miR-577 suppressed the metastasis, EMT and viability via NF-κB pathway by targeting CXCL5 through in hepatocellular carcinoma

Abstract Background Hepatocellular carcinoma (HCC) is a common malignant cancer worldwide. miR-577 have a role in inhibiting cell viability, metastasis in many tumors. This research was to explore the great role of miR-577 in hepatocellular carcinoma. Methods RT-qPCR and western blot were performed to evaluate the the miR-577 and genes mRNA and protein levels. Transwell assay and CCK-8 were applied to measure the viable and invasive abilities. Meanwhile, Kaplan-Meier method was used to assess the survival of HCC patients. Results miR-577 was downregulated in HCC tissues, which predicted a worse overall survival in HCC. miR-577 targeted to CXCL5 and mediated its expression in HCC. miR-577 suppressed cell invasion and EMT in HuH-7 cells. miR-577 inhibited cell viability via NF-κB pathway. In addition, miR-577 overxpression impaired the xenograft growth of HuH-7 cells. Conclusion miR-577 inhibited cell invasion, EMT and viability via NF-κB pathway by targeting to CXCL5 in HCC. The newly identified miR-577/CXCL5 axis provides novel insight into the pathogenesis of hepatocellular carcinoma.