Changes in biochemical compositions and salinity tolerance responses of different bread wheat cultivated in an arid and semi-arid climate

Changes in biochemical compositions and salinity tolerance responses of different bread wheat cultivated in an arid and semi-arid climate | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Changes in biochemical compositions and salinity tolerance responses of different bread wheat cultivated in an arid and semi-arid climate Saeed Norouzi, Gholamali Akbari This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4172214/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The present study aimed to investigate different responses of some wheat cultivars under salinity conditions. Accordingly, two experiments were performed as Petri and farm studies. Therefore, the effects of different salinity levels (control, 4, 8, and 12 dS.cm − 1 ) were firstly studied on seed germination indices and some growth traits of six bread wheat cultivars (Chamran-2, Mehrgan, Marvdasht, Narin, MS-89-13, and MS-90-13) using a factorial based on the completely randomized design in the Petri experiment for ten days in three replications. Then, responses of the best cultivars selected were compared in both normal and saline farms as combined analysis based on the randomized complete block design during two 2020–2021 and 2021–2022 growing seasons in three replications. The Petri data showed that germination responses against salinity levels were negatively concentration-dependent. The best germination prcentage, T 50 , shoot length, and leaf protein for all cultivars were obtained under the control treatment. Among cultivars, the highest germination percentage, shoot length, and leaf protein and the lowest T 50 of all cultivars were achieved for the Chamran-2 and the minimum values of root length and root length stress tolerance index traits for the MS-89-13 × 12dS.m − 1 salinity interaction. Farm experiment data showed that maximum values for the plant height, 1000-grain weight, grain and biological yields, pigments, grain protein, wet gluten, and gluten index were achieved for plants grown under normal conditions and second year of the experiment. Chamran-2, and then Mehregan, had more proper conditions and had longer plants, heavier grain weight, and higher grain and biological yields. However, the maxium values for wet gluten and gluten index indices were obtained for Mehregan and Narin cultivars, respectively. The highest straw yield was obtained under Chamran-2 cultivar×Normal farm×Second year interaction. The highest catalase activity were recorded for saline conditions and first year of the experiment and the highest superoxide dismutase activity was observed for the Narin cultivar×Saline conditions×Second year interaction. Eventually, considering the predominant characteristics of the farm experiments, Chamran-2 and Mehrgan cultivars can be cultivated in the southern regions of Iran and similar areas as a reference. Biological sciences/Physiology Biological sciences/Plant sciences Earth and environmental sciences/Natural hazards Enzymatic/non-enzymatic activities Gluten index Grain protein Grain yield Salt tolerance Top cultivar Full Text Additional Declarations No competing interests reported. Supplementary Files CATandSODactivities.docx Experimenaldata.docx GerminationindicesPetridata.docx PetridataInteractions.docx Pigments.docx prolinsolublesugarswetglutenglutenindex.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4172214","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":291378351,"identity":"69541443-0259-4038-b99b-a4acb32aa81f","order_by":0,"name":"Saeed 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Accordingly, two experiments were performed as Petri and farm studies. Therefore, the effects of different salinity levels (control, 4, 8, and 12 dS.cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e) were firstly studied on seed germination indices and some growth traits of six bread wheat cultivars (Chamran-2, Mehrgan, Marvdasht, Narin, MS-89-13, and MS-90-13) using a factorial based on the completely randomized design in the Petri experiment for ten days in three replications. Then, responses of the best cultivars selected were compared in both normal and saline farms as combined analysis based on the randomized complete block design during two 2020\u0026ndash;2021 and 2021\u0026ndash;2022 growing seasons in three replications. The Petri data showed that germination responses against salinity levels were negatively concentration-dependent. The best germination prcentage, T\u003csub\u003e50\u003c/sub\u003e, shoot length, and leaf protein for all cultivars were obtained under the control treatment. Among cultivars, the highest germination percentage, shoot length, and leaf protein and the lowest T\u003csub\u003e50\u003c/sub\u003e of all cultivars were achieved for the Chamran-2 and the minimum values of root length and root length stress tolerance index traits for the MS-89-13 \u0026times; 12dS.m\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e salinity interaction. Farm experiment data showed that maximum values for the plant height, 1000-grain weight, grain and biological yields, pigments, grain protein, wet gluten, and gluten index were achieved for plants grown under normal conditions and second year of the experiment. Chamran-2, and then Mehregan, had more proper conditions and had longer plants, heavier grain weight, and higher grain and biological yields. However, the maxium values for wet gluten and gluten index indices were obtained for Mehregan and Narin cultivars, respectively. The highest straw yield was obtained under Chamran-2 cultivar\u0026times;Normal farm\u0026times;Second year interaction. The highest catalase activity were recorded for saline conditions and first year of the experiment and the highest superoxide dismutase activity was observed for the Narin cultivar\u0026times;Saline conditions\u0026times;Second year interaction. Eventually, considering the predominant characteristics of the farm experiments, Chamran-2 and Mehrgan cultivars can be cultivated in the southern regions of Iran and similar areas as a reference.\u003c/p\u003e","manuscriptTitle":"Changes in biochemical compositions and salinity tolerance responses of different bread wheat cultivated in an arid and semi-arid climate","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-04-18 02:47:34","doi":"10.21203/rs.3.rs-4172214/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"ba042953-7df9-42fd-8ce0-3a5d55972c8a","owner":[],"postedDate":"April 18th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":30692174,"name":"Biological sciences/Physiology"},{"id":30692175,"name":"Biological sciences/Plant sciences"},{"id":30692176,"name":"Earth and environmental sciences/Natural hazards"}],"tags":[],"updatedAt":"2025-06-27T14:09:00+00:00","versionOfRecord":[],"versionCreatedAt":"2024-04-18 02:47:34","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4172214","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4172214","identity":"rs-4172214","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}