Wavelets Synchronous Spectrofluorimetric Determination of Remdesivir in the Presence of Its Alkaline-Induced Degradation Product

Wavelets Synchronous Spectrofluorimetric Determination of Remdesivir in the Presence of Its Alkaline-Induced Degradation Product | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Wavelets Synchronous Spectrofluorimetric Determination of Remdesivir in the Presence of Its Alkaline-Induced Degradation Product Hamed Abu Saeda, Ahmed H. Abdel-Monem, Abdul Aziz M. El-Attar, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8998093/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 10 You are reading this latest preprint version Abstract Fluorescence spectroscopy has lately emerged as a very promising analytical technique due to its exceptional sensitivity and environmentally friendly attributes. In pharmaceutical quality control, there is an increasing demand for analytical methods that are straightforward, swift, cost-effective, and able to deliver dependable results with high sample throughput. Remdesivir is a novel antiviral drug first formulated for the treatment of Ebola virus sickness. In 2020, the United States Food and Drug Administration (US-FDA) approved it for the treatment of COVID-19 infections caused by the SARS-CoV-2 virus. The primary objective of this study is to develop a sensitive, accurate, and exact wavelet synchronous spectrofluorimetric approach for the quantification of Remdesivir in bulk powder and pharmaceutical formulations, accounting for its alkaline-induced degradation product. The emission was documented at 248 nm following stimulation at 408 nm. The enhanced emission exhibited a strong linear association between wavelet synchronous fluorescence intensity and Remdesivir concentration, ranging from 100 to 1200 ng/ml, with a determination coefficient of 0.9997. The proposed approach was validated in accordance with ICH principles and was effectively utilized for the quantification of Remdesivir in bulk powder and pharmaceutical formulations, despite the presence of its alkaline-induced breakdown product. Biological sciences/Biological techniques Biological sciences/Biotechnology Physical sciences/Chemistry Biological sciences/Drug discovery wavelets synchronous Synchronous fluorimetry pharmaceutical preparations Alkaline-induced degradation product Remdesivir Full Text Additional Declarations No competing interests reported. Supplementary Files supplementryfile.docx Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 06 May, 2026 Reviews received at journal 30 Mar, 2026 Reviewers agreed at journal 30 Mar, 2026 Reviews received at journal 27 Mar, 2026 Reviewers agreed at journal 27 Mar, 2026 Reviewers invited by journal 10 Mar, 2026 Editor assigned by journal 10 Mar, 2026 Editor invited by journal 10 Mar, 2026 Submission checks completed at journal 06 Mar, 2026 First submitted to journal 06 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8998093","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":604830288,"identity":"3ebde5c8-3242-4eed-a863-3684684f3fbc","order_by":0,"name":"Hamed Abu Saeda","email":"","orcid":"","institution":"Al Azhar University","correspondingAuthor":false,"prefix":"","firstName":"Hamed","middleName":"Abu","lastName":"Saeda","suffix":""},{"id":604830289,"identity":"f8c02b11-3e75-435a-9f84-af5c50f76274","order_by":1,"name":"Ahmed H. Abdel-Monem","email":"","orcid":"","institution":"Al Azhar University","correspondingAuthor":false,"prefix":"","firstName":"Ahmed","middleName":"H.","lastName":"Abdel-Monem","suffix":""},{"id":604830291,"identity":"5c861d6a-9802-432c-a38f-e28553982360","order_by":2,"name":"Abdul Aziz M. El-Attar","email":"","orcid":"","institution":"Al Azhar University","correspondingAuthor":false,"prefix":"","firstName":"Abdul","middleName":"Aziz M.","lastName":"El-Attar","suffix":""},{"id":604830294,"identity":"9688066b-11a4-497e-970f-f1d059d9b5bd","order_by":3,"name":"Mostafa M Abd Elreheem","email":"data:image/png;base64,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","orcid":"","institution":"Al Azhar University","correspondingAuthor":true,"prefix":"","firstName":"Mostafa","middleName":"M Abd","lastName":"Elreheem","suffix":""}],"badges":[],"createdAt":"2026-02-28 22:08:11","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8998093/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8998093/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":104562151,"identity":"7a4631e6-bbc3-485a-bcc9-1ba8981bc9e3","added_by":"auto","created_at":"2026-03-13 10:35:48","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":890469,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8998093/v1_covered_d6e7c8ab-2b24-4010-beb8-5f6f997610f2.pdf"},{"id":104562127,"identity":"35a859ec-b97b-4cad-aa8e-d189978969eb","added_by":"auto","created_at":"2026-03-13 10:35:42","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":881592,"visible":true,"origin":"","legend":"","description":"","filename":"supplementryfile.docx","url":"https://assets-eu.researchsquare.com/files/rs-8998093/v1/0d0b265c49a40e93d6194a70.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Wavelets Synchronous Spectrofluorimetric Determination of Remdesivir in the Presence of Its Alkaline-Induced Degradation Product","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"wavelets synchronous, Synchronous fluorimetry, pharmaceutical preparations, Alkaline-induced degradation product, Remdesivir","lastPublishedDoi":"10.21203/rs.3.rs-8998093/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8998093/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eFluorescence spectroscopy has lately emerged as a very promising analytical technique due to its exceptional sensitivity and environmentally friendly attributes. In pharmaceutical quality control, there is an increasing demand for analytical methods that are straightforward, swift, cost-effective, and able to deliver dependable results with high sample throughput. Remdesivir is a novel antiviral drug first formulated for the treatment of Ebola virus sickness. In 2020, the United States Food and Drug Administration (US-FDA) approved it for the treatment of COVID-19 infections caused by the SARS-CoV-2 virus. The primary objective of this study is to develop a sensitive, accurate, and exact wavelet synchronous spectrofluorimetric approach for the quantification of Remdesivir in bulk powder and pharmaceutical formulations, accounting for its alkaline-induced degradation product. The emission was documented at 248 nm following stimulation at 408 nm. The enhanced emission exhibited a strong linear association between wavelet synchronous fluorescence intensity and Remdesivir concentration, ranging from 100 to 1200 ng/ml, with a determination coefficient of 0.9997. The proposed approach was validated in accordance with ICH principles and was effectively utilized for the quantification of Remdesivir in bulk powder and pharmaceutical formulations, despite the presence of its alkaline-induced breakdown product.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e","manuscriptTitle":"Wavelets Synchronous Spectrofluorimetric Determination of Remdesivir in the Presence of Its Alkaline-Induced Degradation Product","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-03-13 10:35:35","doi":"10.21203/rs.3.rs-8998093/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"165110482028770370310708414169357484192","date":"2026-05-06T06:00:48+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-30T09:43:30+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"287307998383199598074313688257873644917","date":"2026-03-30T08:56:42+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-03-27T10:34:55+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"45568131346815452774754514123579426594","date":"2026-03-27T05:07:08+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-03-10T05:45:51+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2026-03-10T05:40:42+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2026-03-10T05:03:23+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2026-03-06T13:52:28+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2026-03-06T12:10:45+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"0a2103ff-54d1-44ea-b333-5ded71528089","owner":[],"postedDate":"March 13th, 2026","published":true,"recentEditorialEvents":[{"type":"reviewerAgreed","content":"165110482028770370310708414169357484192","date":"2026-05-06T06:00:48+00:00","index":58,"fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[{"id":64365889,"name":"Biological sciences/Biological techniques"},{"id":64365890,"name":"Biological sciences/Biotechnology"},{"id":64365891,"name":"Physical sciences/Chemistry"},{"id":64365892,"name":"Biological sciences/Drug discovery"}],"tags":[],"updatedAt":"2026-03-13T10:35:36+00:00","versionOfRecord":[],"versionCreatedAt":"2026-03-13 10:35:35","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8998093","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8998093","identity":"rs-8998093","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}