An iridium(III) complex based luminogenic probe for high-throughput screening of H2S donors in living cells

An iridium(III) complex based luminogenic probe for high-throughput screening of H2S donors in living cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article An iridium(III) complex based luminogenic probe for high-throughput screening of H2S donors in living cells Ke-Jia Wu, Wen Sun, Jian-Min Sun, Chang Lu, Yan Li, Ning Sun, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4425986/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 13 Nov, 2024 Read the published version in Communications Chemistry → Version 1 posted You are reading this latest preprint version Abstract The scarcity of suitable high-throughput screening technology for H 2 S donors has hampered the discovery of H 2 S donors. In this study, a long-lived cyclometalated iridium complex was rationally designed as a mitochondria-targeted H 2 S probe to monitor the real-time dynamic change of H 2 S. By using time-resolved emission spectroscopy (TRES) technique, an anti-interference high-throughput screening system was developed to monitor H 2 S in living cells with decreased false negative results. As a proof-of-concept, three natural products were identified as potential H 2 S donors from a natural product library using the developed TRES probe. Notably, the discovery of allicin and diallyl trisulfide demonstrated the feasibility of this screening platform, while garlic derived allyl methyl sulfide was explored as a H 2 S donor candidate. The results were further validated by a commercial assay. We anticipate this high-throughput platform could facilitate the discovery of H 2 S donors by discriminating the endogenous interfering fluorescence from biological system. Biological sciences/Drug discovery Biological sciences/Chemical biology Iridium(III) complex Time-resolved emission spectroscopy (TRES) High-throughput screening H2S donor Anti-interference Full Text Additional Declarations There is NO Competing Interest. Supplementary Files H2SESIfinal.docx Cite Share Download PDF Status: Published Journal Publication published 13 Nov, 2024 Read the published version in Communications Chemistry → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4425986","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":305494365,"identity":"02450c98-3457-4191-b428-a0a74fb5e209","order_by":0,"name":"Ke-Jia 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