Diversity of endophytic bacteria with antimicrobial potential isolated from marine macroalgae from Yacila and Cangrejos beaches, Piura-Peru

Diversity of endophytic bacteria with antimicrobial potential isolated from marine macroalgae from Yacila and Cangrejos beaches, Piura-Peru | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Diversity of endophytic bacteria with antimicrobial potential isolated from marine macroalgae from Yacila and Cangrejos beaches, Piura-Peru Edwin Jorge Vega Portalatino, Miriam Marleni Rosales Cuentas, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4326823/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Aug, 2024 Read the published version in Archives of Microbiology → Version 1 posted 10 You are reading this latest preprint version Abstract Endophytic bacteria from marine macroalgae (EBMM) have aroused interest for their antimicrobial activity, becoming a new source of bioactive compounds for the control of pathogenic bacteria, yeasts and fungi. EBMMs were isolated from Caulerpa sp., Ulva sp., Ahnfeltiopsis sp. and Chondracantus chamissoi collected from Yacila and Cangrejo beach (Piura, Peru). Antimicrobial assays against pathogenic bacteria were evaluated by the cross method, over plate test (OpT) and volatile organic compound test (VOCt). Bacteria with the best antibacterial potential were molecularly characterized, evaluated for antifungal activity and the presence of Iturin, Fengycin and Surfactin genes. Forty-six EBMM were isolated, most of them from the phylum Rhodophyta group and coming from Yacila. In the crossmatch evaluation, 10 EBMMs were found to inhibit gram-positive pathogenic bacteria (Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus and Listeria monocytogenes) and 12 gram-negative bacteria (Escherichia coli and Salmonella enteric sv typhimurium). These activities were confirmed by OpT and VOCt. The selected strains were identified as 4 Bacillus sp., 2 Kluyvera ascorbata , 1 Pantoea agglomerans , 1 Leclercia adecarboxylata and 1 Enterobacter sp. The strains, P. agglomerans YAFL6, Enterobacter sp. YAFL1, Bacillus sp. YCFR6 and Bacillus sp. YUFE8 showed high antifungal activity on Candida albicans , Candida tropicalis , Colletotrichium sp., Fusarium sp., Fusarium oxysporum and Alternaria sp. In addition, K. ascorbata YAFE21 and Bacillus sp. YCFE4 showed the presence of Iturin and Fengycin genes. The results obtained show the potential of the selected marine bacteria for future applications in the production of antibacterial metabolites and antifungal control. Bacteria fungi yeasts pathogens biocontrol infections Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 10 Aug, 2024 Read the published version in Archives of Microbiology → Version 1 posted Editorial decision: Revision requested 26 May, 2024 Reviewers agreed at journal 13 May, 2024 Reviews received at journal 09 May, 2024 Reviews received at journal 08 May, 2024 Reviewers agreed at journal 28 Apr, 2024 Reviewers agreed at journal 27 Apr, 2024 Reviewers invited by journal 27 Apr, 2024 Editor assigned by journal 26 Apr, 2024 Submission checks completed at journal 26 Apr, 2024 First submitted to journal 25 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4326823","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":296436018,"identity":"ec75736d-8932-49ed-90fc-8acde6ca8e3c","order_by":0,"name":"Edwin Jorge Vega 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EBMMs were isolated from \u003cem\u003eCaulerpa\u003c/em\u003e sp., \u003cem\u003eUlva\u003c/em\u003e sp., \u003cem\u003eAhnfeltiopsis\u003c/em\u003e sp. and \u003cem\u003eChondracantus chamissoi\u003c/em\u003e collected from Yacila and Cangrejo beach (Piura, Peru). Antimicrobial assays against pathogenic bacteria were evaluated by the cross method, over plate test (OpT) and volatile organic compound test (VOCt). Bacteria with the best antibacterial potential were molecularly characterized, evaluated for antifungal activity and the presence of Iturin, Fengycin and Surfactin genes. Forty-six EBMM were isolated, most of them from the phylum Rhodophyta group and coming from Yacila. In the crossmatch evaluation, 10 EBMMs were found to inhibit gram-positive pathogenic bacteria (Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus and Listeria monocytogenes) and 12 gram-negative bacteria (Escherichia coli and Salmonella enteric sv typhimurium). These activities were confirmed by OpT and VOCt. The selected strains were identified as 4 \u003cem\u003eBacillus\u003c/em\u003e sp., 2 \u003cem\u003eKluyvera ascorbata\u003c/em\u003e, 1 \u003cem\u003ePantoea agglomerans\u003c/em\u003e, 1 \u003cem\u003eLeclercia adecarboxylata\u003c/em\u003e and 1 \u003cem\u003eEnterobacter\u003c/em\u003e sp. The strains, \u003cem\u003eP. agglomerans\u003c/em\u003e YAFL6, \u003cem\u003eEnterobacter\u003c/em\u003e sp. YAFL1, \u003cem\u003eBacillus\u003c/em\u003e sp. YCFR6 and \u003cem\u003eBacillus\u003c/em\u003e sp. YUFE8 showed high antifungal activity on \u003cem\u003eCandida albicans\u003c/em\u003e, \u003cem\u003eCandida tropicalis\u003c/em\u003e, \u003cem\u003eColletotrichium\u003c/em\u003e sp., \u003cem\u003eFusarium\u003c/em\u003e sp., \u003cem\u003eFusarium oxysporum\u003c/em\u003e and \u003cem\u003eAlternaria\u003c/em\u003e sp. In addition, \u003cem\u003eK. ascorbata\u003c/em\u003e YAFE21 and \u003cem\u003eBacillus\u003c/em\u003e sp. 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