Influence of scaffold deformation and fluidmechanical stimuli on bone tissue differentiation

Influence of scaffold deformation and fluidmechanical stimuli on bone tissue differentiation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Influence of scaffold deformation and fluidmechanical stimuli on bone tissue differentiation Laia Moliner-Carrillo, Carlos Ruiz Will, Oscar Camara, Andy L. Olivares This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6363993/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Bone tissue engineering (BTE) offers a promising alternative to graft surgeriesby facilitating bone regeneration through temporary scaffolds that support celladhesion, proliferation, and differentiation. Effective scaffold design is critical, asit must transmit mechanical and fluid stimuli to guide tissue formation. In thisstudy, in silico models were developed to evaluate how various scaffold archi-tectures influence mechanical stimulation and fluid-induced shear stress at thecellular level. Nine scaffold configurations with cylindrical or spherical pores andporosities of 60%, 70%, and 80% were analyzed under static and dynamic com-pression, as well as steady and transient fluid flow. Fluid–structure interaction(FSI) simulations were used to compute octahedral shear strain (SS) and fluidshear stress (FSS), capturing the interplay between scaffold deformation andperfusion. Results indicated that high-porosity scaffolds, particularly the C80configuration, under low compression and slow perfusion, promoted bone differ-entiation while limiting cartilage and fibrous tissue formation. Models neglectingscaffold-fluid coupling overestimated bone formation potential, underscoring theimportance of FSI in replicating physiological conditions. This modeling approachprovides insights for improving BTE strategies and designing more effectivescaffolds for bone repair. Bone tissue engineering mechanobiological modeling fluid shear stress fluid-structure interaction octahedral shear strain scaffold design Full Text Additional Declarations No competing interests reported. Supplementary Files BoneTissueEngineeringsupplementary.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6363993","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":438202932,"identity":"9bf5dae5-6401-4ac1-abf4-bfd992bdec64","order_by":0,"name":"Laia Moliner-Carrillo","email":"","orcid":"","institution":"Pompeu Fabra University","correspondingAuthor":false,"prefix":"","firstName":"Laia","middleName":"","lastName":"Moliner-Carrillo","suffix":""},{"id":438202933,"identity":"5fe82397-34a4-4eaf-8d80-71597dfa83c3","order_by":1,"name":"Carlos Ruiz Will","email":"","orcid":"","institution":"Pompeu Fabra University","correspondingAuthor":false,"prefix":"","firstName":"Carlos","middleName":"Ruiz","lastName":"Will","suffix":""},{"id":438202934,"identity":"206743e2-4a51-4cbe-86d9-d9d0b76f7a5d","order_by":2,"name":"Oscar Camara","email":"","orcid":"","institution":"Pompeu Fabra University","correspondingAuthor":false,"prefix":"","firstName":"Oscar","middleName":"","lastName":"Camara","suffix":""},{"id":438202935,"identity":"f5af193a-200b-418d-91e5-07b61c246eb5","order_by":3,"name":"Andy L. 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