Pan-genomic and genetic dissection of drought tolerance in a resilient wheat germplasm JIN50

Pan-genomic and genetic dissection of drought tolerance in a resilient wheat germplasm JIN50 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Pan-genomic and genetic dissection of drought tolerance in a resilient wheat germplasm JIN50 Zhaorong Hu, Jingchen Lin, Chenji Zhang, Zehui Liu, Jinpeng Li, and 15 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6539185/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Global climate change intensifies drought threats to wheat productivity, necessitating genetic enhancement of drought resistance. We present a high-quality genome assembly of drought-resistant wheat genotype JIN50 (contig N50 = 50.63 Mb) and conduct pan-genomic analyses across 14 wheat genomes and 196 germplasms, revealing 430,739 structural variants (SVs). Integrated SV-, SNP-, and Indel-based GWAS identified 46 drought-resistance loci enriched in JIN50. Notably, the structural variation in promoter of root development regulator TaLBD1 and functional haplotypes of methylglyoxal detoxification enzyme TaGLYI7, both contributing to drought adaptation through distinct mechanisms. The JIN50 genome and pan-genomic resources provide critical tools for deciphering drought-resistance networks and accelerating molecular breeding. These findings advance understanding of wheat's multigenic drought response system and offer strategic genomic solutions to bolster food security under climate challenges. Biological sciences/Plant sciences/Plant genetics Biological sciences/Biotechnology/Functional genomics Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryTables119.xlsx Supplementary Tables 1-19 ExtendedDataFig.19.pdf Extended Data Fig.1-9 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6539185","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":457206171,"identity":"f42aa5d0-3f0a-4f9d-9a53-212e1ab42a83","order_by":0,"name":"Zhaorong 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