Molecular characterization of Tetragonia tetragonoides (Pall.) aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress

Molecular characterization of Tetragonia tetragonoides (Pall.) aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Molecular characterization of Tetragonia tetragonoides (Pall.) aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress Lisha Cao, Fuying Xie, Lihua Chen, Zhengfeng Wang, Mei Zhang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7136705/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 31 Oct, 2025 Read the published version in BMC Plant Biology → Version 1 posted 11 You are reading this latest preprint version Abstract Tetragonia tetragonoides (Pall.) Kuntze (Aizoaceae, 2n = 2x = 32) is a secretohalophyte with excellent tolerance to high salinity–alkalinity, drought, and heat stress. As a medicinal and edible vegetable, T. tetragonoides is widely distributed in the coastal tropics and subtropics worldwide. Aquaporin (AQP) proteins, belonging to the major intrinsic protein (MIP) superfamily, are water channel proteins that facilitate the transport of water and other substrates across cell membranes. AQPs play important roles in physiological processes, including water transport, nutrient acquisition (carbon, nitrogen, and micronutrients), and abiotic stress responses. However, knowledge of AQPs in the special habitat plant T. tetragonoides is limited. In this study, we identified 58 candidate TtAQP genes in the T. tetragonoides genome and classified them into five subfamilies based on phylogenetic analysis with 15 plasma membrane intrinsic proteins (PIPs), 18 tonoplast intrinsic proteins (TIPs), 19 NOD26-like intrinsic proteins (NIPs), 4 small basic intrinsic proteins (SIPs) and 2 uncategorized X intrinsic proteins (XIPs). Gene structure and protein conserved domain analyses showed that the majority of the deduced TtAQPs contained signature transmembrane domains, NPA motifs, the ar/R selectivity filter, and Froger’s positions, suggesting substrate specificity for these TtAQPs. Analyses of cis -acting elements (CEs) in TtAQP s’ promoter regions revealed the presence of stress-responsive and hormone responsive elements, indicating complex regulatory mechanisms for the expression of TtAQP s. TtAQP s exhibited different expression patterns among tissues and under different stress conditions based on RNA sequencing and quantitative reverse transcription PCR assays. Several TtAQP s were cloned and heterologously expressed in yeast to confirm the stress tolerance conferred by the overexpression of these genes. Our findings provide a comprehensive framework for further functional studies of TtAQP s and their potential applications in crop genetic improvement. The results also enhance our understanding of the ecological adaptability of T. tetragonoides to extremely harsh environments and offer valuable insights for developing stress-tolerant transgenic plants through genetic engineering techniques. Aquaporins (AQPs) Abiotic stress Ecological adaptability Tetragonia tetragonoides Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Background Tetragonia tetragonoides (Pall.) Kuntze is a calcium-rich vegetable with substantial commercial value for human health. T. tetragonoides is widely distributed in the coastal areas of tropical and subtropical regions, and it has gradually emerged as a novel coastal cash crop both for the farm economy and for the improvement of coastal saline soils [ 1 , 2 ]. This species is adaptable to multiple extreme stressors, including heat, drought, sea waterlogging, and high salinity–alkalinity, and it grows widely in the tropical coastal zones, including beach sand, mangrove fringe, islands, and reefs [ 2 ]. The suitable salinity range for regular growth of T. tetragonoides is below 2.5% (NaCl); thus, its life cycle can also be completed normally in seawater irrigation areas, which means that this species could be developed as a potential plant for perennial saltwater intrusion areas [ 3 ]. Furthermore, T. tetragonoides has a high nutritional value and economic value due to its high content of inorganic salts (such as calcium (Ca), sodium (Na), and potassium (K)) and several bioactive compounds, including cerebrosides, steryl glucosides, diterpenes, flavonol glycosides, and lignan amides, which make it a feature seawater vegetable with wide cultivation [ 3 , 4 ]. In recent years, saltwater intrusion has constrained the development of marine agriculture [ 5 ]. Therefore, the exploration and promotion of salt-tolerant crops play an important role in marine agriculture development [ 6 ]. T. tetragonioides is an annual or biennial endo-secretohalophyte with salt bladders in the epidermal cells of its leaves and stems; thus, this species can be used as a salt-removing plant due to its high Na + and K uptake efficiency [ 7 ]. Considering this, T. tetragonioides can withstand an electrical conductivity (EC) in the growing medium as high as 10 dS m − 1 (about 100 mM NaCl) and can complete its life cycle normally [ 7 , 8 ]. Some reports have indicated that salinity levels of 50–100 mM NaCl (EC 5–10 dS m − 1 ) can result in a salt-induced growth response in T. tetragonioides [ 9 ]. In its native habitats, the increased water use efficiency (WUE) in T. tetragonioides caused by seawater irrigation is a key influencing factor for its growth and development [ 1 ], which results in high hydraulic conductivity, which likely involves aquaporins (AQPs), similar to another model halophyte species, Eutrema salsugineum [ 10 ]. The related osmotic stress caused by multiple stress substances and environmental challenges in vivo and in vitro must be relieved by osmosensors, and therefore, AQPs must act as master regulators of the osmotic stress response [ 11 ]. AQPs are also called water channel proteins, belonging to the major intrinsic protein (MIP) superfamily and being small membrane proteins (21–34 kD). AQPs mainly function in regulating cellular water transport [ 12 , 13 ]. Plant AQPs can be subdivided into five major groups based on their different subcellular or plant organ localizations: plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs; which target the endoplasmic reticulum), and uncategorized X intrinsic proteins (XIPs) [ 14 ]. The PIP subfamily can be further subdivided into two groups: PIP1and PIP2 [ 15 ]. Plant PIPs present dual localization in the plasma membrane (PM) and chloroplast envelope [ 13 , 15 ]. In addition to water molecules, AQPs also facilitate the transport of H 2 O 2 , gasotransmitters (including NO), H 2 S, ammonia (NH 3 ), carbon dioxide (CO 2 ), and other uncharged solutes across cell membranes [ 16 ], but some studies have demonstrated that plant AQPs (mainly NIPs) also facilitate the diffusion of boron, silicon, and some trivalent (3+) and quadrivalent (4+) elements and compounds across membranes [ 17 – 21 ]. Abiotic stress has become a great challenge for plant growth and distribution due to climate change and environmental degradation, threatening food security and human survival and reproduction [ 22 ]. To date, many studies have evaluated the regulating roles of AQPs involved in abiotic stress responses through their normal roles as water channel proteins and in the transport of other small solute molecules [ 15 , 16 , 22 ]. Among them, drought and high salinity/alkalinity are two major threatening factors that have a serious effect on plants living in special habitats, such as desert areas and coastal saline regions [ 23 , 24 ]. AQPs facilitate efficient transport of water molecules across membranes in almost all higher organisms in nature and, thus, they have been the focus of research decoding environmental challenges in plant genetics and crop improvement [ 25 , 26 ]. For example, transgenic Arabidopsis and tobacco plants overexpressing AtPIP1;4 or AtPIP2;5 showed enhanced tolerance to salt, drought, and cold stress [ 27 ]. GmPIP1;6 overexpression in soybean conferred enhanced salt tolerance and increased yield, mainly due to increased seed size [ 28 ]. MusaPIP1;2 and MusaPIP2;6 overexpression in banana resulted in better abiotic stress survival characteristics compared to non-transformed control plants [ 29 , 30 ]. Similar results have also been presented in barley [ 31 ], tomato [ 32 ], apple [ 33 ], citrus [ 34 ], wheat [ 35 , 36 ], and many special habitat plants. However, some overexpression assays for individual AQP s have led to decreased tolerance to abiotic stress. For example, ectopic overexpression of Glycine soja GsTIP2;1 [ 37 ] and GsPIP2;1 [ 38 ] in Arabidopsis thaliana resulted in sensitivities to salt and dehydration stress. In our previous study, overexpression of Canavalia rosea CrPIP1;5 in Arabidopsis caused weak sensitivity to drought treatment [ 40 ], while CrPIP2;3 overexpression increased resistance to drought in transgenic Arabidopsis [ 41 ]. CrPIP1;5 and CrPIP2;3 proteins interact, likely forming a heterotetramer water channel and performing a water transport function across the cell membrane [ 40 , 41 ]. Both the tolerance and sensitivity to drought or salt stress hinge on the water transport efficiency mediated by specific plant AQPs. The different sites or pathways of water transport (from outside into plant or from the plant to its surroundings) might present the exact opposite effects in transgenic plant phenotypes, which further depends on the localization or tissue-specific distribution of the ectopic accumulation of transgenic AQPs and the protein complex interacting with the AQPs, acting as functional modules. In recent years, molecular biology research on special habitat plants has focused on their mechanisms or pathways for ecological flexibility and adaptability exploration. A meaningful research focus is the halophyte AQP families due to their effects on a range of physiological processes, including water-deficit and high-osmotic stress [ 42 ]. Eutrema salsugineum , an extremophile model species for studying salt resistance, is closely related to A. thaliana . There are 35 EsAQP s in the E. salsugineum genome, and their expression is responsive to salt, drought, and cold stress [ 43 ]. EsPIP1;2 and EsPIP1;4 overexpression in Arabidopsis resulted in a salt stress-sensitive phenotype [ 10 ]. Olive trees ( Olea europaea L.) [ 44 ] and jujube ( Ziziphus jujuba Mill.) [ 45 ] are both drought-resistant plants, and their AQP families have been systematically characterized based on the structure and functional or evolution diversity of different subfamilies. Pearl millet ( Pennisetum glaucum ) is a cereal grown for food, grain, and straw in arid and semi-arid regions. Based on its genome sequencing information, the PgAQP family in pearl millet has been characterized using a genomic approach, and functional expression analyses have been performed [ 46 ]. Acacia auriculiformis is a commercially important forest tree with strong drought tolerance and 21 AaAQP genes, and functional prediction has been performed based on bioinformatics analysis and gene expression profiles. AaPIP1-2 overexpression in Arabidopsis promotes tolerance to drought stress, further providing experimental evidence for the regulation of abiotic stress responses by AQP s [ 47 ]. Goji ( Lycium barbarum ) is an important food and medical plant that adapts well to strong light, arid, and saline environments. A total of 38 LbAQP s have been identified and characterized through systemic bioinformatics and expression analyses, and their possible roles in abiotic stress responses have been further studied through transcript analysis [ 48 ]. Mangrove plants Kandelia obovata and Avicennia marina both present excellent adaptability to extreme environments, such as high salinity and tidal inundation, and the KoAQP (34 members) and AmAQP families (46 members) have been systematically identified, further demonstrating their possible environmental adaptation [ 49 , 50 ]. Woody plant Populus euphratica possesses excellent drought and salt tolerance, and it is often used as a model forest tree in arid regions. The PeuAQP gene family is involved in the tolerance and responses to different abiotic stressors [ 51 ]. Based on released plant genome sequencing information, gene family identification is a simple method for exploring molecular mechanisms and gene functions in plant molecular biology. Tetragonia tetragonioides , also called New Zealand spinach and French spinach, is a halophyte adapted to environments with high salinity and temperature extremes, especially arid and semi-arid areas. The native habitat and planting areas of T. tetragonioides often have high saline/alkaline paddy soil and are threatened by unpredictable seawater flow, thereby causing destruction of the ecological environment and economic losses for farmers. Determining the possible mechanisms by which T. tetragonioides responds to salinity/alkalinity and drought/high osmotic stress is of critical importance. As an atypical succulent plant, the abundant water storage in the aboveground parts of T. tetragonioides is the key factor for its development, enabling survival and adaptation in its natural habitat. Water absorption, storage, and redistribution in plants in vivo depend critically on the specific functions of multiple AQPs. Through the functional analyses of this extremophile’s TtAQP s, our research paves the way for a better understanding of the roles of TtAQP s, the stress tolerance mechanism, and the ecological adaptability of this halophyte. In this study, we searched the T. tetragonioides genome database for all TtAQP members and conducted TtAQP family-based association studies. Their expression profiles were systematically evaluated to elucidate the association of TtAQP s with the molecular mechanism of ecological adaptation. As a result, 58 TtAQP genes were identified and further phylogenetic analysis, protein structure prediction, cis -acting element (CE) analysis for promoters, and yeast heterologous function validation were explored. The results of our study also offer a foundation and genetic resources for further plant breeding of species with high stress tolerance, especially those that can survive and breed in areas that are arid and have seawater flow. Materials and methods Plant materials, growth conditions, and stress treatments Tetragonia tetragonoides seeds were germinated, and the seedlings or adult plants were cultivated as previously described [ 52 ]. The four stress treatments for T. tetragonoides seedlings were set as follows: heat (45℃), high osmotic stress (mimicking drought stress, 300 mM mannitol), high salinity (600 mM NaCl), and high alkalinity (150 mM NaHCO 3 , pH 8.2). Briefly, the T. tetragonoides seedlings were moved to the 45℃ illumination incubator from a greenhouse (22–26℃) for different periods to simulate the heat treatment. The seedlings were removed from their pots, which contained vermiculite, and carefully cleaned. They were then transferred, with their roots submerged into solutions to perform the other three stress treatments. Half-strength Murashige and Skoog (1/2 MS) liquid medium was the basal solution for preparing the treatment solutions. Seedlings treated with standard 1/2 MS liquid medium were set as controls. Database search for TtAQP s in T. tetragonoides and chromosomal localization of TtAQP s The T. tetragonoides genome was sequenced and submitted to the NCBI database (NCBI accession number: JBBMRK000000000, unreleased data). All of the predicted proteins were annotated with InterProscan ( https://www.ebi.ac.uk/interpro/search/sequence/ ). The conserved MIP domain (PF00230 or IPR000425) was then searched as a model, and the protein sequences containing this domain were screened using HMM3.0 software. The domains were also confirmed using the NCBI CDD program ( https://www.ncbi.nlm.nih.gov/cdd/ ). T. tetragonoides proteins with a MIP domain and their corresponding genes were identified as belonging to the TtAQP family. The obtained TtAQP nucleotide and encoded protein sequences from T. tetragonoides are listed in Table S1 . Gene structure analysis of TtAQP s and homologous analysis of TtAQP family members The gene structures (exon–intron structures) of T. tetragonoides AQP family members were analyzed using the online tool MEME ( http://meme-suite.org/tools/meme ), and the T. tetragonoides genome sequencing data and conserved TtAQP motifs were analyzed based on a predicted value of 10. TBtools [ 53 ] was used to visualize and map the obtained gene structure and the distribution of introns and exons, and the conserved motif information was visualized and mapped. Phylogenetic and conserved motif analysis of the TtAQP proteins The AQP protein sequences of T. tetragonoides were compared with members of bay bean ( C. rosea , CrAQPs) [ 40 ], rice ( Oryza sativa , OsAQPs) [ 54 ], and A. thaliana (AtAQPs) [ 10 ] using the ClustalW algorithm. A phylogenetic tree was constructed with MEGA X software using the neighboring-joining (NJ) method. Based on the results of phylogenetic analysis, TtAQPs were categorized into five classes: PIPs, TIPs, NIPs, SIPs, and XIPs. The nomenclature of these TtAQPs was determined accordingly. The CrAQP, OsAQP, and AtAQP sequences of these three species are listed in Table S1 . Functional amino acid predictions, such as NPA motifs, ar/R filters (H2, H5, LE1, and LE2), and Froger’s positions (P1–P5), were analyzed based on the alignment with AQPs with a known function [ 10 , 40 ]. The 3D structures of TtAQPs were generated with the Phyre 2 server ( https://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=contact ). The subcellular localization patterns of all TtAQPs were predicted with WoLF_PSORT ( https://wolfpsort.hgc.jp/ ) and Plant-mPLoc ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) programs. Duplication event investigation and synteny analysis of TtAQP genes To further analyze the T. tetragonoides AQP family genes and the different duplication patterns of TtAQP members, the duplication events of the AQP genes were analyzed using MCScanX software, and tandem duplications were checked manually according to their gene loci. In addition to the single (SL) gene pattern, the five normal gene duplication patterns, namely whole genome duplication (WGD), tandem duplication (TD, two adjacent duplicate genes), proximal duplication (PD, repeated genes within a 10-gene interval), transposed duplication (repetitive genes composed of ancestors and new loci), and dispersed duplication (DD, repetitive genes that are neither adjacent nor collinear), were determined. Chromosome distribution maps of the covariance of TtAQP s were plotted using Advanced Circos in TBtools software. The number of synonymous substitutions per synonymous site (Ka), the number of non-synonymous substitutions per nonsynonymous site (Ks), and the P-value from Fisher’s exact test of neutrality were calculated using the Nei-Gojobori model with 1000-bootstrap replicates [ 55 ]. A Ka/Ks ratio 1 indicates positive selection. Promoter sequence profiles of TtAQP s The nucleotide sequences 2000 bp upstream from the start codon (ATG) were extracted as the promoter sequence, and CEs’ prediction of the promoter was performed using PlantCARE ( https://bioinformatics.psb.ugent.be/webtools/plantcare/html/ ). The heat-stress elements (HSEs), including 12 putative sequences that have been confirmed in Arabidopsis, rice, maize, and soybean, were also identified manually according to a previous report [ 56 ]. Then, the CE information was further analyzed and shown as a map with TBtools software. The CE sequence information is summarized in Table S2. Expression analysis of TtAQP s Expression data for TtAQP s in T. tetragonoides seedlings treated with heat and other stress treatments, as described previously, were collected from an RNA sequencing (RNA-seq) dataset (unpublished), and the fragments per kilobase of transcript per million mapped reads (FPKM) values were processed according to previous publications [ 52 ]. Briefly, the expression levels [log 2 (FPKM + 1)] of TtAQP s were visualized as heatmaps using TBtools software. The FPKM values for all samples are listed in Table S3. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was also performed to detect the transcript abundance of several TtAQP transcripts according to a previous report [ 52 ]. Briefly, 14 candidate TtAQP genes, namely six TtPIP s ( TtPIP1;1 , TtPIP1;2 , TtPIP2;1 , TtPIP2;2 , TtPIP2;7 , and TtPIP2;8 ), six TtTIP s ( TtTIP1;1 , TtTIP1;2 , TtTIP1;3 , TtTIP1;4 , TtTIP2;1 , and TtTIP2;2 ), and two TtSIP s ( TtSIP1;1 and TtSIP1;2 ), were initially selected based on their RNA-seq data. The expression data for these TtAQP s obtained via qRT-PCR were normalized to the expression of the reference gene TtACT (NCBI accession No.: MH33308). The primers used for qRT-PCR (TtACTRTF/TtACTRTR as the reference gene and other TtAQP -specific primer pairs) are listed in Table S1 . In vivo stress tolerance assay for TtAQP overexpression in yeast The eight candidate TtAQP s ( TtTIP1;1 , TtTIP1;4 , TtTIP2;1 , TtPIP1;1 , TtPIP2;1 , TtPIP2;7 , TtNIP1;2 , and TtSIP1;2 ) were PCR cloned using cDNA from T. tetragonoides as a template. Briefly, the open reading frames of candidate TtAQP s were amplified with gene-specific primer pairs (Table S1 ). The PCR fragments were purified, inserted into the Bam H I and Eco R I sites of yeast expression vector pYES2, yielding recombinant plasmids of TtAQP s-pYES2, and sequenced. Different yeast strains were used in this study, including wild-type (WT) Saccharomyces cerevisiae (BY47471; MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0 ; accession number: Y00000), two H 2 O 2 -sensitive mutant strains skn7Δ (BY4741; MATa; his3Δ1 ; leu2Δ0 ; met15Δ0 ; ura3Δ0 ; YHR206w::kanMX4; accession number: Y02900) and yap1Δ (BY4741; MATa; his3Δ1 ; leu2Δ0 ; met15Δ0 ; ura3Δ0 ; YML007w::kanMX4; accession number: Y00569), which were obtained from Euroscarf ( http://www.euroscarf.de/index.php?name=News ). The standard polyethylene glycol–lithium acetate-based transformation procedure was used for yeast plasmid transformation with amino acid defect screening. The yeast spot assays for NaCl, NaHCO 3 , sorbitol, and oxidative stress tolerance (H 2 O 2 ) were performed as previously described [ 57 ]. For the heat tolerance tests of yeast strains, yeast cultures with different specific OD600 values were placed on a thermostat (50 or 52°C) for different time treatments. The yeast cultures and series of 10-fold diluted yeast liquids were spotted on solid yeast synthetic defect medium plus galactose (SDG) medium plates. The plates were incubated at 30°C for 2–5 days and photographed. Statistical analysis All experiments in this study were repeated independently three times, and the results are shown as the mean ± standard deviation (SD) (n ≥ 3). Pairwise differences between means were analyzed using a Student’s t -test in Microsoft Excel 2021. Results Identification of T. tetragonoides AQP family members In total, 58 TtAQP genes were identified in the T. tetragonoides genome according to our previous genome annotation file (date unpublished) [ 57 ]. These predicted TtAQP proteins all contained the conserved MIP domain. The full-length transcriptome was generated for five T. tetragonoides tissues, namely roots, stem, leaves, flowers, and young seeds, supporting their expression and characterizing them as real genes. Table 1 lists the characteristics of the 58 TtAQPs. All TtAQP members were named according to the classification of AQPs in model plant Arabidopsis. Table 1 Nomenclature and subcellular localization prediction of 58 TtAQPs. Gene name Locus Protein Length (aa) Mw (kD) pI GRAVY TMHs and Topologies No. of Phosphor Sites Prediction for Subcellular Localization WoLF_PSORT Plant-mPLoc TtPIP1;1 Tt03G0018570 285 30.39 9.16 0.453 6/in to in Ser:8 Thr:8 Tyr:4 plas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1 Cell membrane TtPIP1;2 Tt16G0004630 285 30.37 9.17 0.469 6/in to in Ser:8 Thr:9 Tyr:3 plas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1 Cell membrane TtPIP1;3 Tt04G0019610 286 30.57 9.13 0.377 6/in to in Ser:13 Thr:9 Tyr:5 plas: 11, vacu: 3 Cell membrane TtPIP1;4 Tt06G0022590 285 30.44 9.13 0.389 6/in to in Ser:13 Thr:7 Tyr:5 plas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1 Cell membrane TtPIP2;1 Tt01G0010040 287 30.59 8.28 0.464 6/in to in Ser: 14 Thr: 7 Tyr: 2 plas: 12, vacu: 1, E.R.: 1 Cell membrane TtPIP2;2 Tt11G0009920 288 30.70 8.28 0.464 6/in to in Ser:12 Thr:8 Tyr:3 plas: 12, vacu: 1, E.R.: 1 Cell membrane TtPIP2;3 Tt01G0015190 289 30.76 8.29 0.497 6/in to in Ser:13 Thr:6 Tyr:2 plas: 11, E.R.: 2, golg: 1 Cell membrane TtPIP2;4 Tt11G0014700 290 30.80 8.81 0.456 6/in to in Ser:13 Thr:8 Tyr:2 plas: 12, E.R.: 1, golg: 1 Cell membrane TtPIP2;5 Tt07G0003670 287 30.79 8.71 0.461 6/in to in Ser:9 Thr:7 Tyr:5 plas: 14 Cell membrane TtPIP2;6 Tt13G0020820 287 30.79 8.71 0.461 6/in to in Ser:9 Thr:7 Tyr:5 plas: 14 Cell membrane TtPIP2;7 Tt07G0020280 291 32.09 8.84 0.175 6/in to in Ser:15 Thr:10 Tyr:0 plas: 8, vacu: 2, cyto: 1, extr: 1, E.R.: 1, golg: 1 Cell membrane TtPIP2;8 Tt13G0000270 283 30.00 9.18 0.473 6/in to in Ser:10 Thr:6 Tyr:5 plas: 12, vacu: 2 Cell membrane TtPIP2;9 Tt01G0010050 293 31.42 6.95 0.446 6/in to in Ser:14 Thr:10 Tyr:3 plas: 14 Cell membrane TtPIP2;10 Tt11G0009910 255 28.30 6.37 0.240 4/in to in Ser:11 Thr:6 Tyr:3 plas: 13, chlo: 1 Cell membrane TtPIP2;11 Tt07G0003660 283 29.99 9.28 0.482 6/in to in Ser:10 Thr:6 Tyr:5 plas: 11, vacu: 3 Cell membrane TtTIP1;1 Tt01G0009310 255 26.28 5.81 0.745 6/in to in Ser:7 Thr:5 Tyr:3 plas: 11, vacu: 2, E.R.: 1 Vacuole TtTIP1;2 Tt11G0010640 255 26.27 5.81 0.731 6/in to in Ser:7 Thr:4 Tyr:3 plas: 11, vacu: 2, E.R.: 1 Vacuole TtTIP1;3 Tt03G0000820 251 25.76 5.62 0.861 6/in to in Ser:8 Thr:2 Tyr:2 vacu: 13, plas: 1 Vacuole TtTIP1;4 Tt04G0000850 252 25.88 5.89 0.836 6/in to in Ser:10 Thr:3 Tyr:3 vacu: 14 Vacuole TtTIP1;5 Tt09G0011690 213 22.03 5.99 0.844 5/out to in Ser:4 Thr:3 Tyr:3 plas: 6, vacu: 5, extr: 1, E.R.: 1, golg: 1 Vacuole TtTIP1;6 Tt15G0007390 252 26.19 6.11 0.784 6/in to in Ser:4 Thr:3 Tyr:3 plas: 8, vacu: 6 Vacuole TtTIP2;1 Tt07G0018250 248 25.22 6.11 0.875 6/in to in Ser:13 Thr:4 Tyr:3 vacu: 8, plas: 6 Vacuole TtTIP2;2 Tt13G0002320 248 25.22 6.00 0.823 6/in to in Ser:13 Thr:5 Tyr:3 vacu: 9, plas: 5 Vacuole TtTIP2;3 Tt04G0023790 250 25.21 5.42 0.969 6/in to in Ser:12 Thr:3 Tyr:1 vacu: 12, plas: 1, extr: 1 Vacuole TtTIP2;4 Tt06G0013740 248 24.94 5.09 1.003 6/in to in Ser:13 Thr:3 Tyr:0 vacu: 13, plas: 1 Vacuole TtTIP2;5 Tt04G0023800 250 25.25 4.86 0.931 6/in to in Ser:13 Thr:2 Tyr:3 vacu: 14 Vacuole TtTIP2;6 Tt06G0013750 250 25.17 5.39 0.965 6/in to in Ser:13 Thr:1 Tyr:4 vacu: 12, plas: 1, extr: 1 Vacuole TtTIP3;1 Tt05G0011610 257 27.05 7.07 0.629 6/in to in Ser:4 Thr:6 Tyr:3 plas: 8, vacu: 4, chlo: 1, E.R.: 1 Vacuole TtTIP3;2 Tt12G0014560 257 27.05 7.07 0.629 6/in to in Ser:4 Thr:6 Tyr:3 plas: 8, vacu: 4, chlo: 1, E.R.: 1 Vacuole TtTIP4;1 Tt09G0007910 231 24.19 6.63 0.819 6/in to in Ser:8 Thr:3 Tyr:2 vacu: 11, plas: 2, extr: 1 Vacuole TtTIP4;2 Tt15G0011210 251 26.19 7.03 0.785 6/in to in Ser:8 Thr:5 Tyr:1 plas: 7, vacu: 6, E.R.: 1 Vacuole TtTIP5;1 Tt06G0009470 255 26.30 8.45 0.535 6/in to in Ser:15 Thr:8 Tyr:3 E.R.: 5, plas: 3.5, cyto_plas: 2.5, chlo: 2, mito: 1, vacu: 1, pero: 1 Cell membrane/Vacuole TtTIP5;2 Tt13G0013390 206 21.52 6.14 0.578 2/in to in Ser:8 Thr:5 Tyr:1 E.R.: 3, pero: 3, chlo: 2, cyto: 2, plas: 2, vacu: 1, golg: 1 Cell membrane TtNIP1;1 Tt08G0012500 294 31.21 9.08 0.380 6/in to in Ser:16 Thr:9 Tyr:0 plas: 11, vacu: 1, E.R.: 1, golg: 1 Cell membrane TtNIP1;2 Tt10G0005880 294 31.18 9.36 0.377 6/in to in Ser:17 Thr:9 Tyr:0 plas: 11, vacu: 2, golg: 1 Cell membrane TtNIP2;1 Tt03G0021270 285 31.01 8.18 0.571 6/in to in Ser:13 Thr:3 Tyr:1 plas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1 Cell membrane TtNIP2;2 Tt03G0021280 285 31.01 8.18 0.571 6/in to in Ser:13 Thr:3 Tyr:1 plas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1 Cell membrane TtNIP2;3 Tt03G0021260 285 31.01 8.18 0.571 6/in to in Ser:13 Thr:3 Tyr:1 plas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1 Cell membrane TtNIP3;1 Tt11G0017300 288 30.52 9.19 0.459 6/in to in Ser:18 Thr:8 Tyr:1 plas: 11, vacu: 2, E.R.: 1 Cell membrane TtNIP4;1 Tt03G0021290 157 16.43 9.73 0.687 4/in to in Ser:9 Thr:6 Tyr:0 vacu: 11, plas: 1, extr: 1, golg: 1 Cell membrane TtNIP4;2 Tt16G0007250 285 30.34 7.66 0.469 6/in to in Ser:16 Thr:11 Tyr:3 plas: 9, vacu: 2, extr: 1, E.R.: 1, golg: 1 Cell membrane TtNIP5;1 Tt03G0015410 311 32.38 7.13 0.483 6/in to in Ser:16 Thr:10 Tyr:1 plas: 7, vacu: 3, E.R.: 3, golg: 1 Cell membrane TtNIP5;2 Tt16G0010870 308 32.08 7.13 0.460 6/in to in Ser:15 Thr:10 Tyr:1 plas: 8, E.R.: 3, vacu: 2, golg: 1 Cell membrane TtNIP6;1 Tt07G0016040 307 31.69 6.07 0.516 6/in to in Ser:9 Thr:16 Tyr:1 plas: 11, cyto: 1, extr: 1, vacu: 1 Cell membrane TtNIP6;2 Tt13G0004320 304 31.33 6.07 0.532 6/in to in Ser:10 Thr:15 Tyr:1 plas: 11, vacu: 2, extr: 1 Cell membrane TtNIP7;1 Tt04G0020740 253 26.22 6.90 0.712 6/in to in Ser:11 Thr:9 Tyr:1 vacu: 7, plas: 4, golg: 3 Cell membrane TtNIP7;2 Tt04G0020840 253 26.22 6.90 0.712 6/in to in Ser:11 Thr:9 Tyr:1 vacu: 7, plas: 4, golg: 3 Cell membrane TtNIP7;3 Tt06G0021490 253 26.30 6.05 0.720 6/in to in Ser:8 Thr:10 Tyr:1 vacu: 7, plas: 4, golg: 3 Cell membrane TtNIP8;1 Tt06G0018640 325 34.76 8.45 0.413 6/in to in Ser:17 Thr:4 Tyr:2 plas: 6, golg: 4, vacu: 2, E.R.: 2 Cell membrane TtNIP8;2 Tt06G0018660 325 34.83 8.78 0.388 6/in to in Ser:17 Thr:4 Tyr:2 plas: 8, golg: 3, E.R.: 2, vacu: 1 Cell membrane TtNIP8;3 Tt10G0007570 229 23.88 6.89 0.835 6/in to in Ser:10 Thr:3 Tyr:2 vacu: 14 Cell membrane/Vacuole TtNIP8;4 Tt13G0018950 229 23.89 6.95 0.855 6/in to in Ser:8 Thr:3 Tyr:2 vacu: 14 Cell membrane/Vacuole TtSIP1;1 Tt09G0010400 248 26.50 9.63 0.684 6/in to in Ser:9 Thr:11 Tyr:2 plas: 9, vacu: 4, cyto: 1 Cell membrane TtSIP1;2 Tt15G0008730 248 26.49 9.57 0.680 6/in to in Ser:9 Thr:11 Tyr:2 plas: 8, vacu: 5, cyto: 1 Cell membrane TtSIP2;1 Tt02G0020900 233 25.49 9.48 0.652 6/in to in Ser:7 Thr:5 Tyr:0 vacu: 6, plas: 5, E.R.: 3 Cell membrane TtSIP2;2 Tt14G0020550 233 25.41 9.41 0.682 6/in to in Ser:10 Thr:5 Tyr:0 vacu: 6, plas: 5, E.R.: 2, extr: 1 Cell membrane TtXIP1;1 Tt07G0019520 246 26.13 8.52 0.975 6/in to in Ser:9 Thr:9 Tyr:1 vacu: 13, extr: 1 Cell membrane/Vacuole TtXIP1;2 Tt13G0001000 321 34.48 7.62 0.764 6/in to in Ser:14 Thr:10 Tyr:1 plas: 6, vacu: 6, E.R.: 2 Cell membrane There were 15 TtPIPs, 18 TtTIPs, 19 TtNIPs, 4 TtSIPs, and 2 TtXIPs, and the TtPIPs were further divided into 4 TtPIP1s and 11 TtPIP2s (Table 1 ). Based on the analysis of the physical and chemical properties of the TtAQPs, the protein lengths of the TtAQPs ranged from 157 (TtNIP4;1) to 325 aa (TtNIP8;1 and TtNIP8;2), with molecular weights ranging from 16.43 to 34.83 kDa. The isoelectric point values of TtAQPs were between 4.86 (TtTIP2;5) and 9.57 (TtSIP1;2), and most of them (35 of 58) were above 7, indicating that they are basic proteins. Additionally, the grand average of hydropathicity (GRAVY) was between 0.175 (TtPIP2;7) and 1.003 (TtTIP2;4), indicating that they are all hydrophobic proteins (GRAVY > 0). The phosphorylation modification for AQPs is one of the key factors determining the subcellular distribution and trafficking of proteins, which further determines the biochemical functions of these membrane proteins [ 58 ]. All TtAQPs had more than one phosphorylation amino acid site, including serine, threonine, and tyrosine. The subcellular localization prediction for TtAQPs was performed with different programs, including WoLF_PSORT and Plant-mPLoc (Table 1 ). As plasma membrane and endomembrane intrinsic proteins, the subcellular localization prediction for TtAQPs was roughly symmetrical with their nomenclature, especially the prediction values from the WoLF_PSORT program. The presence of transmembrane domains in each TtAQP member provides the necessary conditions for water molecular cross-membrane transportation (Figure S1 ), which depends on the pore formed by a series of membrane helices linked by helix loops. The subcellular localization of specific TtAQPs might be determined more specifically by post-translational modifications or protein interactions [ 59 ]. The multiplicity of TtAQPs’ localization and modifications further determines the diversity and complexity of their biochemical and biological functions. The features of TtAQPs were also predicted with the InterPro online program. As shown in Table 1 and Figure S1 , all TtAQPs were membrane-anchored proteins with 2–6 trans-membrane α-helices, which form pores for water or other small molecules transported as heterotetramers [ 15 ]. The MIP conserved domain was present in most TtAOP proteins (Figure S2). The N-terminal of the TtAOPs was usually a signal peptide, which might directly determine the subcellular localization of each TtAQP in vivo . Evolutionary characterization of AQP proteins To uncover their classification and evolutionary relationships, an unrooted phylogenetic tree from the deduced protein sequences of all TtAQPs was constructed (Fig. 1 ). Almost all TtAQP members were in pairs, except TtPIP2;11, TtNIP2;3, TtNIP3;1, and TtNIP7;3. The sequence similarities between two members in the same pair were extremely high, indicating that the genetic homology of these genes is very high. XIPs are uncharacterized AOPs [ 16 ]. Here, the two TtXIPs were very similar to TtPIPs, further suggesting their possible similar evolutionary origins. To study the evolutionary relationship of AQP family proteins among plant species, an ML tree was created with AQP proteins from A . thaliana (dicotyledonous model plant), O . sativa (monocotyledonous model plant), and C . rosea (typical halophyte). Five distinct clades or subfamilies (PIP, TIP, NIP, SIP, and XIP) were clearly distinguished (Fig. 1 ), and PIPs, TIPs, and NIPs were relatively bigger, while the XIP clade was smallest. Due to T. tetragonoides being a dicotyledonous plant, TtAQPs were more similar to AtAQPs and CrAQPs, and the total number of TtAQPs (58) was much greater than that of AtAQPs (35), OsAQPs (34), and CrAQPs (37). Their genome sizes did not show large differences ( T. tetragonoides : 400 Mb; Arabidopsis: 125 Mb; Rice: 385 Mb; C . rosea : 535 Mb). We also compared the number of AQP genes in T. tetragonoides with that in other plants (Table 2 ), including two Brassicaceae species, nine Leguminosae species, five Gramineae species, and four other typical special habitat plants. The number of AQP genes in all of these typical diploid species was below 50, and only the paleotetraploid genome of soybean ( G . max ) contained more AQP members (72). The number of TtAQP s was relatively high, although this species is a typical diploid with a normal genome size (400 Mb). Table 2 The numbers of AQP genes in different plant species. Family/Category Species Total No. The No. of AQP s in sub-families PIPs TIPs NIPs SIPs XIPs Brassicaceae Arabidopsis thaliana 35 13 10 9 3 0 Eutrema salsugineum 35 12 11 9 3 0 Leguminosae Canavalia rosea 37 11 10 11 4 1 Cicer arietinum 40 9 12 16 3 0 Glycine max (paleotetraploid) 72 24 24 17 8 2 Medicago truncatula 45 10 14 17 4 0 Gramineae Oryza sativa 34 12 10 10 2 0 Setaria italica 39 12 11 13 3 0 Special habitat plants Kandelia obovata 34 13 10 6 4 1 Avicennia marina 46 18 15 7 4 2 Ziziphus jujuba 36 10 10 12 3 1 Populus euphratica 22 6 9 4 3 0 Tetragonia tetragonoides 58 15 18 19 4 2 Table 3 Conserved amino acid residues (Asn-Pro-Ala, NPA) motifs, aromatic/arginine (ar/R) filters and Froger’s positions (FPs) and trans-membrane (TM) domains of TtAQPs in T. tetragonoides . Protein No. of aa TM NPA (LB) NPA (LE) NPA space Ar/Rfilters Froger’s residues H2 H5 LE1 LE2 P1 P2 P3 P4 P5 TtPIP1;1 285 6 NPA NPA 118 F H T R Q S A F W TtPIP1;2 285 6 NPA NPA 118 F H T R Q S A F W TtPIP1;3 286 6 NPA NPA 118 F H T R Q S A F W TtPIP1;4 285 6 NPA NPA 118 F H T R Q S A F W TtPIP2;1 287 6 NPA NPA 118 F H T R Q S A F W TtPIP2;2 288 6 NPA NPA 118 F H T R MQ S A F W TtPIP2;3 289 6 NPA NPA 118 F H T R Q S A F W TtPIP2;4 290 6 NPA NPA 118 F H T R Q S A F W TtPIP2;5 287 6 NPA NPA 118 AF VH AT R YQ S A Y L TtPIP2;6 287 6 NPA NPA 118 AF VH AT R YQ S A Y L TtPIP2;7 291 6 NPA NPA 118 F H T R QM S A F W TtPIP2;8 283 6 NPA NPA 118 F H T R M S A F W TtPIP2;9 293 6 NPA NPA 118 LF H T R Q S A F W TtPIP2;10 255 4 NPA NPA 118 F H T R Q S A F W TtPIP2;11 283 6 FVA NPA 118 F H T R Q S A F W TtTIP1;1 255 6 NPA NPA 111 H I A V T S A Y W TtTIP1;2 255 6 NPA NPA 111 H I A V T S A Y W TtTIP1;3 251 6 NPA NPA 111 H I A RV T AS A Y W TtTIP1;4 252 6 NPA NPA 111 H I A RV T AS A Y W TtTIP1;5 213 5 NPA NPA 110 H I A V T S A Y W TtTIP1;6 252 6 NPA NPA 111 H I A V T S A Y W TtTIP2;1 248 6 NPA NPA 111 H I AG R S S A Y W TtTIP2;2 248 6 NPA NPA 111 H I AG R S S A Y W TtTIP2;3 250 6 NPA NPA 111 H I AG R T S A Y W TtTIP2;4 248 6 NPA NPA 111 H I AG R T S A Y W TtTIP2;5 250 6 NPA NPA 111 H I AG R T S A Y W TtTIP2;6 250 6 NPA NPA 111 H I AG R T S A Y W TtTIP3;1 257 6 NPA NPA 111 H I A VR T SA A Y W TtTIP3;2 257 6 NPA NPA 111 H I A VR T SA A Y W TtTIP4;1 231 6 NPA NPA 95 H I IA R T S A Y W TtTIP4;2 251 6 NPA NPA 111 H I IA R T S A Y W TtTIP5;1 255 6 NPA NPA 110 N V AG Y T S A Y W TtTIP5;2 206 2 NPV NPA 110 VM V FG RY VT CL A FY W TtNIP1;1 294 6 NPA NPA 110 SW IV A R YF TS A Y MI TtNIP1;2 294 6 NPS NPV 109 AW IV A R F TS A Y LI TtNIP2;1 285 6 NPA NPV 109 SW IA A R F TS A Y CV TtNIP2;2 285 6 NPA NPV 109 SW IA A R F TS A Y CV TtNIP2;3 285 6 NPA NPV 109 AW IA A R F TS A Y LV TtNIP3;1 288 6 NPA NPA 108 LG NS AG SR FL VT A Y WM TtNIP4;1 157 4 NPA \ \ G\ SV A R LF TS A Y MI TtNIP4;2 285 6 NPA NPA 109 SW IV A R YF TS A Y MI TtNIP5;1 311 6 NPS NPV 108 \A VI A YR TF LT A Y WL TtNIP5;2 308 6 NPS NPV 108 WA AI A R F ST A Y VL TtNIP6;1 307 6 NPA NPV 108 S I AG R YF T A Y MC TtNIP6;2 304 6 NPA NPV 108 FS HI TG R QF ST A FY WC TtNIP7;1 253 6 NPA NPA 108 LS NI A AR FY VT A Y WM TtNIP7;2 253 6 NPA NPA 108 VS VI IA NR MY AT A Y WM TtNIP7;3 253 6 NPA NPA 108 VS VI IA NR MY AT A Y WM TtNIP8;1 325 6 NPA NPA 110 WA V AG R FY S A Y IL TtNIP8;2 325 6 NPA NPA 110 WA V AG R FY S A Y IL TtNIP8;3 229 6 NPA NPA 110 WA AV AG R FY S A Y VL TtNIP8;4 229 6 NPA NPA 110 WA AV AG R FY S A Y VL TtSIP1;1 248 6 NPT NPA 112 \V V AP RN FM SA A Y IW TtSIP1;2 248 6 NPT NPA 112 WV V AP RN FM SA A Y IW TtSIP2;1 233 6 NPL NPA 108 AS VN AG RS YL SV A Y LW TtSIP2;2 233 6 NPL NPA 108 AS VN AG RA YL SV A Y LW TtXIP1;1 246 6 NPV NPA 134 V VI FA R VN C A F W TtXIP1;2 321 6 NPV NPA 134 FV HI TA R QN SC A F W Chromosomal locations and TtAQP gene structures The chromosome maps localizing TtAQP s were constructed with the purpose of investigating the gene family evolutionary relationship. There are 16 pairs of chromosomes in the T. tetragonoides genome. TtAQP genes were found on all chromosomes, but their distribution was uneven. Chromosomes 02, 05, 08, 12, and 14 each had only one TtAQP gene. Chromosome 10 contained two TtAQP s, and chromosomes 09, 15, and 16 each had three TtAQP s. Chromosome 01 had four, and chromosomes 03, 04, and 11 had five. Chromosome 07 had six, and chromosomes 06 and 13 had the most, with seven (Fig. 2 ). The exon–intron structures of genes are essential for elucidating the possible molecular evolution of plant gene family members. The divergence of gene structures mainly occurs in duplicate gene evolution and gene sibling paralogs, which can result in functional gain or loss through specific exonization/pseudoexonization and insertion/deletion [ 60 ]. Based on phylogenetic and gene structure analyses (Fig. 3 ), most conjugated TtAQP s presented similar exon–intron patterns, despite the fact that the lengths of introns and untranslated regions (UTRs) were slightly different. Only TtTIP1;5 / TtTIP1;6 , TtTIP5;1 / TtTIP5;2 , TtXIP1;1 / TtXIP1;2 , TtPIP2;9 / TtPIP2;10 , and TtNIP4;1 / TtNIP4;2 showed different gene structures. This high degree of similarity in protein sequences and gene structures further indicates that homologous gene duplication resulted in TtAQP gene family amplification. Duplication event investigation Gene duplication events for TtAQPs were investigated (Fig. 4 , Table S2). As a main driving force of genome evolution, the phenomenon of gene duplication is widespread among the genomes of all organisms. To analyze the evolutionary process among TtAQP family genes, genome-wide duplication events among these members were identified and analyzed, and MCScan X software was used to draw Circos plots. This analysis showed that 46 genes formed genome-wide duplication pairs, that the 23 pairs of homologous genes were segmentally duplicated, and that the duplication pairs resulted in many homologues of TtAQPs among chromosomes, increasing the likelihood of evolution (Fig. 4 ). These findings suggest that gene duplication events play an important role in TtAQP expansion. We also found that most TtAQPs were generated by WGD (47 of 58 TtAQPs ), and only TtPIP2;5, TtTIP2;5, TtTIP2;6, and TtNIP2;1 were generated by TD, and the PD pattern was only observed for TtNIP7;2 and TtNIP8;2. TtPIP2;3, TtPIP2;4, TtTIP3;1, TtTIP3;2, and TtNIP3;1 showed the DD pattern. Throughout evolution, evolutionary forces and natural pressures inevitably affect the duplicated genes, showing different patterns. To understand the evolutionary divergence between paralogous gene pairs, Ka/Ks analysis was carried out in the TtAQP gene family. A Ka/Ks ratio of more than 1 (Ka/Ks > 1) suggests positive selection pressure (non-purifying), and a ratio less than 1 (Ka/Ks < 1) indicates negative (purifying) selection pressure. A ratio equal to 1 (Ka/Ks = 1) indicates neutral selection. All paralogous genes showed a Ka/Ks ratio much less than 1 (Ka/Ks < 1), suggesting negative or purifying selection on duplicated TtAQP genes (Table S3). Cis -acting elements (CEs) of the TtAQP promoter sequences The gene expression patterns are closely related to the biological functions, and transcriptional regulation is directly under the control of the gene promoters. Prediction of the primary structures of TtAQP gene promoter regions (ATP upstream 2000 bp) can clarify the transcriptional regulatory functions of gene family members. The CEs located in the promoter regions of the 58 TtAQP members were analyzed (Fig. 5 ). PlantCARE predicted that all promoter regions of TtAQPs presented various functions for CEs, including biotic and abiotic stress responses. The MYB- (including MBS) and MYC-recognition sites were found in almost all TtAQP gene promoter regions, indicating the basic regulatory roles mediated by the transcription factors (TFs), MYBs and MYCs. Plant MYBs and MYCs are ubiquitous TFs, and many previous reports have shown that these TFs are involved in plant salt and drought responses [ 61 ]. Additionally, HSEs are typically recognized by heat shock TFs (HSFs), thereby regulating the expression of the target genes. In all TtAQP promoter regions, there were many HSEs, indicating that the water imbalance caused by unpredictable heat treatment could be alleviated by the multiple water transport activities of TtAQPs, and this occurs because of the heat regulated expression patterns of TtAQP s mediated by the HSEs in their promoter regions. In addition, the hormone-response CEs, including ABREs (abscisic acid-responsive), TGACG- and CGTCA- motifs (jasmonic acid-responsive), TCA-element (salicylic acid-responsive), TATC-box (gibberellic acid-responsive), and AuxRR-core (auxin-responsive), also irregularly existed in the promoter regions of TtAQP s (Fig. 5 ; Table S4), indicating that TtAQP expression was influenced by hormones and related development or stress responses. The diversity of multiple CEs in TtAQP promoter regions further explained the possible roles for water transport in vivo mediated by specific TtAQPs, which was affected by their specific expression patterns under the control of their own promoters. The highly precise regulatory mechanism for TtAQP expression further reflects the complexity of functions of these water channel proteins, such as in mediating plant growth, development, and biotic/abiotic stress responses. Global expression profiles of TtAQP s in different tissues and plants under stress To reveal the global expression profiles of TtAQP genes, RNA-seq data for five tissues/developmental stages with three biological repeats each were examined (Fig. 6 ; Table S5). Plant AQPs have been shown to be engaged in plant growth and development, although the fundamental roles of AQPs involve the transport of H 2 O, other small molecules, and some specific chemical elements [ 62 ]. In some plants, their AQP subfamily members show similar expression patterns, indicating their synergistic roles in the transport of water or other molecules in some organs. For instance, the mRNA of PIPs and TIPs is abundant in all organs in many plant species [ 43 ]. Our result showed that the expression of TtAQPs among different tissues, including roots, stems, leaves, flowers, and young seeds, was organ specific, and the TtPIPs and TtTIPs presented relatively higher expression levels in all organs (Fig. 6 ; Figure S3). Some TtPIP and TtTIP members showed remark- ably high expression levels in the stems and leaves of T. tetragonoides plants, and the TtTIP3;1 / TtTIP3;2 gene pair showed a much higher expression level in flowers than in other organs. These specific expression patterns further demonstrate the important roles of TtAQPs in the growth and development of T. tetragonoides . The disturbed water balance of plants in vivo is often caused by a series of abiotic stressors, which are the main limiting factors for plants’ adaptation to environmental conditions, such as osmotic stress generated from high salinity/alkalinity, drought, and heat. To determine the possible functions of TtAQPs in abiotic stress response, we examined the effects of heat, salt, alkaline, and high osmotic (mimicking drought) treatments on the expression of all TtAQP genes (Fig. 7 ). In underground root tissues, heat stress decreased the expression of most TtAQPs , while in aboveground parts (stems and leaves), heat stress increased the expression levels of some TtAQP members, including TtTIP1;1 / 1;2 , TtTIP1;3 / 1;4 , TtTIP2;1 / 2;2 , TtPIP1;1 / 1;2 , TtPIP1;3 / 1;4 , TtPIP2;1 / 2;2 , and TtTIP2;7 / 2;8 gene pairs. Additionally, in root tissues, the salt, alkalinity, and high osmotic stress decreased the expression of most TtAQPs , while in stems and leaves, some TtAQPs , such as TtTIP1;1 , TtTIP2;1, TtTIP2;1 / 2;2 , TtPIP2;3 , TtPIP2;8 , and TtPIP2;9 , were induced by these stress treatments, especially the short-term treatments (2 h). Unexpectedly, TtSIP1;1 showed upregulated expression in stems and leaves under long-term treatments (48 h), indicating that TtSIP1;1 is involved in these stress response pathways. We further investigated the expression patterns of TtAQPs under salt, alkalinity, drought (high osmotic), and heat stress by qRT-PCR with more detailed stress treatment times (Fig. 8 ). Most of the detected TtAQP s were upregulated under salt, alkalinity, and high osmotic stress at different time points or in different tissues, while only TtPIP2;2 , TtTIP2;2 , and TtSIP1;2 showed downregulated expression patterns under all challenges, further demonstrating the relationship between the mRNA abundance of TtAQP s and water transport dynamic changes in plants affected by stress treatment in vivo . Abiotic stress tolerance of yeast heterologously expressing TtAQP s The yeast heterogeneous expression system has become an important approach for characterizing AQP transmembrane transportation [ 63 ]. Here, we selected eight TtAQP members and determined their possible roles in abiotic stress tolerance when overexpressed in different yeast strains. When expressed in WT yeast cells, TtTIP1;2 , TtTIP1;4 , TtTIP2;1 ; TtPIP1;1 , TtPIP2;1 , TtPIP2;7 , TtNIP1;2 , and TtSIP1;2 enhanced the salt tolerance of WT under high NaCl stress (1.25 and 1.5 M NaCl). A higher salinity stress intensity (1.75 M NaCl) inhibited the growth of yeast expressing the pYES2 empty vector (EV) or eight TtAQPs (Fig. 9 A). Under alkali stress, NaHCO 3 treatment did not cause a distinguished growth status for yeast expressing EV or eight TtAQPs (Figure S4A). The high osmotic stress caused by different sorbitol concentrations also affected the growth of WT expressing TtAQPs to a certain extent, and TtNIP1;2 increased the sensitivity of WT to sorbitol. TtPIP1;1 and TtSIP1;2 enhanced the tolerance of WT to high osmotic stress compared to EV (Fig. 9 B). For heat stress tolerance, TtPIP1;1 and TtSIP1;2 also improved the survival rate of WT yeast after 50℃ heat shock (20 min), while TtTIP1;4 , TtTIP2;1 , TtPIP2;1 , TtPIP2;7 , and TtNIP1;2 enhanced the sensitivity of WT yeast to heat shock (Fig. 9 C). The H 2 O 2 -sensitive yeast mutants skn7Δ and yap1Δ were also used to detect the transportation function of these eight TtAQP members (Figs. 10 A and S4B). Both yeast skn7 and yap1 encode TFs involved in oxidative stress responses [ 64 ]. Our previous study confirmed that both skn7Δ and yap1Δ were sensitive to heat stress [ 65 ]. In this study, the selected eight TtAQP members caused differing H 2 O 2 sensitivities in both skn7Δ (Fig. 10 A) and yap1Δ (Figure S4B), indicating that these TtAQPs transport H 2 O 2 but in different cellular membranes in yeast. Additionally, except TtSIP1;2 , the other seven TtAQPs enhanced the sensitivity of skn7Δ yeast to heat shock (Fig. 10 B). These results were similar to those of the heat sensitivity test in WT yeast ( TtPIP1;1 caused similar tolerance as TtSIP1;2 ), which may be ascribed to the skn7 mutation and related downstream signal pathways differing from those in WT. Discussion Tetragonia tetragonoides is an atypical, succulent endo-reoretohalophyte with excellent salinity, alkalinity, drought, and heat tolerance. As a rare halophytic tropical leafy vegetable, it has emerged as a novel health food due to its salt bladders, which contain essential mineral elements and some secondary metabolites [ 4 ]. T. tetragonoides grows wild on many tropical beaches, coastal regions, and islands, which present extreme adversities for normal plant growth, such as high salinity/alkalinity, seasonal drought, and high temperatures caused by direct sun-exposure. T. tetragonoides has adapted to its low altitude and extreme environments through morphological, physiological, and molecular mechanisms. The typical features of T. tetragonoides in salt bladders over the whole plant surface, including the leaf epidermis and surfaces of petioles and stems, are among the most distinctive salt-resistance features in higher plants, and about half of halophytes have characteristic salt bladders on their surfaces [ 66 ]. Salt bladders can increase the salt tolerance of plants by sequestering excessive Na + away from metabolically active cells and storing water for osmotic adjustment, thereby providing plants with an opportunity to survive in saline environments [ 66 ]. The epidermal cells containing salt bladders usually expand greatly in volume, even up to 1000 times larger than epidermal bladder cells (EBCs). The large vesicles/vacuoles in EBCs usually contain large amounts of salt ions or secondary metabolites, which can be transported quickly with water by AQPs to maintain appropriate osmotic pressure in vivo . For example, Atriplex canescens is a typical C4 secretohalophyte with salt bladders on the leaves. AcAQPs in leaves showed increased expression patterns after NaCl stress, facilitating water balance in seedlings under low water potential conditions [ 67 ]. Mesembryanthemum crystallinum (also called ice plant, Aizoaceae) is a closely related species of T. tetragonoides , and McAQPs are precisely regulated during salt stress to maintain water balance [ 68 ]. Since T. tetragonoides can grow in harsh habitats and have strong water storage capacity to endure the high salinity/alkalinity and drought stress, we suspect that TtAQPs play roles in the adaptation to such harsh environments, and identification of the TtAQP family is necessary to elucidate the possible molecular adaptation mechanisms of this special habitat species. Plant AQPs belong to the MIP family and mainly function as channel proteins known for their abilities to conduct water and/or small molecules across biological membranes [ 15 , 16 ]. In recent years, studies have focused on the possible regulatory roles of plant membrane transporters and biotic or abiotic stress responses [ 69 ]. Considering the growth and ecological adaptability of plants, the water deficit caused by external environmental factors significantly affects the survival and development of plant species. The damage caused by water deficit can be mitigated through the channels’ formation of various transporter proteins, such as AQPs, evolving specific physiological mechanisms adapted to adversity stress. In this study, we performed a genome-wide identification of the TtAQP family using bioinformatics methods and analyzed the phylogenetic relationships among TtAQPs and AQP family members of other plant species. Additionally, the gene structure and chromosome distribution of TtAQPs were determined through covariance analysis, and their possible duplication patterns were determined. In this study, 58 TtAQP family members were identified from the T. tetragonoides genome. They included 15 TtPIPs , 18 TtTIPs , 19 TtNIPs , 4 TtSIPs , and 2 TtXIPs . Overall, the number of TtAQP gene family members in the diploid T. tetragonoides genome was greater than that found in many other typical diploid plant species (Table 2 ), which might be due to the duplication patterns of different genes for adaptive evolution and further functional differentiation of TtAQP family in this species. The abundance of TtAQP isoforms in T. tetragonoides is also attributed to the need for organelle- or tissue-development specificity, as well as the requirement for the precise control of water transport under specific environmental conditions. Similarly, although some plant AQPs have been named according to their possible subcellular localization, such as PIP for plasma membrane intrinsic protein, TIP for tonoplast intrinsic protein, and SIP for possible endoplasmic reticulum membrane localization, their virtual cellular localization might be dynamic and adjustable. Plant AQPs belong to membrane-anchored proteins with 2–6 trans-membrane α-helices, which form pores for transporting water or other small molecules, such as heterotetramers [ 15 ], and determine their subcellular dispersal. As shown in Table 1 , the phosphorylation modification sites for each TtAQP member were specific, and this post-translational level modification further determines the functional specificity for each TtAQP. The functional management of plant PIPs depends on four levels: (I) transcriptional level: the regulatable expression by some specific factors; (II) subcellular trafficking level: including exocytosis, endocytosis, autophagic degradation, and proteasomal degradation; (III) post-translational level: mainly referring to protein modifications; and (IV) gating regulation: including Ca 2+ ion levels, cytosolic pH, and reactive oxygen species (ROS) [ 70 ]. Tables 1 and 2 show that the T. tetragonoides genome has more AQP family members (58) than most other diploid species (except the paleotetraploid soybean). Further phylogenetic analysis (Figs. 1 and 3 ) and collinearity analysis (Fig. 4 ) also demonstrated that most TtAQP members were present in pairs. The increasing number of TtAQP s might be closely related to the morphological development and ecological adaptability of T. tetragonoides . Considering that the regulation of gene expression is closely related to the biological functions of the genes, we examined the features of promoters and the expression patterns of TtAQP s. Previous research has revealed that abiotic stress, such as salt, drought, low temperature, dry air, ion, and temperature stress, in the surrounding environment causes water absorption difficulties in plants, thereby disturbing the water balance or even causing water deficit in vivo and promoting AQP expression [ 71 ]. Without exception, the predicted promoter regions of TtAQPs contained multiple CEs related to abiotic stress responses (Fig. 5 ), including MYB- (including MBS) and MYC-recognition sites, HSEs, ABREs, and other hormone- and development-related CEs. The multiformity of CEs in TtAQP s’ promoter regions confers the complexity and regulation of TtAQPs ’ expression, making T. tetragonoides plants more sensitive to ambient water conditions. In the present study, the RNA-seq assays were also performed with different T. tetragonoides organs considering both their developmental functions (Fig. 6 ) and abiotic stress responses (Fig. 7 ). Based on these results, we conclude that some specific TtAQP members were closely related to special development stages or organs of T. tetragonoides , such as TtPIP2;5 / TtPIP2;6 in the roots and TtTIP3;1 / TtTIP3;2 in fruit (Fig. 6 ). Additionally, the expression changes in T. tetragonoides roots, stems, and leaves under different stress treatments also occurred in TtPIP and TtTIP subgroups (Figs. 7 and 8 ), suggesting that the TtAQP members of these two subfamilies might play important roles in stress responses. To date, the AQP gene family has been characterized in an increasing number of species; thus, we paid particular attention to this gene family in special habitat plant species [ 43 – 51 ]. Although the stress factors that caused the water imbalance can influence AQP expression to varying degrees, with up- or down-regulation in different organs, further functional confirmation with transgenic overexpression in other plant species often presents different effects. Deeper functional research on plant AQPs can be performed by reduced expression via antisense or RNA interference-mediated changes in the same plant species or by mutant AQPs . Related transgenic research on plant AQPs ’ role involved in salt and drought tolerance has emerged greatly in recent years. In particular, the AQPs isolated from special habitat plants held our attention. For example, SpAQP1 (PIP2 group) from the halophyte Sesuvium portulacastrum increased salt tolerance in transgenic tobacco, probably by improving the efficiency of water transportation, thereby enhancing the antioxidative activity of transgenic plants [ 72 ]. Stipa purpurea better adapts to the stressors in the natural environment, such as drought and cold stress, and transgenic Arabidopsis overexpressing SpPIP1 (PIP1 group) presented enhanced drought (high osmotic) tolerance compared to WT control plants, indicating that this gene is a candidate gene for crop genetic improvement concerning drought tolerance [ 73 ]. Jojoba ( Simmondsia chinensi ) is a typical desert plant with good tolerance to drought, salinity, and nutritional deficiency; when ScPIP1 (PIP1 group) was overexpressed in Arabidopsis, the transgenic plants exhibited higher drought and salt tolerance than WT control plants [ 74 ]. Overexpression of TsPIP1;1 (PIP1 group) from Thellungiella salsuginea (also named E. salsugineum ) in rice increased its salt tolerance [ 75 ]. The expression of SbPIP2 (PIP2 group) in halophyte Salicornia brachiata was rapidly induced after salt and high osmotic stress treatments; the over-expression of SbPIP2 in tobacco also enhanced the salt and high osmotic stress tolerance of transgenic plants [76]. We previously cloned two PIP s ( CrPIP1;5 and CrPIP2;3 ) from another tropical halophyte, Canavalia rosea [ 40 , 41 ], and performed transgenic overexpression assays in Arabidopsis. Unexpectedly, CrPIP2;3 enhanced the drought and salt tolerance of transgenic Arabidopsis, while CrPIP1;5 overexpression caused obviously higher drought and salt sensitivity in Arabidopsis plants, although their heterologous expression both caused slight sensitivities to high osmotic and salt stress in yeast cells. In this study, eight TtAQPs showed different tolerance or sensitivity changes to high salinity, osmotic stress (mimicking drought), and heat treatments in yeast (Figs. 9 and 10 ), and their tolerance to H 2 O 2 also showed obvious differences (Figs. 10 A and S4B). This is inconsistent with our previous research on plant PIP gene expression in yeast [ 40 , 41 ], further indicating that the different plant species’ AQPs might act in member-specific ways to regulate water or other small molecules or the interaction with other protein members and thereby playing specific roles in plants’ abiotic stress responses. Further deep and systematic research on TtAQP s for abiotic stress-resistant genetic improvement in other microorganisms or crops is still required. Conclusions This study presents the first genome-wide characterization of the AQP family in T. tetragonoides and possible molecular mechanisms, identifying 58 TtAQPs genes based on the genome sequencing data/through comprehensive genomic analysis. Through systematic analyses of evolutionary relationships, gene structure, gene duplication, promoters, and the possible regulatory factors of TtAQP s, we revealed differential expression patterns among TtAQP subfamilies, with TtPIPs and TtTIPs exhibiting particularly distinct regulatory profiles, suggesting their specialized functional roles in plant development and environmental stress responses of T. tetragonoides . Functional validation through heterologous expression in different yeast strains demonstrated the potential involvement of selected eight candidate TtAQP s in stress response mechanisms. These finding provide a foundational framework for understanding the AQP -mediated stress adaptation in T. tetragonoides and offer valuable insights for future genetic improvement application of TtAQP s at enhancing stress tolerance in other plant species and microorganisms. Abbreviations AQP Aquaporin MIP Major intrinsic protein CE Cis-acting element RT-PCR Reverse transcription-polymerase chain reaction PIP Plasma membrane intrinsic protein TIP Tonoplast intrinsic protein NIP NOD26-like intrinsic protein SIP Small basic intrinsic protein XIP Uncategorized X intrinsic protein TF Transcription factor WGD Whole genome duplication TD Tandem duplication PD Proximal duplication TRD Transposed duplication DD Dispersed duplication FPKM Fragments per kilobase of transcript per million mapped reads Declarations Supplementary Material The online version contains supplementary material available at https://doi.org/... Acknowledgment Not applicable. Author’s contributions Lisha Cao: Data curation, Writing-original draft, Investigation. Fuying Xie: Data curation, Investigation. Lihua Chen: Investigation. Zhengfeng Wang: Data curation. Mei Zhang and Shuguang Jian: Writing-review and editing. Funding This research was funded by the Guangdong Science and Technology Program (No. 2024B1212050007), and the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (No. GML2019ZD0408). These funders had no roles in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Data availability All data generated or analyzed during this study are included in this published article [and its supplementary information file]. Ethics approval and consent to participate No particular approvals were necessary; materials were gathered in accordance with the national rules and under the supervision and consent of South China National Botanical Garden (SCNBG), Guangzhou, China, and all authors adhered to all local, national, and international guidelines and legislation in accordance with the rules established by SCNBG. Consent for publication Not applicable. Competing interests The authors declare no competing interests. References Atzori G, Nissim W, Macchiavelli T, Vita F, Azzarello E, Pandolfi C, Masi E, Stefano Mancuso S. Tetragonia tetragonioides (pallas) kuntz. as promising salt-tolerant crop in a saline agricultural context. Agr Water Manage. 2020;240:106261. Lai XK, Lin NX, Chen JZ, Huang L, Ji JF, Chen RH, Liang WQ, Wu JX. Studies on cultivation trial of salt-tolerant New Zealand spinach in Quanzhou bay (In Chinese). Fujian Agri Sci Tech. 2016;1:24–7. Li RX, Nie WJ, Li B, Li JL, Wang XY, Qiao P, Fu R. Purification effects of Tetragonia tetragonoides on seawater with different salinity and eutrophication degrees (In Chinese). Shandong Agri Sci. 2022;54(3):99–105. Choi HS, Cho JY, Kim SJ, Ham KS, Moon JH. New lignan tyramide, phenolics, megastigmanes, and their glucosides from aerial parts of New Zealand spinach, Tetragonia tetragonoides . Food Sci Biotechnol. 2019;29(5):599–608. Chen X. Why are there sudden instances of seawater inundation in many places in China[N]. Sci Technol Dly. 2024-10–31. Xu WF. Researches on recovery the seawater-intrusion fields & plant characteristics under seawater stress of Mesembryanthemum crystallinum L. Hainan: Hainan University. 2018;4–6. Neves MA, Miguel MG, Panagapoulos T, Beltrao J. Salt removing species-an environmentally safe and clean technique to control salinity[M]. Salt Removing Species. 2014;1–11. Wilson C, Lesch SM, Grieve CM. Growth stage modulates salinity tolerance of New Zealand spinach ( Tetragonia tetragonioides Pall.) and red orach ( Atriplex hortensis L). Ann Bot. 2000;85:501–9. Yousif BS, Nguyen NT, Fukuda Y, Hakata H, Okamoto Y, Masaoka Y, Saneoka H. Effect of salinity on growth, mineral composition, photosynthesis and water relations of two vegetable crops; New Zealand spinach ( Tetragonia tetragonioides ) and water spinach ( Ipomoea aquatica ). Int J Agric Biol. 2010;12:211–6. Qin S, Liu Y, Han Y, Xu G, Wan S, Cui F, Li G. Aquaporins and their function in root water transport under salt stress conditions in Eutrema salsugineum . Plant Sci. 2019;287:110199. Nongpiur RC, Singla-Pareek SL, Pareek A. The quest for osmosensors in plants. J Exp Bot. 2020;71(2):595–607. Byrt CS, Zhang RY, Magrath I, Chan KX, De Rosa A, McGaughey S. Exploring aquaporin functions during changes in leaf water potential. Front Plant Sci. 2023;14:1213454. Maurel C, Tournaire-Roux C, Verdoucq L, Santoni V. Hormonal and environmental signaling pathways target membrane water transport. Plant Physiol. 2021;187(4):2056–70. Ishibashi K, Kondo S, Hara S, Morishita Y. The evolutionary aspects of aquaporin family. Am J Physiol Regul Integr Comp Physiol. 2011;300(3):R566–76. Kaldenhoff R, Ribas-Carbo M, Sans JF, Lovisolo C, Heckwolf M, Uehlein N. Aquaporins and plant water balance. Plant Cell Environ. 2008;31(5):658–66. Mukherjee S, Roy S, Corpas FJ. Aquaporins: a vital nexus in H 2 O 2 -gasotransmitter signaling. Trends Plant Sci. 2024;29(6):681–93. Takano J, Wada M, Ludewig U, Schaaf G, von Wirén N, Fujiwara T. The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation. Plant Cell. 2006;18:1498–509. Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M. A silicon transporter in rice. Nature. 2006;440:688–91. Bienert GP, Thorsen M, Schüssler MD, Nilsson HR, Wagner A, Tamás MJ, Jahn TP. A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH) 3 and Sb(OH) 3 across membranes. BMC Biol. 2008;6:26. Zhao XQ, Mitani NM, Yamaji N, Shen RS, Ma JF. Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice. Plant Physiol. 2010;153:1871–7. Mitani-Ueno N, Yamaji N, Zhao FJ, Ma JF. The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic. J Exp Bot. 2011;62:4391–8. Srivastava AK, Penna S, Nguyen DV, Tran LS. Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses. Crit Rev Biotechnol. 2016;36(3):389–98. Du B, Franzisky BL, Muhammad W, Alfarraj S, Geilfus CM, Rennenberg H. How to cope with stress in the desert-the date palm approach. Plant Cell Environ. 2025;48(1):768–80. Tarolli P, Luo J, Park E, Barcaccia G, Masin R. Soil salinization in agriculture: mitigation and adaptation strategies combining nature-based solutions and bioengineering. iScience. 2024;27(2):108830. Zargar SM, Nagar P, Deshmukh R, Nazir M, Wani AA, Masoodi KZ, Agrawal GK, Rakwal R. Aquaporins as potential drought tolerance inducing proteins: towards instigating stress tolerance. J Proteom. 2017;169:233–8. Martinez-Ballesta Mdel C, Carvajal M. New challenges in plant aquaporin biotechnology. Plant Sci. 2014;217–218:71–7. Jang JY, Lee SH, Rhee JY, Chung GC, Ahn SJ, Kang H. Transgenic Arabidopsis and tobacco plants overexpressing an aquaporin respond differently to various abiotic stresses. Plant Mol Biol. 2007;64(6):621–32. Zhou L, Wang C, Liu R, Han Q, Vandeleur RK, Du J, Tyerman S, Shou H. Constitutive overexpression of soybean plasma membrane intrinsic protein GmPIP1;6 confers salt tolerance. BMC Plant Biol. 2014;14:181. Sreedharan S, Shekhawat UK, Ganapathi TR. Transgenic banana plants overexpressing a native plasma membrane aquaporin MusaPIP1;2 display high tolerance levels to different abiotic stresses. Plant Biotechnol J. 2013;11(8):942–52. Sreedharan S, Shekhawat UK, Ganapathi TR. Constitutive and stress-inducible overexpression of a native aquaporin gene ( MusaPIP2;6 ) in transgenic banana plants signals its pivotal role in salt tolerance. Plant Mol Biol. 2015;88(1–2):41–52. Alavilli H, Awasthi JP, Rout GR, Sahoo L, Lee BH, Panda SK. Overexpression of a barley aquaporin gene, HvPIP2;5 confers salt and osmotic stress tolerance in yeast and plants. Front Plant Sci. 2016;7:1566. Li R, Wang J, Li S, Zhang L, Qi C, Weeda S, Zhao B, Ren S, Guo YD. Plasma membrane intrinsic proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 conferring enhanced drought stress tolerance in tomato. Sci Rep. 2016;6:31814. Wang L, Li QT, Lei Q, Feng C, Zheng X, Zhou F, Li L, Liu X, Wang Z, Kong J. Ectopically expressing MdPIP1;3 , an aquaporin gene, increased fruit size and enhanced drought tolerance of transgenic tomatoes. BMC Plant Biol. 2017;17(1):246. Martins CP, Neves DM, Cidade LC, Mendes AF, Silva DC, Almeida AF, Coelho-Filho MA, Gesteira AS, Soares-Filho WS, Costa MG. Expression of the citrus CsTIP2;1 gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions. Planta. 2017;245(5):951–63. Ayadi M, Brini F, Masmoudi K. Overexpression of a wheat aquaporin gene, TdPIP2;1 , enhances salt and drought tolerance in transgenic durum wheat cv. Maali. Int J Mol Sci. 2019;20(10):2389. Wang Y, Zhang Y, An Y, Wu J, He S, Sun L, Hao F. Wheat TaTIP4;1 confers enhanced tolerance to drought, salt and osmotic stress in Arabidopsis and rice. Int J Mol Sci. 2022;23(4):2085. Wang X, Li Y, Ji W, Bai X, Cai H, Zhu D, Sun XL, Chen LJ, Zhu YM. A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana . J Plant Physiol. 2011;168(11):1241–8. Wang X, Cai H, Li Y, Zhu Y, Ji W, Bai X, Zhu D, Sun X. Ectopic overexpression of a novel Glycine soja stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic Arabidopsis thaliana plants. J Plant Res. 2015;128(1):103–13. Li J, Yu G, Sun X, Liu Y, Liu J, Zhang X, Jia C, Pan H. AcPIP2, a plasma membrane intrinsic protein from halophyte Atriplex canescens , enhances plant growth rate and abiotic stress tolerance when overexpressed in Arabidopsis thaliana . Plant Cell Rep. 2015;34(8):1401–15. Lin R, Zheng J, Pu L, Wang Z, Mei Q, Zhang M, Jian S. Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress. BMC Plant Biol. 2021;21(1):333. Zheng J, Lin R, Pu L, Wang Z, Mei Q, Zhang M, Jian S. Ectopic expression of CrPIP2;3 , a plasma membrane intrinsic protein gene from the halophyte Canavalia rosea , enhances drought and salt-alkali stress tolerance in Arabidopsis. Int J Mol Sci. 2021;22(2):565. Shekoofa A, Sinclair TR. Aquaporin activity to improve crop drought tolerance. Cells. 2018;7(9):123. Qian W, Yang X, Li J, Luo R, Yan X, Pang Q. Genome-wide characterization and expression analysis of aquaporins in salt cress ( Eutrema salsugineum ). PeerJ. 2019;7:e7664. Faize M, Fumanal B, Luque F, Ramírez-Tejero JA, Zou Z, Qiao X, Faize L, Gousset-Dupont A, Roeckel-Drevet P, Label P, Venisse JS. Genome wild analysis and molecular understanding of the aquaporin diversity in olive trees ( Olea Europaea L). Int J Mol Sci. 2020;21(11):4183. Kumar N, Kumawat S, Khatri P, Singla P, Tandon G, Bhatt V, Shinde S, Patil GB, Sonah H, Deshmukh R. Understanding aquaporin transport system in highly stress-tolerant and medicinal plant species Jujube ( Ziziphus jujuba Mill). J Biotechnol. 2020;324:103–11. Grondin A, Affortit P, Tranchant-Dubreuil C, de la Fuente-Cantó C, Mariac C, Gantet P, Vadez V, Vigouroux Y, Laplaze L. Aquaporins are main contributors to root hydraulic conductivity in pearl millet [ Pennisetum glaucum (L) R. Br]. PLoS ONE. 2020;15(10):e0233481. Zhang GH, Yu ZM, Teixeira da Silva JA, Wen DZ. Identification of aquaporin members in Acacia auriculiformis and functional characterization of AaPIP1-2 involved in drought stress. Environ Exp Bot. 2021;185:104425. He W, Liu M, Qin X, Liang A, Chen Y, Yin Y, Qin K, Mu Z. Genome-wide identification and expression analysis of the aquaporin gene family in Lycium barbarum during fruit ripening and seedling response to heat stress. Curr Issues Mol Biol. 2022;44(12):5933–48. Guo Z, Ma D, Li J, Wei M, Zhang L, Zhou L, Zhou X, He S, Wang L, Shen Y, Li QQ, Zheng HL. Genome-wide identification and characterization of aquaporins in mangrove plant Kandelia obovata and its role in response to the intertidal environment. Plant Cell Environ. 2022;45(6):1698–718. Guo Z, Wei M, Xu C, Wang L, Li J, Liu J, Zhong Y, Chi B, Song S, Zhang L, Song L, Ma D, Zheng HL. Genome-wide identification of Avicennia marina aquaporins reveals their role in adaptation to intertidal habitats and their relevance to salt secretion and vivipary. Plant Cell Environ. 2024;47(3):832–53. Ndayambaza B, Si J, Zhou D, Bai X, Jia B, He X, Wang C, Qin J, Zhu X, Liu Z, Wang B. Genome-wide analysis of aquaporins gene family in Populus euphratica and its expression patterns in response to drought, salt stress, and phytohormones. Int J Mol Sci. 2024;25(18):10185. Huang Z, Ding Q, Wang Z, Jian S, Zhang M. Genome-wide identification and expression analyses of the thaumatin-like protein gene family in Tetragonia tetragonoides (Pall.) Kuntze reveal their functions in abiotic stress responses. Plants (Basel). 2024;13(17):2355. Chen C, Chen H, Zhang Y, Thomas HR, Frank MH, He Y, Xia R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant. 2020;13(8):1194–202. Nguyen MX, Moon S, Jung KH. Genome-wide expression analysis of rice aquaporin genes and development of a functional gene network mediated by aquaporin expression in roots. Planta. 2013;238(4):669–81. Nei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986;3(5):418–26. Maruyama K, Ogata T, Kanamori N, Yoshiwara K, Goto S, Yamamoto YY, Tokoro Y, Noda C, Takaki Y, Urawa H, Iuchi S, Urano K, Yoshida T, Sakurai T, Kojima M, Sakakibara H, Shinozaki K, Yamaguchi-Shinozaki K. Design of an optimal promoter involved in the heat-induced transcriptional pathway in Arabidopsis, soybean, rice and maize. Plant J. 2017;89(4):671–80. Liu H, Ding Q, Cao L, Huang Z, Wang Z, Zhang M, Jian S. Identification of the Abscisic acid-, Stress-, and Ripening-Induced ( ASR ) family involved in the adaptation of Tetragonia tetragonoides (Pall.) Kuntze to saline-alkaline and drought habitats. Int J Mol Sci. 2023;24(21):15815. Offringa R, Huang F. Phosphorylation-dependent trafficking of plasma membrane proteins in animal and plant cells. J Integr Plant Biol. 2013;55(9):789–808. Ahmed S, Kouser S, Asgher M, Gandhi SG. Plant aquaporins: a frontward to make crop plants drought resistant. Physiol Plant. 2021;172(2):1089–105. Wu HJ, Zhang Z, Wang JY, Oh DH, Dassanayake M, Liu B, Huang Q, Sun HX, Xia R, Wu Y, Wang YN, Yang Z, Liu Y, Zhang W, Zhang H, Chu J, Yan C, Fang S, Zhang J, Wang Y, Zhang F, Wang G, Lee SY, Cheeseman JM, Yang B, Li B, Min J, Yang L, Wang J, Chu C, Chen SY, Bohnert HJ, Zhu JK, Wang XJ, Xie Q. Insights into salt tolerance from the genome of Thellungiella salsuginea . Proc Natl Acad Sci USA. 2012;109(30):12219–24. Golldack D, Li C, Mohan H, Probst N. Tolerance to drought and salt stress in plants: unraveling the signaling networks. Front Plant Sci. 2014;5:151. Maurel C, Boursiac Y, Luu DT, Santoni V, Shahzad Z, Verdoucq L. Aquaporins in plants. Physiol Rev. 2015;95(4):1321–58. Groszmann M, De Rosa A, Chen W, Qiu J, McGaughey SA, Byrt CS, Evans JR. A high-throughput yeast approach to characterize aquaporin permeabilities: profiling the Arabidopsis PIP aquaporin sub-family. Front Plant Sci. 2023;14:1078220. Cuéllar-Cruz M, Briones-Martin-del-Campo M, Cañas-Villamar I, Montalvo-Arredondo J, Riego-Ruiz L, Castaño I, De Las Peñas A. High resistance to oxidative stress in the fungal pathogen Candida glabrata is mediated by a single catalase, Cta1p, and is controlled by the transcription factors Yap1p, Skn7p, Msn2p, and Msn4p. Eukaryot Cell. 2008;7(5):814–25. Zhang M, Wang Z, Jian S. Functional characterization of heat shock factor ( CrHsf ) families provide comprehensive insight into the adaptive mechanisms of Canavalia rosea (Sw.) DC. to tropical coral islands. Int J Mol Sci. 2022;23(20):12357. Shabala S, Bose J, Hedrich R. Salt bladders: do they matter? Trends Plant Sci. 2014;19(11):687–91. Guo H, Zhang L, Cui YN, Wang SM, Bao AK. Identification of candidate genes related to salt tolerance of the secretohalophyte Atriplex canescens by transcriptomic analysis. BMC Plant Biol. 2019;19(1):213. Gómez-Méndez MF, Amezcua-Romero JC, Rosas-Santiago P, Hernández-Domínguez EE, de Luna-Valdez LA, Ruiz-Salas JL, Vera-Estrella R, Pantoja O. Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress. Plant Physiol. 2023;191(1):199–218. Sharma R, Kumar D, Parkirti P, Singh A, Sharma A, Langeh K, Singh A, Sharma M, Mir NR, Kapoor A, Bhardwaj N, Ohri R. Membrane transporters in plants: key players in abiotic and biotic stress tolerance and nutritional transport. Plant Physiol Bioch. 2025;227:110084. Dahuja A, Kumar RR, Sakhare A, Watts A, Singh B, Goswami S, Sachdev A, Praveen S. Role of ATP-binding cassette transporters in maintaining plant homeostasis under abiotic and biotic stresses. Physiol Plant. 2021;171(4):785–801. Sutka M, Li G, Boudet J, Boursiac Y, Doumas P, Maurel C. Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions. Plant Physiol. 2011;155(3):1264–76. Chang W, Liu X, Zhu J, Fan W, Zhang Z. An aquaporin gene from halophyte Sesuvium portulacastrum , SpAQP1 , increases salt tolerance in transgenic tobacco. Plant Cell Rep. 2016;35(2):385–95. Chen Q, Yang S, Kong X, Wang C, Xiang N, Yang Y, Yang Y. Molecular cloning of a plasma membrane aquaporin in Stipa purpurea , and exploration of its role in drought stress tolerance. Gene. 2018;665:41–8. Wang X, Gao F, Bing J, Sun W, Feng X, Ma X, Zhou Y, Zhang G. Overexpression of the jojoba aquaporin gene, ScPIP1 , enhances drought and salt tolerance in transgenic Arabidopsis. Int J Mol Sci. 2019;20(1):153. Patel J, Khatri K, Khandwal D, Gupta NK, Choudhary B, Hapani D, Koshiya J, Syed SN, Phillips DW, Jones HD, Mishra A. Modulation of physio-biochemical and photosynthesis parameters by overexpressing SbPIP2 gene improved abiotic stress tolerance of transgenic tobacco. Physiol Plant. 2024;176(3):e14384. Additional Declarations No competing interests reported. Supplementary Files SupplementaryTableS1.xlsx SupplementaryTableS2.xlsx SupplementaryTableS3.xlsx SupplementaryTableS4.xlsx SupplementaryTableS5.xlsx SupplementarymaterialofTtAQPMs.docx Cite Share Download PDF Status: Published Journal Publication published 31 Oct, 2025 Read the published version in BMC Plant Biology → Version 1 posted Editorial decision: Revision requested 18 Aug, 2025 Reviews received at journal 14 Aug, 2025 Reviews received at journal 11 Aug, 2025 Reviewers agreed at journal 31 Jul, 2025 Reviewers agreed at journal 31 Jul, 2025 Reviewers agreed at journal 28 Jul, 2025 Reviewers invited by journal 28 Jul, 2025 Editor assigned by journal 28 Jul, 2025 Editor invited by journal 23 Jul, 2025 Submission checks completed at journal 22 Jul, 2025 First submitted to journal 22 Jul, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7136705","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":492276124,"identity":"e29688fb-cbad-4694-a9f2-5e45cfb7318e","order_by":0,"name":"Lisha Cao","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Lisha","middleName":"","lastName":"Cao","suffix":""},{"id":492276125,"identity":"7c808734-e668-4841-8203-74e6c9a1f230","order_by":1,"name":"Fuying Xie","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Fuying","middleName":"","lastName":"Xie","suffix":""},{"id":492276129,"identity":"4a3dd3e2-fe9e-4c00-a87d-78b8d66ac63e","order_by":2,"name":"Lihua Chen","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Lihua","middleName":"","lastName":"Chen","suffix":""},{"id":492276131,"identity":"df97ec82-b14e-4bb9-90d9-15688cea33d9","order_by":3,"name":"Zhengfeng Wang","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Zhengfeng","middleName":"","lastName":"Wang","suffix":""},{"id":492276133,"identity":"597f47b1-0004-48df-889d-0a2b9ef00034","order_by":4,"name":"Mei Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1ElEQVRIiWNgGAWjYDACCQjFw8/AwEaiFskGUrUwGBwgVov87OZnD7/m2MgYnz/87OEXBjt5BvazB/BqYZxzzNxYdlsaj9mNNHNjGYZkwwaevAS8WpglEsykJbcdBmrhYZOWYGBOYJDgMcCrhU0i/RtQy38e4/4zIC31hLXwSOSYSX7cdgCoLIdN8gPDYcJaJCRyyqQZtyXzSID8wmBw3LCNJwe/FvkZ6dskf26zs+fvB4bYj4pqeX72M/i1gAAzD5xhQGQaYPyBzhgFo2AUjIJRgAwA0Pg4kTxt6CIAAAAASUVORK5CYII=","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":true,"prefix":"","firstName":"Mei","middleName":"","lastName":"Zhang","suffix":""},{"id":492276136,"identity":"ae64c1b3-6e30-4db3-b7da-c3417246555c","order_by":5,"name":"Shuguang Jian","email":"","orcid":"","institution":"Chinese Academy of Sciences","correspondingAuthor":false,"prefix":"","firstName":"Shuguang","middleName":"","lastName":"Jian","suffix":""}],"badges":[],"createdAt":"2025-07-16 06:53:37","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7136705/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7136705/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s12870-025-07410-z","type":"published","date":"2025-10-31T15:58:39+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":87925834,"identity":"e00d3f13-a63f-4f5b-99f5-ffaded6f2862","added_by":"auto","created_at":"2025-07-30 12:35:46","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":4016958,"visible":true,"origin":"","legend":"\u003cp\u003ePhylogenetic relationships of the 58 TtAQPs from \u003cem\u003eTetragonia tetragonoides\u003c/em\u003e, 37 CrAQPs from \u003cem\u003eCanavalia rosea\u003c/em\u003e, 35 AtAQPs from Arabidopsis, and 34 OsAQPs from rice. The phylogenetic tree was constructed using MEGA X software, with ClustalW alignment, maximum likelihood (ML) method, the bootstrap method, and 1000 repetitions. All five subfamilies of the AQP gene family are well separated in different clades and represented by different background colors.\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/3a75bfc0dd3d873995fab0f3.jpg"},{"id":87926817,"identity":"fdd6e2ed-998e-4ac7-89fe-a1d573df72bd","added_by":"auto","created_at":"2025-07-30 12:43:46","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":1036580,"visible":true,"origin":"","legend":"\u003cp\u003eLocations of the 58 \u003cem\u003eTtAQPs\u003c/em\u003e on 16 chromosomes of \u003cem\u003eT. tetragonoides\u003c/em\u003e.\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/f0d3311aba3212a568214fc7.jpg"},{"id":87925831,"identity":"b9bfced6-f6ee-4fbd-a2b0-5b965ddca452","added_by":"auto","created_at":"2025-07-30 12:35:45","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":790432,"visible":true,"origin":"","legend":"\u003cp\u003ePhylogenetic relationships, gene structure, and motif compositions of the TtAQP proteins or genes. \u003cstrong\u003e(A) \u003c/strong\u003eThe phylogenetic tree on the left side was constructed using MEGA X. The five major groups are marked with different background colors. \u003cstrong\u003e(B) \u003c/strong\u003eThe exon–intron organization of the \u003cem\u003eTtAQPs\u003c/em\u003ewas constructed using GSDS 2.0 (https://gsds.gao-lab.org/). \u003cstrong\u003e(C) \u003c/strong\u003eThe conserved motifs of each group on the right side were identified using the MEME web server (http://meme-suite.org/tools/meme). Different motifs are represented by different colored boxes.\u003c/p\u003e","description":"","filename":"Figure3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/5cec2d070bc2d88745a7f396.jpg"},{"id":87925828,"identity":"906e5cb3-8b0a-447e-a567-62968bf113cd","added_by":"auto","created_at":"2025-07-30 12:35:45","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":4269125,"visible":true,"origin":"","legend":"\u003cp\u003eChromosomal distribution and intragroup covariance analysis of the \u003cem\u003eTtAQPs\u003c/em\u003e. The gray section represents the collinearity of all genes within \u003cem\u003eT. tetragonoides\u003c/em\u003e; the green line represents the collinearity within the \u003cem\u003eTtAQP\u003c/em\u003e gene family.\u003c/p\u003e","description":"","filename":"Figure4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/cbdf3650b5828d169eb4f53e.jpg"},{"id":87926814,"identity":"5f618357-6fc6-4ca8-bf0a-b7ca9106625a","added_by":"auto","created_at":"2025-07-30 12:43:46","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":1716166,"visible":true,"origin":"","legend":"\u003cp\u003eNumbers and distribution of the \u003cem\u003ecis\u003c/em\u003e-acting elements (CEs) in the promoter regions of 58 \u003cem\u003eTtAQPs\u003c/em\u003e. The 16 CEs (MYB, ABRE, MYC, ARE, HSE, TGACG-motif, ERE, CGTCA-motif, TC-rich repeat, WUN-motif, TCA element, TATC box, MBS, AuxRR-core, TGA-element, and P-box) are labeled with different colored symbols in predicted promoter regions. The scale bar represents 200 bp.\u003c/p\u003e","description":"","filename":"Figure5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/26e37da98330292d0b50a4b8.jpg"},{"id":87926821,"identity":"455ccd71-7bd3-4a96-bb41-3640e5ed582a","added_by":"auto","created_at":"2025-07-30 12:43:46","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":751612,"visible":true,"origin":"","legend":"\u003cp\u003eHeatmaps showing the expression levels of the 58 \u003cem\u003eTtAQPs\u003c/em\u003e in the roots, stems, leaves, flower buds, and young fruit of \u003cem\u003eT. tetragonoides\u003c/em\u003e plants.\u003c/p\u003e","description":"","filename":"Figure6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/25520abfed9d82fd0bff0c57.jpg"},{"id":87926819,"identity":"76d8089a-9621-45b1-96ec-0250717802c1","added_by":"auto","created_at":"2025-07-30 12:43:46","extension":"jpg","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":2774016,"visible":true,"origin":"","legend":"\u003cp\u003eHeatmaps showing expression differences in 58 \u003cem\u003eTtAQPs\u003c/em\u003ein mature \u003cem\u003eT\u003c/em\u003e. \u003cem\u003etetragonoides\u003c/em\u003e roots \u003cstrong\u003e(A)\u003c/strong\u003e, stems\u003cstrong\u003e (B)\u003c/strong\u003e, and leaves\u003cstrong\u003e (C)\u003c/strong\u003e.\u003c/p\u003e","description":"","filename":"Figure7.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/5560173cadaf9e9ae0c02399.jpg"},{"id":87925848,"identity":"58b58dcf-498c-443f-b328-741dc75ec845","added_by":"auto","created_at":"2025-07-30 12:35:46","extension":"jpg","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":1605212,"visible":true,"origin":"","legend":"\u003cp\u003eQuantitative RT-PCR detection of the expression levels of the 14 \u003cem\u003eTtAQPs\u003c/em\u003e responding to different stressors (600 mM NaCl for salt, 150 mM NaHCO\u003csub\u003e3\u003c/sub\u003e for alkali, 300 mM mannitol for artificial drought, and 45℃ for heat) in \u003cem\u003eT. tetragonoides\u003c/em\u003e seedling plants: \u003cstrong\u003e(A)\u003c/strong\u003e six \u003cem\u003eTtPIPs\u003c/em\u003e; (B) eight other TtAQPs, including six \u003cem\u003eTtTIPs\u003c/em\u003e and two \u003cem\u003eTtSIPs\u003c/em\u003e.\u003cstrong\u003e (B)\u003c/strong\u003e High osmotic (sorbitol) stress tolerance confirmation in yeast. \u003cstrong\u003e(C)\u003c/strong\u003e Heat (50°C) stress tolerance confirmation in yeast.\u003c/p\u003e","description":"","filename":"Figure8.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/f7a29f625eeca00219da64d1.jpg"},{"id":87927327,"identity":"3fe1f6c6-6484-40d9-a985-0d6b261c4901","added_by":"auto","created_at":"2025-07-30 12:51:47","extension":"jpg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":634000,"visible":true,"origin":"","legend":"\u003cp\u003eThe tolerance confirmations of the 3 \u003cem\u003eTtTIPs\u003c/em\u003e(\u003cem\u003eTtTIP1;2\u003c/em\u003e, \u003cem\u003eTtTIP1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e), 3 \u003cem\u003eTtPIPs\u003c/em\u003e (\u003cem\u003eTtPIP1;1\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;7\u003c/em\u003e), \u003cem\u003eTtNIP1;2\u003c/em\u003e, and \u003cem\u003eTtSIP1;2\u003c/em\u003eheteroexpression in wild type (WT) yeast. \u003cstrong\u003e(A)\u003c/strong\u003e Salt (NaCl) stress tolerance confirmations in yeast; \u003cstrong\u003e(B)\u003c/strong\u003e High osmotic (sorbitol) stress tolerance confirmations in yeast; and \u003cstrong\u003e(C)\u003c/strong\u003e Heat (50°C) stress tolerance confirmations in yeast.\u003c/p\u003e","description":"","filename":"Figure9.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/0db186fcc25d865e37933e8b.jpg"},{"id":87925836,"identity":"e5d8524d-adc3-4317-8699-173bcc67cb1c","added_by":"auto","created_at":"2025-07-30 12:35:46","extension":"jpg","order_by":10,"title":"Figure 10","display":"","copyAsset":false,"role":"figure","size":625167,"visible":true,"origin":"","legend":"\u003cp\u003eTolerance confirmation based on the heteroexpression of eight \u003cem\u003eTtAQPs\u003c/em\u003e in H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-sensitive mutant strain (\u003cem\u003eskn7Δ\u003c/em\u003e) yeast. \u003cstrong\u003e(A)\u003c/strong\u003e Oxidative stress (H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) tolerance confirmation in \u003cem\u003eskn7Δ\u003c/em\u003e. \u003cstrong\u003e(B)\u003c/strong\u003e Heat (52°C) stress tolerance confirmation in \u003cem\u003eskn7Δ\u003c/em\u003e.\u003c/p\u003e","description":"","filename":"Figure10.jpg","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/fb15288c4bab8d6fbd0b1c30.jpg"},{"id":95040418,"identity":"52d226ab-584e-46bb-a971-0ececfb05723","added_by":"auto","created_at":"2025-11-03 16:08:26","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":20772551,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/dbc77066-1a17-45af-ab97-1c06d197b673.pdf"},{"id":87926810,"identity":"2363efc6-8c15-48f6-88c6-b3e3939dd159","added_by":"auto","created_at":"2025-07-30 12:43:45","extension":"xlsx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":185631,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryTableS1.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/79d30977514fcc623598650d.xlsx"},{"id":87925837,"identity":"34fb8df7-86ac-4a60-a125-101426346122","added_by":"auto","created_at":"2025-07-30 12:35:46","extension":"xlsx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":12494,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryTableS2.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/1a591160cdbff185c93c3f5c.xlsx"},{"id":87925826,"identity":"ab6eeff6-1912-498e-8da2-97fb8f9813f4","added_by":"auto","created_at":"2025-07-30 12:35:45","extension":"xlsx","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":26486,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryTableS3.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/1bb92441eeb0fbaf88208ab5.xlsx"},{"id":87926812,"identity":"dc7079cc-aed0-43c8-9a7e-67afdd507056","added_by":"auto","created_at":"2025-07-30 12:43:46","extension":"xlsx","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":29739,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryTableS4.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/e17dbd601ee5911da32a42ea.xlsx"},{"id":87925887,"identity":"5e2471aa-01f1-45e5-bcee-b5544e3b0782","added_by":"auto","created_at":"2025-07-30 12:35:47","extension":"xlsx","order_by":4,"title":"","display":"","copyAsset":false,"role":"supplement","size":145219,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementaryTableS5.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/d0e4c094d1ac9fcbccad8beb.xlsx"},{"id":87925862,"identity":"fac17a4b-781b-4769-889d-97c4d0e4a11e","added_by":"auto","created_at":"2025-07-30 12:35:47","extension":"docx","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":4925110,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementarymaterialofTtAQPMs.docx","url":"https://assets-eu.researchsquare.com/files/rs-7136705/v1/bc5bb902f95de3287ec2e97a.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Molecular characterization of Tetragonia tetragonoides (Pall.) aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress","fulltext":[{"header":"Background","content":"\u003cp\u003e\u003cem\u003eTetragonia tetragonoides\u003c/em\u003e (Pall.) Kuntze is a calcium-rich vegetable with substantial commercial value for human health. \u003cem\u003eT. tetragonoides\u003c/em\u003e is widely distributed in the coastal areas of tropical and subtropical regions, and it has gradually emerged as a novel coastal cash crop both for the farm economy and for the improvement of coastal saline soils [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. This species is adaptable to multiple extreme stressors, including heat, drought, sea waterlogging, and high salinity\u0026ndash;alkalinity, and it grows widely in the tropical coastal zones, including beach sand, mangrove fringe, islands, and reefs [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. The suitable salinity range for regular growth of \u003cem\u003eT. tetragonoides\u003c/em\u003e is below 2.5% (NaCl); thus, its life cycle can also be completed normally in seawater irrigation areas, which means that this species could be developed as a potential plant for perennial saltwater intrusion areas [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. Furthermore, \u003cem\u003eT. tetragonoides\u003c/em\u003e has a high nutritional value and economic value due to its high content of inorganic salts (such as calcium (Ca), sodium (Na), and potassium (K)) and several bioactive compounds, including cerebrosides, steryl glucosides, diterpenes, flavonol glycosides, and lignan amides, which make it a feature seawater vegetable with wide cultivation [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]. In recent years, saltwater intrusion has constrained the development of marine agriculture [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Therefore, the exploration and promotion of salt-tolerant crops play an important role in marine agriculture development [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e].\u003c/p\u003e\u003cp\u003e\u003cem\u003eT. tetragonioides\u003c/em\u003e is an annual or biennial endo-secretohalophyte with salt bladders in the epidermal cells of its leaves and stems; thus, this species can be used as a salt-removing plant due to its high Na\u003csup\u003e+\u003c/sup\u003e and K uptake efficiency [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Considering this, \u003cem\u003eT. tetragonioides\u003c/em\u003e can withstand an electrical conductivity (EC) in the growing medium as high as 10 dS m\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e (about 100 mM NaCl) and can complete its life cycle normally [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e, \u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e]. Some reports have indicated that salinity levels of 50\u0026ndash;100 mM NaCl (EC 5\u0026ndash;10 dS m\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e) can result in a salt-induced growth response in \u003cem\u003eT. tetragonioides\u003c/em\u003e [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. In its native habitats, the increased water use efficiency (WUE) in \u003cem\u003eT. tetragonioides\u003c/em\u003e caused by seawater irrigation is a key influencing factor for its growth and development [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e], which results in high hydraulic conductivity, which likely involves aquaporins (AQPs), similar to another model halophyte species, \u003cem\u003eEutrema salsugineum\u003c/em\u003e [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. The related osmotic stress caused by multiple stress substances and environmental challenges \u003cem\u003ein vivo\u003c/em\u003e and \u003cem\u003ein vitro\u003c/em\u003e must be relieved by osmosensors, and therefore, AQPs must act as master regulators of the osmotic stress response [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eAQPs are also called water channel proteins, belonging to the major intrinsic protein (MIP) superfamily and being small membrane proteins (21\u0026ndash;34 kD). AQPs mainly function in regulating cellular water transport [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. Plant AQPs can be subdivided into five major groups based on their different subcellular or plant organ localizations: plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs; which target the endoplasmic reticulum), and uncategorized X intrinsic proteins (XIPs) [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]. The PIP subfamily can be further subdivided into two groups: PIP1and PIP2 [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. Plant PIPs present dual localization in the plasma membrane (PM) and chloroplast envelope [\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e, \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. In addition to water molecules, AQPs also facilitate the transport of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e, gasotransmitters (including NO), H\u003csub\u003e2\u003c/sub\u003eS, ammonia (NH\u003csub\u003e3\u003c/sub\u003e), carbon dioxide (CO\u003csub\u003e2\u003c/sub\u003e), and other uncharged solutes across cell membranes [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e], but some studies have demonstrated that plant AQPs (mainly NIPs) also facilitate the diffusion of boron, silicon, and some trivalent (3+) and quadrivalent (4+) elements and compounds across membranes [\u003cspan additionalcitationids=\"CR18 CR19 CR20\" citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e].\u003c/p\u003e\u003cp\u003eAbiotic stress has become a great challenge for plant growth and distribution due to climate change and environmental degradation, threatening food security and human survival and reproduction [\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. To date, many studies have evaluated the regulating roles of AQPs involved in abiotic stress responses through their normal roles as water channel proteins and in the transport of other small solute molecules [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. Among them, drought and high salinity/alkalinity are two major threatening factors that have a serious effect on plants living in special habitats, such as desert areas and coastal saline regions [\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e, \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e]. AQPs facilitate efficient transport of water molecules across membranes in almost all higher organisms in nature and, thus, they have been the focus of research decoding environmental challenges in plant genetics and crop improvement [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e, \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e]. For example, transgenic Arabidopsis and tobacco plants overexpressing \u003cem\u003eAtPIP1;4\u003c/em\u003e or \u003cem\u003eAtPIP2;5\u003c/em\u003e showed enhanced tolerance to salt, drought, and cold stress [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e]. \u003cem\u003eGmPIP1;6\u003c/em\u003e overexpression in soybean conferred enhanced salt tolerance and increased yield, mainly due to increased seed size [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e]. \u003cem\u003eMusaPIP1;2\u003c/em\u003e and \u003cem\u003eMusaPIP2;6\u003c/em\u003e overexpression in banana resulted in better abiotic stress survival characteristics compared to non-transformed control plants [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e, \u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e]. Similar results have also been presented in barley [\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e], tomato [\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e], apple [\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e], citrus [\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e], wheat [\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e, \u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e], and many special habitat plants. However, some overexpression assays for individual \u003cem\u003eAQP\u003c/em\u003es have led to decreased tolerance to abiotic stress. For example, ectopic overexpression of \u003cem\u003eGlycine soja GsTIP2;1\u003c/em\u003e [\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e] and \u003cem\u003eGsPIP2;1\u003c/em\u003e [\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e] in \u003cem\u003eArabidopsis thaliana\u003c/em\u003e resulted in sensitivities to salt and dehydration stress. In our previous study, overexpression of \u003cem\u003eCanavalia rosea CrPIP1;5\u003c/em\u003e in Arabidopsis caused weak sensitivity to drought treatment [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e], while \u003cem\u003eCrPIP2;3\u003c/em\u003e overexpression increased resistance to drought in transgenic Arabidopsis [\u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e]. CrPIP1;5 and CrPIP2;3 proteins interact, likely forming a heterotetramer water channel and performing a water transport function across the cell membrane [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e, \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e]. Both the tolerance and sensitivity to drought or salt stress hinge on the water transport efficiency mediated by specific plant AQPs. The different sites or pathways of water transport (from outside into plant or from the plant to its surroundings) might present the exact opposite effects in transgenic plant phenotypes, which further depends on the localization or tissue-specific distribution of the ectopic accumulation of transgenic AQPs and the protein complex interacting with the AQPs, acting as functional modules.\u003c/p\u003e\u003cp\u003eIn recent years, molecular biology research on special habitat plants has focused on their mechanisms or pathways for ecological flexibility and adaptability exploration. A meaningful research focus is the halophyte \u003cem\u003eAQP\u003c/em\u003e families due to their effects on a range of physiological processes, including water-deficit and high-osmotic stress [\u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e42\u003c/span\u003e]. \u003cem\u003eEutrema salsugineum\u003c/em\u003e, an extremophile model species for studying salt resistance, is closely related to \u003cem\u003eA. thaliana\u003c/em\u003e. There are 35 \u003cem\u003eEsAQP\u003c/em\u003es in the \u003cem\u003eE. salsugineum\u003c/em\u003e genome, and their expression is responsive to salt, drought, and cold stress [\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e]. \u003cem\u003eEsPIP1;2\u003c/em\u003e and \u003cem\u003eEsPIP1;4\u003c/em\u003e overexpression in Arabidopsis resulted in a salt stress-sensitive phenotype [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Olive trees (\u003cem\u003eOlea europaea\u003c/em\u003e L.) [\u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e44\u003c/span\u003e] and jujube (\u003cem\u003eZiziphus jujuba\u003c/em\u003e Mill.) [\u003cspan citationid=\"CR45\" class=\"CitationRef\"\u003e45\u003c/span\u003e] are both drought-resistant plants, and their \u003cem\u003eAQP\u003c/em\u003e families have been systematically characterized based on the structure and functional or evolution diversity of different subfamilies. Pearl millet (\u003cem\u003ePennisetum glaucum\u003c/em\u003e) is a cereal grown for food, grain, and straw in arid and semi-arid regions. Based on its genome sequencing information, the \u003cem\u003ePgAQP\u003c/em\u003e family in pearl millet has been characterized using a genomic approach, and functional expression analyses have been performed [\u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e46\u003c/span\u003e]. \u003cem\u003eAcacia auriculiformis\u003c/em\u003e is a commercially important forest tree with strong drought tolerance and 21 \u003cem\u003eAaAQP\u003c/em\u003e genes, and functional prediction has been performed based on bioinformatics analysis and gene expression profiles. \u003cem\u003eAaPIP1-2\u003c/em\u003e overexpression in Arabidopsis promotes tolerance to drought stress, further providing experimental evidence for the regulation of abiotic stress responses by \u003cem\u003eAQP\u003c/em\u003es [\u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e47\u003c/span\u003e]. Goji (\u003cem\u003eLycium barbarum\u003c/em\u003e) is an important food and medical plant that adapts well to strong light, arid, and saline environments. A total of 38 \u003cem\u003eLbAQP\u003c/em\u003es have been identified and characterized through systemic bioinformatics and expression analyses, and their possible roles in abiotic stress responses have been further studied through transcript analysis [\u003cspan citationid=\"CR48\" class=\"CitationRef\"\u003e48\u003c/span\u003e]. Mangrove plants \u003cem\u003eKandelia obovata\u003c/em\u003e and \u003cem\u003eAvicennia marina\u003c/em\u003e both present excellent adaptability to extreme environments, such as high salinity and tidal inundation, and the \u003cem\u003eKoAQP\u003c/em\u003e (34 members) and \u003cem\u003eAmAQP\u003c/em\u003e families (46 members) have been systematically identified, further demonstrating their possible environmental adaptation [\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e49\u003c/span\u003e, \u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e50\u003c/span\u003e]. Woody plant \u003cem\u003ePopulus euphratica\u003c/em\u003e possesses excellent drought and salt tolerance, and it is often used as a model forest tree in arid regions. The \u003cem\u003ePeuAQP\u003c/em\u003e gene family is involved in the tolerance and responses to different abiotic stressors [\u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e51\u003c/span\u003e]. Based on released plant genome sequencing information, gene family identification is a simple method for exploring molecular mechanisms and gene functions in plant molecular biology.\u003c/p\u003e\u003cp\u003e\u003cem\u003eTetragonia tetragonioides\u003c/em\u003e, also called New Zealand spinach and French spinach, is a halophyte adapted to environments with high salinity and temperature extremes, especially arid and semi-arid areas. The native habitat and planting areas of \u003cem\u003eT. tetragonioides\u003c/em\u003e often have high saline/alkaline paddy soil and are threatened by unpredictable seawater flow, thereby causing destruction of the ecological environment and economic losses for farmers. Determining the possible mechanisms by which \u003cem\u003eT. tetragonioides\u003c/em\u003e responds to salinity/alkalinity and drought/high osmotic stress is of critical importance. As an atypical succulent plant, the abundant water storage in the aboveground parts of \u003cem\u003eT. tetragonioides\u003c/em\u003e is the key factor for its development, enabling survival and adaptation in its natural habitat. Water absorption, storage, and redistribution in plants \u003cem\u003ein vivo\u003c/em\u003e depend critically on the specific functions of multiple AQPs. Through the functional analyses of this extremophile\u0026rsquo;s \u003cem\u003eTtAQP\u003c/em\u003es, our research paves the way for a better understanding of the roles of \u003cem\u003eTtAQP\u003c/em\u003es, the stress tolerance mechanism, and the ecological adaptability of this halophyte. In this study, we searched the \u003cem\u003eT. tetragonioides\u003c/em\u003e genome database for all \u003cem\u003eTtAQP\u003c/em\u003e members and conducted \u003cem\u003eTtAQP\u003c/em\u003e family-based association studies. Their expression profiles were systematically evaluated to elucidate the association of \u003cem\u003eTtAQP\u003c/em\u003es with the molecular mechanism of ecological adaptation. As a result, 58 \u003cem\u003eTtAQP\u003c/em\u003e genes were identified and further phylogenetic analysis, protein structure prediction, \u003cem\u003ecis\u003c/em\u003e-acting element (CE) analysis for promoters, and yeast heterologous function validation were explored. The results of our study also offer a foundation and genetic resources for further plant breeding of species with high stress tolerance, especially those that can survive and breed in areas that are arid and have seawater flow.\u003c/p\u003e"},{"header":"Materials and methods","content":"\u003cp\u003e\u003cb\u003ePlant materials, growth conditions, and stress treatments\u003c/b\u003e\u003c/p\u003e\u003cp\u003e\u003cem\u003eTetragonia tetragonoides\u003c/em\u003e seeds were germinated, and the seedlings or adult plants were cultivated as previously described [\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e52\u003c/span\u003e]. The four stress treatments for \u003cem\u003eT. tetragonoides\u003c/em\u003e seedlings were set as follows: heat (45℃), high osmotic stress (mimicking drought stress, 300 mM mannitol), high salinity (600 mM NaCl), and high alkalinity (150 mM NaHCO\u003csub\u003e3\u003c/sub\u003e, pH 8.2). Briefly, the \u003cem\u003eT. tetragonoides\u003c/em\u003e seedlings were moved to the 45℃ illumination incubator from a greenhouse (22\u0026ndash;26℃) for different periods to simulate the heat treatment. The seedlings were removed from their pots, which contained vermiculite, and carefully cleaned. They were then transferred, with their roots submerged into solutions to perform the other three stress treatments. Half-strength Murashige and Skoog (1/2 MS) liquid medium was the basal solution for preparing the treatment solutions. Seedlings treated with standard 1/2 MS liquid medium were set as controls.\u003c/p\u003e\u003cp\u003e\u003cb\u003eDatabase search for\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es in\u003c/b\u003e \u003cb\u003eT. tetragonoides\u003c/b\u003e \u003cb\u003eand chromosomal localization of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe \u003cem\u003eT. tetragonoides\u003c/em\u003e genome was sequenced and submitted to the NCBI database (NCBI accession number: JBBMRK000000000, unreleased data). All of the predicted proteins were annotated with InterProscan (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.ebi.ac.uk/interpro/search/sequence/\u003c/span\u003e\u003cspan address=\"https://www.ebi.ac.uk/interpro/search/sequence/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). The conserved MIP domain (PF00230 or IPR000425) was then searched as a model, and the protein sequences containing this domain were screened using HMM3.0 software. The domains were also confirmed using the NCBI CDD program (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.ncbi.nlm.nih.gov/cdd/\u003c/span\u003e\u003cspan address=\"https://www.ncbi.nlm.nih.gov/cdd/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). \u003cem\u003eT. tetragonoides\u003c/em\u003e proteins with a MIP domain and their corresponding genes were identified as belonging to the \u003cem\u003eTtAQP\u003c/em\u003e family. The obtained \u003cem\u003eTtAQP\u003c/em\u003e nucleotide and encoded protein sequences from \u003cem\u003eT. tetragonoides\u003c/em\u003e are listed in Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003cb\u003eGene structure analysis of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es and homologous analysis of TtAQP family members\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe gene structures (exon\u0026ndash;intron structures) of \u003cem\u003eT. tetragonoides AQP\u003c/em\u003e family members were analyzed using the online tool MEME (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://meme-suite.org/tools/meme\u003c/span\u003e\u003cspan address=\"http://meme-suite.org/tools/meme\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e), and the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome sequencing data and conserved TtAQP motifs were analyzed based on a predicted value of 10. TBtools [\u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e53\u003c/span\u003e] was used to visualize and map the obtained gene structure and the distribution of introns and exons, and the conserved motif information was visualized and mapped.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhylogenetic and conserved motif analysis of the TtAQP proteins\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe AQP protein sequences of \u003cem\u003eT. tetragonoides\u003c/em\u003e were compared with members of bay bean (\u003cem\u003eC. rosea\u003c/em\u003e, CrAQPs) [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e], rice (\u003cem\u003eOryza sativa\u003c/em\u003e, OsAQPs) [\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e], and \u003cem\u003eA. thaliana\u003c/em\u003e (AtAQPs) [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e] using the ClustalW algorithm. A phylogenetic tree was constructed with MEGA X software using the neighboring-joining (NJ) method. Based on the results of phylogenetic analysis, TtAQPs were categorized into five classes: PIPs, TIPs, NIPs, SIPs, and XIPs. The nomenclature of these TtAQPs was determined accordingly. The CrAQP, OsAQP, and AtAQP sequences of these three species are listed in Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003eFunctional amino acid predictions, such as NPA motifs, ar/R filters (H2, H5, LE1, and LE2), and Froger\u0026rsquo;s positions (P1\u0026ndash;P5), were analyzed based on the alignment with AQPs with a known function [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e, \u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e]. The 3D structures of TtAQPs were generated with the Phyre\u003csup\u003e2\u003c/sup\u003e server (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=contact\u003c/span\u003e\u003cspan address=\"https://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=contact\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). The subcellular localization patterns of all TtAQPs were predicted with WoLF_PSORT (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://wolfpsort.hgc.jp/\u003c/span\u003e\u003cspan address=\"https://wolfpsort.hgc.jp/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e) and Plant-mPLoc (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://www.csbio.sjtu.edu.cn/bioinf/plant-multi/\u003c/span\u003e\u003cspan address=\"http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e) programs.\u003c/p\u003e\u003cp\u003e\u003cb\u003eDuplication event investigation and synteny analysis of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e \u003cb\u003egenes\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTo further analyze the \u003cem\u003eT. tetragonoides\u003c/em\u003e AQP family genes and the different duplication patterns of TtAQP members, the duplication events of the AQP genes were analyzed using MCScanX software, and tandem duplications were checked manually according to their gene loci. In addition to the single (SL) gene pattern, the five normal gene duplication patterns, namely whole genome duplication (WGD), tandem duplication (TD, two adjacent duplicate genes), proximal duplication (PD, repeated genes within a 10-gene interval), transposed duplication (repetitive genes composed of ancestors and new loci), and dispersed duplication (DD, repetitive genes that are neither adjacent nor collinear), were determined. Chromosome distribution maps of the covariance of \u003cem\u003eTtAQP\u003c/em\u003es were plotted using Advanced Circos in TBtools software. The number of synonymous substitutions per synonymous site (Ka), the number of non-synonymous substitutions per nonsynonymous site (Ks), and the P-value from Fisher\u0026rsquo;s exact test of neutrality were calculated using the Nei-Gojobori model with 1000-bootstrap replicates [\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e55\u003c/span\u003e]. A Ka/Ks ratio\u0026thinsp;\u0026lt;\u0026thinsp;1 indicates purifying selection, a Ka/Ks ratio\u0026thinsp;=\u0026thinsp;1 indicates neutral selection, and a Ka/Ks ratio\u0026thinsp;\u0026gt;\u0026thinsp;1 indicates positive selection.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePromoter sequence profiles of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe nucleotide sequences 2000 bp upstream from the start codon (ATG) were extracted as the promoter sequence, and CEs\u0026rsquo; prediction of the promoter was performed using PlantCARE (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://bioinformatics.psb.ugent.be/webtools/plantcare/html/\u003c/span\u003e\u003cspan address=\"https://bioinformatics.psb.ugent.be/webtools/plantcare/html/\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). The heat-stress elements (HSEs), including 12 putative sequences that have been confirmed in Arabidopsis, rice, maize, and soybean, were also identified manually according to a previous report [\u003cspan citationid=\"CR56\" class=\"CitationRef\"\u003e56\u003c/span\u003e]. Then, the CE information was further analyzed and shown as a map with TBtools software. The CE sequence information is summarized in Table S2.\u003c/p\u003e\u003cp\u003e\u003cb\u003eExpression analysis of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es\u003c/b\u003e\u003c/p\u003e\u003cp\u003eExpression data for \u003cem\u003eTtAQP\u003c/em\u003es in \u003cem\u003eT. tetragonoides\u003c/em\u003e seedlings treated with heat and other stress treatments, as described previously, were collected from an RNA sequencing (RNA-seq) dataset (unpublished), and the fragments per kilobase of transcript per million mapped reads (FPKM) values were processed according to previous publications [\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e52\u003c/span\u003e]. Briefly, the expression levels [log\u003csub\u003e2\u003c/sub\u003e (FPKM\u0026thinsp;+\u0026thinsp;1)] of \u003cem\u003eTtAQP\u003c/em\u003es were visualized as heatmaps using TBtools software. The FPKM values for all samples are listed in Table S3.\u003c/p\u003e\u003cp\u003eQuantitative reverse transcription polymerase chain reaction (qRT-PCR) was also performed to detect the transcript abundance of several \u003cem\u003eTtAQP\u003c/em\u003e transcripts according to a previous report [\u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e52\u003c/span\u003e]. Briefly, 14 candidate \u003cem\u003eTtAQP\u003c/em\u003e genes, namely six \u003cem\u003eTtPIP\u003c/em\u003es (\u003cem\u003eTtPIP1;1\u003c/em\u003e, \u003cem\u003eTtPIP1;2\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;2\u003c/em\u003e, \u003cem\u003eTtPIP2;7\u003c/em\u003e, and \u003cem\u003eTtPIP2;8\u003c/em\u003e), six \u003cem\u003eTtTIP\u003c/em\u003es (\u003cem\u003eTtTIP1;1\u003c/em\u003e, \u003cem\u003eTtTIP1;2\u003c/em\u003e, \u003cem\u003eTtTIP1;3\u003c/em\u003e, \u003cem\u003eTtTIP1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e, and \u003cem\u003eTtTIP2;2\u003c/em\u003e), and two \u003cem\u003eTtSIP\u003c/em\u003es (\u003cem\u003eTtSIP1;1\u003c/em\u003e and \u003cem\u003eTtSIP1;2\u003c/em\u003e), were initially selected based on their RNA-seq data. The expression data for these \u003cem\u003eTtAQP\u003c/em\u003es obtained via qRT-PCR were normalized to the expression of the reference gene \u003cem\u003eTtACT\u003c/em\u003e (NCBI accession No.: MH33308). The primers used for qRT-PCR (TtACTRTF/TtACTRTR as the reference gene and other \u003cem\u003eTtAQP\u003c/em\u003e-specific primer pairs) are listed in Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003cb\u003eIn vivo\u003c/b\u003e \u003cb\u003estress tolerance assay for\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e \u003cb\u003eoverexpression in yeast\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe eight candidate \u003cem\u003eTtAQP\u003c/em\u003es (\u003cem\u003eTtTIP1;1\u003c/em\u003e, \u003cem\u003eTtTIP1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP1;1\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;7\u003c/em\u003e, \u003cem\u003eTtNIP1;2\u003c/em\u003e, and \u003cem\u003eTtSIP1;2\u003c/em\u003e) were PCR cloned using cDNA from \u003cem\u003eT. tetragonoides\u003c/em\u003e as a template. Briefly, the open reading frames of candidate \u003cem\u003eTtAQP\u003c/em\u003es were amplified with gene-specific primer pairs (Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e). The PCR fragments were purified, inserted into the \u003cem\u003eBam\u003c/em\u003eH I and \u003cem\u003eEco\u003c/em\u003eR I sites of yeast expression vector pYES2, yielding recombinant plasmids of \u003cem\u003eTtAQP\u003c/em\u003es-pYES2, and sequenced. Different yeast strains were used in this study, including wild-type (WT) \u003cem\u003eSaccharomyces cerevisiae\u003c/em\u003e (BY47471; MATa; \u003cem\u003ehis3Δ1; leu2Δ0; met15Δ0; ura3Δ0\u003c/em\u003e; accession number: Y00000), two H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-sensitive mutant strains \u003cem\u003eskn7Δ\u003c/em\u003e (BY4741; MATa; \u003cem\u003ehis3Δ1\u003c/em\u003e; \u003cem\u003eleu2Δ0\u003c/em\u003e; \u003cem\u003emet15Δ0\u003c/em\u003e; \u003cem\u003eura3Δ0\u003c/em\u003e; YHR206w::kanMX4; accession number: Y02900) and \u003cem\u003eyap1Δ\u003c/em\u003e (BY4741; MATa; \u003cem\u003ehis3Δ1\u003c/em\u003e; \u003cem\u003eleu2Δ0\u003c/em\u003e; \u003cem\u003emet15Δ0\u003c/em\u003e; \u003cem\u003eura3Δ0\u003c/em\u003e; YML007w::kanMX4; accession number: Y00569), which were obtained from Euroscarf (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://www.euroscarf.de/index.php?name=News\u003c/span\u003e\u003cspan address=\"http://www.euroscarf.de/index.php?name=News\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e). The standard polyethylene glycol\u0026ndash;lithium acetate-based transformation procedure was used for yeast plasmid transformation with amino acid defect screening. The yeast spot assays for NaCl, NaHCO\u003csub\u003e3\u003c/sub\u003e, sorbitol, and oxidative stress tolerance (H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) were performed as previously described [\u003cspan citationid=\"CR57\" class=\"CitationRef\"\u003e57\u003c/span\u003e]. For the heat tolerance tests of yeast strains, yeast cultures with different specific OD600 values were placed on a thermostat (50 or 52\u0026deg;C) for different time treatments. The yeast cultures and series of 10-fold diluted yeast liquids were spotted on solid yeast synthetic defect medium plus galactose (SDG) medium plates. The plates were incubated at 30\u0026deg;C for 2\u0026ndash;5 days and photographed.\u003c/p\u003e\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003eStatistical analysis\u003c/h2\u003e\u003cp\u003eAll experiments in this study were repeated independently three times, and the results are shown as the mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard deviation (SD) (n\u0026thinsp;\u0026ge;\u0026thinsp;3). Pairwise differences between means were analyzed using a Student\u0026rsquo;s \u003cem\u003et\u003c/em\u003e-test in Microsoft Excel 2021.\u003c/p\u003e\u003c/div\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cb\u003eIdentification of\u003c/b\u003e \u003cb\u003eT. tetragonoides AQP\u003c/b\u003e \u003cb\u003efamily members\u003c/b\u003e\u003c/p\u003e\u003cp\u003eIn total, 58 \u003cem\u003eTtAQP\u003c/em\u003e genes were identified in the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome according to our previous genome annotation file (date unpublished) [\u003cspan citationid=\"CR57\" class=\"CitationRef\"\u003e57\u003c/span\u003e]. These predicted TtAQP proteins all contained the conserved MIP domain. The full-length transcriptome was generated for five \u003cem\u003eT. tetragonoides\u003c/em\u003e tissues, namely roots, stem, leaves, flowers, and young seeds, supporting their expression and characterizing them as real genes. Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e lists the characteristics of the 58 TtAQPs. All TtAQP members were named according to the classification of AQPs in model plant Arabidopsis.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eNomenclature and subcellular localization prediction of 58 TtAQPs.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"10\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c9\" colnum=\"9\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c10\" colnum=\"10\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eGene name\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eLocus\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eProtein Length (aa)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eMw (kD)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003epI\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eGRAVY\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c7\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eTMHs and Topologies\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c8\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eNo. of Phosphor Sites\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"2\" nameend=\"c10\" namest=\"c9\"\u003e\u003cp\u003ePrediction for Subcellular Localization\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c9\"\u003e\u003cp\u003eWoLF_PSORT\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c10\"\u003e\u003cp\u003ePlant-mPLoc\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0018570\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.39\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.16\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.453\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:8 Tyr:4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt16G0004630\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.37\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.17\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.469\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:9 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0019610\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e286\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.57\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.377\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:9 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0022590\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.44\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.389\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:7 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, E.R.: 3, chlo: 1, vacu: 1, pero: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt01G0010040\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.59\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.28\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.464\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer: 14 Thr: 7 Tyr: 2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 12, vacu: 1, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt11G0009920\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e288\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.70\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.28\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.464\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:12 Thr:8 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 12, vacu: 1, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt01G0015190\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e289\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.76\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.29\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.497\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:6 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, E.R.: 2, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt11G0014700\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e290\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.80\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.81\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.456\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:8 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 12, E.R.: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0003670\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.79\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.71\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.461\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:7 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0020820\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.79\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.71\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.461\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:7 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;7\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0020280\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e291\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e32.09\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.84\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.175\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:15 Thr:10 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, vacu: 2, cyto: 1, extr: 1, E.R.: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0000270\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e283\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.00\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.473\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:6 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 12, vacu: 2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt01G0010050\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e293\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.42\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.95\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.446\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:14 Thr:10 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt11G0009910\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e28.30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.37\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.240\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:11 Thr:6 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 13, chlo: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0003660\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e283\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e29.99\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.28\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.482\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:6 Tyr:5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt01G0009310\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.28\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.81\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.745\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:7 Thr:5 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 2, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt11G0010640\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.27\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.81\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.731\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:7 Thr:4 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 2, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0000820\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e251\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.76\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.62\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.861\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:2 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 13, plas: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0000850\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e252\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.88\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.89\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.836\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:3 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt09G0011690\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e213\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e22.03\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.99\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.844\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e5/out to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:4 Thr:3 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, vacu: 5, extr: 1, E.R.: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt15G0007390\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e252\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.19\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.784\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:4 Thr:3 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, vacu: 6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0018250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.22\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.875\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:4 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 8, plas: 6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0002320\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.22\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.00\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.823\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:5 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 9, plas: 5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0023790\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.21\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.42\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.969\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:12 Thr:3 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 12, plas: 1, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0013740\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e24.94\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.09\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e1.003\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:3 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 13, plas: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0023800\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.25\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e4.86\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.931\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:2 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0013750\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.17\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e5.39\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.965\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:1 Tyr:4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 12, plas: 1, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP3;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt05G0011610\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e257\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e27.05\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.07\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.629\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:4 Thr:6 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, vacu: 4, chlo: 1, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP3;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt12G0014560\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e257\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e27.05\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.07\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.629\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:4 Thr:6 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, vacu: 4, chlo: 1, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP4;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt09G0007910\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e231\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e24.19\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.63\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.819\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:3 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 11, plas: 2, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP4;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt15G0011210\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e251\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.19\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.03\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.785\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:5 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 7, vacu: 6, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP5;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0009470\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.45\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.535\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:15 Thr:8 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eE.R.: 5, plas: 3.5, cyto_plas: 2.5, chlo: 2, mito: 1, vacu: 1, pero: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane/Vacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP5;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0013390\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e206\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e21.52\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.578\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e2/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:5 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eE.R.: 3, pero: 3, chlo: 2, cyto: 2, plas: 2, vacu: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt08G0012500\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e294\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.21\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.08\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.380\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:16 Thr:9 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 1, E.R.: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt10G0005880\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e294\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.36\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.377\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:17 Thr:9 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 2, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0021270\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.01\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.571\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:3 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0021280\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.01\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.571\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:3 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0021260\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.01\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.571\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:13 Thr:3 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, E.R.: 4, vacu: 2, extr: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP3;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt11G0017300\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e288\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.52\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.19\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.459\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:18 Thr:8 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 2, E.R.: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP4;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0021290\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e157\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e16.43\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.73\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.687\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:6 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 11, plas: 1, extr: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP4;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt16G0007250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e30.34\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.66\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.469\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:16 Thr:11 Tyr:3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 9, vacu: 2, extr: 1, E.R.: 1, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP5;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt03G0015410\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e311\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e32.38\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.483\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:16 Thr:10 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 7, vacu: 3, E.R.: 3, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP5;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt16G0010870\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e308\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e32.08\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.460\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:15 Thr:10 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, E.R.: 3, vacu: 2, golg: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP6;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0016040\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e307\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.69\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.07\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.516\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:16 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, cyto: 1, extr: 1, vacu: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP6;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0004320\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e304\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e31.33\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.07\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.532\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:15 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 11, vacu: 2, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0020740\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.22\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.90\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.712\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:11 Thr:9 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 7, plas: 4, golg: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt04G0020840\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.22\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.90\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.712\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:11 Thr:9 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 7, plas: 4, golg: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0021490\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.30\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.05\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.720\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:10 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 7, plas: 4, golg: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0018640\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e325\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e34.76\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.45\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.413\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:17 Thr:4 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, golg: 4, vacu: 2, E.R.: 2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt06G0018660\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e325\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e34.83\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.78\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.388\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:17 Thr:4 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, golg: 3, E.R.: 2, vacu: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt10G0007570\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e229\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e23.88\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.89\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.835\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:3 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane/Vacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0018950\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e229\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e23.89\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e6.95\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.855\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:8 Thr:3 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane/Vacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt09G0010400\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.50\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.63\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.684\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:11 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 9, vacu: 4, cyto: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt15G0008730\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.49\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.57\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.680\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:11 Tyr:2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 8, vacu: 5, cyto: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt02G0020900\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e233\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.49\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.48\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.652\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:7 Thr:5 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 6, plas: 5, E.R.: 3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt14G0020550\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e233\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e25.41\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e9.41\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.682\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:10 Thr:5 Tyr:0\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 6, plas: 5, E.R.: 2, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtXIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt07G0019520\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e246\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e26.13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e8.52\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.975\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:9 Thr:9 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003evacu: 13, extr: 1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane/Vacuole\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtXIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003eTt13G0001000\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e321\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c4\"\u003e\u003cp\u003e34.48\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c5\"\u003e\u003cp\u003e7.62\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c6\"\u003e\u003cp\u003e0.764\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e6/in to in\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSer:14 Thr:10 Tyr:1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eplas: 6, vacu: 6, E.R.: 2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eCell membrane\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eThere were 15 TtPIPs, 18 TtTIPs, 19 TtNIPs, 4 TtSIPs, and 2 TtXIPs, and the TtPIPs were further divided into 4 TtPIP1s and 11 TtPIP2s (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Based on the analysis of the physical and chemical properties of the TtAQPs, the protein lengths of the TtAQPs ranged from 157 (TtNIP4;1) to 325 aa (TtNIP8;1 and TtNIP8;2), with molecular weights ranging from 16.43 to 34.83 kDa. The isoelectric point values of TtAQPs were between 4.86 (TtTIP2;5) and 9.57 (TtSIP1;2), and most of them (35 of 58) were above 7, indicating that they are basic proteins. Additionally, the grand average of hydropathicity (GRAVY) was between 0.175 (TtPIP2;7) and 1.003 (TtTIP2;4), indicating that they are all hydrophobic proteins (GRAVY\u0026thinsp;\u0026gt;\u0026thinsp;0). The phosphorylation modification for AQPs is one of the key factors determining the subcellular distribution and trafficking of proteins, which further determines the biochemical functions of these membrane proteins [\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e58\u003c/span\u003e]. All TtAQPs had more than one phosphorylation amino acid site, including serine, threonine, and tyrosine. The subcellular localization prediction for TtAQPs was performed with different programs, including WoLF_PSORT and Plant-mPLoc (Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). As plasma membrane and endomembrane intrinsic proteins, the subcellular localization prediction for TtAQPs was roughly symmetrical with their nomenclature, especially the prediction values from the WoLF_PSORT program. The presence of transmembrane domains in each TtAQP member provides the necessary conditions for water molecular cross-membrane transportation (Figure \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e), which depends on the pore formed by a series of membrane helices linked by helix loops. The subcellular localization of specific TtAQPs might be determined more specifically by post-translational modifications or protein interactions [\u003cspan citationid=\"CR59\" class=\"CitationRef\"\u003e59\u003c/span\u003e]. The multiplicity of TtAQPs\u0026rsquo; localization and modifications further determines the diversity and complexity of their biochemical and biological functions.\u003c/p\u003e\u003cp\u003eThe features of TtAQPs were also predicted with the InterPro online program. As shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and Figure \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e, all TtAQPs were membrane-anchored proteins with 2\u0026ndash;6 trans-membrane α-helices, which form pores for water or other small molecules transported as heterotetramers [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. The MIP conserved domain was present in most TtAOP proteins (Figure S2). The N-terminal of the TtAOPs was usually a signal peptide, which might directly determine the subcellular localization of each TtAQP \u003cem\u003ein vivo\u003c/em\u003e.\u003c/p\u003e\u003cp\u003e\u003cb\u003eEvolutionary characterization of AQP proteins\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTo uncover their classification and evolutionary relationships, an unrooted phylogenetic tree from the deduced protein sequences of all TtAQPs was constructed (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e). Almost all TtAQP members were in pairs, except TtPIP2;11, TtNIP2;3, TtNIP3;1, and TtNIP7;3. The sequence similarities between two members in the same pair were extremely high, indicating that the genetic homology of these genes is very high. XIPs are uncharacterized AOPs [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. Here, the two TtXIPs were very similar to TtPIPs, further suggesting their possible similar evolutionary origins.\u003c/p\u003e\u003cp\u003eTo study the evolutionary relationship of AQP family proteins among plant species, an ML tree was created with AQP proteins from \u003cem\u003eA\u003c/em\u003e. \u003cem\u003ethaliana\u003c/em\u003e (dicotyledonous model plant), \u003cem\u003eO\u003c/em\u003e. \u003cem\u003esativa\u003c/em\u003e (monocotyledonous model plant), and \u003cem\u003eC\u003c/em\u003e. \u003cem\u003erosea\u003c/em\u003e (typical halophyte). Five distinct clades or subfamilies (PIP, TIP, NIP, SIP, and XIP) were clearly distinguished (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e), and PIPs, TIPs, and NIPs were relatively bigger, while the XIP clade was smallest. Due to \u003cem\u003eT. tetragonoides\u003c/em\u003e being a dicotyledonous plant, TtAQPs were more similar to AtAQPs and CrAQPs, and the total number of TtAQPs (58) was much greater than that of AtAQPs (35), OsAQPs (34), and CrAQPs (37). Their genome sizes did not show large differences (\u003cem\u003eT. tetragonoides\u003c/em\u003e: 400 Mb; Arabidopsis: 125 Mb; Rice: 385 Mb; \u003cem\u003eC\u003c/em\u003e. \u003cem\u003erosea\u003c/em\u003e: 535 Mb).\u003c/p\u003e\u003cp\u003eWe also compared the number of \u003cem\u003eAQP\u003c/em\u003e genes in \u003cem\u003eT. tetragonoides\u003c/em\u003e with that in other plants (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e), including two Brassicaceae species, nine Leguminosae species, five Gramineae species, and four other typical special habitat plants. The number of \u003cem\u003eAQP\u003c/em\u003e genes in all of these typical diploid species was below 50, and only the paleotetraploid genome of soybean (\u003cem\u003eG\u003c/em\u003e. \u003cem\u003emax\u003c/em\u003e) contained more \u003cem\u003eAQP\u003c/em\u003e members (72). The number of \u003cem\u003eTtAQP\u003c/em\u003es was relatively high, although this species is a typical diploid with a normal genome size (400 Mb).\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eThe numbers of \u003cem\u003eAQP\u003c/em\u003e genes in different plant species.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"8\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eFamily/Category\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eSpecies\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eTotal No.\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colspan=\"5\" nameend=\"c8\" namest=\"c4\"\u003e\u003cp\u003eThe No. of \u003cem\u003eAQP\u003c/em\u003es in sub-families\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003ePIPs\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eTIPs\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eNIPs\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eSIPs\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eXIPs\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eBrassicaceae\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eArabidopsis thaliana\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e35\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eEutrema salsugineum\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e35\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"3\" rowspan=\"4\"\u003e\u003cp\u003eLeguminosae\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eCanavalia rosea\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e37\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e1\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eCicer arietinum\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e40\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e16\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eGlycine max (paleotetraploid)\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e72\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e24\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e24\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e17\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eMedicago truncatula\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e45\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e14\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e17\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eGramineae\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eOryza sativa\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e34\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eSetaria italica\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e39\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\" morerows=\"4\" rowspan=\"5\"\u003e\u003cp\u003eSpecial habitat plants\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eKandelia obovata\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e34\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e13\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e1\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eAvicennia marina\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e46\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e15\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e7\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003eZiziphus jujuba\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e36\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e12\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e1\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cem\u003ePopulus euphratica\u003c/em\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e22\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e0\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e\u003cb\u003eTetragonia tetragonoides\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e\u003cb\u003e58\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e\u003cb\u003e15\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e\u003cb\u003e18\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\u003cb\u003e19\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e\u003cb\u003e4\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003e\u003cb\u003e2\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eConserved amino acid residues (Asn-Pro-Ala, NPA) motifs, aromatic/arginine (ar/R) filters and Froger\u0026rsquo;s positions (FPs) and trans-membrane (TM) domains of TtAQPs in \u003cem\u003eT. tetragonoides\u003c/em\u003e.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"15\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c7\" colnum=\"7\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c8\" colnum=\"8\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c9\" colnum=\"9\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c10\" colnum=\"10\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c11\" colnum=\"11\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c12\" colnum=\"12\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c13\" colnum=\"13\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c14\" colnum=\"14\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c15\" colnum=\"15\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eProtein\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eNo. of aa\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eTM\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eNPA (LB)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eNPA (LE)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eNPA space\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"4\" nameend=\"c10\" namest=\"c7\"\u003e\u003cp\u003eAr/Rfilters\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colspan=\"5\" nameend=\"c15\" namest=\"c11\"\u003e\u003cp\u003eFroger\u0026rsquo;s residues\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH2\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH5\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c9\"\u003e\u003cp\u003eLE1\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c10\"\u003e\u003cp\u003eLE2\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c11\"\u003e\u003cp\u003eP1\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c12\"\u003e\u003cp\u003eP2\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c13\"\u003e\u003cp\u003eP3\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c14\"\u003e\u003cp\u003eP4\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c15\"\u003e\u003cp\u003eP5\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e286\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP1;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e288\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eMQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e289\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e290\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e287\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;7\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e291\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQM\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e283\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eM\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e293\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eLF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;10\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtPIP2;11\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e283\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eFVA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e118\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQ\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e251\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eAS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e252\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eAS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e213\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP1;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e252\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP2;6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e250\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP3;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e257\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP3;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e257\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eVR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP4;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e231\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e95\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP4;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e251\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e111\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eH\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP5;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e255\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtTIP5;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e206\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eVM\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eFG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eVT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eCL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e294\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eSW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eMI\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e294\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e109\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eLI\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e109\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eSW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eCV\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e109\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eSW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eCV\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP2;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e109\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eLV\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP3;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e288\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eLG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eNS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eSR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eVT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWM\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP4;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e157\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e\\\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e\\\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eG\\\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eSV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eLF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eMI\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP4;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e285\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e109\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eSW\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eIV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eTS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eMI\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP5;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e311\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e\\A\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eYR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eTF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eLT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP5;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e308\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eAI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eST\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eVL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP6;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e307\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eMC\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP6;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e304\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eFS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eHI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eTG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eST\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWC\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eLS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eNI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eAR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eVT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWM\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eVS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eNR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eMY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eAT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWM\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP7;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e253\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eVS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eIA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eNR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eMY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eAT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eWM\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e325\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eIL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e325\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eIL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e229\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eAV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eVL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtNIP8;4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e229\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e110\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eAV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eVL\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e112\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003e\\V\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAP\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFM\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eIW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e248\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPT\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e112\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eWV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAP\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eFM\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eIW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP2;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e233\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eLW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtSIP2;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e233\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e108\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eAS\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eAG\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eRA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eYL\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eY\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eLW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtXIP1;1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e246\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e134\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eVI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eFA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eVN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eC\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eTtXIP1;2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e321\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c3\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003eNPV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003eNPA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003e134\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c7\"\u003e\u003cp\u003eFV\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c8\"\u003e\u003cp\u003eHI\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c9\"\u003e\u003cp\u003eTA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c10\"\u003e\u003cp\u003eR\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c11\"\u003e\u003cp\u003eQN\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c12\"\u003e\u003cp\u003eSC\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c13\"\u003e\u003cp\u003eA\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c14\"\u003e\u003cp\u003eF\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c15\"\u003e\u003cp\u003eW\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eChromosomal locations and\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e \u003cb\u003egene structures\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe chromosome maps localizing \u003cem\u003eTtAQP\u003c/em\u003es were constructed with the purpose of investigating the gene family evolutionary relationship. There are 16 pairs of chromosomes in the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome. \u003cem\u003eTtAQP\u003c/em\u003e genes were found on all chromosomes, but their distribution was uneven. Chromosomes 02, 05, 08, 12, and 14 each had only one \u003cem\u003eTtAQP\u003c/em\u003e gene. Chromosome 10 contained two \u003cem\u003eTtAQP\u003c/em\u003es, and chromosomes 09, 15, and 16 each had three \u003cem\u003eTtAQP\u003c/em\u003es. Chromosome 01 had four, and chromosomes 03, 04, and 11 had five. Chromosome 07 had six, and chromosomes 06 and 13 had the most, with seven (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe exon\u0026ndash;intron structures of genes are essential for elucidating the possible molecular evolution of plant gene family members. The divergence of gene structures mainly occurs in duplicate gene evolution and gene sibling paralogs, which can result in functional gain or loss through specific exonization/pseudoexonization and insertion/deletion [\u003cspan citationid=\"CR60\" class=\"CitationRef\"\u003e60\u003c/span\u003e]. Based on phylogenetic and gene structure analyses (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e), most conjugated \u003cem\u003eTtAQP\u003c/em\u003es presented similar exon\u0026ndash;intron patterns, despite the fact that the lengths of introns and untranslated regions (UTRs) were slightly different. Only \u003cem\u003eTtTIP1;5\u003c/em\u003e/\u003cem\u003eTtTIP1;6\u003c/em\u003e, \u003cem\u003eTtTIP5;1\u003c/em\u003e/\u003cem\u003eTtTIP5;2\u003c/em\u003e, \u003cem\u003eTtXIP1;1\u003c/em\u003e/\u003cem\u003eTtXIP1;2\u003c/em\u003e, \u003cem\u003eTtPIP2;9\u003c/em\u003e/\u003cem\u003eTtPIP2;10\u003c/em\u003e, and \u003cem\u003eTtNIP4;1\u003c/em\u003e/\u003cem\u003eTtNIP4;2\u003c/em\u003e showed different gene structures. This high degree of similarity in protein sequences and gene structures further indicates that homologous gene duplication resulted in \u003cem\u003eTtAQP\u003c/em\u003e gene family amplification.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eDuplication event investigation\u003c/b\u003e\u003c/p\u003e\u003cp\u003eGene duplication events for \u003cem\u003eTtAQPs\u003c/em\u003e were investigated (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e, Table S2). As a main driving force of genome evolution, the phenomenon of gene duplication is widespread among the genomes of all organisms. To analyze the evolutionary process among \u003cem\u003eTtAQP\u003c/em\u003e family genes, genome-wide duplication events among these members were identified and analyzed, and MCScan X software was used to draw Circos plots. This analysis showed that 46 genes formed genome-wide duplication pairs, that the 23 pairs of homologous genes were segmentally duplicated, and that the duplication pairs resulted in many homologues of \u003cem\u003eTtAQPs\u003c/em\u003e among chromosomes, increasing the likelihood of evolution (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e). These findings suggest that gene duplication events play an important role in \u003cem\u003eTtAQP\u003c/em\u003e expansion.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eWe also found that most \u003cem\u003eTtAQPs\u003c/em\u003e were generated by WGD (47 of 58 \u003cem\u003eTtAQPs\u003c/em\u003e), and only TtPIP2;5, TtTIP2;5, TtTIP2;6, and TtNIP2;1 were generated by TD, and the PD pattern was only observed for TtNIP7;2 and TtNIP8;2. TtPIP2;3, TtPIP2;4, TtTIP3;1, TtTIP3;2, and TtNIP3;1 showed the DD pattern.\u003c/p\u003e\u003cp\u003eThroughout evolution, evolutionary forces and natural pressures inevitably affect the duplicated genes, showing different patterns. To understand the evolutionary divergence between paralogous gene pairs, Ka/Ks analysis was carried out in the \u003cem\u003eTtAQP\u003c/em\u003e gene family. A Ka/Ks ratio of more than 1 (Ka/Ks\u0026thinsp;\u0026gt;\u0026thinsp;1) suggests positive selection pressure (non-purifying), and a ratio less than 1 (Ka/Ks\u0026thinsp;\u0026lt;\u0026thinsp;1) indicates negative (purifying) selection pressure. A ratio equal to 1 (Ka/Ks\u0026thinsp;=\u0026thinsp;1) indicates neutral selection. All paralogous genes showed a Ka/Ks ratio much less than 1 (Ka/Ks\u0026thinsp;\u0026lt;\u0026thinsp;1), suggesting negative or purifying selection on duplicated \u003cem\u003eTtAQP\u003c/em\u003e genes (Table S3).\u003c/p\u003e\u003cp\u003e\u003cb\u003eCis\u003c/b\u003e\u003cb\u003e-acting elements (CEs) of the\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e \u003cb\u003epromoter sequences\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe gene expression patterns are closely related to the biological functions, and transcriptional regulation is directly under the control of the gene promoters. Prediction of the primary structures of \u003cem\u003eTtAQP\u003c/em\u003e gene promoter regions (ATP upstream 2000 bp) can clarify the transcriptional regulatory functions of gene family members. The CEs located in the promoter regions of the 58 \u003cem\u003eTtAQP\u003c/em\u003e members were analyzed (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e). PlantCARE predicted that all promoter regions of TtAQPs presented various functions for CEs, including biotic and abiotic stress responses. The MYB- (including MBS) and MYC-recognition sites were found in almost all \u003cem\u003eTtAQP\u003c/em\u003e gene promoter regions, indicating the basic regulatory roles mediated by the transcription factors (TFs), MYBs and MYCs. Plant MYBs and MYCs are ubiquitous TFs, and many previous reports have shown that these TFs are involved in plant salt and drought responses [\u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e61\u003c/span\u003e]. Additionally, HSEs are typically recognized by heat shock TFs (HSFs), thereby regulating the expression of the target genes. In all \u003cem\u003eTtAQP\u003c/em\u003e promoter regions, there were many HSEs, indicating that the water imbalance caused by unpredictable heat treatment could be alleviated by the multiple water transport activities of TtAQPs, and this occurs because of the heat regulated expression patterns of \u003cem\u003eTtAQP\u003c/em\u003es mediated by the HSEs in their promoter regions. In addition, the hormone-response CEs, including ABREs (abscisic acid-responsive), TGACG- and CGTCA- motifs (jasmonic acid-responsive), TCA-element (salicylic acid-responsive), TATC-box (gibberellic acid-responsive), and AuxRR-core (auxin-responsive), also irregularly existed in the promoter regions of \u003cem\u003eTtAQP\u003c/em\u003es (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e; Table S4), indicating that \u003cem\u003eTtAQP\u003c/em\u003e expression was influenced by hormones and related development or stress responses. The diversity of multiple CEs in \u003cem\u003eTtAQP\u003c/em\u003e promoter regions further explained the possible roles for water transport \u003cem\u003ein vivo\u003c/em\u003e mediated by specific TtAQPs, which was affected by their specific expression patterns under the control of their own promoters. The highly precise regulatory mechanism for \u003cem\u003eTtAQP\u003c/em\u003e expression further reflects the complexity of functions of these water channel proteins, such as in mediating plant growth, development, and biotic/abiotic stress responses.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eGlobal expression profiles of\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es in different tissues and plants under stress\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTo reveal the global expression profiles of \u003cem\u003eTtAQP\u003c/em\u003e genes, RNA-seq data for five tissues/developmental stages with three biological repeats each were examined (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e; Table S5). Plant AQPs have been shown to be engaged in plant growth and development, although the fundamental roles of AQPs involve the transport of H\u003csub\u003e2\u003c/sub\u003eO, other small molecules, and some specific chemical elements [\u003cspan citationid=\"CR62\" class=\"CitationRef\"\u003e62\u003c/span\u003e]. In some plants, their \u003cem\u003eAQP\u003c/em\u003e subfamily members show similar expression patterns, indicating their synergistic roles in the transport of water or other molecules in some organs. For instance, the mRNA of \u003cem\u003ePIPs\u003c/em\u003e and \u003cem\u003eTIPs\u003c/em\u003e is abundant in all organs in many plant species [\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e]. Our result showed that the expression of \u003cem\u003eTtAQPs\u003c/em\u003e among different tissues, including roots, stems, leaves, flowers, and young seeds, was organ specific, and the \u003cem\u003eTtPIPs\u003c/em\u003e and \u003cem\u003eTtTIPs\u003c/em\u003e presented relatively higher expression levels in all organs (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e; Figure S3). Some \u003cem\u003eTtPIP\u003c/em\u003e and \u003cem\u003eTtTIP\u003c/em\u003e members showed remark-\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eably high expression levels in the stems and leaves of \u003cem\u003eT. tetragonoides\u003c/em\u003e plants, and the \u003cem\u003eTtTIP3;1\u003c/em\u003e/\u003cem\u003eTtTIP3;2\u003c/em\u003e gene pair showed a much higher expression level in flowers than in other organs. These specific expression patterns further demonstrate the important roles of \u003cem\u003eTtAQPs\u003c/em\u003e in the growth and development of \u003cem\u003eT. tetragonoides\u003c/em\u003e.\u003c/p\u003e\u003cp\u003eThe disturbed water balance of plants \u003cem\u003ein vivo\u003c/em\u003e is often caused by a series of abiotic stressors, which are the main limiting factors for plants\u0026rsquo; adaptation to environmental conditions, such as osmotic stress generated from high salinity/alkalinity, drought, and heat. To determine the possible functions of \u003cem\u003eTtAQPs\u003c/em\u003e in abiotic stress response, we examined the effects of heat, salt, alkaline, and high osmotic (mimicking drought) treatments on the expression of all \u003cem\u003eTtAQP\u003c/em\u003e genes (Fig.\u0026nbsp;\u003cspan refid=\"Fig7\" class=\"InternalRef\"\u003e7\u003c/span\u003e). In underground root tissues, heat stress decreased the expression of most \u003cem\u003eTtAQPs\u003c/em\u003e, while in aboveground parts (stems and leaves), heat stress increased the expression levels of some \u003cem\u003eTtAQP\u003c/em\u003e members, including \u003cem\u003eTtTIP1;1\u003c/em\u003e/\u003cem\u003e1;2\u003c/em\u003e, \u003cem\u003eTtTIP1;3\u003c/em\u003e/\u003cem\u003e1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e/\u003cem\u003e2;2\u003c/em\u003e, \u003cem\u003eTtPIP1;1\u003c/em\u003e/\u003cem\u003e1;2\u003c/em\u003e, \u003cem\u003eTtPIP1;3\u003c/em\u003e/\u003cem\u003e1;4\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e/\u003cem\u003e2;2\u003c/em\u003e, and \u003cem\u003eTtTIP2;7\u003c/em\u003e/\u003cem\u003e2;8\u003c/em\u003e gene pairs. Additionally, in root tissues, the salt, alkalinity, and high osmotic stress decreased the expression of most \u003cem\u003eTtAQPs\u003c/em\u003e, while in stems and leaves, some \u003cem\u003eTtAQPs\u003c/em\u003e, such as \u003cem\u003eTtTIP1;1\u003c/em\u003e, \u003cem\u003eTtTIP2;1, TtTIP2;1\u003c/em\u003e/\u003cem\u003e2;2\u003c/em\u003e, \u003cem\u003eTtPIP2;3\u003c/em\u003e, \u003cem\u003eTtPIP2;8\u003c/em\u003e, and \u003cem\u003eTtPIP2;9\u003c/em\u003e, were induced by these stress treatments, especially the short-term treatments (2 h). Unexpectedly, \u003cem\u003eTtSIP1;1\u003c/em\u003e showed upregulated expression in stems and leaves under long-term treatments (48 h), indicating that \u003cem\u003eTtSIP1;1\u003c/em\u003e is involved in these stress response pathways.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eWe further investigated the expression patterns of \u003cem\u003eTtAQPs\u003c/em\u003e under salt, alkalinity, drought (high osmotic), and heat stress by qRT-PCR with more detailed stress treatment times (Fig.\u0026nbsp;\u003cspan refid=\"Fig8\" class=\"InternalRef\"\u003e8\u003c/span\u003e). Most of the detected \u003cem\u003eTtAQP\u003c/em\u003es were upregulated under salt, alkalinity, and high osmotic stress at different time points or in different tissues, while only \u003cem\u003eTtPIP2;2\u003c/em\u003e, \u003cem\u003eTtTIP2;2\u003c/em\u003e, and \u003cem\u003eTtSIP1;2\u003c/em\u003e showed downregulated expression patterns under all challenges, further demonstrating the relationship between the mRNA abundance of \u003cem\u003eTtAQP\u003c/em\u003es and water transport dynamic changes in plants affected by stress treatment \u003cem\u003ein vivo\u003c/em\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eAbiotic stress tolerance of yeast heterologously expressing\u003c/b\u003e \u003cb\u003eTtAQP\u003c/b\u003e\u003cb\u003es\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe yeast heterogeneous expression system has become an important approach for characterizing AQP transmembrane transportation [\u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e63\u003c/span\u003e]. Here, we selected eight \u003cem\u003eTtAQP\u003c/em\u003e members and determined their possible roles in abiotic stress tolerance when overexpressed in different yeast strains. When expressed in WT yeast cells, \u003cem\u003eTtTIP1;2\u003c/em\u003e, \u003cem\u003eTtTIP1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e; \u003cem\u003eTtPIP1;1\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;7\u003c/em\u003e, \u003cem\u003eTtNIP1;2\u003c/em\u003e, and \u003cem\u003eTtSIP1;2\u003c/em\u003e enhanced the salt tolerance of WT under high NaCl stress (1.25 and 1.5 M NaCl). A higher salinity stress intensity (1.75 M NaCl) inhibited the growth of yeast expressing the pYES2 empty vector (EV) or eight \u003cem\u003eTtAQPs\u003c/em\u003e (Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003eA). Under alkali stress, NaHCO\u003csub\u003e3\u003c/sub\u003e treatment did not cause a distinguished growth status for yeast expressing EV or eight \u003cem\u003eTtAQPs\u003c/em\u003e (Figure S4A). The high osmotic stress caused by different sorbitol concentrations also affected the growth of WT expressing \u003cem\u003eTtAQPs\u003c/em\u003e to a certain extent, and \u003cem\u003eTtNIP1;2\u003c/em\u003e increased the sensitivity of WT to sorbitol. \u003cem\u003eTtPIP1;1\u003c/em\u003e and \u003cem\u003eTtSIP1;2\u003c/em\u003e enhanced the tolerance of WT to high osmotic stress compared to EV (Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003eB). For heat stress tolerance, \u003cem\u003eTtPIP1;1\u003c/em\u003e and \u003cem\u003eTtSIP1;2\u003c/em\u003e also improved the survival rate of WT yeast after 50℃ heat shock (20 min), while \u003cem\u003eTtTIP1;4\u003c/em\u003e, \u003cem\u003eTtTIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;1\u003c/em\u003e, \u003cem\u003eTtPIP2;7\u003c/em\u003e, and \u003cem\u003eTtNIP1;2\u003c/em\u003e enhanced the sensitivity of WT yeast to heat shock (Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003eC).\u003c/p\u003e\u003cp\u003eThe H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-sensitive yeast mutants \u003cem\u003eskn7Δ\u003c/em\u003e and \u003cem\u003eyap1Δ\u003c/em\u003e were also used to detect the transportation function of these eight \u003cem\u003eTtAQP\u003c/em\u003e members (Figs.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003eA and S4B). Both yeast \u003cem\u003eskn7\u003c/em\u003e and \u003cem\u003eyap1\u003c/em\u003e encode TFs involved in oxidative stress responses [\u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e64\u003c/span\u003e]. Our previous study confirmed that both \u003cem\u003eskn7Δ\u003c/em\u003e and \u003cem\u003eyap1Δ\u003c/em\u003e were sensitive to heat stress [\u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e65\u003c/span\u003e]. In this study, the selected eight \u003cem\u003eTtAQP\u003c/em\u003e members caused differing H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e sensitivities in both \u003cem\u003eskn7Δ\u003c/em\u003e (Fig.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003eA) and \u003cem\u003eyap1Δ\u003c/em\u003e (Figure S4B), indicating that these TtAQPs transport H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e but in different cellular membranes in yeast. Additionally, except \u003cem\u003eTtSIP1;2\u003c/em\u003e, the other seven \u003cem\u003eTtAQPs\u003c/em\u003e enhanced the sensitivity of \u003cem\u003eskn7Δ\u003c/em\u003e yeast to heat shock (Fig.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003eB). These results were similar to those of the heat sensitivity test in WT yeast (\u003cem\u003eTtPIP1;1\u003c/em\u003e caused similar tolerance as \u003cem\u003eTtSIP1;2\u003c/em\u003e), which may be ascribed to the \u003cem\u003eskn7\u003c/em\u003e mutation and related downstream signal pathways differing from those in WT.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003e\u003cem\u003eTetragonia tetragonoides\u003c/em\u003e is an atypical, succulent endo-reoretohalophyte with excellent salinity, alkalinity, drought, and heat tolerance. As a rare halophytic tropical leafy vegetable, it has emerged as a novel health food due to its salt bladders, which contain essential mineral elements and some secondary metabolites [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]. \u003cem\u003eT. tetragonoides\u003c/em\u003e grows wild on many tropical beaches, coastal regions, and islands, which present extreme adversities for normal plant growth, such as high salinity/alkalinity, seasonal drought, and high temperatures caused by direct sun-exposure. \u003cem\u003eT. tetragonoides\u003c/em\u003e has adapted to its low altitude and extreme environments through morphological, physiological, and molecular mechanisms. The typical features of \u003cem\u003eT. tetragonoides\u003c/em\u003e in salt bladders over the whole plant surface, including the leaf epidermis and surfaces of petioles and stems, are among the most distinctive salt-resistance features in higher plants, and about half of halophytes have characteristic salt bladders on their surfaces [\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e66\u003c/span\u003e]. Salt bladders can increase the salt tolerance of plants by sequestering excessive Na\u003csup\u003e+\u003c/sup\u003e away from metabolically active cells and storing water for osmotic adjustment, thereby providing plants with an opportunity to survive in saline environments [\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e66\u003c/span\u003e]. The epidermal cells containing salt bladders usually expand greatly in volume, even up to 1000 times larger than epidermal bladder cells (EBCs). The large vesicles/vacuoles in EBCs usually contain large amounts of salt ions or secondary metabolites, which can be transported quickly with water by AQPs to maintain appropriate osmotic pressure \u003cem\u003ein vivo\u003c/em\u003e. For example, \u003cem\u003eAtriplex canescens\u003c/em\u003e is a typical C4 secretohalophyte with salt bladders on the leaves. \u003cem\u003eAcAQPs\u003c/em\u003e in leaves showed increased expression patterns after NaCl stress, facilitating water balance in seedlings under low water potential conditions [\u003cspan citationid=\"CR67\" class=\"CitationRef\"\u003e67\u003c/span\u003e]. \u003cem\u003eMesembryanthemum crystallinum\u003c/em\u003e (also called ice plant, Aizoaceae) is a closely related species of \u003cem\u003eT. tetragonoides\u003c/em\u003e, and \u003cem\u003eMcAQPs\u003c/em\u003e are precisely regulated during salt stress to maintain water balance [\u003cspan citationid=\"CR68\" class=\"CitationRef\"\u003e68\u003c/span\u003e]. Since \u003cem\u003eT. tetragonoides\u003c/em\u003e can grow in harsh habitats and have strong water storage capacity to endure the high salinity/alkalinity and drought stress, we suspect that \u003cem\u003eTtAQPs\u003c/em\u003e play roles in the adaptation to such harsh environments, and identification of the \u003cem\u003eTtAQP\u003c/em\u003e family is necessary to elucidate the possible molecular adaptation mechanisms of this special habitat species.\u003c/p\u003e\u003cp\u003ePlant AQPs belong to the MIP family and mainly function as channel proteins known for their abilities to conduct water and/or small molecules across biological membranes [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. In recent years, studies have focused on the possible regulatory roles of plant membrane transporters and biotic or abiotic stress responses [\u003cspan citationid=\"CR69\" class=\"CitationRef\"\u003e69\u003c/span\u003e]. Considering the growth and ecological adaptability of plants, the water deficit caused by external environmental factors significantly affects the survival and development of plant species. The damage caused by water deficit can be mitigated through the channels\u0026rsquo; formation of various transporter proteins, such as AQPs, evolving specific physiological mechanisms adapted to adversity stress. In this study, we performed a genome-wide identification of the \u003cem\u003eTtAQP\u003c/em\u003e family using bioinformatics methods and analyzed the phylogenetic relationships among TtAQPs and AQP family members of other plant species. Additionally, the gene structure and chromosome distribution of \u003cem\u003eTtAQPs\u003c/em\u003e were determined through covariance analysis, and their possible duplication patterns were determined.\u003c/p\u003e\u003cp\u003eIn this study, 58 \u003cem\u003eTtAQP\u003c/em\u003e family members were identified from the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome. They included 15 \u003cem\u003eTtPIPs\u003c/em\u003e, 18 \u003cem\u003eTtTIPs\u003c/em\u003e, 19 \u003cem\u003eTtNIPs\u003c/em\u003e, 4 \u003cem\u003eTtSIPs\u003c/em\u003e, and 2 \u003cem\u003eTtXIPs\u003c/em\u003e. Overall, the number of \u003cem\u003eTtAQP\u003c/em\u003e gene family members in the diploid \u003cem\u003eT. tetragonoides\u003c/em\u003e genome was greater than that found in many other typical diploid plant species (Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e), which might be due to the duplication patterns of different genes for adaptive evolution and further functional differentiation of \u003cem\u003eTtAQP\u003c/em\u003e family in this species. The abundance of TtAQP isoforms in \u003cem\u003eT. tetragonoides\u003c/em\u003e is also attributed to the need for organelle- or tissue-development specificity, as well as the requirement for the precise control of water transport under specific environmental conditions. Similarly, although some plant AQPs have been named according to their possible subcellular localization, such as PIP for plasma membrane intrinsic protein, TIP for tonoplast intrinsic protein, and SIP for possible endoplasmic reticulum membrane localization, their virtual cellular localization might be dynamic and adjustable. Plant AQPs belong to membrane-anchored proteins with 2\u0026ndash;6 trans-membrane α-helices, which form pores for transporting water or other small molecules, such as heterotetramers [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e], and determine their subcellular dispersal. As shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, the phosphorylation modification sites for each TtAQP member were specific, and this post-translational level modification further determines the functional specificity for each TtAQP. The functional management of plant PIPs depends on four levels: (I) transcriptional level: the regulatable expression by some specific factors; (II) subcellular trafficking level: including exocytosis, endocytosis, autophagic degradation, and proteasomal degradation; (III) post-translational level: mainly referring to protein modifications; and (IV) gating regulation: including Ca\u003csup\u003e2+\u003c/sup\u003e ion levels, cytosolic pH, and reactive oxygen species (ROS) [\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e70\u003c/span\u003e]. Tables\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and \u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e show that the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome has more \u003cem\u003eAQP\u003c/em\u003e family members (58) than most other diploid species (except the paleotetraploid soybean). Further phylogenetic analysis (Figs.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e and \u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e) and collinearity analysis (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e) also demonstrated that most \u003cem\u003eTtAQP\u003c/em\u003e members were present in pairs. The increasing number of \u003cem\u003eTtAQP\u003c/em\u003es might be closely related to the morphological development and ecological adaptability of \u003cem\u003eT. tetragonoides\u003c/em\u003e.\u003c/p\u003e\u003cp\u003eConsidering that the regulation of gene expression is closely related to the biological functions of the genes, we examined the features of promoters and the expression patterns of \u003cem\u003eTtAQP\u003c/em\u003es. Previous research has revealed that abiotic stress, such as salt, drought, low temperature, dry air, ion, and temperature stress, in the surrounding environment causes water absorption difficulties in plants, thereby disturbing the water balance or even causing water deficit \u003cem\u003ein vivo\u003c/em\u003e and promoting \u003cem\u003eAQP\u003c/em\u003e expression [\u003cspan citationid=\"CR71\" class=\"CitationRef\"\u003e71\u003c/span\u003e]. Without exception, the predicted promoter regions of \u003cem\u003eTtAQPs\u003c/em\u003e contained multiple CEs related to abiotic stress responses (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e), including MYB- (including MBS) and MYC-recognition sites, HSEs, ABREs, and other hormone- and development-related CEs. The multiformity of CEs in \u003cem\u003eTtAQP\u003c/em\u003es\u0026rsquo; promoter regions confers the complexity and regulation of \u003cem\u003eTtAQPs\u003c/em\u003e\u0026rsquo; expression, making \u003cem\u003eT. tetragonoides\u003c/em\u003e plants more sensitive to ambient water conditions. In the present study, the RNA-seq assays were also performed with different \u003cem\u003eT. tetragonoides\u003c/em\u003e organs considering both their developmental functions (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e) and abiotic stress responses (Fig.\u0026nbsp;\u003cspan refid=\"Fig7\" class=\"InternalRef\"\u003e7\u003c/span\u003e). Based on these results, we conclude that some specific \u003cem\u003eTtAQP\u003c/em\u003e members were closely related to special development stages or organs of \u003cem\u003eT. tetragonoides\u003c/em\u003e, such as \u003cem\u003eTtPIP2;5\u003c/em\u003e/\u003cem\u003eTtPIP2;6\u003c/em\u003e in the roots and \u003cem\u003eTtTIP3;1\u003c/em\u003e/\u003cem\u003eTtTIP3;2\u003c/em\u003e in fruit (Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e). Additionally, the expression changes in \u003cem\u003eT. tetragonoides\u003c/em\u003e roots, stems, and leaves under different stress treatments also occurred in \u003cem\u003eTtPIP\u003c/em\u003e and \u003cem\u003eTtTIP\u003c/em\u003e subgroups (Figs.\u0026nbsp;\u003cspan refid=\"Fig7\" class=\"InternalRef\"\u003e7\u003c/span\u003e and \u003cspan refid=\"Fig8\" class=\"InternalRef\"\u003e8\u003c/span\u003e), suggesting that the \u003cem\u003eTtAQP\u003c/em\u003e members of these two subfamilies might play important roles in stress responses.\u003c/p\u003e\u003cp\u003eTo date, the \u003cem\u003eAQP\u003c/em\u003e gene family has been characterized in an increasing number of species; thus, we paid particular attention to this gene family in special habitat plant species [\u003cspan additionalcitationids=\"CR44 CR45 CR46 CR47 CR48 CR49 CR50\" citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e51\u003c/span\u003e]. Although the stress factors that caused the water imbalance can influence \u003cem\u003eAQP\u003c/em\u003e expression to varying degrees, with up- or down-regulation in different organs, further functional confirmation with transgenic overexpression in other plant species often presents different effects. Deeper functional research on plant \u003cem\u003eAQPs\u003c/em\u003e can be performed by reduced expression via antisense or RNA interference-mediated changes in the same plant species or by mutant \u003cem\u003eAQPs\u003c/em\u003e. Related transgenic research on plant \u003cem\u003eAQPs\u003c/em\u003e\u0026rsquo; role involved in salt and drought tolerance has emerged greatly in recent years. In particular, the \u003cem\u003eAQPs\u003c/em\u003e isolated from special habitat plants held our attention. For example, \u003cem\u003eSpAQP1\u003c/em\u003e (PIP2 group) from the halophyte \u003cem\u003eSesuvium portulacastrum\u003c/em\u003e increased salt tolerance in transgenic tobacco, probably by improving the efficiency of water transportation, thereby enhancing the antioxidative activity of transgenic plants [\u003cspan citationid=\"CR72\" class=\"CitationRef\"\u003e72\u003c/span\u003e]. \u003cem\u003eStipa purpurea\u003c/em\u003e better adapts to the stressors in the natural environment, such as drought and cold stress, and transgenic Arabidopsis overexpressing \u003cem\u003eSpPIP1\u003c/em\u003e (PIP1 group) presented enhanced drought (high osmotic) tolerance compared to WT control plants, indicating that this gene is a candidate gene for crop genetic improvement concerning drought tolerance [\u003cspan citationid=\"CR73\" class=\"CitationRef\"\u003e73\u003c/span\u003e]. Jojoba (\u003cem\u003eSimmondsia chinensi\u003c/em\u003e) is a typical desert plant with good tolerance to drought, salinity, and nutritional deficiency; when \u003cem\u003eScPIP1\u003c/em\u003e (PIP1 group) was overexpressed in Arabidopsis, the transgenic plants exhibited higher drought and salt tolerance than WT control plants [\u003cspan citationid=\"CR74\" class=\"CitationRef\"\u003e74\u003c/span\u003e]. Overexpression of \u003cem\u003eTsPIP1;1\u003c/em\u003e (PIP1 group) from \u003cem\u003eThellungiella salsuginea\u003c/em\u003e (also named \u003cem\u003eE. salsugineum\u003c/em\u003e) in rice increased its salt tolerance [\u003cspan citationid=\"CR75\" class=\"CitationRef\"\u003e75\u003c/span\u003e]. The expression of \u003cem\u003eSbPIP2\u003c/em\u003e (PIP2 group) in halophyte \u003cem\u003eSalicornia brachiata\u003c/em\u003e was rapidly induced after salt and high osmotic stress treatments; the over-expression of \u003cem\u003eSbPIP2\u003c/em\u003e in tobacco also enhanced the salt and high osmotic stress tolerance of transgenic plants [76]. We previously cloned two \u003cem\u003ePIP\u003c/em\u003es (\u003cem\u003eCrPIP1;5\u003c/em\u003e and \u003cem\u003eCrPIP2;3\u003c/em\u003e) from another tropical halophyte, \u003cem\u003eCanavalia rosea\u003c/em\u003e [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e, \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e], and performed transgenic overexpression assays in Arabidopsis. Unexpectedly, \u003cem\u003eCrPIP2;3\u003c/em\u003e enhanced the drought and salt tolerance of transgenic Arabidopsis, while \u003cem\u003eCrPIP1;5\u003c/em\u003e overexpression caused obviously higher drought and salt sensitivity in Arabidopsis plants, although their heterologous expression both caused slight sensitivities to high osmotic and salt stress in yeast cells. In this study, eight TtAQPs showed different tolerance or sensitivity changes to high salinity, osmotic stress (mimicking drought), and heat treatments in yeast (Figs.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003e and \u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003e), and their tolerance to H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e also showed obvious differences (Figs.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003eA and S4B). This is inconsistent with our previous research on plant \u003cem\u003ePIP\u003c/em\u003e gene expression in yeast [\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e, \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e], further indicating that the different plant species\u0026rsquo; AQPs might act in member-specific ways to regulate water or other small molecules or the interaction with other protein members and thereby playing specific roles in plants\u0026rsquo; abiotic stress responses. Further deep and systematic research on \u003cem\u003eTtAQP\u003c/em\u003es for abiotic stress-resistant genetic improvement in other microorganisms or crops is still required.\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eThis study presents the first genome-wide characterization of the AQP family in \u003cem\u003eT. tetragonoides\u003c/em\u003e and possible molecular mechanisms, identifying 58 \u003cem\u003eTtAQPs\u003c/em\u003e genes based on the genome sequencing data/through comprehensive genomic analysis. Through systematic analyses of evolutionary relationships, gene structure, gene duplication, promoters, and the possible regulatory factors of \u003cem\u003eTtAQP\u003c/em\u003es, we revealed differential expression patterns among TtAQP subfamilies, with TtPIPs and TtTIPs exhibiting particularly distinct regulatory profiles, suggesting their specialized functional roles in plant development and environmental stress responses of \u003cem\u003eT. tetragonoides\u003c/em\u003e. Functional validation through heterologous expression in different yeast strains demonstrated the potential involvement of selected eight candidate \u003cem\u003eTtAQP\u003c/em\u003es in stress response mechanisms. These finding provide a foundational framework for understanding the \u003cem\u003eAQP\u003c/em\u003e-mediated stress adaptation in \u003cem\u003eT. tetragonoides\u003c/em\u003e and offer valuable insights for future genetic improvement application of \u003cem\u003eTtAQP\u003c/em\u003es at enhancing stress tolerance in other plant species and microorganisms.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cdiv class=\"DefinitionList\"\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eAQP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eAquaporin\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eMIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eMajor intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eCE\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eCis-acting element\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eRT-PCR\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eReverse transcription-polymerase chain reaction\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003ePIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003ePlasma membrane intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eTIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eTonoplast intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eNIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eNOD26-like intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eSIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eSmall basic intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eXIP\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eUncategorized X intrinsic protein\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eTF\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eTranscription factor\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eWGD\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eWhole genome duplication\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eTD\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eTandem duplication\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003ePD\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eProximal duplication\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eTRD\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eTransposed duplication\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eDD\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eDispersed duplication\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv class=\"DefinitionListEntry\"\u003e\u003cdiv class=\"Term\"\u003eFPKM\u003c/div\u003e\u003cdiv class=\"Description\"\u003e\u003cp\u003eFragments per kilobase of transcript per million mapped reads\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eSupplementary Material\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe online version contains supplementary material available at https://doi.org/...\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgment\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor\u0026rsquo;s contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eLisha Cao: Data curation, Writing-original draft, Investigation. Fuying Xie: Data curation, Investigation. Lihua Chen: Investigation. Zhengfeng Wang: Data curation. Mei Zhang and Shuguang Jian: Writing-review and editing.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis research was funded by the Guangdong Science and Technology Program (No. 2024B1212050007), and the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (No. GML2019ZD0408). These funders had no roles in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll data generated or analyzed during this study are included in this published article [and its supplementary information file].\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNo particular approvals were necessary; materials were gathered in accordance with the national rules and under the supervision and consent of South China National Botanical Garden (SCNBG), Guangzhou, China, and all authors adhered to all local, national, and international guidelines and legislation in accordance with the rules established by SCNBG.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eAtzori G, Nissim W, Macchiavelli T, Vita F, Azzarello E, Pandolfi C, Masi E, Stefano Mancuso S. \u003cem\u003eTetragonia tetragonioides\u003c/em\u003e (pallas) kuntz. as promising salt-tolerant crop in a saline agricultural context. Agr Water Manage. 2020;240:106261.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLai XK, Lin NX, Chen JZ, Huang L, Ji JF, Chen RH, Liang WQ, Wu JX. Studies on cultivation trial of salt-tolerant New Zealand spinach in Quanzhou bay (In Chinese). Fujian Agri Sci Tech. 2016;1:24\u0026ndash;7.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLi RX, Nie WJ, Li B, Li JL, Wang XY, Qiao P, Fu R. Purification effects of \u003cem\u003eTetragonia tetragonoides\u003c/em\u003e on seawater with different salinity and eutrophication degrees (In Chinese). Shandong Agri Sci. 2022;54(3):99\u0026ndash;105.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eChoi HS, Cho JY, Kim SJ, Ham KS, Moon JH. New lignan tyramide, phenolics, megastigmanes, and their glucosides from aerial parts of New Zealand spinach, \u003cem\u003eTetragonia tetragonoides\u003c/em\u003e. Food Sci Biotechnol. 2019;29(5):599\u0026ndash;608.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eChen X. Why are there sudden instances of seawater inundation in many places in China[N]. Sci Technol Dly. 2024-10\u0026ndash;31.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eXu WF. Researches on recovery the seawater-intrusion fields \u0026amp; plant characteristics under seawater stress of \u003cem\u003eMesembryanthemum crystallinum\u003c/em\u003e L. Hainan: Hainan University. 2018;4\u0026ndash;6.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eNeves MA, Miguel MG, Panagapoulos T, Beltrao J. Salt removing species-an environmentally safe and clean technique to control salinity[M]. Salt Removing Species. 2014;1\u0026ndash;11.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWilson C, Lesch SM, Grieve CM. Growth stage modulates salinity tolerance of New Zealand spinach (\u003cem\u003eTetragonia tetragonioides\u003c/em\u003e Pall.) and red orach (\u003cem\u003eAtriplex hortensis\u003c/em\u003e L). Ann Bot. 2000;85:501\u0026ndash;9.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eYousif BS, Nguyen NT, Fukuda Y, Hakata H, Okamoto Y, Masaoka Y, Saneoka H. Effect of salinity on growth, mineral composition, photosynthesis and water relations of two vegetable crops; New Zealand spinach (\u003cem\u003eTetragonia tetragonioides\u003c/em\u003e) and water spinach (\u003cem\u003eIpomoea aquatica\u003c/em\u003e). Int J Agric Biol. 2010;12:211\u0026ndash;6.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eQin S, Liu Y, Han Y, Xu G, Wan S, Cui F, Li G. Aquaporins and their function in root water transport under salt stress conditions in \u003cem\u003eEutrema salsugineum\u003c/em\u003e. Plant Sci. 2019;287:110199.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eNongpiur RC, Singla-Pareek SL, Pareek A. The quest for osmosensors in plants. J Exp Bot. 2020;71(2):595\u0026ndash;607.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eByrt CS, Zhang RY, Magrath I, Chan KX, De Rosa A, McGaughey S. Exploring aquaporin functions during changes in leaf water potential. Front Plant Sci. 2023;14:1213454.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMaurel C, Tournaire-Roux C, Verdoucq L, Santoni V. Hormonal and environmental signaling pathways target membrane water transport. Plant Physiol. 2021;187(4):2056\u0026ndash;70.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eIshibashi K, Kondo S, Hara S, Morishita Y. The evolutionary aspects of aquaporin family. Am J Physiol Regul Integr Comp Physiol. 2011;300(3):R566\u0026ndash;76.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKaldenhoff R, Ribas-Carbo M, Sans JF, Lovisolo C, Heckwolf M, Uehlein N. Aquaporins and plant water balance. Plant Cell Environ. 2008;31(5):658\u0026ndash;66.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMukherjee S, Roy S, Corpas FJ. Aquaporins: a vital nexus in H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e-gasotransmitter signaling. Trends Plant Sci. 2024;29(6):681\u0026ndash;93.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eTakano J, Wada M, Ludewig U, Schaaf G, von Wir\u0026eacute;n N, Fujiwara T. The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation. Plant Cell. 2006;18:1498\u0026ndash;509.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMa JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M. A silicon transporter in rice. Nature. 2006;440:688\u0026ndash;91.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eBienert GP, Thorsen M, Sch\u0026uuml;ssler MD, Nilsson HR, Wagner A, Tam\u0026aacute;s MJ, Jahn TP. A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)\u003csub\u003e3\u003c/sub\u003e and Sb(OH)\u003csub\u003e3\u003c/sub\u003e across membranes. BMC Biol. 2008;6:26.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZhao XQ, Mitani NM, Yamaji N, Shen RS, Ma JF. Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice. Plant Physiol. 2010;153:1871\u0026ndash;7.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMitani-Ueno N, Yamaji N, Zhao FJ, Ma JF. The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic. J Exp Bot. 2011;62:4391\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSrivastava AK, Penna S, Nguyen DV, Tran LS. Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses. Crit Rev Biotechnol. 2016;36(3):389\u0026ndash;98.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eDu B, Franzisky BL, Muhammad W, Alfarraj S, Geilfus CM, Rennenberg H. How to cope with stress in the desert-the date palm approach. Plant Cell Environ. 2025;48(1):768\u0026ndash;80.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eTarolli P, Luo J, Park E, Barcaccia G, Masin R. Soil salinization in agriculture: mitigation and adaptation strategies combining nature-based solutions and bioengineering. iScience. 2024;27(2):108830.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZargar SM, Nagar P, Deshmukh R, Nazir M, Wani AA, Masoodi KZ, Agrawal GK, Rakwal R. Aquaporins as potential drought tolerance inducing proteins: towards instigating stress tolerance. J Proteom. 2017;169:233\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMartinez-Ballesta Mdel C, Carvajal M. New challenges in plant aquaporin biotechnology. Plant Sci. 2014;217\u0026ndash;218:71\u0026ndash;7.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eJang JY, Lee SH, Rhee JY, Chung GC, Ahn SJ, Kang H. Transgenic Arabidopsis and tobacco plants overexpressing an aquaporin respond differently to various abiotic stresses. Plant Mol Biol. 2007;64(6):621\u0026ndash;32.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZhou L, Wang C, Liu R, Han Q, Vandeleur RK, Du J, Tyerman S, Shou H. Constitutive overexpression of soybean plasma membrane intrinsic protein \u003cem\u003eGmPIP1;6\u003c/em\u003e confers salt tolerance. BMC Plant Biol. 2014;14:181.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSreedharan S, Shekhawat UK, Ganapathi TR. Transgenic banana plants overexpressing a native plasma membrane aquaporin \u003cem\u003eMusaPIP1;2\u003c/em\u003e display high tolerance levels to different abiotic stresses. Plant Biotechnol J. 2013;11(8):942\u0026ndash;52.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSreedharan S, Shekhawat UK, Ganapathi TR. Constitutive and stress-inducible overexpression of a native aquaporin gene (\u003cem\u003eMusaPIP2;6\u003c/em\u003e) in transgenic banana plants signals its pivotal role in salt tolerance. Plant Mol Biol. 2015;88(1\u0026ndash;2):41\u0026ndash;52.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eAlavilli H, Awasthi JP, Rout GR, Sahoo L, Lee BH, Panda SK. Overexpression of a barley aquaporin gene, \u003cem\u003eHvPIP2;5\u003c/em\u003e confers salt and osmotic stress tolerance in yeast and plants. Front Plant Sci. 2016;7:1566.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLi R, Wang J, Li S, Zhang L, Qi C, Weeda S, Zhao B, Ren S, Guo YD. Plasma membrane intrinsic proteins SlPIP2;1, SlPIP2;7 and SlPIP2;5 conferring enhanced drought stress tolerance in tomato. Sci Rep. 2016;6:31814.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWang L, Li QT, Lei Q, Feng C, Zheng X, Zhou F, Li L, Liu X, Wang Z, Kong J. Ectopically expressing \u003cem\u003eMdPIP1;3\u003c/em\u003e, an aquaporin gene, increased fruit size and enhanced drought tolerance of transgenic tomatoes. BMC Plant Biol. 2017;17(1):246.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMartins CP, Neves DM, Cidade LC, Mendes AF, Silva DC, Almeida AF, Coelho-Filho MA, Gesteira AS, Soares-Filho WS, Costa MG. Expression of the citrus \u003cem\u003eCsTIP2;1\u003c/em\u003e gene improves tobacco plant growth, antioxidant capacity and physiological adaptation under stress conditions. Planta. 2017;245(5):951\u0026ndash;63.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eAyadi M, Brini F, Masmoudi K. Overexpression of a wheat aquaporin gene, \u003cem\u003eTdPIP2;1\u003c/em\u003e, enhances salt and drought tolerance in transgenic durum wheat cv. Maali. Int J Mol Sci. 2019;20(10):2389.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWang Y, Zhang Y, An Y, Wu J, He S, Sun L, Hao F. Wheat \u003cem\u003eTaTIP4;1\u003c/em\u003e confers enhanced tolerance to drought, salt and osmotic stress in Arabidopsis and rice. Int J Mol Sci. 2022;23(4):2085.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWang X, Li Y, Ji W, Bai X, Cai H, Zhu D, Sun XL, Chen LJ, Zhu YM. A novel \u003cem\u003eGlycine soja\u003c/em\u003e tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic \u003cem\u003eArabidopsis thaliana\u003c/em\u003e. J Plant Physiol. 2011;168(11):1241\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWang X, Cai H, Li Y, Zhu Y, Ji W, Bai X, Zhu D, Sun X. Ectopic overexpression of a novel \u003cem\u003eGlycine soja\u003c/em\u003e stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic \u003cem\u003eArabidopsis thaliana\u003c/em\u003e plants. J Plant Res. 2015;128(1):103\u0026ndash;13.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLi J, Yu G, Sun X, Liu Y, Liu J, Zhang X, Jia C, Pan H. AcPIP2, a plasma membrane intrinsic protein from halophyte \u003cem\u003eAtriplex canescens\u003c/em\u003e, enhances plant growth rate and abiotic stress tolerance when overexpressed in \u003cem\u003eArabidopsis thaliana\u003c/em\u003e. Plant Cell Rep. 2015;34(8):1401\u0026ndash;15.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLin R, Zheng J, Pu L, Wang Z, Mei Q, Zhang M, Jian S. Genome-wide identification and expression analysis of \u003cem\u003eaquaporin\u003c/em\u003e family in \u003cem\u003eCanavalia rosea\u003c/em\u003e and their roles in the adaptation to saline-alkaline soils and drought stress. BMC Plant Biol. 2021;21(1):333.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZheng J, Lin R, Pu L, Wang Z, Mei Q, Zhang M, Jian S. Ectopic expression of \u003cem\u003eCrPIP2;3\u003c/em\u003e, a plasma membrane intrinsic protein gene from the halophyte \u003cem\u003eCanavalia rosea\u003c/em\u003e, enhances drought and salt-alkali stress tolerance in Arabidopsis. Int J Mol Sci. 2021;22(2):565.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eShekoofa A, Sinclair TR. Aquaporin activity to improve crop drought tolerance. Cells. 2018;7(9):123.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eQian W, Yang X, Li J, Luo R, Yan X, Pang Q. Genome-wide characterization and expression analysis of aquaporins in salt cress (\u003cem\u003eEutrema salsugineum\u003c/em\u003e). PeerJ. 2019;7:e7664.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eFaize M, Fumanal B, Luque F, Ram\u0026iacute;rez-Tejero JA, Zou Z, Qiao X, Faize L, Gousset-Dupont A, Roeckel-Drevet P, Label P, Venisse JS. Genome wild analysis and molecular understanding of the aquaporin diversity in olive trees (\u003cem\u003eOlea Europaea\u003c/em\u003e L). Int J Mol Sci. 2020;21(11):4183.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eKumar N, Kumawat S, Khatri P, Singla P, Tandon G, Bhatt V, Shinde S, Patil GB, Sonah H, Deshmukh R. Understanding aquaporin transport system in highly stress-tolerant and medicinal plant species Jujube (\u003cem\u003eZiziphus jujuba\u003c/em\u003e Mill). J Biotechnol. 2020;324:103\u0026ndash;11.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGrondin A, Affortit P, Tranchant-Dubreuil C, de la Fuente-Cant\u0026oacute; C, Mariac C, Gantet P, Vadez V, Vigouroux Y, Laplaze L. Aquaporins are main contributors to root hydraulic conductivity in pearl millet [\u003cem\u003ePennisetum glaucum\u003c/em\u003e (L) R. Br]. PLoS ONE. 2020;15(10):e0233481.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZhang GH, Yu ZM, Teixeira da Silva JA, Wen DZ. Identification of \u003cem\u003eaquaporin\u003c/em\u003e members in \u003cem\u003eAcacia auriculiformis\u003c/em\u003e and functional characterization of \u003cem\u003eAaPIP1-2\u003c/em\u003e involved in drought stress. Environ Exp Bot. 2021;185:104425.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eHe W, Liu M, Qin X, Liang A, Chen Y, Yin Y, Qin K, Mu Z. Genome-wide identification and expression analysis of the \u003cem\u003eaquaporin\u003c/em\u003e gene family in \u003cem\u003eLycium barbarum\u003c/em\u003e during fruit ripening and seedling response to heat stress. Curr Issues Mol Biol. 2022;44(12):5933\u0026ndash;48.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGuo Z, Ma D, Li J, Wei M, Zhang L, Zhou L, Zhou X, He S, Wang L, Shen Y, Li QQ, Zheng HL. Genome-wide identification and characterization of aquaporins in mangrove plant \u003cem\u003eKandelia obovata\u003c/em\u003e and its role in response to the intertidal environment. Plant Cell Environ. 2022;45(6):1698\u0026ndash;718.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGuo Z, Wei M, Xu C, Wang L, Li J, Liu J, Zhong Y, Chi B, Song S, Zhang L, Song L, Ma D, Zheng HL. Genome-wide identification of \u003cem\u003eAvicennia marina\u003c/em\u003e aquaporins reveals their role in adaptation to intertidal habitats and their relevance to salt secretion and vivipary. Plant Cell Environ. 2024;47(3):832\u0026ndash;53.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eNdayambaza B, Si J, Zhou D, Bai X, Jia B, He X, Wang C, Qin J, Zhu X, Liu Z, Wang B. Genome-wide analysis of aquaporins gene family in \u003cem\u003ePopulus euphratica\u003c/em\u003e and its expression patterns in response to drought, salt stress, and phytohormones. Int J Mol Sci. 2024;25(18):10185.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eHuang Z, Ding Q, Wang Z, Jian S, Zhang M. Genome-wide identification and expression analyses of the thaumatin-like protein gene family in \u003cem\u003eTetragonia tetragonoides\u003c/em\u003e (Pall.) Kuntze reveal their functions in abiotic stress responses. Plants (Basel). 2024;13(17):2355.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eChen C, Chen H, Zhang Y, Thomas HR, Frank MH, He Y, Xia R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant. 2020;13(8):1194\u0026ndash;202.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eNguyen MX, Moon S, Jung KH. Genome-wide expression analysis of rice aquaporin genes and development of a functional gene network mediated by aquaporin expression in roots. Planta. 2013;238(4):669\u0026ndash;81.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eNei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986;3(5):418\u0026ndash;26.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMaruyama K, Ogata T, Kanamori N, Yoshiwara K, Goto S, Yamamoto YY, Tokoro Y, Noda C, Takaki Y, Urawa H, Iuchi S, Urano K, Yoshida T, Sakurai T, Kojima M, Sakakibara H, Shinozaki K, Yamaguchi-Shinozaki K. Design of an optimal promoter involved in the heat-induced transcriptional pathway in Arabidopsis, soybean, rice and maize. Plant J. 2017;89(4):671\u0026ndash;80.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eLiu H, Ding Q, Cao L, Huang Z, Wang Z, Zhang M, Jian S. Identification of the \u003cem\u003eAbscisic acid-, Stress-, and Ripening-Induced\u003c/em\u003e (\u003cem\u003eASR\u003c/em\u003e) family involved in the adaptation of \u003cem\u003eTetragonia tetragonoides\u003c/em\u003e (Pall.) Kuntze to saline-alkaline and drought habitats. Int J Mol Sci. 2023;24(21):15815.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eOffringa R, Huang F. Phosphorylation-dependent trafficking of plasma membrane proteins in animal and plant cells. J Integr Plant Biol. 2013;55(9):789\u0026ndash;808.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eAhmed S, Kouser S, Asgher M, Gandhi SG. Plant aquaporins: a frontward to make crop plants drought resistant. Physiol Plant. 2021;172(2):1089\u0026ndash;105.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWu HJ, Zhang Z, Wang JY, Oh DH, Dassanayake M, Liu B, Huang Q, Sun HX, Xia R, Wu Y, Wang YN, Yang Z, Liu Y, Zhang W, Zhang H, Chu J, Yan C, Fang S, Zhang J, Wang Y, Zhang F, Wang G, Lee SY, Cheeseman JM, Yang B, Li B, Min J, Yang L, Wang J, Chu C, Chen SY, Bohnert HJ, Zhu JK, Wang XJ, Xie Q. Insights into salt tolerance from the genome of \u003cem\u003eThellungiella salsuginea\u003c/em\u003e. Proc Natl Acad Sci USA. 2012;109(30):12219\u0026ndash;24.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGolldack D, Li C, Mohan H, Probst N. Tolerance to drought and salt stress in plants: unraveling the signaling networks. Front Plant Sci. 2014;5:151.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eMaurel C, Boursiac Y, Luu DT, Santoni V, Shahzad Z, Verdoucq L. Aquaporins in plants. Physiol Rev. 2015;95(4):1321\u0026ndash;58.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGroszmann M, De Rosa A, Chen W, Qiu J, McGaughey SA, Byrt CS, Evans JR. A high-throughput yeast approach to characterize aquaporin permeabilities: profiling the Arabidopsis PIP aquaporin sub-family. Front Plant Sci. 2023;14:1078220.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eCu\u0026eacute;llar-Cruz M, Briones-Martin-del-Campo M, Ca\u0026ntilde;as-Villamar I, Montalvo-Arredondo J, Riego-Ruiz L, Casta\u0026ntilde;o I, De Las Pe\u0026ntilde;as A. High resistance to oxidative stress in the fungal pathogen \u003cem\u003eCandida glabrata\u003c/em\u003e is mediated by a single catalase, Cta1p, and is controlled by the transcription factors Yap1p, Skn7p, Msn2p, and Msn4p. Eukaryot Cell. 2008;7(5):814\u0026ndash;25.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eZhang M, Wang Z, Jian S. Functional characterization of \u003cem\u003eheat shock factor\u003c/em\u003e (\u003cem\u003eCrHsf\u003c/em\u003e) families provide comprehensive insight into the adaptive mechanisms of \u003cem\u003eCanavalia rosea\u003c/em\u003e (Sw.) DC. to tropical coral islands. Int J Mol Sci. 2022;23(20):12357.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eShabala S, Bose J, Hedrich R. Salt bladders: do they matter? Trends Plant Sci. 2014;19(11):687\u0026ndash;91.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eGuo H, Zhang L, Cui YN, Wang SM, Bao AK. Identification of candidate genes related to salt tolerance of the secretohalophyte Atriplex canescens by transcriptomic analysis. BMC Plant Biol. 2019;19(1):213.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eG\u0026oacute;mez-M\u0026eacute;ndez MF, Amezcua-Romero JC, Rosas-Santiago P, Hern\u0026aacute;ndez-Dom\u0026iacute;nguez EE, de Luna-Valdez LA, Ruiz-Salas JL, Vera-Estrella R, Pantoja O. Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress. Plant Physiol. 2023;191(1):199\u0026ndash;218.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSharma R, Kumar D, Parkirti P, Singh A, Sharma A, Langeh K, Singh A, Sharma M, Mir NR, Kapoor A, Bhardwaj N, Ohri R. Membrane transporters in plants: key players in abiotic and biotic stress tolerance and nutritional transport. Plant Physiol Bioch. 2025;227:110084.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eDahuja A, Kumar RR, Sakhare A, Watts A, Singh B, Goswami S, Sachdev A, Praveen S. Role of ATP-binding cassette transporters in maintaining plant homeostasis under abiotic and biotic stresses. Physiol Plant. 2021;171(4):785\u0026ndash;801.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eSutka M, Li G, Boudet J, Boursiac Y, Doumas P, Maurel C. Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions. Plant Physiol. 2011;155(3):1264\u0026ndash;76.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eChang W, Liu X, Zhu J, Fan W, Zhang Z. An aquaporin gene from halophyte \u003cem\u003eSesuvium portulacastrum\u003c/em\u003e, \u003cem\u003eSpAQP1\u003c/em\u003e, increases salt tolerance in transgenic tobacco. Plant Cell Rep. 2016;35(2):385\u0026ndash;95.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eChen Q, Yang S, Kong X, Wang C, Xiang N, Yang Y, Yang Y. Molecular cloning of a plasma membrane aquaporin in \u003cem\u003eStipa purpurea\u003c/em\u003e, and exploration of its role in drought stress tolerance. Gene. 2018;665:41\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003eWang X, Gao F, Bing J, Sun W, Feng X, Ma X, Zhou Y, Zhang G. Overexpression of the jojoba aquaporin gene, \u003cem\u003eScPIP1\u003c/em\u003e, enhances drought and salt tolerance in transgenic Arabidopsis. Int J Mol Sci. 2019;20(1):153.\u003c/span\u003e\u003c/li\u003e\u003cli\u003e\u003cspan\u003ePatel J, Khatri K, Khandwal D, Gupta NK, Choudhary B, Hapani D, Koshiya J, Syed SN, Phillips DW, Jones HD, Mishra A. Modulation of physio-biochemical and photosynthesis parameters by overexpressing \u003cem\u003eSbPIP2\u003c/em\u003e gene improved abiotic stress tolerance of transgenic tobacco. Physiol Plant. 2024;176(3):e14384.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-plant-biology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pbio","sideBox":"Learn more about [BMC Plant Biology](http://bmcplantbiol.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/pbio/default.aspx","title":"BMC Plant Biology","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Aquaporins (AQPs), Abiotic stress, Ecological adaptability, Tetragonia tetragonoides","lastPublishedDoi":"10.21203/rs.3.rs-7136705/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7136705/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eTetragonia tetragonoides\u003c/em\u003e (Pall.) Kuntze (Aizoaceae, 2n\u0026thinsp;=\u0026thinsp;2x\u0026thinsp;=\u0026thinsp;32) is a secretohalophyte with excellent tolerance to high salinity\u0026ndash;alkalinity, drought, and heat stress. As a medicinal and edible vegetable, \u003cem\u003eT. tetragonoides\u003c/em\u003e is widely distributed in the coastal tropics and subtropics worldwide. Aquaporin (AQP) proteins, belonging to the major intrinsic protein (MIP) superfamily, are water channel proteins that facilitate the transport of water and other substrates across cell membranes. AQPs play important roles in physiological processes, including water transport, nutrient acquisition (carbon, nitrogen, and micronutrients), and abiotic stress responses. However, knowledge of AQPs in the special habitat plant \u003cem\u003eT. tetragonoides\u003c/em\u003e is limited. In this study, we identified 58 candidate \u003cem\u003eTtAQP\u003c/em\u003e genes in the \u003cem\u003eT. tetragonoides\u003c/em\u003e genome and classified them into five subfamilies based on phylogenetic analysis with 15 plasma membrane intrinsic proteins (PIPs), 18 tonoplast intrinsic proteins (TIPs), 19 NOD26-like intrinsic proteins (NIPs), 4 small basic intrinsic proteins (SIPs) and 2 uncategorized X intrinsic proteins (XIPs). Gene structure and protein conserved domain analyses showed that the majority of the deduced TtAQPs contained signature transmembrane domains, NPA motifs, the ar/R selectivity filter, and Froger\u0026rsquo;s positions, suggesting substrate specificity for these TtAQPs. Analyses of \u003cem\u003ecis\u003c/em\u003e-acting elements (CEs) in \u003cem\u003eTtAQP\u003c/em\u003es\u0026rsquo; promoter regions revealed the presence of stress-responsive and hormone responsive elements, indicating complex regulatory mechanisms for the expression of \u003cem\u003eTtAQP\u003c/em\u003es. \u003cem\u003eTtAQP\u003c/em\u003es exhibited different expression patterns among tissues and under different stress conditions based on RNA sequencing and quantitative reverse transcription PCR assays. Several \u003cem\u003eTtAQP\u003c/em\u003es were cloned and heterologously expressed in yeast to confirm the stress tolerance conferred by the overexpression of these genes. Our findings provide a comprehensive framework for further functional studies of \u003cem\u003eTtAQP\u003c/em\u003es and their potential applications in crop genetic improvement. The results also enhance our understanding of the ecological adaptability of \u003cem\u003eT. tetragonoides\u003c/em\u003e to extremely harsh environments and offer valuable insights for developing stress-tolerant transgenic plants through genetic engineering techniques.\u003c/p\u003e","manuscriptTitle":"Molecular characterization of Tetragonia tetragonoides (Pall.) aquaporin (AQP) members and their roles in the response to combined high salinity-alkalinity-drought and heat stress","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-07-30 12:35:40","doi":"10.21203/rs.3.rs-7136705/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-08-18T04:15:33+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-08-15T03:59:54+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-08-11T07:46:25+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"4901279841401088598640980584496404807","date":"2025-07-31T16:21:18+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"262870844504744509998316267204515202642","date":"2025-07-31T08:22:31+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"107188399233954641930068793852866792312","date":"2025-07-29T01:55:03+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-07-29T00:41:28+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-07-29T00:39:30+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-07-23T14:01:37+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-07-23T01:07:45+00:00","index":"","fulltext":""},{"type":"submitted","content":"BMC Plant Biology","date":"2025-07-23T01:02:14+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bmc-plant-biology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pbio","sideBox":"Learn more about [BMC Plant Biology](http://bmcplantbiol.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/pbio/default.aspx","title":"BMC Plant Biology","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"9e58c072-7517-412c-9226-acd8859d857e","owner":[],"postedDate":"July 30th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-11-03T16:03:39+00:00","versionOfRecord":{"articleIdentity":"rs-7136705","link":"https://doi.org/10.1186/s12870-025-07410-z","journal":{"identity":"bmc-plant-biology","isVorOnly":false,"title":"BMC Plant Biology"},"publishedOn":"2025-10-31 15:58:39","publishedOnDateReadable":"October 31st, 2025"},"versionCreatedAt":"2025-07-30 12:35:40","video":"","vorDoi":"10.1186/s12870-025-07410-z","vorDoiUrl":"https://doi.org/10.1186/s12870-025-07410-z","workflowStages":[]},"version":"v1","identity":"rs-7136705","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7136705","identity":"rs-7136705","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}