Genomics of caddisfly (Insecta: Trichoptera) species associated with terrestrial habitats

Genomics of caddisfly (Insecta: Trichoptera) species associated with terrestrial habitats | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Ecology and Evolution This is a preprint and has not been peer reviewed. Data may be preliminary. 14 January 2026 V1 Latest version Share on Genomics of caddisfly (Insecta: Trichoptera) species associated with terrestrial habitats Authors : Jacqueline Heckenhauer 0000-0001-8771-9154 [email protected] , Charlotte Gerheim , Gabriela Jijon , Robert Wisseman , Steffen Pauls , and Paul Frandsen Authors Info & Affiliations https://doi.org/10.22541/au.176843138.85524712/v1 Published Ecology and Evolution Version of record Peer review timeline 147 views 96 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Limnephilidae is a species-rich and ecologically diverse family within the tube case making clade of caddisflies (Trichoptera). Species occur across a wide range of habitats, from cold headwater streams to stagnant pools to terrestrial environments. Here, we sequenced, assembled and annotated whole genomes from two species that are associated with terrestrial environments: Enoicyla pusilla (Burmeister, 1839), distributed in the West Palearctic and Philocasca rivularis (Wiggins, 1968), distributed in the Nearctic. Comprising three species, Enoicyla is the only genus of Trichoptera in Europe that exhibits a completely terrestrial life-cycle. As one of four species of Philocasca, larvae of P. rivularis also exhibit terrestrial behavior during wet months, dispersing across the forest floor up to several meters away from the stream channel. In both species, we investigated genomic features, e.g., genome size and dynamics of transposable elements across Limnephilidae. We also explored potential molecular adaptations of silk to terrestrial versus aquatic environments. Characterisation of the major silk gene, h-fibroin, of both species, as well as elemental analysis of silk of Philocasca rivularis, did not reveal molecular differences compared with silk of aquatic caddisfly species, potentially owing to the humid environments that both species inhabit. The new genomes form key resources for future genomic research on insect evolution, particularly related to habitat diversification and aquatic-terrestrial transitions. Supplementary Material File (appendix_s1_repetitive_dna_content.xlsx) Download 25.11 KB File (appendix_s2_amino_acid_composition_h_fibroin.xlsx) Download 34.12 KB File (maintext.docx) Download 4.48 MB File (supplementary material.pdf) Download 763.90 KB File (table 1.docx) Download 15.16 KB File (table 2.docx) Download 13.43 KB File (table 3.docx) Download 14.21 KB Information & Authors Information Version history V1 Version 1 14 January 2026 Peer review timeline Published Ecology and Evolution Version of Record 1 Apr 2026 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Ecology and Evolution Keywords comparative evolutionary ecology freshwater invertebrate molecular genetics sequencing terrestrial Authors Affiliations Jacqueline Heckenhauer 0000-0001-8771-9154 [email protected] Senckenberg Gesellschaft fur Naturforschung View all articles by this author Charlotte Gerheim Senckenberg Research Institute and Natural History Museum Frankfurt View all articles by this author Gabriela Jijon Brigham Young University View all articles by this author Robert Wisseman Aquatic Biology Associates, Inc. View all articles by this author Steffen Pauls Senckenberg Gesellschaft fur Naturforschung View all articles by this author Paul Frandsen Brigham Young University View all articles by this author Metrics & Citations Metrics Article Usage 147 views 96 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Jacqueline Heckenhauer, Charlotte Gerheim, Gabriela Jijon, et al. Genomics of caddisfly (Insecta: Trichoptera) species associated with terrestrial habitats. Authorea . 14 January 2026. DOI: https://doi.org/10.22541/au.176843138.85524712/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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