The effects of 1,25-dihydroxyvitamin D_3 on interstitial fibrosis to kidney after unilateral ureteral obstruction in rats

Objective: To investigate the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] on interstitial fibrosis to kidney after unilateral ureteral obstruction(UUO) in rats.Methods: 45 rats were randomly divided in to 3 groups: sham operated group(n=15) which was given 1,25-(OH)2D3 by intraperitoneal injection one day on post operative every day,UUO group(n=15) and 1,25-(OH)2D3 treatment group(n=15) which were all given normal saline by intraperitioneal injection equivalently.They were sacrificed at the 3rd,7th and 14th days respectively after UUO,every time for only 5.Interstitial fibrosis of obstructed kidney was graded according to HE and Masson staining.The expression of α-SMA was examined by immunohistochemistry.The samples blood were collected to detect the concentration blood serum creatinine and blood urea nitrogen.Daily urinary protein were determined in these rats one day before sacrificed.Results: The degree of interstitial impairment and fibrosis of obstructed kidney and the expression of α-SMA in the UUO group and treatment group were significantly higher than those in the sham operated group(all P0.05).The blood serum creatinine,blood urea nitrogen and daily urinary protein in the UUO group and treatment group were increased at the 3rd and 7th days after UUO,but they were decreased at the 14th day(all P0.05).Compared with UUO group,all which in treatment group were significantly decreased(all P0.05).Conclusion: 1,25-(OH)2D3 can alleviate interstitial fibrosis and renal function in rats after UUO.