Identification and Vitro Validation of Biomarkers with Diagnostic and Prognostic for Lung Squamous Cell Carcinoma

Abstract Background: This study aimed to find biomarkers with diagnostic and prognostic for lung squamous cell carcinoma and to validate key biomarkers in vitroMethods：RNA sequencing was used to identify differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) between LUSC and normal tissue. Using published dataset, we also validated the result of RNA sequencing. The diagnostic and prognostic value of candidate genes were evaluated by ROC curve analysis and survival analysis, respectively. To uncover the confirm effect of MIR205HG in LUSC, we knocked down MIR205HG expression in NCI-H520 cells. Then, MTT assay and transwell assay was used to detect the effect of MIR205HG on cell proliferation and migration.Rsults: In total, 1946 DEmRNAs and 428 DElncRNAs were identified in LUSC compared to normal tissues. A total of 147 DElncRNAs-nearby target DEmRNAs pairs and 851 DElncRNA-DEmRNA co-expression pairs were obtained. Except for NEAT1, expression of the others in the The Cancer Genome Atlas (TCGA) results was consistent with our RNA sequencing, generally. ROC curve analysis displayed that the MCM2, SERPINB5, ITGB8, CASC19 and MIR205HG could predict the occurrence of LUSC. Survival analysis suggested that SERPINB5, NEAT1 and MIR205HG had potential prognostic value for LUSC. Knockdown of MIR205HG inhibits cell proliferation and migration in NCI-H520 cell. In addition, knockdown of MIR205HG significantly reduced the expression of ITGB8.Conclusions: This data may contribute to uncover the pathogenesis of LUSC and provide new and accurate therapeutic targets for LUSC.