The detection of apoptosis using BimEL staining in primordial and growing follicles in endometriosis patients.

JUNICHI YOSHIHIRO, Yoshihiro J. Ono (678774), Akiko Tanabe, Yoko Nakamura, Hikaru Yamamoto, Atsushi Hayashi, Tomohito Tanaka, Hiroshi Sasaki, Masami Hayashi, Yoshito Terai, Ohmichi Masahide, Masahide Ohmichi
2015 · doi:10.1371/journal.pone.0115618.g001 · W2137077611
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⚙ AI-generated summary by claude@2026-06, 2026-06-10 ⓘ

This study quantified BimEL staining in ovarian follicles from endometriosis patients, categorizing specimens as BimEL-positive (>20% positive follicles) or BimEL-negative (<20% positive follicles).

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 · read from full text ⓘ

The paper studied apoptosis in ovarian follicles from endometriosis patients by examining BimEL staining in primordial and growing follicles using sequential immunohistochemical sections, with granulosa-cell apoptosis scored as BimEL-positive when specific staining was present in almost all granulosa cells. The authors quantified the BimEL-positive rate across multiple fields per specimen, counting at least 100 follicles per patient, and classified specimens as BimEL-positive when the rate exceeded 20%. The key finding presented is the detection of BimEL-associated apoptosis signals in granulosa cells within primordial and growing follicles in endometriosis patients, using this threshold-based staining approach. The main limitation explicitly reflected in the methodology is the reliance on a categorical cut-off and semi-quantitative scoring based on staining presence, rather than a fully continuous apoptosis measure. This paper is centrally about endometriosis — it specifically evaluates apoptosis in primordial and growing ovarian follicles of endometriosis patients using BimEL staining.

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Abstract

Representative examples of sequential immunohistochemical sections stained with hematoxylin and eosin (b and e), anti-BimEL Abs (a, d, and g) and anti-FSH receptor Ab (c and f). The granulosa cells of each follicle were scored “negative” if there was absolutely no staining for BimEL, or if staining was present in only some granulosa cells. Follicles were scored “positive” if specific BimEL staining was present in almost all granulosa cells. The ratio of the number of follicles positively stained for BimEL to the total number of primordial and growing follicles was determined in six different fields in each specimen. At least total 100 follicles were counted in each patient. The specimens with a BimEL-positive rate higher than 20% was classified as the BimEL-positive group (panel B), and the specimens with staining of less than 20% of the specimen were classified as the BimEL-negative group (panel A).
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A Low-Testosterone State Associated with Endometrioma Leads to the Apoptosis of Granulosa Cells Figure 1 The detection of apoptosis using BimEL staining in primordial and growing follicles in endometriosis patients. Representative examples of sequential immunohistochemical sections stained with hematoxylin and eosin (b and e), anti-BimEL Abs (a, d, and g) and anti-FSH receptor Ab (c and f). The granulosa cells of each follicle were scored “negative” if there was absolutely no staining for BimEL, or if staining was present in only some granulosa cells. Follicles were scored “positive” if specific BimEL staining was present in almost all granulosa cells. The ratio of the number of follicles positively stained for BimEL to the total number of primordial and growing follicles was determined in six different fields in each specimen. At least total 100 follicles were counted in each patient. The specimens with a BimEL-positive rate higher than 20% was classified as the BimEL-positive group (panel B), and the specimens with staining of less than 20% of the specimen were classified as the BimEL-negative group (panel A).

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endometriosis

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