Evaluation of fetal protective effect of Resveratrol against teratogenic effect of sodium valproate in mice through evaluation of inflammation, apoptosis and oxidative stress pathways

Evaluation of fetal protective effect of Resveratrol against teratogenic effect of sodium valproate in mice through evaluation of inflammation, apoptosis and oxidative stress pathways | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Evaluation of fetal protective effect of Resveratrol against teratogenic effect of sodium valproate in mice through evaluation of inflammation, apoptosis and oxidative stress pathways Mojgan Naderi, Abasali Depouri, Noorollah Rezaei, Ramin Ataee This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7868586/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: There are some studies that sodium valproate is associated with some major and minor malformations and its placental transfer influences oxidative stress processes. Resveratrol is a drug with few side effects and high antioxidant and cell protection properties,. Objectives: This study aimed to evaluate the protective effects of Resveratrol as a natural compound with antioxidant properties on oxidative stress and teratogenic effect induced by transfer of transplacental sodium valperoate in mice Methods: The female mice (25-30 gm, 3 weeks years old) were divided into 5 groups (6 mice per group). Of control, Sodium Valperoate (400 mg/kg/day by gavage from day 8 to 18 of pregnancy.) and Resveratrol (225,350,600 mg/kg/day by IP+SodiumValperoate 400mg/kg/day). All groups were received as a single injection. Administration for 10 consecutive days of pregnancy period were done. After baby mice were born, all neonatal mice were killed with beheading. Then, the liver,heart, brain, and kidney tissues were excited. Then samples were used for assessment of oxidative stress markers including lipid peroxidation (LPO), glutathione (GSH), and protein carbonyl (PrC) content .and inflammatory markers as IL6,NfKB,Sirt1 by realtime PCR and their tissues for histopathology studies Results: This study showed that SodiumValperoate caused a significant decrease in GSH concentration. Also were observed a significant increase in lipid peroxidation (LPO) and protein carbonyl contents. However, Resveratrol treatment significantly inhibited oxidative stress markers, decreased IL6, NfKB, and Sirt1 in heart, kidney and liver and brain of neonatal mice significantly which affected by sodium valproate.(P<0.05).Also histopathology studies show protective effect of Resveratrol in theses tissues Conclusions: Our findings showed that resveratrol has a protective effect against oxidative stress and inflammatory process induced by placental transfer of sodium valproate in mice Resveratrol oxidative stress apoptosis bulb C mice Sodium Valproate Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7868586","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":536185602,"identity":"c925880c-986a-4d0d-ac3b-3e3519b63661","order_by":0,"name":"Mojgan Naderi","email":"","orcid":"","institution":"Islamic Azad University","correspondingAuthor":false,"prefix":"","firstName":"Mojgan","middleName":"","lastName":"Naderi","suffix":""},{"id":536185603,"identity":"0390b764-33d7-4a22-9424-aea1ec5a04a1","order_by":1,"name":"Abasali Depouri","email":"","orcid":"","institution":"Islamic Azad 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Resveratrol is a drug with few side effects and high antioxidant and cell protection properties,.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eObjectives:\u003c/strong\u003e This study aimed to evaluate the protective effects of Resveratrol as a natural compound with antioxidant properties on oxidative stress and teratogenic effect induced by transfer of transplacental sodium valperoate in mice\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003e The female mice (25-30 gm, 3 weeks years old) were divided into 5 groups (6 mice per group). Of control, Sodium Valperoate (400 mg/kg/day by gavage from day 8 to 18 of pregnancy.) and Resveratrol (225,350,600 mg/kg/day by IP+SodiumValperoate 400mg/kg/day). All groups were received as a single injection. Administration for 10 consecutive days of pregnancy period were done. After baby mice were born, all neonatal mice were killed with beheading. Then, the liver,heart, brain, and kidney tissues were excited. Then samples were used for assessment of oxidative stress markers including lipid peroxidation (LPO), glutathione (GSH), and protein carbonyl (PrC) content .and inflammatory markers as IL6,NfKB,Sirt1 by realtime PCR and their tissues for histopathology studies\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e This study showed that SodiumValperoate caused a significant decrease in GSH concentration. Also were observed a significant increase in lipid peroxidation (LPO) and protein carbonyl contents. However, Resveratrol treatment significantly inhibited oxidative stress markers, decreased IL6, NfKB, and Sirt1 in heart, kidney and liver and brain of neonatal mice significantly which affected by sodium valproate.(P\u0026lt;0.05).Also histopathology studies show protective effect of Resveratrol in theses tissues\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusions:\u003c/strong\u003e Our findings showed that resveratrol has a protective effect against oxidative stress and inflammatory process induced by placental transfer of sodium valproate in mice\u003c/p\u003e","manuscriptTitle":"Evaluation of fetal protective effect of Resveratrol against teratogenic effect of sodium valproate in mice through evaluation of inflammation, apoptosis and oxidative stress pathways","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-30 06:45:27","doi":"10.21203/rs.3.rs-7868586/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f1616df5-9b72-4a1a-ba60-2d09b4cc614d","owner":[],"postedDate":"October 30th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-11-15T20:38:23+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-30 06:45:27","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7868586","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7868586","identity":"rs-7868586","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}