Cortical and subcortical glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons

Cortical and subcortical glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Cortical and subcortical glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons Auguste Vadisiute, Elise Meijer, Rajeevan Therpurakal, Marissa Mueller, and 8 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3335293/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 09 Oct, 2024 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Abstract Communication between neurons and glial cells are crucial to establish and maintain brain function. Some of these interactions are activity-dependent, yet it remains unexplored how acute changes in neuronal activity affect neuron-to-glial dynamics. Here, we use excitatory and inhibitory designer receptors exclusively activated by designer drugs (DREADD) based chemogenetic methods to study the effects of acute manipulations of a subpopulation of layer 5 cortical projection and dentate gyrus neurons in adult (Rbp4 Cre ) mouse brain after 90 minutes. We show that acute chemogenetic neuronal activation leads to microglia hyperactivation, reactive astrogliosis, increased parvalbumin expression in interneurons and lower synaptic marker expression. Whereas acute chemogenetic ‘silencing’ of the same neurons reduce microglia activity, inhibit astrocytic reactivity, increase synaptic density, but has no effect on parvalbumin expression. Acute changes in neuronal activity elicit rapid effects on the interactions between glial cells, glutamatergic and GABAergic neuronal networks. Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Sourcedata.xlsx Supplementary Dataset 2 1Supplementaryfigure1Control600DPI.png 2Supplementaryfigure2ExcitatoryDREADDinterneuronssubcorticalregions600DPI.png 3Supplementaryfigure3InhibitoryDREADDinterneuronssubcorticalregions600DPI.png SupplementaryInformation.docx Cite Share Download PDF Status: Published Journal Publication published 09 Oct, 2024 Read the published version in Communications Biology → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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