Trypsin-like proteinases and tissue inhibitors in adenomyosis and endometrial cancer.

e22215 Background: The activity of trypsin-like proteinases (ATP), kallikrein (K) and multipurpose inhibitors, plays an important part in development of neoplastic processes. The integrated study of parameters of tissue metabolism of endometrium in adenomyose (A) and endometrial cancer (EC) will allow supplementing the ideas about pathogenesis thereof. Methods: The tissue of endometrium of A (n=32, aged 49-59) and EC (n=46, aged 53-62, T2N0M0) was studied. The control was conventionally healthy endometrium – CHE. ATP, K, α-2M è α-1PI was determined in 10% cytosols using ethylic ether N-benzoyl-L-arginin as the substrate, and calculated in mkM/g for the crude tissue. Results: It has been determined that in A the total ATP was similar to the values in CHE, and it was 2 times as high in EC. The activity of K in A was 66.7% lower, while in EC the value was 1.3 times as high. The activity of α-2M and α-1PI was reduced in A 2.3 and 1.4 times, in EC – 9.1 and 1.2 times (r<0,05). ATP/K were higher in A and EC than in CHE, 1.6 and 1.5 times as high, respectively. It is essential that the percentages of active K in the total ATP in A and in EC were comparable, 17.2% and 18.4, respectively. So the activity of both inhibitors underwent unidirectional change. It is important to point out a significant excess (as compared to CHE level) of the Ê/α-2M ratio in EC. In A the parameter was higher than in CHE just 1.4 times, the ATP/α-1PI was higher in A and in EC – 1.3 and 2.3 times as high, respectively. Conclusions: Unidirectional change of the hydrolytic system components have been found in endometrial tissue in A and in EC that are manifested in significant reduction of activity of inhibitors and equal percentage of K in the total ATP. This allows considering them as an indicator of proliferative activity and expect similarity of proliferation mechanisms in both pathologic processes.