Complete Genome Sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-Producing Bacterium Isolated from Benthal Deposits of the Chornobyl NPP Water Cooling Pond

Complete Genome Sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-Producing Bacterium Isolated from Benthal Deposits of the Chornobyl NPP Water Cooling Pond | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Data Note Complete Genome Sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-Producing Bacterium Isolated from Benthal Deposits of the Chornobyl NPP Water Cooling Pond Alina Kharchuk, Maksym Kharchuk, Maksym Kharkhota, Larysa Mozhaieva, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8960927/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract Objectives: Bacillus pseudomycoides , a member of the Bacillus cereus group, is widely distributed in soil and plant-associated environments, where it contributes to ecological interactions through the production of diverse bioactive compounds. Strains of this species are recognized for their capacity to synthesize compounds that enhance stress tolerance and promote overall environmental persistence. In this study, we evaluated the metabolic potential of B. pseudomycoides CHAES I_2_2, isolated from Chornobyl NPP water cooling pond sediments, using whole-genome analysis to characterize radiation resistance determinants and biotechnologically relevant secondary metabolite biosynthetic gene clusters. Data description: We isolated Bacillus pseudomycoides CHAES I_2_2 from benthic sediments of the Chornobyl NPP cooling pond and generated its draft genome sequence. The assembled genome comprises 5,154,520 bp with 35.5% GC content. Genome annotation identified 60 tRNAs, 5 rRNAs, and 4,955 protein-coding sequences. Genome analysis confirmed the presence of a complete polyhydroxyalkanoate biosynthesis gene cluster. The strain possessed genes involved in radiation stress adaptation, including DNA repair, oxidative stress response, sporulation, as well as a paeninodin-like lassopeptide biosynthetic gene cluster and a bacillibactin-type NRPS siderophore cluster. These genomic findings underscore the stress resilience and biotechnological potential of the studied strain. Bacillus pseudomycoides Polyhydroxyalkanoates (PHA) Chornobyl NPP Whole genome sequencing Objective Bacillus pseudomycoides is a Gram-positive, endospore-forming bacterium of the Bacillus cereus group, widely distributed in terrestrial and aquatic environments [1]. B. pseudomycoides strains exhibit substantial metabolic versatility, including antimicrobial peptides [2], biosurfactants [3], and extracellular polymers [4] production. Plant growth-promoting properties of B. pseudomycoides include improving plants’ growth and drought stress tolerance with volatile compounds [5], potassium uptake [6], copper [7] and cadmium [8] removal, antifungal activity [9], Se reduction [10], and salinity stress alleviation [11]. Recently, there were reports on the ability of B. pseudomycoides to synthesize polyhydroxyalkanoates, which is considered a valuable resource for bioplastics production [12, 13]. The Chornobyl Nuclear Power Plant (NPP) cooling pond represents a unique radionuclide-contaminated aquatic ecosystem, characterized by elevated radiation levels and heavy metal contamination. Microbial communities inhabiting this environment play important roles in biogeochemical cycling, environmental adaptation, and ecosystem recovery [14, 15]. We have previously detected spore-forming Bacillaceae family bacteria in the Chornobyl ecosystems [16]. However, despite increasing interest in microbial diversity in the Chornobyl exclusion zone, genomic characterization of individual bacterial isolates remains limited, and to date, no whole-genome sequence of B. pseudomycoides isolated from the Chornobyl cooling pond or exclusion zone has been reported. Here, we report and analyze the draft genome sequence of B. pseudomycoides CHAES I_2_2, isolated in 2017 from the benthal deposits of the Chornobyl NPP cooling pond, Ukraine. To our knowledge, this represents the first whole-genome sequence of B. pseudomycoides obtained from the Chornobyl exclusion zone. Moreover, this is the first report of a polyhydroxyalkanoate-producing B. pseudomycoides strain originating from a radionuclide-contaminated aquatic environment. Data description Table 1 : Overview of data files/data sets. Label Name of data file/data set File types (file extension) Data repository and identifier (DOI or accession number) Data file 1 Circular genomic map of Bacillus pseudomycoides CHAES I 2_2 Portable Document Format file (.pdf) https://doi.org/10.6084/m9.figshare.31354570 [17] Data file 2 Bacillus pseudomycoides CHAES I 2_2 Prokka genome annotation GENBANK file (.gff) https://doi.org/10.6084/m9.figshare.31382134 [18] Data file 3 Bacillus pseudomycoides CHAES I 2_2 COG categories (.jpg) https://doi.org/10.6084/m9.figshare.31382158 [19] Data file 4 Bacillus pseudomycoides CHAES I 2_2 KEGG Pathways (.jpg) https://doi.org/10.6084/m9.figshare.31382182 [20] Data file 5 Bacillus pseudomycoides CHAES I 2_2 EggNOG genome annotation Annotation file (.ANNOTATION) https://doi.org/10.6084/m9.figshare.31382569 [21] Data file 6 Bacillus pseudomycoides CHAES I 2_2 PHA synthetic cluster Portable Document Format file (.pdf) https://doi.org/10.6084/m9.figshare.31382812 [22] Data file 7 Average nucleotide identity analysis results for B. pseudomycoides CHAES I 2_2 .tsv https://doi.org/10.6084/m9.figshare.31382836 [23] Data file 8 Bacillus pseudomycoides CHAES I 2_2 GBDP phylogeny based on 16S data .png https://doi.org/10.6084/m9.figshare.31382872 [24] Data file 9 Bacillus pseudomycoides CHAES I 2_2 GBDP phylogeny based on whole-genome sequence data .png https://doi.org/10.6084/m9.figshare.31382881 [25] Data file 10 Bacillus pseudomycoides CHAES I 2_2 CARD RGI antimicrobial screening results .xlsx https://doi.org/10.6084/m9.figshare.31382902 [26] Data file 11 Bacillus pseudomycoides CHAES I 2_2 virulence factors screening results .csv https://doi.org/10.6084/m9.figshare.31382962 [27] Data file 12 Bacillus pseudomycoides CHAES I 2_2 mobile elements screening results .csv https://doi.org/10.6084/m9.figshare.31402791 [28] Data file 13 Bacillus pseudomycoides CHAES I 2_2 digital DNA-DNA hybridization results .pdf https://doi.org/10.6084/m9.figshare.31382980 [29] Data file 14 Predicted biosynthetic gene clusters of Bacillus pseudomycoides CHAES I 2_2 .pdf https://doi.org/10.6084/m9.figshare.31383001 [30] Data set 1 Genome assembly of B. pseudomycoides CHAES I 2_2 Fasta file (.fna) NCBI Genome Assembly: http://identifiers.org/insdc.gca:GCA_054353755.1 [31] Bacillus pseudomycoides CHAES I 2_2 was isolated from the Chornobyl Nuclear Power Plant (NPP) cooling pond benthic deposit, cultured on agarized Luria–Bertani (LB) medium (24 h, 37 ºC), and deposited in the Ukrainian Collection of Microorganisms (UCM). DNA libraries were prepared using the Quick-DNA Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) and the DNBSEQ Fast PCR-FREE FS Library Prep Set V2.0. Sequencing was performed on the DNBSEQ PE150 platform. Assembly (100× coverage) was generated with Newbler v3.0 and quality-assessed using CheckM v1.2.4 [32]. Genome annotation was conducted using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) v6.10 [33], Prokka v1.15.6 [34], and EggNOG-mapper v2.1.13 [35]. Biosynthetic gene clusters (BGCs), antimicrobial resistance (AMR) genes, virulence factors, and mobile genetic elements (MGEs) were identified using antiSMASH v8.0.4 [36], CARD RGI [37], VirulentHunter [38], and MobileElementFinder v1.1.2 [39], respectively. Average Nucleotide Identity (ANI) was computed using skani within GTDB-Tk v2.6.1 [40], and digital DNA–DNA hybridization (dDDH) was calculated with the Type (Strain) Genome Server (TYGS) [41]. The genome map was visualized using CGView [42]. B. pseudomycoides CHAES I 2_2 genome is 5,154,520 bp with 35.5% GC content [17]. PGAP identified 60 tRNAs, 5 rRNAs, and 4,955 protein-coding sequences (CDSs) [31], while Prokka annotation increased the total CDS number to 5,204 [18]. Of these, 4,247 genes (81.61%) were assigned to COG categories [19] and 4,475 proteins (85,99%) were assigned to KEGG pathways [20]. Analysis revealed a complete polyhydroxyalkanoate (PHA) biosynthesis gene cluster orthologous to the validated cluster of Bacillus cereus YB-4 [22, 43]. Core PHA genes were consistently annotated in PGAP [31], Prokka [18], and EggNOG [21], with functions in precursor supply ( phaJ ), transcriptional regulation ( phaQ ), granule stabilization ( phaP ), acetoacetyl-CoA reduction ( phaB ), and polymer synthesis ( phaC ). Multiple radiation-resistance determinants were present, including genes for homologous recombination, nucleotide excision repair, base excision repair, SOS response, oxidative stress defense, and sporulation regulation. Genome mining identified a paeninodin-like lassopeptide BGC similar to that of Paenibacillus dendritiformis C454 with putative antimicrobial and stress-protective potential [30, 44, 45], and an NRPS-type metallophore cluster closely related to bacillibactin BGCs [30, 46], potentially contributing to radiation tolerance via iron sequestration [47,48]. Genome-based taxonomy assigned the strain to the Bacillus cereus sensu lato group. ANI and dDDH analysis identified Bacillus pseudomycoides DSM 12442 as the closest genome (96.19% ANI; alignment fraction 0.776) [23–25, 29]. AMR screening identified 11 putative resistance genes [26], including tetB(P) (tetracycline resistance); FosB (fosfomycin inactivation); and glycopeptide resistance–related genes ( vanT , vanY , vanW ). Sequence identity to reference AMR proteins (30.3% - 77.5%) with alignment coverage (54.5% - 111.8%) indicated varying degrees of similarity and suggests that some loci may represent divergent homologs rather than fully conserved resistance genes. Virulence factor prediction identified 888 high-confidence candidates, the majority associated with immune modulation, effector delivery systems, adherence, and metabolic or regulatory functions, whereas classical exotoxins and invasion-associated factors were comparatively limited [27]. Mobile elements found in the genome include 3 composite transposons, eleven insertion sequences, and Tn5085 [28], consistent with an environmental Bacillus genome. Limitations In summary, B. pseudomycoides CHAES I_2_2 genome analysis revealed distinct genetic features of this stress-resilient and metabolically competent environmental isolate. However, several limitations should be acknowledged. Functional interpretations of radiation resistance determinants, secondary metabolite biosynthetic gene clusters, antimicrobial resistance loci, and predicted virulence-associated proteins are based primarily on bioinformatic analyses and sequence homology. Experimental validation is required to confirm gene expression, metabolite production, radiation tolerance levels, and the actual phenotypic impact of the identified loci. Future studies integrating transcriptomics, metabolomics, and controlled radiation exposure assays will be essential to substantiate the ecological and biotechnological significance of this strain. Abbreviations AMR — Antimicrobial resistance ANI — Average Nucleotide Identity BGC — Biosynthetic gene cluster CARD RGI — Comprehensive Antibiotic Resistance Database Resistance Gene Identifier CDS — Coding DNA sequence COG — Clusters of Orthologous Groups dDDH — Digital DNA–DNA hybridization GC — Guanine–Cytosine GTDB-Tk — Genome Taxonomy Database Toolkit KEGG — Kyoto Encyclopedia of Genes and Genomes LB — Luria–Bertani MGE — Mobile genetic element NPP — Nuclear Power Plant NRPS — Nonribosomal peptide synthetase PGAP — Prokaryotic Genome Annotation Pipeline PHA — Polyhydroxyalkanoate TYGS — Type (Strain) Genome Server Declarations Ethics approval and consent to participate Not applicable. Consent for publication Not applicable. Availability of data and materials B. pseudomycoides CHAES I_2_2 strain was deposited in the Ukrainian Collection of Microorganisms (UCM). Its complete genome sequence is available on NCBI (genome assembly ASM5435375v1), with corresponding BioSample (accession number SAMN52661584) and BioProject (accession number PRJNA1332261) entries. The data described in this Data note can be freely and openly accessed on Figshare repository (https://figshare.com/). Please see table 1 and references [17-31] for details and links to the data. Competing interests The authors declare no competing interests. Funding This work was supported by the National Academy of Sciences of Ukraine, Grant ID 0125U000677. Authors’ contributions Alina Kharchuk conceptualized and designed the research framework. Maksym Kharkhota, Maksym Kharchuk, and Larysa Mozhaieva conducted laboratory experiments. Alina Kharchuk performed computational genomic analyses. Liliia Avdieieva secured funding for the project. Maksym Kharkhota, Maksym Kharchuk, and Larysa Mozhaieva contributed essential research materials. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8960927","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Data Note","associatedPublications":[],"authors":[{"id":599343563,"identity":"d35ea37b-b4de-48c8-8905-05bac3fdc627","order_by":0,"name":"Alina Kharchuk","email":"data:image/png;base64,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","orcid":"","institution":"D.K. Zabolotny Institute of Microbiology and Virology of the NASU","correspondingAuthor":true,"prefix":"","firstName":"Alina","middleName":"","lastName":"Kharchuk","suffix":""},{"id":599343564,"identity":"f4ca16ca-106d-4ce5-a9c4-d8b35e02b1ca","order_by":1,"name":"Maksym Kharchuk","email":"","orcid":"","institution":"D.K. Zabolotny Institute of Microbiology and Virology of the NASU","correspondingAuthor":false,"prefix":"","firstName":"Maksym","middleName":"","lastName":"Kharchuk","suffix":""},{"id":599343565,"identity":"e2710cdc-1612-405b-b911-e4c503b53396","order_by":2,"name":"Maksym Kharkhota","email":"","orcid":"","institution":"D.K. Zabolotny Institute of Microbiology and Virology of the NASU","correspondingAuthor":false,"prefix":"","firstName":"Maksym","middleName":"","lastName":"Kharkhota","suffix":""},{"id":599343566,"identity":"f84743df-7145-4abd-a491-d2e71b58f25b","order_by":3,"name":"Larysa Mozhaieva","email":"","orcid":"","institution":"D.K. Zabolotny Institute of Microbiology and Virology of the NASU","correspondingAuthor":false,"prefix":"","firstName":"Larysa","middleName":"","lastName":"Mozhaieva","suffix":""},{"id":599343577,"identity":"42774352-2c82-45db-b53d-fe05d2714302","order_by":4,"name":"Liliia Avdieieva","email":"","orcid":"","institution":"D.K. Zabolotny Institute of Microbiology and Virology of the NASU","correspondingAuthor":false,"prefix":"","firstName":"Liliia","middleName":"","lastName":"Avdieieva","suffix":""}],"badges":[],"createdAt":"2026-02-24 20:38:15","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8960927/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8960927/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":104401180,"identity":"b2c8ea77-c6ec-4ffa-82b2-e72fe524f39e","added_by":"auto","created_at":"2026-03-11 12:12:03","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":506543,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8960927/v1/cb99027b-d6ce-4cd1-88f6-52df55e1eb2b.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eComplete Genome Sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-Producing Bacterium Isolated from Benthal Deposits of the Chornobyl NPP Water Cooling Pond\u003c/p\u003e","fulltext":[{"header":"Objective","content":"\u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e is a Gram-positive, endospore-forming bacterium of the \u003cem\u003eBacillus cereus\u003c/em\u003e group, widely distributed in terrestrial and aquatic environments [1]. \u003cem\u003eB. pseudomycoides\u003c/em\u003e strains exhibit substantial metabolic versatility, including antimicrobial peptides [2], biosurfactants [3], and extracellular polymers [4] production. Plant growth-promoting properties of \u003cem\u003eB. pseudomycoides\u003c/em\u003e include improving plants\u0026rsquo; growth and drought stress tolerance with volatile compounds [5], potassium uptake [6], copper [7] and cadmium [8] removal, antifungal activity [9], Se reduction [10], and salinity stress alleviation [11]. Recently, there were reports on the ability of \u003cem\u003eB. pseudomycoides\u0026nbsp;\u003c/em\u003eto synthesize polyhydroxyalkanoates, which is considered a valuable resource for bioplastics production [12, 13].\u003c/p\u003e\n\u003cp\u003eThe Chornobyl Nuclear Power Plant (NPP) cooling pond represents a unique radionuclide-contaminated aquatic ecosystem, characterized by elevated radiation levels and heavy metal contamination. Microbial communities inhabiting this environment play important roles in biogeochemical cycling, environmental adaptation, and ecosystem recovery [14, 15]. \u0026nbsp;We have previously detected spore-forming \u003cem\u003eBacillaceae\u003c/em\u003e family bacteria in the Chornobyl ecosystems [16]. However, despite increasing interest in microbial diversity in the Chornobyl exclusion zone, genomic characterization of individual bacterial isolates remains limited, and to date, no whole-genome sequence of \u003cem\u003eB. pseudomycoides\u003c/em\u003e isolated from the Chornobyl cooling pond or exclusion zone has been reported.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eHere, we report and analyze the draft genome sequence of \u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I_2_2, isolated in 2017 from the benthal deposits of the Chornobyl NPP cooling pond, Ukraine. To our knowledge, this represents the first whole-genome sequence of \u003cem\u003eB. pseudomycoides\u003c/em\u003e obtained from the Chornobyl exclusion zone. Moreover, this is the first report of a polyhydroxyalkanoate-producing \u003cem\u003eB. pseudomycoides\u003c/em\u003e strain originating from a radionuclide-contaminated aquatic environment.\u0026nbsp;\u003c/p\u003e\n"},{"header":"Data description","content":"\u003cp\u003e\u003cstrong\u003eTable 1\u003c/strong\u003e: Overview of data files/data sets.\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"650\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eLabel\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eName of data file/data set\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eFile types\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e(file extension)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eData repository and identifier (DOI or accession number)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eCircular genomic map of \u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ePortable Document Format file (.pdf)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31354570 [17]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 Prokka genome annotation\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eGENBANK file (.gff)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382134 [18]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 COG categories\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e(.jpg)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382158 [19]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 KEGG Pathways\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e(.jpg)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382182 [20]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 EggNOG genome annotation\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eAnnotation file (.ANNOTATION)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382569 [21]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 PHA synthetic cluster\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ePortable Document Format file (.pdf)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382812 [22]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eAverage nucleotide identity analysis results for \u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I 2_2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.tsv\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382836 [23]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 GBDP phylogeny based on 16S data\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.png\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382872 [24]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 GBDP phylogeny based on whole-genome sequence data\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.png\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382881 [25]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 CARD RGI antimicrobial screening results\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.xlsx\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382902 [26]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 virulence factors screening results\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.csv\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382962 [27]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 mobile elements screening results\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.csv\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31402791 [28]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 digital DNA-DNA hybridization results\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.pdf\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31382980 [29]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData file 14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ePredicted biosynthetic gene clusters of\u003cem\u003e\u0026nbsp;Bacillus pseudomycoides\u003c/em\u003e CHAES I 2_2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003e.pdf\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003ehttps://doi.org/10.6084/m9.figshare.31383001 [30]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eData set 1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eGenome assembly of \u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I 2_2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eFasta file (.fna)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\"\u003e\n \u003cp\u003eNCBI Genome Assembly: http://identifiers.org/insdc.gca:GCA_054353755.1\u003c/p\u003e\n \u003cp\u003e[31]\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cem\u003e\u0026nbsp;\u003c/em\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I 2_2 was isolated from the Chornobyl Nuclear Power Plant (NPP) cooling pond benthic deposit, cultured on agarized Luria–Bertani (LB) medium (24 h, 37 ºC), and deposited in the Ukrainian Collection of Microorganisms (UCM). DNA libraries were prepared using the Quick-DNA Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) and the DNBSEQ Fast PCR-FREE FS Library Prep Set V2.0. Sequencing was performed on the DNBSEQ PE150 platform. Assembly (100× coverage) was generated with Newbler v3.0 and quality-assessed using CheckM v1.2.4 [32]. Genome annotation was conducted using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) v6.10 [33], Prokka v1.15.6 [34], and EggNOG-mapper v2.1.13 [35]. Biosynthetic gene clusters (BGCs), antimicrobial resistance (AMR) genes, virulence factors, and mobile genetic elements (MGEs) were identified using antiSMASH v8.0.4 [36], CARD RGI [37], VirulentHunter [38], and MobileElementFinder v1.1.2 [39], respectively. Average Nucleotide Identity (ANI) was computed using skani within GTDB-Tk v2.6.1 [40], and digital DNA–DNA hybridization (dDDH) was calculated with the Type (Strain) Genome Server (TYGS) [41]. The genome map was visualized using CGView [42].\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I 2_2 genome is 5,154,520 bp with 35.5% GC content [17]. PGAP identified 60 tRNAs, 5 rRNAs, and 4,955 protein-coding sequences (CDSs) [31], while Prokka annotation increased the total CDS number to 5,204 [18]. Of these, 4,247 genes (81.61%) were assigned to COG categories [19] and 4,475 proteins (85,99%) were assigned to KEGG pathways [20]. Analysis revealed a complete polyhydroxyalkanoate (PHA) biosynthesis gene cluster orthologous to the validated cluster of \u003cem\u003eBacillus cereus\u003c/em\u003e YB-4 [22, 43]. Core PHA genes were consistently annotated in PGAP [31], Prokka [18], and EggNOG [21], with functions in precursor supply (\u003cem\u003ephaJ\u003c/em\u003e), transcriptional regulation (\u003cem\u003ephaQ\u003c/em\u003e), granule stabilization (\u003cem\u003ephaP\u003c/em\u003e), acetoacetyl-CoA reduction (\u003cem\u003ephaB\u003c/em\u003e), and polymer synthesis (\u003cem\u003ephaC\u003c/em\u003e). Multiple radiation-resistance determinants were present, including genes for homologous recombination, nucleotide excision repair, base excision repair, SOS response, oxidative stress defense, and sporulation regulation. Genome mining identified a paeninodin-like lassopeptide BGC similar to that of \u003cem\u003ePaenibacillus dendritiformis\u003c/em\u003e C454 with putative antimicrobial and stress-protective potential [30, 44, 45], and an NRPS-type metallophore cluster closely related to bacillibactin BGCs [30, 46], potentially contributing to radiation tolerance via iron sequestration [47,48].\u003c/p\u003e\n\u003cp\u003eGenome-based taxonomy assigned the strain to the \u003cem\u003eBacillus cereus\u003c/em\u003e sensu lato group. ANI and dDDH analysis identified \u003cem\u003eBacillus pseudomycoides\u003c/em\u003e DSM 12442 as the closest genome (96.19% ANI; alignment fraction 0.776) [23–25, 29]. AMR screening identified 11 putative resistance genes [26], including \u003cem\u003etetB(P)\u003c/em\u003e (tetracycline resistance); \u003cem\u003eFosB\u003c/em\u003e (fosfomycin inactivation); and glycopeptide resistance–related genes (\u003cem\u003evanT\u003c/em\u003e, \u003cem\u003evanY\u003c/em\u003e, \u003cem\u003evanW\u003c/em\u003e). Sequence identity to reference AMR proteins (30.3% - 77.5%) with alignment coverage (54.5% - 111.8%) indicated varying degrees of similarity and suggests that some loci may represent divergent homologs rather than fully conserved resistance genes. Virulence factor prediction identified 888 high-confidence candidates, the majority associated with immune modulation, effector delivery systems, adherence, and metabolic or regulatory functions, whereas classical exotoxins and invasion-associated factors were comparatively limited [27]. Mobile elements found in the genome include 3 composite transposons, eleven insertion sequences, and Tn5085 [28], consistent with an environmental \u003cem\u003eBacillus\u003c/em\u003e genome.\u003c/p\u003e\n\u003cp\u003eLimitations\u003c/p\u003e\n\u003cp\u003eIn summary, \u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I_2_2 genome analysis revealed distinct genetic features of this stress-resilient and metabolically competent environmental isolate. However, several limitations should be acknowledged. Functional interpretations of radiation resistance determinants, secondary metabolite biosynthetic gene clusters, antimicrobial resistance loci, and predicted virulence-associated proteins are based primarily on bioinformatic analyses and sequence homology. Experimental validation is required to confirm gene expression, metabolite production, radiation tolerance levels, and the actual phenotypic impact of the identified loci. Future studies integrating transcriptomics, metabolomics, and controlled radiation exposure assays will be essential to substantiate the ecological and biotechnological significance of this strain.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eAMR \u0026mdash; Antimicrobial resistance\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;ANI \u0026mdash; Average Nucleotide Identity\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;BGC \u0026mdash; Biosynthetic gene cluster\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;CARD RGI \u0026mdash; Comprehensive Antibiotic Resistance Database Resistance Gene Identifier\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;CDS \u0026mdash; Coding DNA sequence\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;COG \u0026mdash; Clusters of Orthologous Groups\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;dDDH \u0026mdash; Digital DNA\u0026ndash;DNA hybridization\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;GC \u0026mdash; Guanine\u0026ndash;Cytosine\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;GTDB-Tk \u0026mdash; Genome Taxonomy Database Toolkit\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;KEGG \u0026mdash; Kyoto Encyclopedia of Genes and Genomes\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;LB \u0026mdash; Luria\u0026ndash;Bertani\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;MGE \u0026mdash; Mobile genetic element\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;NPP \u0026mdash; Nuclear Power Plant\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;NRPS \u0026mdash; Nonribosomal peptide synthetase\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;PGAP \u0026mdash; Prokaryotic Genome Annotation Pipeline\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;PHA \u0026mdash; Polyhydroxyalkanoate\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;TYGS \u0026mdash; Type (Strain) Genome Server\u003c/p\u003e\n"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003cbr\u003e\u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003cbr\u003e\u003c/strong\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u0026nbsp;\u003cbr\u003e\u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I_2_2 strain was deposited in the Ukrainian Collection of Microorganisms (UCM). Its complete genome sequence is available on NCBI (genome assembly ASM5435375v1), with corresponding BioSample (accession number SAMN52661584) and BioProject (accession number PRJNA1332261) entries.\u003c/p\u003e\n\u003cp\u003eThe data described in this Data note can be freely and openly accessed on Figshare repository (https://figshare.com/). Please see table 1 and references [17-31] for details and links to the data.\u003cem\u003e\u003cbr\u003e\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003cbr\u003e\u003c/strong\u003eThe authors declare no competing interests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003cbr\u003e\u003c/strong\u003eThis work was supported by the National Academy of Sciences of Ukraine, Grant ID 0125U000677.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions\u003cbr\u003e\u003c/strong\u003eAlina Kharchuk conceptualized and designed the research framework. Maksym Kharkhota, Maksym Kharchuk, and Larysa Mozhaieva conducted laboratory experiments. Alina Kharchuk performed computational genomic analyses. Liliia Avdieieva secured funding for the project. Maksym Kharkhota, Maksym Kharchuk, and Larysa Mozhaieva contributed essential research materials. Liliia Avdieieva was responsible for the project administration. Alina Kharchuk prepared the initial manuscript draft. All authors critically reviewed and revised the manuscript for intellectual content.\u003c/p\u003e\n"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eNakamura LK. Bacillus pseudomycoides sp. nov. \u003cem\u003eInt J Syst Bacteriol\u003c/em\u003e. 1998;48(3):1031\u0026ndash;1035. https://doi.org/10.1099/00207713-48-3-1031\u003c/li\u003e\n\u003cli\u003eBasi-Chipalu S, Dischinger J, Josten M, Szekat C, Zweynert A, Sahl HG, et al. 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Insights into the unique phosphorylation of the lasso peptide paeninodin. \u003cem\u003eJ Biol Chem\u003c/em\u003e. 2016;291(26):13662\u0026ndash;13678. https://doi.org/10.1074/jbc.m116.722108\u003c/li\u003e\n\u003cli\u003eHegemann JD, Zimmermann M, Xie X, Marahiel MA. Lasso peptides: an intriguing class of bacterial natural products. \u003cem\u003eAcc Chem Res\u003c/em\u003e. 2015;48(7):1909\u0026ndash;1919. https://doi.org/10.1021/acs.accounts.5b00156\u003c/li\u003e\n\u003cli\u003eWu Q, Throckmorton K, Maity M, Chevrette MG, Braun DR, Rajski SR, et al. Bacillibactins E and F from a marine sponge-associated \u003cem\u003eBacillus\u003c/em\u003e sp. \u003cem\u003eJ Nat Prod\u003c/em\u003e. 2020;84(1):136\u0026ndash;141. https://doi.org/10.1021/acs.jnatprod.0c01170\u003c/li\u003e\n\u003cli\u003eDaly MJ. A new perspective on radiation resistance based on \u003cem\u003eDeinococcus radiodurans\u003c/em\u003e. \u003cem\u003eNat Rev Microbiol\u003c/em\u003e. 2009;7(3):237\u0026ndash;245. https://doi.org/10.1038/nrmicro2073\u003c/li\u003e\n\u003cli\u003eMiethke M, Marahiel MA. Siderophore-based iron acquisition and pathogen control. \u003cem\u003eMicrobiol Mol Biol Rev\u003c/em\u003e. 2007;71(3):413\u0026ndash;451. https://doi.org/10.1128/MMBR.00012-07\u003c/li\u003e\n\u003c/ol\u003e\n\n"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-genomic-data","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gtic","sideBox":"Learn more about [BMC Genomic Data](http://bmcgenet.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/gtic/default.aspx","title":"BMC Genomic Data","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Bacillus pseudomycoides, Polyhydroxyalkanoates (PHA), Chornobyl NPP, Whole genome sequencing","lastPublishedDoi":"10.21203/rs.3.rs-8960927/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8960927/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eObjectives:\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eBacillus pseudomycoides\u003c/em\u003e, a member of the \u003cem\u003eBacillus cereus\u003c/em\u003egroup, is widely distributed in soil and plant-associated environments, where it contributes to ecological interactions through the production of diverse bioactive compounds. Strains of this species are recognized for their capacity to synthesize compounds that enhance stress tolerance and promote overall environmental persistence. In this study, we evaluated the metabolic potential of \u003cem\u003eB. pseudomycoides\u003c/em\u003e CHAES I_2_2, isolated from Chornobyl NPP water cooling pond sediments, using whole-genome analysis to characterize radiation resistance determinants and biotechnologically relevant secondary metabolite biosynthetic gene clusters.\u003c/p\u003e\n\u003cp\u003eData description:\u003c/p\u003e\n\u003cp\u003eWe isolated \u003cem\u003eBacillus pseudomycoides\u003c/em\u003e CHAES I_2_2 from benthic sediments of the Chornobyl NPP cooling pond and generated its draft genome sequence. The assembled genome comprises 5,154,520 bp with 35.5% GC content. Genome annotation identified 60 tRNAs, 5 rRNAs, and 4,955 protein-coding sequences. Genome analysis confirmed the presence of a complete polyhydroxyalkanoate biosynthesis gene cluster. The strain possessed genes involved in radiation stress adaptation, including DNA repair, oxidative stress response, sporulation, as well as a paeninodin-like lassopeptide biosynthetic gene cluster and a bacillibactin-type NRPS siderophore cluster. These genomic findings underscore the stress resilience and biotechnological potential of the studied strain.\u003c/p\u003e","manuscriptTitle":"Complete Genome Sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-Producing Bacterium Isolated from Benthal Deposits of the Chornobyl NPP Water Cooling Pond","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-03-03 19:12:17","doi":"10.21203/rs.3.rs-8960927/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-03-30T16:24:19+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-14T16:10:49+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-09T21:16:16+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-02T13:18:49+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"36398391589869338990497233400431490784","date":"2026-03-02T12:10:47+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"236640612894016753784635499471569068151","date":"2026-02-28T17:56:11+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"4578937795659004294755040598072829156","date":"2026-02-27T16:25:12+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"3552628533081249378267961537141131522","date":"2026-02-27T13:34:52+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-02-26T17:50:44+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2026-02-25T17:54:14+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2026-02-25T17:50:29+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Genomic Data","date":"2026-02-24T20:28:41+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-genomic-data","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"gtic","sideBox":"Learn more about [BMC Genomic Data](http://bmcgenet.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/gtic/default.aspx","title":"BMC Genomic Data","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"6e594d17-7282-4e0a-8966-d52af209bad7","owner":[],"postedDate":"March 3rd, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2026-04-29T09:10:29+00:00","versionOfRecord":[],"versionCreatedAt":"2026-03-03 19:12:17","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8960927","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8960927","identity":"rs-8960927","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}