Research article Evaluation of Cytotoxicity Induced by Kalanchoe pinnata Leaf Extraction Solvents: Exploring the Impact of Incubation Period on In vitro Survival of Mice Spermatozoa

Research article Evaluation of Cytotoxicity Induced by Kalanchoe pinnata Leaf Extraction Solvents: Exploring the Impact of Incubation Period on In vitro Survival of Mice Spermatozoa | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 4 August 2025 V1 Latest version Share on Research article Evaluation of Cytotoxicity Induced by Kalanchoe pinnata Leaf Extraction Solvents: Exploring the Impact of Incubation Period on In vitro Survival of Mice Spermatozoa Authors : Hanim YU , Vetriselvan Subramaniyan [email protected] , Rokeya Sultana , Neeraj Kumar Fuloria , Mahendran Sekar , Shivkanya Fuloria , Ainil Hawa Jasni 0000-0002-1013-1368 , Yuan Seng Wu , and Siddarthan Selvaraj Authors Info & Affiliations https://doi.org/10.22541/au.175431321.11474281/v1 142 views 83 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract The effects of cytotoxic solvents with concentrations ranging from 0.1 to 3.0 g/100 mL in Kalanchoe pinnata (Kp) extract were assessed across various incubation periods in the survival of mice spermatozoa in vitro. The objective of this study was to examine morphology, spermatozoa abnormalities, motility, and swim-up analysis. Eosin Y stain solution was employed to classify morphology, distinguishing between normal, abnormal head, abnormal midpiece, and abnormal tail in mice spermatozoa subjected to different concentrations of Kp extraction, solvent types, and incubation periods. The highest morphology analysis was observed in water solvent with 0.1% Kp concentration extract after a 1-hour incubation period, registering at 96.6 ± 1.20%. Motility analysis revealed rapid progressive activity in mice spermatozoa treated with water solvent and 0.1% Kp extract after an 1-hour incubation period. Swim-up analysis, assessing the viability of mice spermatozoa through absorbance percentage, indicated the highest values in water solvent with Kp extract (90 ± 1.21%) after incubation. In summary, the concentration, type of solvent, and incubation period of Kp extract in vitro demonstrated varying effects on cytotoxicity, mice spermatozoa morphology, progressive motility, and swim-up percentages. This research suggests potential avenues for further exploration of Kalanchoe pinnata leaf extracts in the development of novel therapeutic applications to address reproductive toxicity in global health. Supplementary Material File (exploring the impact of incubation period on in vitro survival of mice spermatozoa v4.docx) Download 3.43 MB Information & Authors Information Version history V1 Version 1 04 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords kalanchoe pinnata leaf cytotoxicity abnormalities mice spermatozoa morphology ethanol solvent kalanchoe pinnata extract mice spermatozoa motility water solvent kalanchoe pinnata extract Authors Affiliations Hanim YU Monash University Malaysia Jeffrey Cheah School of Medicine and Health Sciences View all articles by this author Vetriselvan Subramaniyan [email protected] Sunway University Department of Biological Sciences View all articles by this author Rokeya Sultana Yenepoya (Deemed to be University) Yenepoya Research Centre View all articles by this author Neeraj Kumar Fuloria AIMST University View all articles by this author Mahendran Sekar Monash University Malaysia School of Pharmacy View all articles by this author Shivkanya Fuloria AIMST University View all articles by this author Ainil Hawa Jasni 0000-0002-1013-1368 Sunway University Department of Biological Sciences View all articles by this author Yuan Seng Wu Sunway University Department of Biological Sciences View all articles by this author Siddarthan Selvaraj Universiti Malaya View all articles by this author Metrics & Citations Metrics Article Usage 142 views 83 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Hanim YU, Vetriselvan Subramaniyan, Rokeya Sultana, et al. Research article Evaluation of Cytotoxicity Induced by Kalanchoe pinnata Leaf Extraction Solvents: Exploring the Impact of Incubation Period on In vitro Survival of Mice Spermatozoa. Authorea . 04 August 2025. DOI: https://doi.org/10.22541/au.175431321.11474281/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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