Establishment and Characterization of a Feline Mammary Tumor Patient-Derived Xenograft Model

Abstract BackgroundFeline mammary tumor (FMT) is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies is limited by the shortage of in vivo models that reproduce tumor microenvironment and metastatic progression. ResultsFour FMT-patient‐derived xenografts (PDX) successfully established in NOD.Cg-Prkdcscid IL2rgtm1Wjl/SzJ mice. The overall success rate of PDX establishment was 36% (4/11). Histological, immunohistochemical, and short tandem repeat analysis showed a remarkable similarity between patient's tumor and PDX.ConclusionsIn this study, FMT-PDX model were established. The tumor grafts conserve original tumor essential features, including distant metastasis. FMT-PDX represents an available resource for bridging the biology of FMT with preclinical studies of FMT in cats.