The modulation of leaf cell-wall composition in Arabidopsis thaliana by the drought-mitigating rhizobacterium, Bacillus endophyticus J13

The modulation of leaf cell-wall composition in Arabidopsis thaliana by the drought-mitigating rhizobacterium, Bacillus endophyticus J13 | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 15 May 2025 V1 Latest version Share on The modulation of leaf cell-wall composition in Arabidopsis thaliana by the drought-mitigating rhizobacterium, Bacillus endophyticus J13 Authors : Raunak Sharma , Lavi Rastogi , Prashant Anupama-Mohan Pawar , Nikhil P.T. , and Sridev Mohapatra 0000-0002-8462-6902 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174731280.00511966/v1 179 views 142 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Plant growth-promoting rhizobacteria (PGPR) are beneficial soil bacteria that reside near plant roots (in the rhizosphere) and support plants in various ways. The specific molecular mechanisms involved in these beneficial interactions are still under scrutiny. In this context, the present study describes the role of Bacillus endophyticus J13, a multiple abiotic-stress tolerant PGPR, in modulating various components of the leaf cell wall, in Arabidopsis thaliana , under well-watered and drought conditions. We have previously reported the positive impact of J13 on drought mitigation in A. thaliana [(Sharma et al., 2024)](#ref-0030) by modulation of soil water content. In the present study, we inoculated A. thaliana roots with J13, under well-watered or water-stressed conditions and analyzed the cell wall composition in leaves under the different treatments. We observed an inoculation specific reduction in lignin content, both under well-watered and water stressed conditions, while the cellulose content was found to be significantly high in water-stressed plants inoculated with J13. The expression of genes involved in, both, the lignin as well as cellulose biosynthetic pathways were upregulated in water-stressed plants, inoculated with J13. J13 inoculation in A. thaliana mutants, compromised in cellulose biosynthesis, eliminated the beneficial impact of the bacteria, indicating the imperativeness of a functional cellulose biosynthetic pathway in J13-mediated drought-tolerance in A. thaliana . J13 inoculation in A. thaliana mutants, with altered lignin composition (mutants with higher S lignin), exhibits no beneficial impact by J13, demonstrating importance of lignin composition towards plant-growth promoting activity of J13 in A. thaliana. This study demonstrates that J13 enhances the saccharification efficiency of A. thaliana leaves, under dehydration stress, by reducing the amount of lignin and elevating the amount of cellulose. Overall, our study suggested that modulation in cell-wall composition could be an important mechanism employed by J13 for drought mitigation in A. thaliana also positively influences polysaccharide digestibility. Therefore, such a strategy may be used to improve plant immunity and lignocellulosic properties. Supplementary Material File (manuscipt_sharma et al.docx) Download 17.80 MB Information & Authors Information Version history V1 Version 1 15 May 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords abiotic stress biofuels development growth plant cell wall plant growth-promoting rhizobacteria (pgpr) Authors Affiliations Raunak Sharma BITS Pilani - Hyderabad Campus View all articles by this author Lavi Rastogi Regional Centre for Biotechnology View all articles by this author Prashant Anupama-Mohan Pawar Regional Centre for Biotechnology View all articles by this author Nikhil P.T. BITS Pilani - Hyderabad Campus View all articles by this author Sridev Mohapatra 0000-0002-8462-6902 [email protected] BITS Pilani - Hyderabad Campus View all articles by this author Metrics & Citations Metrics Article Usage 179 views 142 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Raunak Sharma, Lavi Rastogi, Prashant Anupama-Mohan Pawar, et al. The modulation of leaf cell-wall composition in Arabidopsis thaliana by the drought-mitigating rhizobacterium, Bacillus endophyticus J13. Authorea . 15 May 2025. DOI: https://doi.org/10.22541/au.174731280.00511966/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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