Effect of Huposan on Nitric Oxide and Nitric Oxide Synthase in Endometriosis Rats

Objective: To explain the mechanism of Huposan on Endmetriosis.Methods: To creat EMs Rats of Qi stagnation and blood stasis by auto-transplanting endometrium and tail-clamped.Randomly were divided into five groups: Model group,Gestrinone group,Huposan high dose,Huposan medium dose and Huposan low dose group.All of them were treated respectively for four weeks,and then put them to death.The level of NO and NOS in the serum was detected by colorimetry,and ELISA immunohistochemical method was used to detect the level of PGF2α in the plasma.The morphous of both eutopic and ectopic endometrium was detected by light microscope.Results:①HuPoSan can reduce the level of NO and NOS in the serum of model rats and PGF2α in the plasma of model rats,medium dose team was similar to high dose team,and was lower to the low dose team(P0.01),of which the difference was significant with the model group(P0.01).The difference between low dose team and model group had statistics sign in content of NO(P0.05),was statistics significant in activity of NOS and PGF2α(P0.01).②There were non-statistics significant difference among the group of Gestrinone,medium dose team and high dose team on the level of NO and NOS in the serum and PGF2α in the plasma.(P0.05).③In the group of HuPoSan,the volume of ectopic endometrium was reduced,with glandular organ and inflammatory cell reducing and glandular epithelium atrophy in varying degree,which was similar to the group of Gestrinone and better than the model group.Conclusion: ①HuPoSan can reduce the level of NO and NOS in the serum of model rats,and then reduce the invasion,metastasis and vasiformation process of ectopic endometrium.So we suppose that it may be one of the mechanismses of HuPoSan on EMs.②The circumstance that HuPoSan can reduce the content of PGF2α in the plasma provides theoryetical and experimental foundation that HuPoSan may become a new way of treatment for pain of EMs scientificly.