamic plantar pressure parameters Compared to the noninjured side, the injured side demonstrated lower peak plantar pressure for the T345 (injured side: 61.09 ± 60.13 kpa, noninjured side: 112.27 ± 79.12 kpa, P = 0.022) and smaller contact area for the MF (injured side: 22.60 ± 5.64 cm 2 , noninjured side: 25.87 ± 7.90 cm 2 , P = 0.038). The peak plantar pressure, contact area and contact time in other regions were similar in both sides ( P > 0.05). As shown in Table 4 , compared to the healthy group, the trimalleolar fractures group demonstrated lower peak pressure for the HF, M2, M3, T2, T345 ( P = 0.040, <0.001,<0.001, 0.001, 0.003). The contact areas of the HF, M4, T1, T2, T345 in the trimalleolar fractures group were smaller ( P <0.001, 0.017, 0.004, 0.002,<0.001). The contact time for the HF and MF in the trimalleolar fractures group was increased ( P <0.001, 0.001), and the contact time for the T2, T345 was reduced ( P = 0.001,0.027). The total contact time of the patients and healthy group were (981.00 ± 141.45) ms and (791.14 ± 111.05) ms respectively, P = 0.003. Table 4 Comparison of dynamic plantar pressure features between the injured sides of patients with trimalleolar fractures and both sides of the healthy controls Region Peak plantar pressure (kpa) Contact area (cm 2 ) Contact time (%) Trimalleolar fractures (n = 12) Healthy controls (n = 24) Trimalleolar fractures (n = 12) Healthy controls (n = 24) Trimalleolar fractures (n = 12) Healthy controls (n = 24) HF 290.95 ± 54.45* 358.12 ± 96.90 28.48 ± 3.07* 30.78 ± 3.59 71.06 ± 7.18* 57.33 ± 7.17 MF 127.68 ± 39.75 155.37 ± 47.08 22.60 ± 5.64 25.81 ± 5.32 72.18 ± 7.22* 66.10 ± 5.57 M1 253.00 ± 138.61 277.83 ± 101.60 11.20 ± 2.24 11.93 ± 1.20 79.97 ± 9.04 82.16 ± 3.76 M2 226.22 ± 144.40* 597.62 ± 292.83 9.05 ± 1.23 9.31 ± 0.77 85.32 ± 6.57 84.62 ± 3.61 M3 245.45 ± 147.56* 455.67 ± 149.56 9.97 ± 1.40 10.69 ± 0.92 86.76 ± 6.68 85.55 ± 3.11 M4 192.50 ± 113.27 246.37 ± 39.19 7.97 ± 1.04* 8.75 ± 0.74 83.40 ± 6.60 83.71 ± 3.31 M5 125.22 ± 72.11 184.00 ± 96.74 5.03 ± 0.58 5.43 ± 0.68 76.60 ± 6.32 75.96 ± 4.67 T1 300.18 ± 286.80 417.42 ± 141.87 7.45 ± 2.31* 10.04 ± 0.99 75.26 ± 20.24 73.35 ± 11.52 T2 79.27 ± 81.91* 211.75 ± 104.20 2.25 ± 1.37* 3.92 ± 0.54 43.78 ± 20.71* 65.22 ± 13.57 T345 61.09 ± 60.13* 148.08 ± 78.35 2.81 ± 2.17* 6.84 ± 2.03 42.57 ± 26.22* 63.40 ± 12.06 HF: hindfoot, MF: medial midfoot, M1: the first metatarsal head, M2: the second metatarsal head, M3: the third metatarsal head, M4: the fourth metatarsal head, M5: the fifth metatarsal head, T1: hallux, T1: the second toe, T3-5: the third to fifth toes *P < 0.05 • sEMG The integrated EMG (%) (IEMG) of TA of the injured side (10.84 ± 4.59) in the patients was significantly larger than the noninjured side (8.93 ± 4.56), P = 0.014. While the IEMG (%) of PL (injured side: 29.51 ± 20.43, noninjured side: 54.35 ± 57.27, P = 0.117), GL (injured side: 27.91 ± 17.78, noninjured side: 32.13 ± 19.75, P = 0.581), GM (injured side: 24.78 ± 17.83, noninjured side: 34.44 ± 22.77, P = 0.124) and S (injured side: 21.30 ± 12.65, noninjured side: 21.94 ± 14.41, P = 0.922) were not significant different between both sides. During walking, the IEMG (%) of TA, PL were significantly higher in the patients ( P = 0.002, 0.008) than in the healthy controls, while no differences were identified between groups for IEMG (%) of GL, GM and S (Fig. 1 ). • Correlations analysis The symmetry index of step width and walking speed were highly inversely correlated with the difference of ankle inversion ROM between two sides ( R =-0.750, P = 0.005, Fig. 2 A; R =-0.700, P = 0.011, Fig. 2 B). The symmetry indexes were not correlated with other clinical assessments of the ankle ( P > 0.050). Discussion This study indicated that at an average of 4.5-months post-surgery, patients with trimalleolar fractures showed poor OMAS results, and their injured ankles were swollen, and the passive ROM were decreased. During gait analysis, patients demonstrated abnormal gait compared with healthy controls, and an asymmetrical gait pattern was seen in patients. Compared with healthy controls, the abnormal performance of patients in plantar pressure distribution were concentrated in hindfoot and forefoot, and patients also showed abnormal muscle activity of TA and PL. Furthermore, the passive inversion ROM was highly correlated to symmetry index of step width and walking. This study was the first to indicate remaining detailed gait deficits in patients with trimalleolar fractures. In addition, gait parameters were correlated with clinical outcomes in patients with trimalleolar fractures for the first time. OMAS are usually used as a reliable and valid outcome measure after an ankle fracture[ 18 ], and based on the total score, ankle function of patients could be divided into four grades: excellent (OMAS:100 to 91 points), good (OMAS:90 to 61 points), fair (OMAS:60 to 31 points) and poor (OMAS:30 to 0 points). [ 19 ] In this study, the mean OMAS was 56, indicating that patients with trimalleolar fractures reported fair ankle function. According to the study, the disability of running and jumping contributed most to the total score. Several studies also investigated the OMAS of ankle fractures patients, and they showed better results than our study. Oguzhan Tano glu et al. [ 20 ]compared the effect of a 1-stage surgery for the unstable malleolar fracture dislocations with the 2-stage surgery. The two group all included patients with isolated malleolar fractures, bimalleolar fractures and trimalleolar fractures. And the duration of follow-up of the two group was 21.7 and 19.2 months respectively. The mean OMAS was 87.8 for the 1-stage surgery group and 83.2 for the 2-stage surgery. Mareen Braunstein et al. [ 21 ]demonstrated functional outcomes after 1 year of arthroscopically assisted ankle fracture treatment, and they reported a mean OMAS of 85 for trimalleolar fractures. The poor OMAS results for trimalleolar fractures in this study might be mainly attributed to the short length of the postoperative follow-up period. This study investigated the clinical outcomes after 4.5 months postoperatively, while other studies evaluated the long-term (more than 1 year) clinical outcomes. This study indicated that patients with trimalleolar fractures remained physical impairments postoperatively. Compared with the noninjured side, patients represented ankle swelling, and a decrease in passive ROM on the injured side. Ankle swelling is a common and long-standing complication after surgery. It was reported that more than half of the patients following unimalleolar and bimalleolar ankle fractures presented stiffness, swelling and pain [ 1 ]. 60% or more of the patients 65 years or older reported ankle pain, swelling and problems when using stairs and reduced activities of daily life one year after ankle fractures [ 22 ]. Shah et al.[ 23 ]demonstrated that around 45% of 69 patients with Weber B and C ankle fractures still had ankle swelling at 5 years after the injury. Our study investigated the passive ROM, and mean (sd) angle of dorsiflexion, plantarflexion, inversion and eversion of the injured leg on the trimalleolar fractures patients was 7.08 (3.91) degrees, 33.33 (8.07) degrees, 13.75 (6.78) degrees and 6.00 (4.17) degrees respectively. Ganit Segal et al. [ 1 ] also measured the passive ankle ROM of patients with trimalleolar fractures in the sagittal (dorsiflexion/ plantar flexion) and coronal plane (inversion/eversion), and the ROM was − 0.8 (7.6) degrees, 40.6 (7.5) degrees, 5.6 (3.6) degrees and 2.5 (4.0) degrees respectively. The result was a little different from our study, might due to the assessment point. Ganit Segal et al. [ 1 ] measured ROM at 64.5 days from injury, which was earlier than ours (4.5 months). There was also a study evaluated ROM during activities. van Hoeve, S et al. [ 4 ] found that compared with the healthy subjects (12.59 ̊ ± 3.73 ̊), the ROM during gait in patients (7.13 ̊ ± 2.55 ̊) with trimalleolar ankle fractures decreased significantly. After ankle fractures, the uncoagulated hemorrhage leads to the rapid increase of intra-articular pressure, which causes abrupt joint swelling, pain and limited mobility [24]. Presence of soft tissue damages such as tendon and ligamental injuries can cause chronic swelling and stiffness therefore resulting in the dismal outcome [3]. These complications might alter the gait. The present study showed that patients with trimalleolar fractures presented compromised gait pattern. The temporal-spatial parameters of the injured side were significantly different from the noninjured side and healthy subjects, except no difference exist in step width between both sides. This is in line with other research findings. Two studies investigated the gait parameters of patients with trimalleolar fractures, and compared those with healthy group. They found all gait parameters were significantly below the normal range[ 1 , 6 ]. Andrew F. Tyler et al. [ 6 ] also showed that the gait characteristics of ankle fracture patients were more similar to healthy elderly patients. However, these two studies only investigated the differences between patients and healthy subjects, but did not compare the gait parameters of the injured side with the noninjured side. Ganit Segal et al. [ 1 ]also examined limb symmetry of the gait patterns, and found significant asymmetry in step length and single limb support. These findings were consistent with this study. And by correlation analysis in this study, step asymmetry might be related to the difference of ankle inversion ROM between two sides. All these results presented that patients with trimalleolar fractures did not achieve restoration of normal physiologic gait in the short-term, and patients adopted a simple security strategy with a reduction of walking speed[ 7 ]. The differences of features of plantar pressure distributions and sEMG in both sides also indicated asymmetries in gait in patients with trimalleolar fractures. The plantar pressure in the T345 and the contact area of MF of the injured side were significantly smaller than those of the noninjured side, and this was probably due to a more cautious and compensatory walking pattern, by further biasing the center of gravity to the noninjured side. Sjoerd Kolk et al.[ 25 ] also showed subtle asymmetries in gait kinetics and kinematics between the operated and non-operated limbs, and they considered that patients performed a more cautious walking pattern and an integral strategy. Plantar pressures of other type ankle fractures such as pilon fractures, calcaneal fractures were also asymmetry, and adhesion or conduction disorders at the tibia may be causes of abnormal plantar pressure [ 25 , 26 ]. Compared with normal healthy subjects, patients with trimalleolar fracture performed abnormal gait during walking support period. Patients tended to step more cautiously on the injured heel, showing smaller peak plantar pressure in HF, and this might be due to pain or psychological factors such as fear or worry of reinjury [ 27 ]. For patients, the contact time (%) of HF and MF and total contact time were significantly increased, and it might be associated with lower ankle stability: for patients with trimalleolar fractures, the lateral, medial and posterior malleolus were injured, probably impairing the ankle stability, and patients needed more time to maintain ankle stability [ 1 ]. The muscle activity on the TA and PL of the injured side was significantly larger than those in the noninjured, also indicating the ankle stability of the injured side decreased, because muscular co-contraction of TA and PL was increased to stabilize the ankle joint [ 28 ]. And in this study, the step width was significantly smaller than that of healthy controls, also showing the walk stability decreased in patients. The plantar pressure features (smaller peak plantar pressure and contact area, shorter contact time) in the forefoot demonstrated that the propulsion ability during walking significantly decreased in patient, and this could decrease the walking speed. In this study, the IEMG of PL of patients was significantly increased, indicating the muscle ability decreased. Therefore, it was reasonable to speculate that the abnormal gait features in the forefoot might be related to the decreased ability of PL. During normal walking, the plantar pressure in the first metatarsal head plays a key role in pushing off [ 29 ]. And the PL is essential for maintaining ankle stability and plays an important role in the push-off stage. The PL origins at the proximal tibia and fibula, and inserts at the first metatarsal and medial cuneiform [ 30 ]. Except for contributing to 63% of eversion strength, the PL is helpful to initiate pronation and stabilize first ray during propulsion phases of gait [ 30 ]. Limitations There were some limitations in this study. First, this study did not divide the patients into “unfixed posterior malleoli group” and “fixed Posterior malleoli group”. However, it was demonstrated that fixation of the posterior malleolus particularly did not appear to improve gait characteristics. Therefore, the patients in this study were not further grouped into two subgroups. Second, the participants in this study were asked to walk at their natural speed, and these values in healthy subjects were found to be faster when compared to the patients, which may affect the comparisons between the other gait biomechanics parameters. Future research should examine the difference in gait patterns when patients walk at the same speed. Third, this was a cross-sectional evaluation of patients following ankle fracture. Future research should investigate long-term differences in clinical outcomes and gait patterns, to evaluate the function of patients with trimalleolar fractures comprehensively. Conclusion Patients with trimalleolar fractures showed poor PROM results, ankle swelling and smaller passive ROM. Compared to healthy controls, patients showed altered temporal-spatial, plantar pressure and sEMG parameters. Gait asymmetries were correlated to the difference of ankle inversion ROM between two sides. The ankle stability of patients declined, and deficits in TA and PL muscle ability might contribute to it. The propulsion ability of patients during walking significantly decreased, and this was mainly correlated to the deficit in PL ability. Restoring complete muscle functions and improving passive ankle ROM are significant to guarantee the recovery of a normal gait pattern, which is helpful to the recovery of normal activities of daily life and sports. Declarations Ethics approval and consent to participate This study was performed in accordance with the Declaration of Helsinki. This study was approved by the Medical Ethics Committee of Shanghai Sunshine Rehabilitation Center (No: YZ2019-001). Before the experiment started, all participants had detailed procedures introduced and signed the informed consent documents. The written informed consent was obtained. Consent for publication Not applicable. Availability of data and materials All data generated or analysed during this study are included in this published article. Competing interests The authors do not have any conflicts of interest to disclose. Funding This work was supported by the National Key Research and Development Program of China [grant number 2018YFF0300504]; Science and Technology Commission of Shanghai Municipality [grant number 18441907200]. Authors' contributions (I) Conception and design: Ting Zhu, Shaobai Wang; (II) Provision of study materials or patients: Rongzhou Zhong, Hua Zhai;(III) Collection and assembly of data: Ting Zhu, Ya Wang, Wenjin Wang; (IV) Data analysis and interpretation: Ting Zhu, Ya Wang, Fei Tian; (V) Manuscript writing: Ting Zhu; (VI) Final approval of manuscript: Hua Zhai, Shaobai Wang. Acknowledgements Thanks to all the subjects who participated in this study. We would like to acknowledge Miss Xia Ma and Mrs. Yujuan Zhang for their assistance with recruiting subjects. References G. Segal, A. Elbaz, A. Parsi, Z. Heller, E. Palmanovich, M. Nyska, et al., Clinical outcomes following ankle fracture: a cross-sectional observational study, Journal of foot and ankle research. 2014;7(1): 50. G. Testa, M. Ganci, M. Amico, G. Papotto, S.M.C. Giardina, G. Sessa, et al., Negative prognostic factors in surgical treatment for trimalleolar fractures, European journal of orthopaedic surgery & traumatology: orthopedie traumatology. 2019;29(6): 1325–1330. C.C. Hong, N. Nashi, S. Prosad Roy, K.J. Tan, Impact of trimalleolar ankle fractures: how do patients fare post-operatively?, Foot and ankle surgery: official journal of the European Society of Foot and Ankle Surgeons. 2014;20(1): 48–51. S. van Hoeve, M. Houben, J. Verbruggen, P. Willems, K. Meijer, M. Poeze, Gait analysis related to functional outcome in patients operated for ankle fractures, Journal of orthopaedic research: official publication of the Orthopaedic Research Society. 2019;37(7): 1658–1666. R. Wang, C.K. Thur, E.M. Gutierrez-Farewik, P. Wretenberg, E. Broström, One year follow-up after operative ankle fractures: a prospective gait analysis study with a multi-segment foot model, Gait & posture. 2010;31(2): 234–40. A. Tyler, T. Rose, S. Day, J. Kenia, A. Horan, S. Mehta, et al., Comparison of Spatiotemporal Gait Parameters Following Operative Treatment of Trimalleolar Ankle Fractures vs Healthy Controls, Foot & Ankle Orthopaedics. 2020;5: 247301142093105. I.M. Punt, J.-L. Ziltener, M. Laidet, S. Armand, L. Allet, Gait and Physical Impairments in Patients With Acute Ankle Sprains Who Did Not Receive Physical Therapy, PM&R 2015;7(1): 34–41. J.M. Kane, S. Coleman, J.W. Brodsky, Kinematics and Function of Total Ankle Replacements Versus Normal Ankles, Foot and ankle clinics. 2017;22(2): 241–249. S. Blair, M.J. Lake, R. Ding, T. Sterzing, Magnitude and variability of gait characteristics when walking on an irregular surface at different speeds, Human movement science. 2018;59: 112–120. A.K. Buldt, S. Forghany, K.B. Landorf, P. Levinger, G.S. Murley, H.B. Menz, Foot posture is associated with plantar pressure during gait: A comparison of normal, planus and cavus feet, Gait & posture. 2018;62: 235–240. M.A. Feger, L. Donovan, J.M. Hart, J. Hertel, Lower Extremity Muscle Activation in Patients With or Without Chronic Ankle Instability During Walking, Journal of Athletic Training. 2015;50(4): 350–357. I. Campanini, C. Disselhorst-Klug, W.Z. Rymer, R. Merletti, Surface EMG in Clinical Assessment and Neurorehabilitation: Barriers Limiting Its Use, Frontiers in neurology. 2020;11: 934. C. Olerud, H. Molander, A scoring scale for symptom evaluation after ankle fracture, Archives of orthopaedic and traumatic surgery. Archiv fur orthopadische und Unfall-Chirurgie. 1984;103(3): 190–4. M.L. Pugia, C.J. Middel, S.W. Seward, J.L. Pollock, R.C. Hall, L. Lowe, et al., Comparison of acute swelling and function in subjects with lateral ankle injury, The Journal of orthopaedic and sports physical therapy. 2001;31(7): 384–8. R.A. Elveru, J.M. Rothstein, R.L. Lamb, Goniometric reliability in a clinical setting. Subtalar and ankle joint measurements, Physical therapy. 1988;68(5): 672–7. I.M. Punt, J.L. Ziltener, M. Laidet, S. Armand, L. Allet, Gait and physical impairments in patients with acute ankle sprains who did not receive physical therapy, PM & R: the journal of injury, function, and rehabilitation. 2015;7(1): 34–41. P. Aleixo, J. Vaz Patto, A. Cardoso, H. Moreira, J. Abrantes, Ankle kinematics and kinetics during gait in healthy and rheumatoid arthritis post-menopausal women, Somatosensory & motor research. 2019;36(2): 171–178. A.M. Garratt, M.G. Naumann, U. Sigurdsen, S.E. Utvåg, K. Stavem, Evaluation of three patient reported outcome measures following operative fixation of closed ankle fractures, BMC musculoskeletal disorders. 2018;19(1): 134. S. Görtz, A.J. De Young, W.D. Bugbee, Fresh osteochondral allografting for osteochondral lesions of the talus, Foot & ankle international. 2010;31(4): 283–90. O. Tanoğlu, M.B. Gökgöz, A. Özmeriç, K.B. Alemdaroğlu, Two-Stage Surgery for the Malleolar Fracture-Dislocation With Severe Soft Tissue Injuries Does Not Affect the Functional Results, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2019;58(4): 702–705. M. Braunstein, S.F. Baumbach, M. Urresti-Gundlach, L. Borgmann, W. Böcker, H. Polzer, Arthroscopically Assisted Treatment of Complex Ankle Fractures: Intra-articular Findings and 1-Year Follow-Up, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2020;59(1): 9–15. G. Nilsson, K. Jonsson, C. Ekdahl, M. Eneroth, Outcome and quality of life after surgically treated ankle fractures in patients 65 years or older, BMC musculoskeletal disorders. 2007;8: 127. N.H. Shah, R.O. Sundaram, A. Velusamy, I.J. Braithwaite, Five-year functional outcome analysis of ankle fracture fixation, Injury. 2007;38(11): 1308–1312. C. Liu, J. Guo, Q. Cui, D. Li, Y. Zeng, Clinical and imaging analysis of subclinical hemophilia combined with coxarthrosis: case report and literature review, Springerplus. 2016;5(1): 2058–2058. S. Kolk, R. Fluit, J. Luijten, P.J. Heesterbeek, A.C. Geurts, N. Verdonschot, et al., Gait and lower limb muscle strength in women after triple innominate osteotomy, BMC musculoskeletal disorders. 2015;16: 68. S. van Hoeve, J. de Vos, J.P. Verbruggen, P. Willems, K. Meijer, M. Poeze, Gait Analysis and Functional Outcome After Calcaneal Fracture, The Journal of bone and joint surgery. American volume. 2015;97(22): 1879–88. S. Jandova, J. Pazour, M. Janura, Comparison of Plantar Pressure Distribution During Walking After Two Different Surgical Treatments for Calcaneal Fracture, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2019;58(2): 260–265. K. Sasaki, R.R. Neptune, Differences in muscle function during walking and running at the same speed, Journal of biomechanics. 2006;39(11): 2005–13. F. Pu, W. Ren, H. Fu, X. Zheng, M. Yang, Y.K. Jan, et al., Plantar blood flow response to accumulated pressure stimulus in diabetic people with different peak plantar pressure: a non-randomized clinical trial, Medical & biological engineering & computing. 2018;56(7): 1127–1134. D. Koh, L. Liow, J. Cheah, K. Koo, Peroneus longus tendon rupture: A case report, World journal of orthopedics. 2019;10(1): 45–53. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Major revision 10 May, 2022 Reviews received at journal 04 May, 2022 Reviewers agreed at journal 22 Apr, 2022 Reviewers invited by journal 22 Apr, 2022 Editor assigned by journal 07 Apr, 2022 Editor invited by journal 30 Mar, 2022 Submission checks completed at journal 30 Mar, 2022 First submitted to journal 27 Mar, 2022 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1494636","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":94856845,"identity":"bcc16a56-04df-45b5-9c91-713178df27c4","order_by":0,"name":"Ting Zhu","email":"","orcid":"","institution":"Shanghai University of Sport","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ting","middleName":"","lastName":"Zhu","suffix":""},{"id":94856846,"identity":"29c81b41-cd1f-4fa1-8ccb-2fba8a25311c","order_by":1,"name":"Ya Wang","email":"","orcid":"","institution":"Yangzhi Affiliated Rehabilitation Hospital of Tongji University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ya","middleName":"","lastName":"Wang","suffix":""},{"id":94856847,"identity":"57e26373-ed09-4b0e-9790-3d3b75233c11","order_by":2,"name":"Fei Tian","email":"","orcid":"","institution":"Shanghai University of Sport","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Fei","middleName":"","lastName":"Tian","suffix":""},{"id":94856848,"identity":"19ae83bc-947f-4887-a971-07aefcf5ca91","order_by":3,"name":"Wenjin Wang","email":"","orcid":"","institution":"Shanghai University of Sport","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Wenjin","middleName":"","lastName":"Wang","suffix":""},{"id":94856849,"identity":"6069f229-2108-4934-872b-340e5bb37d8b","order_by":4,"name":"Rongzhou Zhong","email":"","orcid":"","institution":"Yangzhi Affiliated Rehabilitation Hospital of Tongji University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Rongzhou","middleName":"","lastName":"Zhong","suffix":""},{"id":94856850,"identity":"fbf9b705-fd2c-415e-b74c-8ba83bd1c562","order_by":5,"name":"Hua Zhai","email":"","orcid":"","institution":"Yangzhi Affiliated Rehabilitation Hospital of Tongji University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Hua","middleName":"","lastName":"Zhai","suffix":""},{"id":94856851,"identity":"904473db-65ed-4242-b0d9-3dd5b4caeb81","order_by":6,"name":"Shaobai Wang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA2klEQVRIiWNgGAWjYNCCCgkowwCIJRgbiNByBqrlANFaGNugjAMgQgK3QohLjp89/PLnPIs8c/buxMcfCmzyGKSbmz/g1XImL82ad5tEsWXP2c0GBwzSihlkDjYY4NNidiDHzJhxm0Tihhu52yQOGBxObJBIbEjAq+X8GzPDn3PQtBzAq+VGjvED3gZULY0N+LTY33hjxsxzDKjlDNAvZwzSEttkDjbj08Eg2Z9j/PFHTV3ihuO9Gx9U/LFJ7Jduf4w3xICADTUi2AgoBwFmQmaOglEwCkbBSAcA10NSTDnzadYAAAAASUVORK5CYII=","orcid":"","institution":"Shanghai University of Sport","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Shaobai","middleName":"","lastName":"Wang","suffix":""}],"badges":[],"createdAt":"2022-03-27 15:29:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1494636/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1494636/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":19876341,"identity":"42aafb6f-5444-4879-83f3-066900af0359","added_by":"auto","created_at":"2022-04-01 20:18:56","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":9047,"visible":true,"origin":"","legend":"\u003cp\u003eComparison of electromyography characteristics between the injured sides of patients with trimalleolar fractures and both sides of the healthy controls\u003c/p\u003e\u003cp\u003eTA: tibial anterior muscle, PL: peroneal longus, GM: gastrocnemius medial, GL: gastrocnemius lateral, S: soleus muscle\u003c/p\u003e\u003cp\u003eIEMG: integrated electromyography\u003c/p\u003e\u003cp\u003e** P \u0026lt; 0.05\u003c/p\u003e\u003cp\u003ens: no significant difference\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-1494636/v1/a3054c0f9400ff357e3df237.png"},{"id":19876342,"identity":"ac7a62cc-581c-49e8-884a-a28c0fb6e467","added_by":"auto","created_at":"2022-04-01 20:18:56","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":57147,"visible":true,"origin":"","legend":"\u003cp\u003eCorrelations between the symmetry index((A) step width, (B) walking speed)\u003c/p\u003e\u003cp\u003eand the difference of ankle inversion ROM\u003c/p\u003e\u003cp\u003eROM: range of motion\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-1494636/v1/57f18c0f2696417663e7a87e.png"},{"id":19876343,"identity":"5221405b-040e-4bcd-8638-635e2d5c884b","added_by":"auto","created_at":"2022-04-01 20:19:00","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":544539,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1494636/v1/8e0f8cfe-3e6f-4ba4-8dbc-7acd54e67b33.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Clinical Assessments and Gait Performance in Patients with Trimalleolar Fractures","fulltext":[{"header":"Introduction","content":"\u003cp\u003eAnkle fractures are one of the most common lower limb fractures [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. Based on the number of malleoli involved, ankle fractures can be classified into isolated malleolar fractures, bimalleolar fractures and trimalleolar fractures, which occur in around 66%, 25% and 7% of all ankle fractures respectively [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. The trimalleolar fractures have worse clinical outcomes and an increased incidence of osteoarthrosis [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e], and a notable proportion was unable to return to sports [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eAlthough some studies investigated the differences among subgroups based on the severity of fractures [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e, \u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e], there were few studies focused on the characteristics of trimalleolar fractures alone [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. Most studies used radiographic assessment, physical examination and patient-reported outcome measures (PROM) to \u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003eevaluate\u003c/span\u003e the functional status of the patients post ankle fractures surgeries [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]. Although these methods are \u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003econsidered to be\u003c/span\u003e valid, they are static and subjective, and dynamic, objective and precise evaluations are needed.\u003c/p\u003e \u003cp\u003eGait is the most common and fundamental method for humans to perform physical activities, and the integrated actions of the nervous, muscular and skeletal systems are reflected in walking [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Gait analysis is a significant way of characterizing functional performance of humans, including temporal-spatial parameters (TSPs), gait kinematics, gait kinetics and musculoskeletal activity [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e, \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. TSPs are referred to as the vital signs of gait, including walking speed, cadence, step length and so on [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e]. Plantar pressure distribution (PPD) includes many valuable information for evaluating stability and motor control ability of ankle, and it had been estimated in subjects who were at risk of sustaining ankle injuries or ankle instabilities [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. The lower limb muscles are the active part of walking, and analyzing muscle activation during functional tasks, such as walking, would be more comprehensively to evaluate the ankle function [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. Several studies have investigated lower extremity muscle activation by surface electromyography (sEMG) in patients with chronic ankle instability, ankle sprains and so on [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e, \u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. Biomechanical studies on gait in patients with trimalleolar fractures are still rare, and to the best of our knowledge, this was the first study to exclusively explore the TSPs, PPD and sEMG characteristics of trimalleolar fractures patients.\u003c/p\u003e \u003cp\u003eThe primary aim of this study was to investigate the gait biomechanics including TSPs, PPD and sEMG in patients with trimalleolar fractures during walking, and the results were compared with healthy controls. The secondary aim was to evaluate the association between gait parameters and ankle clinical assessments.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\n\u003ch2\u003eParticipants\u003c/h2\u003e\n\u003cp\u003e12 patients with unilateral trimalleolar fracture postoperatively were recruited as the experimental group and 12 healthy subjects as the control group. The inclusion criteria of the experimental group were as follows: age between 18\u0026thinsp;~\u0026thinsp;60 years, who were in the recovery period after the fixation of their trimalleolar ankle fractures, and were able to walk independently without the help of aids, and also did not undergo targeted rehabilitation treatment of the ankle joint. Patients were excluded if they 1) isolated malleolar or bimalleolar fractures; 2) open fractures, pathological fractures, multiple injuries; 3) combined with injuries of blood vessels or nerves; or 4) unwilling to sign informed consent. The control group required the subjects to be in good health, aged between 18\u0026thinsp;~\u0026thinsp;60 years, had no history of ankle joint injury or surgery, had not received ankle joint movement control training in the past year. Exclusion criteria were: 1) any hip, knee and ankle injuries affecting joint activity and diseases of the motor system; 2) abnormal lower limb alignment; 3) acute injury of the musculoskeletal structure of other joints within the previous 3 months; 4) patients with chronic ankle instability; 5) unwilling to sign informed consent. In this study, all healthy subjects were right leg dominant (the preferred leg to kick a soccer) [\u003cspan class=\"CitationRef\"\u003e4\u003c/span\u003e]. All participants had detailed procedures introduced to them before performing experiment and signed the informed consent forms. This study was approved by the Medical Ethics Committee of Shanghai Sunshine Rehabilitation Center.\u003c/p\u003e\n\u003c/div\u003e\n\u003ch2\u003eEquipment\u003c/h2\u003e\n\u003cp\u003eWalking performance were captured using the eight-cameras Vicon infrared motion capture system (200 Hz) (ViconT40, OxfordMetrics, Oxford, UK). Hemispherical reflective markers were placed on 21 specific anatomic points on the subjects: anterior superior iliac spine, posterior superior iliac spine, middle thigh, medial knee, lateral knee, middle leg, heel, second metatarsal, medial malleolus, lateral malleoluss on both sides of the body and midpoint of upper margin of sacrum. Vicon Nexus was used to process the 3D motions on the computer. The plantar pressure during walking was measured by an Emed \u0026reg; -x400 plantar pressure system (100 Hz, 700 mm \u0026times; 400 mm, 4 sensors/cm\u003csup\u003e2\u003c/sup\u003e. ) (Novel GmbH, Munich, Germany). The sEMG signals were collected using a 16-bit Noraxon data acquisition system (1500 Hz) (Noraxon DTS, USA). According to SENIAM international standards, disposable Ag/AgCl circular bipolar electrodes were used (diameter:10 mm; inter-electrode spacing: 2 cm) [\u003cspan class=\"CitationRef\"\u003e12\u003c/span\u003e].\u003c/p\u003e\n\u003ch2\u003eTesting Procedures\u003c/h2\u003e\n\u003cp\u003eAfter inclusion, the basic characteristics were obtained firstly. Then, patients underwent clinical examinations: assessments of the Olerud and Molander Ankle Score (OMAS), ankle circumference, and passive ankle joint mobility. Finally, the biomechanics of gait (temporal-spatial parameters, plantar pressure and sEMG) were tested.\u003c/p\u003e\n\u003ch2\u003eClinical Assessment\u003c/h2\u003e\n\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e\n\u003ch2\u003e• OMAS\u003c/h2\u003e\n\u003cp\u003eOMAS is a PROM consisting of nine Likert-styled questions for symptom evaluation in patients with an ankle fracture. It was scored 0\u0026ndash;100; with higher scores indicating better ankle function [\u003cspan class=\"CitationRef\"\u003e13\u003c/span\u003e].\u003c/p\u003e\n\u003cdiv id=\"Sec8\" class=\"Section3\"\u003e\n\u003ch2\u003e• Ankle Circumference\u003c/h2\u003e\n\u003cp\u003eThe circumferences of the ankles were measured with a flexible ruler without elasticity, wrapping around the ankle in a figure of eight [\u003cspan class=\"CitationRef\"\u003e14\u003c/span\u003e]. Ankle swelling was quantified by comparing a patient's injured and noninjured ankle girth difference.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec9\" class=\"Section3\"\u003e\n\u003ch2\u003e• Passive ankle Joint mobility\u003c/h2\u003e\n\u003cp\u003eJoint mobility test was conducted based on standard procedures. Range of motion (ROM) in the sagittal plane (dorsiflexion and plantarflexion) and the coronal plane (inversion and eversion) were measured with a manual goniometer. When measuring ROM, the patients were instructed to bring the ankle actively into maximum position, and then the ankle was passively brought to the maximum angle possible by a therapist [\u003cspan class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e16\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003c/div\u003e\n\u003ch2\u003eGait Analysis\u003c/h2\u003e\n\u003cp\u003eAll participants underwent gait motion capture by Vicon. Participants were asked to walk barefoot at their natural speed, and six walking trials were recorded. Before the formal testing of plantar pressure, patients were instructed to walk at a self-selected velocity along the walkway for 2 trails to become familiar with the procedures. Then five successful trials in barefoot walking were analysed. When measuring sEMG, electrodes were placed on tibial anterior muscle (TA), peroneal longus (PL), gastrocnemius medial (GM), gastrocnemius lateral (GL) and soleus muscle (S) of both sides. Before testing, subjects performed a 5-minute walking warm-up at a self-selected pace. Participants completed a total of six walking trials, and data were collected for 30 seconds for each trail.\u003c/p\u003e\n\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\n\u003ch2\u003eStatistical analysis\u003c/h2\u003e\n\u003cp\u003eAnalyses were performed using SPSS Software (Version 23, Chicago, IL). All data was checked for normality through the Shapiro-Wilk test. Descriptive statistics were used to present the baseline characteristics of participants. Paired-sample t-tests were used to compare the gait analysis of the injured legs (n\u0026thinsp;=\u0026thinsp;12) with the noninjured ankles (n\u0026thinsp;=\u0026thinsp;12) of the patients. All gait parameters were compared between the injured legs (n\u0026thinsp;=\u0026thinsp;12) of patients and with both legs (n\u0026thinsp;=\u0026thinsp;24) of the healthy controls by 2- tailed, independent t tests. Some studies concluded that the right and left ankle/foot were independent, therefore, some authors suggested consideration of both limbs in the analysis [\u003cspan class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e17\u003c/span\u003e]. Finally, Pearson\u0026rsquo;s tests were performed to verify the correlation between the clinical assessments and temporal-spatial parameters of walking. Correlations were classified [direct (+) or inverse (-)] as weak (0\u0026ndash;0.3), moderate (0.3\u0026ndash;0.5), or strong (0.5\u0026ndash;1) [\u003cspan class=\"CitationRef\"\u003e16\u003c/span\u003e]. Differences were considered statistically significant at \u003cem\u003eP\u003c/em\u003e values\u0026thinsp;\u0026le;\u0026thinsp;0.05.\u003c/p\u003e\n\u003c/div\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\n\u003ch2\u003eParticipant characteristics\u003c/h2\u003e\n\u003cp\u003eThe demographic characteristics and OMAS for the participants were presented in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e. 12 patients and 12 healthy subjects were included in the trimalleolar fractures group and healthy control group respectively. There were no differences in baseline characteristics between the 2 groups. Gait analysis were performed at a mean of 4.50\u0026thinsp;\u0026plusmn;\u0026thinsp;1.19 (range 3\u0026ndash;7) months postoperatively. The mean OMAS score of patients was 56\u0026thinsp;\u0026plusmn;\u0026thinsp;11.26 (range 35\u0026ndash;75).\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003ctable id=\"Tab1\" border=\"1\"\u003e\u003ccaption\u003e\n\u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n\u003cdiv class=\"CaptionContent\"\u003e\n\u003cp\u003eBaseline characteristics of participants (Mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD)\u003c/p\u003e\n\u003c/div\u003e\n\u003c/caption\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eTrimalleolar fractures (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eHealthy controls (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e\u003cem\u003eP\u003c/em\u003e Value\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"1\" align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n\u003c/tr\u003e\n\u003c/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eAge (years)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e42.20\u0026thinsp;\u0026plusmn;\u0026thinsp;10.20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e32.00\u0026thinsp;\u0026plusmn;\u0026thinsp;13.98\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e0.070\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eHeight (cm)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e164.30\u0026thinsp;\u0026plusmn;\u0026thinsp;7.45\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e165.83\u0026thinsp;\u0026plusmn;\u0026thinsp;8.35\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e0.658\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eWeight (kg)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e63.90\u0026thinsp;\u0026plusmn;\u0026thinsp;8.67\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e61.75\u0026thinsp;\u0026plusmn;\u0026thinsp;7.18\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e0.532\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eBMI (kg/m2)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e23.60\u0026thinsp;\u0026plusmn;\u0026thinsp;2.32\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e22.41\u0026thinsp;\u0026plusmn;\u0026thinsp;1.52\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e0.159\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eMale/female\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e7/5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e6/6\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e0.682\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eInjured/Dominant foot (n): right/left\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e8/4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e12/0\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eTime from surgery to gait analysis (mo)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e4.50\u0026thinsp;\u0026plusmn;\u0026thinsp;1.19 (3\u0026ndash;7)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"4\" rowspan=\"6\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eOMAS, mean (range)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003ePain\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e21\u0026thinsp;\u0026plusmn;\u0026thinsp;2.11 (20\u0026ndash;25)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eStiffness\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e3\u0026thinsp;\u0026plusmn;\u0026thinsp;4.83 (0\u0026ndash;10)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSwelling\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e2\u0026thinsp;\u0026plusmn;\u0026thinsp;2.58 (0\u0026ndash;5)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eStairs climbing\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e6\u0026thinsp;\u0026plusmn;\u0026thinsp;2.11 (5\u0026ndash;10)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eRunning\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0\u0026thinsp;\u0026plusmn;\u0026thinsp;0 (0\u0026ndash;0)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"4\" rowspan=\"6\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eJumping\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0\u0026thinsp;\u0026plusmn;\u0026thinsp;0 (0\u0026ndash;0)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSquatting\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e3\u0026thinsp;\u0026plusmn;\u0026thinsp;2.58 (0\u0026ndash;5)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSupporting\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e6.5\u0026thinsp;\u0026plusmn;\u0026thinsp;4.74 (0\u0026ndash;10)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eWork activity of daily life\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e14.5\u0026thinsp;\u0026plusmn;\u0026thinsp;2.84 (10\u0026ndash;20)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eTotal score\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e56\u0026thinsp;\u0026plusmn;\u0026thinsp;11.26 (35\u0026ndash;75)\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"6\"\u003eSD: standard deviation; BMI: body mass index; OMAS: the Olerud and Molander Ankle Score\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tfoot\u003e\n\u003c/table\u003e\n\u003c/div\u003e\n\u003c/div\u003e\n\u003ch2\u003eClinical Assessment: Comparison Between The Injured And Noninjured Side\u003c/h2\u003e\n\u003cp\u003eCircumference ankle was significantly larger on the injured side compared with the noninjured side in patients (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.001), and the degree of ankle swelling was 1.41\u0026thinsp;\u0026plusmn;\u0026thinsp;0.61 cm (Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e). The passive ROM in dorsiflexion, plantar flexion, inversion, and eversion of the injured side were significantly smaller than those of the noninjured side (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.002, 0.004, \u0026lt; 0.001, 0.004, Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003ctable id=\"Tab2\" border=\"1\"\u003e\u003ccaption\u003e\n\u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\n\u003cdiv class=\"CaptionContent\"\u003e\n\u003cp\u003eClinical assessments of the patients with trimalleolar fractures (Mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD)\u003c/p\u003e\n\u003c/div\u003e\n\u003c/caption\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eInjured side\u003c/p\u003e\n\u003cp\u003e(n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eNoninjured side (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003e\u003cem\u003eP\u003c/em\u003e value\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eDifference\u003c/p\u003e\n\u003c/th\u003e\n\u003c/tr\u003e\n\u003c/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eCircumference ankle (cm)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e50.73\u0026thinsp;\u0026plusmn;\u0026thinsp;3.11\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e49.32\u0026thinsp;\u0026plusmn;\u0026thinsp;2.98\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e1.41\u0026thinsp;\u0026plusmn;\u0026thinsp;0.61\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eROM dorsiflexion (degree)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e7.08\u0026thinsp;\u0026plusmn;\u0026thinsp;3.91\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e13.08\u0026thinsp;\u0026plusmn;\u0026thinsp;4.10\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.002\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e6.00\u0026thinsp;\u0026plusmn;\u0026thinsp;5.13\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eROM plantar flexion (degree)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e33.33\u0026thinsp;\u0026plusmn;\u0026thinsp;8.07\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e41.41\u0026thinsp;\u0026plusmn;\u0026thinsp;7.83\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.004\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e8.08\u0026thinsp;\u0026plusmn;\u0026thinsp;7.77\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eROM inversion (degree)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e13.75\u0026thinsp;\u0026plusmn;\u0026thinsp;6.78\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e26.25\u0026thinsp;\u0026plusmn;\u0026thinsp;9.64\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e12.50\u0026thinsp;\u0026plusmn;\u0026thinsp;7.76\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eROM eversion (degree)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e6.00\u0026thinsp;\u0026plusmn;\u0026thinsp;4.17\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e13.25\u0026thinsp;\u0026plusmn;\u0026thinsp;5.77\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.004\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e7.25\u0026thinsp;\u0026plusmn;\u0026thinsp;6.92\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"5\"\u003eSD: standard deviation; ROM: range of motion. The difference of ankle joint circumference is swelling. Swelling = (Circumference of injured side) - (Circumference of noninjured side), and the difference of angle = (ROM of noninjured side) - (ROM of injured side).\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tfoot\u003e\n\u003c/table\u003e\n\u003c/div\u003e\n\u003ch2\u003eGait Analysis: Comparison Between The Injured And Noninjured Side And Between Patients And Healthy Subjects\u003c/h2\u003e\n\u003cdiv id=\"Sec16\" class=\"Section2\"\u003e\n\u003ch2\u003e\u0026bull; Temporal-Spatial Parameters\u003c/h2\u003e\n\u003cp\u003eThe temporal-spatial gait parameters were as shown in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e. The standardized single-support time was expressed by the ratio of the single-support time to the total gait cycle to eliminate individual difference. The symmetry index is calculated using the formula: ((involved-uninvolved)/(involved\u0026thinsp;+\u0026thinsp;uninvolved)/2)\u0026sdot;100. An absolute value of symmetry index of zero indicates perfect symmetry and up to 5% difference between limbs is considered normal [\u003cspan class=\"CitationRef\"\u003e1\u003c/span\u003e].\u003c/p\u003e\n\u003cp\u003eCompared with the noninjured side, the step length, walking speed and single support time of the injured side in the patients were significantly decreased (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.008, 0.041, 0.008, Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e), while the step width was similar between both sides (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.434). Compared with the healthy subjects, patients demonstrated shorter step length (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001), wider step width (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001), slower walking speed (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001) and less single support time (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001).The symmetry indexes indicated asymmetry in patients and symmetry in healthy subjects. The symmetry index of the step length and single support time was significant different between the 2 groups (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.014, 0.028), while the symmetry index of step width and walking speed was similar between the 2 groups (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.397, 0.052).\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003ctable id=\"Tab3\" border=\"1\"\u003e\u003ccaption\u003e\n\u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n\u003cdiv class=\"CaptionContent\"\u003e\n\u003cp\u003eTemporal-spatial gait parameters of the patients with trimalleolar fractures and both sides of the healthy controls\u003c/p\u003e\n\u003c/div\u003e\n\u003c/caption\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eInjured side\u003c/p\u003e\n\u003cp\u003e(n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eNoninjured side (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eHealthy controls (n\u0026thinsp;=\u0026thinsp;24)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003e\u003cem\u003eP1\u003c/em\u003e value\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003e\u003cem\u003eP2\u003c/em\u003e value\u003c/p\u003e\n\u003c/th\u003e\n\u003c/tr\u003e\n\u003c/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eStep length (m)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.48\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.41\u0026thinsp;\u0026plusmn;\u0026thinsp;0.15\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.66\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eStep width (m)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.12\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.434\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eWalking speed (m/s)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.65\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.66\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e1.29\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.041\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSingle support time (%)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e16.27\u0026thinsp;\u0026plusmn;\u0026thinsp;5.63\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e20.40\u0026thinsp;\u0026plusmn;\u0026thinsp;2.71\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e32.27\u0026thinsp;\u0026plusmn;\u0026thinsp;2.96\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.008\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u0026lt;0.001\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSymmetry index step\u003c/p\u003e\n\u003cp\u003elength (%)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e21.44\u0026thinsp;\u0026plusmn;\u0026thinsp;26.34\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e-0.49\u0026thinsp;\u0026plusmn;\u0026thinsp;6.02\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.014\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSymmetry index step\u003c/p\u003e\n\u003cp\u003eWidth (%)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e-0.48\u0026thinsp;\u0026plusmn;\u0026thinsp;7.07\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e-4.65\u0026thinsp;\u0026plusmn;\u0026thinsp;14.17\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.397\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSymmetry index walking\u003c/p\u003e\n\u003cp\u003espeed (%)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e-3.74\u0026thinsp;\u0026plusmn;\u0026thinsp;4.01\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e-0.35\u0026thinsp;\u0026plusmn;\u0026thinsp;3.67\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.052\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eSymmetry index single\u003c/p\u003e\n\u003cp\u003esupport time (%)\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e-27.63\u0026thinsp;\u0026plusmn;\u0026thinsp;27.35\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e-0.94\u0026thinsp;\u0026plusmn;\u0026thinsp;7.23\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e0.028\u003c/p\u003e\n\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"6\"\u003eP1: injured side VS noninjured side, P2: injured side VS healthy controls\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tfoot\u003e\n\u003c/table\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec17\" class=\"Section3\"\u003e\n\u003ch2\u003e\u0026bull; Dynamic plantar pressure parameters\u003c/h2\u003e\n\u003cp\u003eCompared to the noninjured side, the injured side demonstrated lower peak plantar pressure for the T345 (injured side: 61.09\u0026thinsp;\u0026plusmn;\u0026thinsp;60.13 kpa, noninjured side: 112.27\u0026thinsp;\u0026plusmn;\u0026thinsp;79.12 kpa, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.022) and smaller contact area for the MF (injured side: 22.60\u0026thinsp;\u0026plusmn;\u0026thinsp;5.64 cm\u003csup\u003e2\u003c/sup\u003e, noninjured side: 25.87\u0026thinsp;\u0026plusmn;\u0026thinsp;7.90 cm\u003csup\u003e2\u003c/sup\u003e, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.038). The peak plantar pressure, contact area and contact time in other regions were similar in both sides (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;0.05).\u003c/p\u003e\n\u003cp\u003eAs shown in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e, compared to the healthy group, the trimalleolar fractures group demonstrated lower peak pressure for the HF, M2, M3, T2, T345 (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.040, \u0026lt;0.001,\u0026lt;0.001, 0.001, 0.003). The contact areas of the HF, M4, T1, T2, T345 in the trimalleolar fractures group were smaller (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001, 0.017, 0.004, 0.002,\u0026lt;0.001). The contact time for the HF and MF in the trimalleolar fractures group was increased (\u003cem\u003eP\u003c/em\u003e\u0026lt;0.001, 0.001), and the contact time for the T2, T345 was reduced (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.001,0.027). The total contact time of the patients and healthy group were (981.00\u0026thinsp;\u0026plusmn;\u0026thinsp;141.45) ms and (791.14\u0026thinsp;\u0026plusmn;\u0026thinsp;111.05) ms respectively, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.003.\u003c/p\u003e\n\u003cdiv class=\"gridtable\"\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003cdiv class=\"colspec\" align=\"left\"\u003e\u0026nbsp;\u003c/div\u003e\n\u003ctable id=\"Tab4\" border=\"1\"\u003e\u003ccaption\u003e\n\u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e\n\u003cdiv class=\"CaptionContent\"\u003e\n\u003cp\u003eComparison of dynamic plantar pressure features between the injured sides of patients with trimalleolar fractures and both sides of the healthy controls\u003c/p\u003e\n\u003c/div\u003e\n\u003c/caption\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth rowspan=\"2\" align=\"left\"\u003e\n\u003cp\u003eRegion\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003ePeak plantar pressure (kpa)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003eContact area (cm\u003csup\u003e2\u003c/sup\u003e)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"4\" align=\"left\"\u003e\n\u003cp\u003eContact time (%)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"1\" align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eTrimalleolar\u003c/p\u003e\n\u003cp\u003efractures (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eHealthy controls (n\u0026thinsp;=\u0026thinsp;24)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eTrimalleolar fractures (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eHealthy controls (n\u0026thinsp;=\u0026thinsp;24)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth align=\"left\"\u003e\n\u003cp\u003eTrimalleolar fractures (n\u0026thinsp;=\u0026thinsp;12)\u003c/p\u003e\n\u003c/th\u003e\n\u003cth colspan=\"4\" align=\"left\"\u003e\n\u003cp\u003eHealthy controls (n\u0026thinsp;=\u0026thinsp;24)\u003c/p\u003e\n\u003c/th\u003e\n\u003c/tr\u003e\n\u003c/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eHF\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e290.95\u0026thinsp;\u0026plusmn;\u0026thinsp;54.45*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e358.12\u0026thinsp;\u0026plusmn;\u0026thinsp;96.90\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e28.48\u0026thinsp;\u0026plusmn;\u0026thinsp;3.07*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e30.78\u0026thinsp;\u0026plusmn;\u0026thinsp;3.59\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e71.06\u0026thinsp;\u0026plusmn;\u0026thinsp;7.18*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e57.33\u0026thinsp;\u0026plusmn;\u0026thinsp;7.17\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eMF\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e127.68\u0026thinsp;\u0026plusmn;\u0026thinsp;39.75\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e155.37\u0026thinsp;\u0026plusmn;\u0026thinsp;47.08\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e22.60\u0026thinsp;\u0026plusmn;\u0026thinsp;5.64\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e25.81\u0026thinsp;\u0026plusmn;\u0026thinsp;5.32\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e72.18\u0026thinsp;\u0026plusmn;\u0026thinsp;7.22*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e66.10\u0026thinsp;\u0026plusmn;\u0026thinsp;5.57\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eM1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e253.00\u0026thinsp;\u0026plusmn;\u0026thinsp;138.61\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e277.83\u0026thinsp;\u0026plusmn;\u0026thinsp;101.60\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e11.20\u0026thinsp;\u0026plusmn;\u0026thinsp;2.24\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e11.93\u0026thinsp;\u0026plusmn;\u0026thinsp;1.20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e79.97\u0026thinsp;\u0026plusmn;\u0026thinsp;9.04\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e82.16\u0026thinsp;\u0026plusmn;\u0026thinsp;3.76\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eM2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e226.22\u0026thinsp;\u0026plusmn;\u0026thinsp;144.40*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e597.62\u0026thinsp;\u0026plusmn;\u0026thinsp;292.83\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e9.05\u0026thinsp;\u0026plusmn;\u0026thinsp;1.23\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e9.31\u0026thinsp;\u0026plusmn;\u0026thinsp;0.77\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e85.32\u0026thinsp;\u0026plusmn;\u0026thinsp;6.57\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e84.62\u0026thinsp;\u0026plusmn;\u0026thinsp;3.61\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eM3\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e245.45\u0026thinsp;\u0026plusmn;\u0026thinsp;147.56*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e455.67\u0026thinsp;\u0026plusmn;\u0026thinsp;149.56\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e9.97\u0026thinsp;\u0026plusmn;\u0026thinsp;1.40\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e10.69\u0026thinsp;\u0026plusmn;\u0026thinsp;0.92\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e86.76\u0026thinsp;\u0026plusmn;\u0026thinsp;6.68\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e85.55\u0026thinsp;\u0026plusmn;\u0026thinsp;3.11\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eM4\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e192.50\u0026thinsp;\u0026plusmn;\u0026thinsp;113.27\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e246.37\u0026thinsp;\u0026plusmn;\u0026thinsp;39.19\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e7.97\u0026thinsp;\u0026plusmn;\u0026thinsp;1.04*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e8.75\u0026thinsp;\u0026plusmn;\u0026thinsp;0.74\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e83.40\u0026thinsp;\u0026plusmn;\u0026thinsp;6.60\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e83.71\u0026thinsp;\u0026plusmn;\u0026thinsp;3.31\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eM5\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e125.22\u0026thinsp;\u0026plusmn;\u0026thinsp;72.11\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e184.00\u0026thinsp;\u0026plusmn;\u0026thinsp;96.74\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e5.03\u0026thinsp;\u0026plusmn;\u0026thinsp;0.58\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e5.43\u0026thinsp;\u0026plusmn;\u0026thinsp;0.68\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e76.60\u0026thinsp;\u0026plusmn;\u0026thinsp;6.32\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e75.96\u0026thinsp;\u0026plusmn;\u0026thinsp;4.67\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eT1\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e300.18\u0026thinsp;\u0026plusmn;\u0026thinsp;286.80\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e417.42\u0026thinsp;\u0026plusmn;\u0026thinsp;141.87\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e7.45\u0026thinsp;\u0026plusmn;\u0026thinsp;2.31*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e10.04\u0026thinsp;\u0026plusmn;\u0026thinsp;0.99\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e75.26\u0026thinsp;\u0026plusmn;\u0026thinsp;20.24\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e73.35\u0026thinsp;\u0026plusmn;\u0026thinsp;11.52\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eT2\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e79.27\u0026thinsp;\u0026plusmn;\u0026thinsp;81.91*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e211.75\u0026thinsp;\u0026plusmn;\u0026thinsp;104.20\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e2.25\u0026thinsp;\u0026plusmn;\u0026thinsp;1.37*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e3.92\u0026thinsp;\u0026plusmn;\u0026thinsp;0.54\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e43.78\u0026thinsp;\u0026plusmn;\u0026thinsp;20.71*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e65.22\u0026thinsp;\u0026plusmn;\u0026thinsp;13.57\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003ctr\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003eT345\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e61.09\u0026thinsp;\u0026plusmn;\u0026thinsp;60.13*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e148.08\u0026thinsp;\u0026plusmn;\u0026thinsp;78.35\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e2.81\u0026thinsp;\u0026plusmn;\u0026thinsp;2.17*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e6.84\u0026thinsp;\u0026plusmn;\u0026thinsp;2.03\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\n\u003cp\u003e\u003cstrong\u003e42.57\u0026thinsp;\u0026plusmn;\u0026thinsp;26.22*\u003c/strong\u003e\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd align=\"left\"\u003e\n\u003cp\u003e63.40\u0026thinsp;\u0026plusmn;\u0026thinsp;12.06\u003c/p\u003e\n\u003c/td\u003e\n\u003ctd colspan=\"2\" align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n\u003c/tr\u003e\n\u003c/tbody\u003e\n\u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003eHF: hindfoot, MF: medial midfoot, M1: the first metatarsal head, M2: the second metatarsal head, M3: the third metatarsal head, M4: the fourth metatarsal head, M5: the fifth metatarsal head, T1: hallux, T1: the second toe, T3-5: the third to fifth toes\u003c/p\u003e\n\u003cdiv id=\"Sec18\" class=\"Section4\"\u003e\n\u003cp\u003e*P\u0026thinsp;\u0026lt;\u0026thinsp;0.05\u003c/p\u003e\n\u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec19\" class=\"Section3\"\u003e\n\u003ch2\u003e\u0026bull; sEMG\u003c/h2\u003e\n\u003cp\u003eThe integrated EMG (%) (IEMG) of TA of the injured side (10.84\u0026thinsp;\u0026plusmn;\u0026thinsp;4.59) in the patients was significantly larger than the noninjured side (8.93\u0026thinsp;\u0026plusmn;\u0026thinsp;4.56), \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.014. While the IEMG (%) of PL (injured side: 29.51\u0026thinsp;\u0026plusmn;\u0026thinsp;20.43, noninjured side: 54.35\u0026thinsp;\u0026plusmn;\u0026thinsp;57.27, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.117), GL (injured side: 27.91\u0026thinsp;\u0026plusmn;\u0026thinsp;17.78, noninjured side: 32.13\u0026thinsp;\u0026plusmn;\u0026thinsp;19.75, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.581), GM (injured side: 24.78\u0026thinsp;\u0026plusmn;\u0026thinsp;17.83, noninjured side: 34.44\u0026thinsp;\u0026plusmn;\u0026thinsp;22.77, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.124) and S (injured side: 21.30\u0026thinsp;\u0026plusmn;\u0026thinsp;12.65, noninjured side: 21.94\u0026thinsp;\u0026plusmn;\u0026thinsp;14.41, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.922) were not significant different between both sides.\u003c/p\u003e\n\u003cp\u003eDuring walking, the IEMG (%) of TA, PL were significantly higher in the patients (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.002, 0.008) than in the healthy controls, while no differences were identified between groups for IEMG (%) of GL, GM and S (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e).\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec21\" class=\"Section3\"\u003e\n\u003ch2\u003e\u0026bull; Correlations analysis\u003c/h2\u003e\n\u003cp\u003eThe symmetry index of step width and walking speed were highly inversely correlated with the difference of ankle inversion ROM between two sides (\u003cem\u003eR\u003c/em\u003e=-0.750, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.005, Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003eA; \u003cem\u003eR\u003c/em\u003e=-0.700, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.011, Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003eB). The symmetry indexes were not correlated with other clinical assessments of the ankle (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;0.050).\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003c/div\u003e\n\u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eThis study indicated that at an average of 4.5-months post-surgery, patients with trimalleolar fractures showed poor OMAS results, and their injured ankles were swollen, and the passive ROM were decreased. During gait analysis, patients demonstrated abnormal gait compared with healthy controls, and an asymmetrical gait pattern was seen in patients. Compared with healthy controls, the abnormal performance of patients in plantar pressure distribution were concentrated in hindfoot and forefoot, and patients also showed abnormal muscle activity of TA and PL. Furthermore, the passive inversion ROM was highly correlated to symmetry index of step width and walking. This study was the first to indicate remaining detailed gait deficits in patients with trimalleolar fractures. In addition, gait parameters were correlated with clinical outcomes in patients with trimalleolar fractures for the first time.\u003c/p\u003e \u003cp\u003eOMAS are usually used as a reliable and valid outcome measure after an ankle fracture[\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e], and based on the total score, ankle function of patients could be divided into four grades: excellent (OMAS:100 to 91 points), good (OMAS:90 to 61 points), fair (OMAS:60 to 31 points) and poor (OMAS:30 to 0 points). [\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e] In this study, the mean OMAS was 56, indicating that patients with trimalleolar fractures reported fair ankle function. According to the study, the disability of running and jumping contributed most to the total score. Several studies also investigated the OMAS of ankle fractures patients, and they showed better results than our study. Oguzhan Tano glu et al. [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]compared the effect of a 1-stage surgery for the unstable malleolar fracture dislocations with the 2-stage surgery. The two group all included patients with isolated malleolar fractures, bimalleolar fractures and trimalleolar fractures. And the duration of follow-up of the two group was 21.7 and 19.2 months respectively. The mean OMAS was 87.8 for the 1-stage surgery group and 83.2 for the 2-stage surgery. Mareen Braunstein et al. [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e]demonstrated functional outcomes after 1 year of arthroscopically assisted ankle fracture treatment, and they reported a mean OMAS of 85 for trimalleolar fractures. The poor OMAS results for trimalleolar fractures in this study might be mainly attributed to the short length of the postoperative follow-up period. This study investigated the clinical outcomes after 4.5 months postoperatively, while other studies evaluated the long-term (more than 1 year) clinical outcomes.\u003c/p\u003e \u003cp\u003eThis study indicated that patients with trimalleolar fractures remained physical impairments postoperatively. Compared with the noninjured side, patients represented ankle swelling, and a decrease in passive ROM on the injured side. Ankle swelling is a common and long-standing complication after surgery. It was reported that more than half of the patients following unimalleolar and bimalleolar ankle fractures presented stiffness, swelling and pain [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. 60% or more of the patients 65 years or older reported ankle pain, swelling and problems when using stairs and reduced activities of daily life one year after ankle fractures [\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. Shah et al.[\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e]demonstrated that around 45% of 69 patients with Weber B and C ankle fractures still had ankle swelling at 5 years after the injury. Our study investigated the passive ROM, and mean (sd) angle of dorsiflexion, plantarflexion, inversion and eversion of the injured leg on the trimalleolar fractures patients was 7.08 (3.91) degrees, 33.33 (8.07) degrees, 13.75 (6.78) degrees and 6.00 (4.17) degrees respectively. Ganit Segal et al. [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e] also measured the passive ankle ROM of patients with trimalleolar fractures in the sagittal (dorsiflexion/ plantar flexion) and coronal plane (inversion/eversion), and the ROM was \u0026minus;\u0026thinsp;0.8 (7.6) degrees, 40.6 (7.5) degrees, 5.6 (3.6) degrees and 2.5 (4.0) degrees respectively. The result was a little different from our study, might due to the assessment point. Ganit Segal et al. [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e] measured ROM at 64.5 days from injury, which was earlier than ours (4.5 months). There was also a study evaluated ROM during activities. van Hoeve, S et al. [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e] found that compared with the healthy subjects (12.59 ̊ \u0026plusmn; 3.73 ̊), the ROM during gait in patients (7.13 ̊ \u0026plusmn; 2.55 ̊) with trimalleolar ankle fractures decreased significantly. After ankle fractures, the uncoagulated hemorrhage leads to the rapid increase of intra-articular pressure, which causes abrupt joint swelling, pain and limited mobility [24]. Presence of soft tissue damages such as tendon and ligamental injuries can cause chronic swelling and stiffness therefore resulting in the dismal outcome [3]. These complications might alter the gait.\u003c/p\u003e \u003cp\u003eThe present study showed that patients with trimalleolar fractures presented compromised gait pattern. The temporal-spatial parameters of the injured side were significantly different from the noninjured side and healthy subjects, except no difference exist in step width between both sides. This is in line with other research findings. Two studies investigated the gait parameters of patients with trimalleolar fractures, and compared those with healthy group. They found all gait parameters were significantly below the normal range[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. Andrew F. Tyler et al. [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e] also showed that the gait characteristics of ankle fracture patients were more similar to healthy elderly patients. However, these two studies only investigated the differences between patients and healthy subjects, but did not compare the gait parameters of the injured side with the noninjured side. Ganit Segal et al. [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]also examined limb symmetry of the gait patterns, and found significant asymmetry in step length and single limb support. These findings were consistent with this study. And by correlation analysis in this study, step asymmetry might be related to the difference of ankle inversion ROM between two sides. All these results presented that patients with trimalleolar fractures did not achieve restoration of normal physiologic gait in the short-term, and patients adopted a simple security strategy with a reduction of walking speed[\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe differences of features of plantar pressure distributions and sEMG in both sides also indicated asymmetries in gait in patients with trimalleolar fractures. The plantar pressure in the T345 and the contact area of MF of the injured side were significantly smaller than those of the noninjured side, and this was probably due to a more cautious and compensatory walking pattern, by further biasing the center of gravity to the noninjured side. Sjoerd Kolk et al.[\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e] also showed subtle asymmetries in gait kinetics and kinematics between the operated and non-operated limbs, and they considered that patients performed a more cautious walking pattern and an integral strategy. Plantar pressures of other type ankle fractures such as pilon fractures, calcaneal fractures were also asymmetry, and adhesion or conduction disorders at the tibia may be causes of abnormal plantar pressure [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e, \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eCompared with normal healthy subjects, patients with trimalleolar fracture performed abnormal gait during walking support period. Patients tended to step more cautiously on the injured heel, showing smaller peak plantar pressure in HF, and this might be due to pain or psychological factors such as fear or worry of reinjury [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e]. For patients, the contact time (%) of HF and MF and total contact time were significantly increased, and it might be associated with lower ankle stability: for patients with trimalleolar fractures, the lateral, medial and posterior malleolus were injured, probably impairing the ankle stability, and patients needed more time to maintain ankle stability [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. The muscle activity on the TA and PL of the injured side was significantly larger than those in the noninjured, also indicating the ankle stability of the injured side decreased, because muscular co-contraction of TA and PL was increased to stabilize the ankle joint [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e]. And in this study, the step width was significantly smaller than that of healthy controls, also showing the walk stability decreased in patients. The plantar pressure features (smaller peak plantar pressure and contact area, shorter contact time) in the forefoot demonstrated that the propulsion ability during walking significantly decreased in patient, and this could decrease the walking speed. In this study, the IEMG of PL of patients was significantly increased, indicating the muscle ability decreased. Therefore, it was reasonable to speculate that the abnormal gait features in the forefoot might be related to the decreased ability of PL. During normal walking, the plantar pressure in the first metatarsal head plays a key role in pushing off [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e]. And the PL is essential for maintaining ankle stability and plays an important role in the push-off stage. The PL origins at the proximal tibia and fibula, and inserts at the first metatarsal and medial cuneiform [\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e]. Except for contributing to 63% of eversion strength, the PL is helpful to initiate pronation and stabilize first ray during propulsion phases of gait [\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e].\u003c/p\u003e"},{"header":"Limitations","content":"\u003cp\u003eThere were some limitations in this study. First, this study did not divide the patients into \u0026ldquo;unfixed posterior malleoli group\u0026rdquo; and \u0026ldquo;fixed Posterior malleoli group\u0026rdquo;. However, it was demonstrated that fixation of the posterior malleolus particularly did not appear to improve gait characteristics. Therefore, the patients in this study were not further grouped into two subgroups. Second, the participants in this study were asked to walk at their natural speed, and these values in healthy subjects were found to be faster when compared to the patients, which may affect the comparisons between the other gait biomechanics parameters. Future research should examine the difference in gait patterns when patients walk at the same speed. Third, this was a cross-sectional evaluation of patients following ankle fracture. Future research should investigate long-term differences in clinical outcomes and gait patterns, to evaluate the function of patients with trimalleolar fractures comprehensively.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003ePatients with trimalleolar fractures showed poor PROM results, ankle swelling and smaller passive ROM. Compared to healthy controls, patients showed altered temporal-spatial, plantar pressure and sEMG parameters. Gait asymmetries were correlated to the difference of ankle inversion ROM between two sides. The ankle stability of patients declined, and deficits in TA and PL muscle ability might contribute to it. The propulsion ability of patients during walking significantly decreased, and this was mainly correlated to the deficit in PL ability. Restoring complete muscle functions and improving passive ankle ROM are significant to guarantee the recovery of a normal gait pattern, which is helpful to the recovery of normal activities of daily life and sports.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eThis study was performed in accordance with the Declaration of Helsinki. This study was approved by the Medical Ethics Committee of Shanghai Sunshine Rehabilitation Center (No: YZ2019-001). Before the experiment started, all participants had detailed procedures introduced and signed the informed consent documents. The written informed consent was obtained.\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eAll data generated or analysed during this study are included in this published article.\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eThe authors do not have any conflicts of interest to disclose.\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eThis work was supported by the National Key Research and Development Program of China [grant number 2018YFF0300504]; Science and Technology Commission of Shanghai Municipality [grant number 18441907200].\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003e(I) Conception and design: Ting Zhu, Shaobai Wang; (II) Provision of study materials or patients: Rongzhou Zhong, Hua Zhai;(III) Collection and assembly of data: Ting Zhu, Ya Wang, Wenjin Wang; (IV) Data analysis and interpretation: Ting Zhu, Ya Wang, Fei Tian; (V) Manuscript writing: Ting Zhu; (VI) Final approval of manuscript: Hua Zhai, Shaobai Wang.\u003c/p\u003e\n\u003cul\u003e\n \u003cli\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/li\u003e\n\u003c/ul\u003e\n\u003cp\u003eThanks to all the subjects who participated in this study. We would like to acknowledge Miss Xia Ma and Mrs. Yujuan Zhang for their assistance with recruiting subjects.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eG. Segal, A. Elbaz, A. Parsi, Z. Heller, E. Palmanovich, M. Nyska, et al., Clinical outcomes following ankle fracture: a cross-sectional observational study, Journal of foot and ankle research. 2014;7(1): 50.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eG. Testa, M. Ganci, M. Amico, G. Papotto, S.M.C. Giardina, G. Sessa, et al., Negative prognostic factors in surgical treatment for trimalleolar fractures, European journal of orthopaedic surgery \u0026amp; traumatology: orthopedie traumatology. 2019;29(6): 1325\u0026ndash;1330.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eC.C. Hong, N. Nashi, S. Prosad Roy, K.J. Tan, Impact of trimalleolar ankle fractures: how do patients fare post-operatively?, Foot and ankle surgery: official journal of the European Society of Foot and Ankle Surgeons. 2014;20(1): 48\u0026ndash;51.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. van Hoeve, M. Houben, J. Verbruggen, P. Willems, K. Meijer, M. Poeze, Gait analysis related to functional outcome in patients operated for ankle fractures, Journal of orthopaedic research: official publication of the Orthopaedic Research Society. 2019;37(7): 1658\u0026ndash;1666.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eR. Wang, C.K. Thur, E.M. Gutierrez-Farewik, P. Wretenberg, E. Brostr\u0026ouml;m, One year follow-up after operative ankle fractures: a prospective gait analysis study with a multi-segment foot model, Gait \u0026amp; posture. 2010;31(2): 234\u0026ndash;40.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eA. Tyler, T. Rose, S. Day, J. Kenia, A. Horan, S. Mehta, et al., Comparison of Spatiotemporal Gait Parameters Following Operative Treatment of Trimalleolar Ankle Fractures vs Healthy Controls, Foot \u0026amp; Ankle Orthopaedics. 2020;5: 247301142093105.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eI.M. Punt, J.-L. Ziltener, M. Laidet, S. Armand, L. Allet, Gait and Physical Impairments in Patients With Acute Ankle Sprains Who Did Not Receive Physical Therapy, PM\u0026amp;R 2015;7(1): 34\u0026ndash;41.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eJ.M. Kane, S. Coleman, J.W. Brodsky, Kinematics and Function of Total Ankle Replacements Versus Normal Ankles, Foot and ankle clinics. 2017;22(2): 241\u0026ndash;249.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. Blair, M.J. Lake, R. Ding, T. Sterzing, Magnitude and variability of gait characteristics when walking on an irregular surface at different speeds, Human movement science. 2018;59: 112\u0026ndash;120.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eA.K. Buldt, S. Forghany, K.B. Landorf, P. Levinger, G.S. Murley, H.B. Menz, Foot posture is associated with plantar pressure during gait: A comparison of normal, planus and cavus feet, Gait \u0026amp; posture. 2018;62: 235\u0026ndash;240.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eM.A. Feger, L. Donovan, J.M. Hart, J. Hertel, Lower Extremity Muscle Activation in Patients With or Without Chronic Ankle Instability During Walking, Journal of Athletic Training. 2015;50(4): 350\u0026ndash;357.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eI. Campanini, C. Disselhorst-Klug, W.Z. Rymer, R. Merletti, Surface EMG in Clinical Assessment and Neurorehabilitation: Barriers Limiting Its Use, Frontiers in neurology. 2020;11: 934.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eC. Olerud, H. Molander, A scoring scale for symptom evaluation after ankle fracture, Archives of orthopaedic and traumatic surgery. Archiv fur orthopadische und Unfall-Chirurgie. 1984;103(3): 190\u0026ndash;4.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eM.L. Pugia, C.J. Middel, S.W. Seward, J.L. Pollock, R.C. Hall, L. Lowe, et al., Comparison of acute swelling and function in subjects with lateral ankle injury, The Journal of orthopaedic and sports physical therapy. 2001;31(7): 384\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eR.A. Elveru, J.M. Rothstein, R.L. Lamb, Goniometric reliability in a clinical setting. Subtalar and ankle joint measurements, Physical therapy. 1988;68(5): 672\u0026ndash;7.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eI.M. Punt, J.L. Ziltener, M. Laidet, S. Armand, L. Allet, Gait and physical impairments in patients with acute ankle sprains who did not receive physical therapy, PM \u0026amp; R: the journal of injury, function, and rehabilitation. 2015;7(1): 34\u0026ndash;41.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eP. Aleixo, J. Vaz Patto, A. Cardoso, H. Moreira, J. Abrantes, Ankle kinematics and kinetics during gait in healthy and rheumatoid arthritis post-menopausal women, Somatosensory \u0026amp; motor research. 2019;36(2): 171\u0026ndash;178.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eA.M. Garratt, M.G. Naumann, U. Sigurdsen, S.E. Utv\u0026aring;g, K. Stavem, Evaluation of three patient reported outcome measures following operative fixation of closed ankle fractures, BMC musculoskeletal disorders. 2018;19(1): 134.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. G\u0026ouml;rtz, A.J. De Young, W.D. Bugbee, Fresh osteochondral allografting for osteochondral lesions of the talus, Foot \u0026amp; ankle international. 2010;31(4): 283\u0026ndash;90.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eO. Tanoğlu, M.B. G\u0026ouml;kg\u0026ouml;z, A. \u0026Ouml;zmeri\u0026ccedil;, K.B. Alemdaroğlu, Two-Stage Surgery for the Malleolar Fracture-Dislocation With Severe Soft Tissue Injuries Does Not Affect the Functional Results, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2019;58(4): 702\u0026ndash;705.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eM. Braunstein, S.F. Baumbach, M. Urresti-Gundlach, L. Borgmann, W. B\u0026ouml;cker, H. Polzer, Arthroscopically Assisted Treatment of Complex Ankle Fractures: Intra-articular Findings and 1-Year Follow-Up, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2020;59(1): 9\u0026ndash;15.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eG. Nilsson, K. Jonsson, C. Ekdahl, M. Eneroth, Outcome and quality of life after surgically treated ankle fractures in patients 65 years or older, BMC musculoskeletal disorders. 2007;8: 127.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eN.H. Shah, R.O. Sundaram, A. Velusamy, I.J. Braithwaite, Five-year functional outcome analysis of ankle fracture fixation, Injury. 2007;38(11): 1308\u0026ndash;1312.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eC. Liu, J. Guo, Q. Cui, D. Li, Y. Zeng, Clinical and imaging analysis of subclinical hemophilia combined with coxarthrosis: case report and literature review, Springerplus. 2016;5(1): 2058\u0026ndash;2058.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. Kolk, R. Fluit, J. Luijten, P.J. Heesterbeek, A.C. Geurts, N. Verdonschot, et al., Gait and lower limb muscle strength in women after triple innominate osteotomy, BMC musculoskeletal disorders. 2015;16: 68.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. van Hoeve, J. de Vos, J.P. Verbruggen, P. Willems, K. Meijer, M. Poeze, Gait Analysis and Functional Outcome After Calcaneal Fracture, The Journal of bone and joint surgery. American volume. 2015;97(22): 1879\u0026ndash;88.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eS. Jandova, J. Pazour, M. Janura, Comparison of Plantar Pressure Distribution During Walking After Two Different Surgical Treatments for Calcaneal Fracture, The Journal of foot and ankle surgery: official publication of the American College of Foot and Ankle Surgeons. 2019;58(2): 260\u0026ndash;265.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eK. Sasaki, R.R. Neptune, Differences in muscle function during walking and running at the same speed, Journal of biomechanics. 2006;39(11): 2005\u0026ndash;13.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eF. Pu, W. Ren, H. Fu, X. Zheng, M. Yang, Y.K. Jan, et al., Plantar blood flow response to accumulated pressure stimulus in diabetic people with different peak plantar pressure: a non-randomized clinical trial, Medical \u0026amp; biological engineering \u0026amp; computing. 2018;56(7): 1127\u0026ndash;1134.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eD. Koh, L. Liow, J. Cheah, K. Koo, Peroneus longus tendon rupture: A case report, World journal of orthopedics. 2019;10(1): 45\u0026ndash;53.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-musculoskeletal-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bmsd","sideBox":"Learn more about [BMC Musculoskeletal Disorders](http://bmcmusculoskeletdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://author-welcome.nature.com/12891","title":"BMC Musculoskeletal Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Ankle trimalleolar fracture, Clinical assessments, Biomechanics, Gait","lastPublishedDoi":"10.21203/rs.3.rs-1494636/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1494636/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground: \u003c/strong\u003eTrimalleolar fracture is a common ankle fracture with serious complications and costly healthcare problem. Most studies used clinical assessments to evaluate the functional status of the patients.\u003cstrong\u003e \u003c/strong\u003eAlthough clinical assessments are valid, they are static and subjective. Dynamic, objective and precise evaluations such as gait analysis are needed. Ankle biomechanics studies on gait in patients with trimalleolar fractures are still rare. This study aimed to investigate the clinical outcomes and gait biomechanics in patients with trimalleolar fractures and compared to healthy controls. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003e This was a cross-sectional study. 12 patients with trimalleolar fractures were recruited, and 12 healthy people served as controls. All patients underwent clinical assessments: Olerud and Molander ankle score (OMAS), ankle swelling and passive range of motion (ROM) of ankle, and completed gait biomechanical analysis: temporal-spatial parameters, plantar pressure distributions, and surface electromyography (sEMG). The control group only performed gait test. \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003ePatients had poor outcomes of clinical assessments. During gait analysis, patients presented compromised gait patterns: shorter step length, larger step width, slower walking speed and shorter single support compared to healthy controls (\u003cem\u003ep \u003c/em\u003e\u0026lt; 0.001), and patients showed asymmetrical gait. Symmetry index of step width and walking speed were mainly correlated with the difference of ankle inversion ROM between two sides (\u003cem\u003eR\u003c/em\u003e=-0.750, \u003cem\u003eP\u003c/em\u003e=0.005; \u003cem\u003eR\u003c/em\u003e=-0.700, \u003cem\u003eP\u003c/em\u003e=0.011). During walking, patients showed abnormal dynamic plantar pressure features (mainly in the hindfoot and forefoot regions), and the IEMG (integrated electromyography) of tibial anterior muscle (TA) and peroneal longus muscle (PL) were larger than healthy controls (\u003cem\u003eP\u003c/em\u003e=0.002, 0.050). \u003c/p\u003e\u003cp\u003e\u003cstrong\u003eConclusions: \u003c/strong\u003ePatients with trimalleolar fractures showed physical impairments of the ankle, and presented altered gait parameters compared to healthy subjects. The ankle stability of patients declined, and deficits in TA and PL muscle ability might contribute to it. Restoring complete muscle functions and improving passive ankle ROM are significant to promote the recovery of a normal gait pattern.\u003c/p\u003e","manuscriptTitle":"Clinical Assessments and Gait Performance in Patients with Trimalleolar Fractures","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-04-01 20:18:54","doi":"10.21203/rs.3.rs-1494636/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2022-05-10T12:50:54+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2022-05-04T14:12:05+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"b5990f62-ca94-4750-b62e-5a644a8f8cec","date":"2022-04-22T11:04:05+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2022-04-22T10:24:49+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2022-04-07T05:48:39+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2022-03-30T17:47:12+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2022-03-30T17:43:32+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Musculoskeletal Disorders","date":"2022-03-27T15:28:42+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-musculoskeletal-disorders","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bmsd","sideBox":"Learn more about [BMC Musculoskeletal Disorders](http://bmcmusculoskeletdisord.biomedcentral.com/)","snPcode":"","submissionUrl":"https://author-welcome.nature.com/12891","title":"BMC Musculoskeletal Disorders","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"ce23ab23-6973-4a81-8d5d-cbdb05d60456","owner":[],"postedDate":"April 1st, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2022-07-01T08:14:15+00:00","versionOfRecord":[],"versionCreatedAt":"2022-04-01 20:18:54","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1494636","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1494636","identity":"rs-1494636","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}