Comparison between two experimental methods of reproduction of subcutaneous endometriosis model in nude mice

Objective To compare the results of two experimental methods,namely subcutaneous transplant and subcutaneous injection,in reproducing endometriosis in nude mice,and study the differences in angiogenesis related factors.Methods Nude mice were divided two groups: in group A,the human endometrium fragments were transplanted into subcutaneously,and in group B the human endometrium suspension was subcutaneously injected.Each group was then divided into two subgroups according to inplantation time(2 and 4 weeks): group A1 and group A2,and group B1 and group B2.Vascular endothelial growth factor(VEGF) and microvessel density(MVD) were detected by SP method,and the weight and volume of ectopic focus between different groups were compared.Results At the same time points(2 week or 4 week),the volume of ectopic focus showed no significant difference between group A and group B(P0.05),but the weight of ectopic focus was significantly higher in group A than in group B(P0.05).There was no difference in VEGF expression and MVD in glandular cells and intercellular substance between group A and group B(P0.05),but the VEGF expression and MVD in gland cells were significantly higher in A1 and B1 groups at 2 weeks compared with that at 4-week point in A2 and B2 groups(P0.05).Conclusion It is successful in reproducing human endometriosis model in nude mice with both methods.Trasplantation method may be detter for observation of angiogenesis.