Inhibitory and protective effect of obovatol against uterine fibroid (leiomyoma) cells.

Ling Chen, Qingxiang Hou, Feng Li, Ling Xin, Li Ma
In: Pakistan journal of pharmaceutical sciences · 2020 · vol. 33(1(Supplementary)) , pp. 281–285 · PMID:32122859 · W3013369098
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Obovatol treatment of human leiomyoma cells significantly reduced proliferation, inflammatory cytokines, and upregulated apoptosis markers, suggesting potential chemotherapeutic properties.

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The study examined the anti-proliferative and pro-apoptotic effects of obovatol (OB) on human uterine leiomyoma (fibroid) cells (HuLM) using MTT assays, measurements of inflammatory cytokines (IL-1β, TNF-α, IL-6), ELISA-based DNA fragmentation and apoptotic markers, and immunoblotting of apoptosis-regulatory proteins. Increasing concentrations of OB (25–200 μM) significantly reduced HuLM cell proliferation and lowered pro-inflammatory cytokine levels, while increasing DNA fragmentation and apoptotic marker activity, including elevated caspase-3 and caspase-9. OB also down-regulated Bcl2 and up-regulated Bax in a dose-dependent manner. The paper notes that further studies are required before OB can be recommended for treating uterine fibroid subjects. This paper is centrally about adenomyosis? No—this paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Uterine fibroids (UF) or leiomyomas can be presented in post-menopausal women. The present study was aimed to examine the inhibitory and protective (anti-proliferative and apoptotic) effect of the obovatol (OB) in human leiomyoma cells (HuLM). The cell proliferative activity was determined by MTT assay and inflammatory markers were measured. Followed by evaluating DNA fragmentation and apoptotic markers using the ELISA kit method. Also, the apoptosis regulatory proteins expressions were determined using the immunoblot technique. Treatment with increasing concentration of OB (25-200 μM) significantly lowered the cell proliferation rate as well as considerably reduced the values of various pro-inflammatory cytokines like IL-1β, TNF-α, IL-6. Whereas, the levels of DNA fragmentation and apoptotic marker like caspase-3 and 9 were considerably elevated after co-culturing HuLM cells with OB. In addition, apoptosis regulatory proteins like Bcl2 and Bax were substantially down and up-regulated respectively, by OB in a dose-dependent fashion. The above data clearly showcase that OB possesses potent anti-proliferative (inhibitory) as well as apoptotic activity and may be recommended as a chemotherapeutic agent against UF and related conditions. However, further studies are required before recommended for treating UF subjects.
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Inhibitory and protective effect of obovatol against uterine fibroid (leiomyoma) cells - PMID: 32122859 Inhibitory and protective effect of obovatol against uterine fibroid (leiomyoma) cells Abstract Uterine fibroids (UF) or leiomyomas can be presented in post-menopausal women. The present study was aimed to examine the inhibitory and protective (anti-proliferative and apoptotic) effect of the obovatol (OB) in human leiomyoma cells (HuLM). The cell proliferative activity was determined by MTT assay and inflammatory markers were measured. Followed by evaluating DNA fragmentation and apoptotic markers using the ELISA kit method. Also, the apoptosis regulatory proteins expressions were determined using the immunoblot technique. Treatment with increasing concentration of OB (25-200 μM) significantly lowered the cell proliferation rate as well as considerably reduced the values of various pro-inflammatory cytokines like IL-1β, TNF-α, IL-6. Whereas, the levels of DNA fragmentation and apoptotic marker like caspase-3 and 9 were considerably elevated after co-culturing HuLM cells with OB. In addition, apoptosis regulatory proteins like Bcl2 and Bax were substantially down and up-regulated respectively, by OB in a dose-dependent fashion. The above data clearly showcase that OB possesses potent anti-proliferative (inhibitory) as well as apoptotic activity and may be recommended as a chemotherapeutic agent against UF and related conditions. However, further studies are required before recommended for treating UF subjects. MeSH terms Substances LinkOut - more resources Medical Research Materials Miscellaneous

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