Anti-Allergic Effects of Chamaecrista nomame Extract and Luteolin in an OVA-Induced Asthma Mouse Model

Anti-Allergic Effects of Chamaecrista nomame Extract and Luteolin in an OVA-Induced Asthma Mouse Model | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 15 January 2025 V1 Latest version Share on Anti-Allergic Effects of Chamaecrista nomame Extract and Luteolin in an OVA-Induced Asthma Mouse Model Authors : Tae Kyeom Kang , Myungsuk Kim , Youngsang Yoo , Tam Thi Le , Geon Park , Yuna Jung , Hyobin Jeong , Wook-Bin Lee 0000-0003-4712-5890 [email protected] , and Sang Hoon Jung Authors Info & Affiliations https://doi.org/10.22541/au.173697405.55741552/v1 350 views 99 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background : Asthma is a chronic inflammatory disease of the respiratory tract characterized by airway inflammation, mucus hypersecretion, and tissue remodeling. Mast cells play a pivotal role in these processes, making them important targets for therapeutic intervention. Natural products, such as Chamaecrista nomame , known for their antioxidant and anti-inflammatory properties, are potential alternatives to conventional asthma treatments. Methods : This study screened 302 plant extracts to identify those with anti-degranulation activity, selecting an extract of C. nomame (CN) as a promising candidate. The anti-allergic effects of CN were evaluated in vitro using RBL-2H3 mast cells and in vivo using an ovalbumin (OVA)-induced asthma mouse model. Parameters assessed included histopathological changes, inflammatory cell infiltration in bronchoalveolar lavage fluid (BALF), cytokine levels, and OVA-specific IgE levels. Key compounds in CN were identified, with luteolin as a focus. RNA sequencing and gene expression analysis were conducted on bone marrow-derived mast cells (BMMCs) to elucidate molecular mechanisms. The effects of CN and luteolin on cytokine production were evaluated ex vivo in house dust mite (HDM)-stimulated peripheral blood mononuclear cells (PBMCs). Results : CN significantly inhibited mast cell degranulation and cytokine release in vitro . In the OVA-induced asthma model, CN administration reduced airway inflammation, immune cell infiltration in BALF, and OVA-specific IgE and cytokine levels, comparable to dexamethasone. Luteolin downregulated NF-κB, MAPK, and TNF signaling pathways in BMMCs. CN with luteolin significantly reduced HDM-induced cytokine production in PBMCs. Conclusion : CN, particularly luteolin, exhibits potent antiallergic properties, indicating its potential as an alternative therapy for allergic asthma. Supplementary Material File (manuscript_cnl_asthma_luteolin_endnote_for allergy journal_20250114.docx) Download 443.12 KB Information & Authors Information Version history V1 Version 1 15 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords allergy treament asthma biologics inflammation mast cells quality-of-life Authors Affiliations Tae Kyeom Kang Korea Institute of Science and Technology View all articles by this author Myungsuk Kim Korea Institute of Science and Technology View all articles by this author Youngsang Yoo Asan Medical Center View all articles by this author Tam Thi Le Korea Institute of Science and Technology View all articles by this author Geon Park Korea Institute of Science and Technology View all articles by this author Yuna Jung Korea Institute of Science and Technology View all articles by this author Hyobin Jeong Yonsei University College of Life Science and Biotechnology View all articles by this author Wook-Bin Lee 0000-0003-4712-5890 [email protected] Korea Institute of Science and Technology View all articles by this author Sang Hoon Jung Korea Institute of Science and Technology View all articles by this author Metrics & Citations Metrics Article Usage 350 views 99 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Tae Kyeom Kang, Myungsuk Kim, Youngsang Yoo, et al. Anti-Allergic Effects of Chamaecrista nomame Extract and Luteolin in an OVA-Induced Asthma Mouse Model. Authorea . 15 January 2025. DOI: https://doi.org/10.22541/au.173697405.55741552/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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