Innovative non-pump gravity driving microfluid culture chip enhance mouse blastocyst formation

Innovative non-pump gravity driving microfluid culture chip enhance mouse blastocyst formation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Innovative non-pump gravity driving microfluid culture chip enhance mouse blastocyst formation Yao-Yuan Hsieh, Tsung-Tao Huang, Tzu-Chieh Kao, Chi-Chen Chang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4687604/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objectives: Various microfluidic technologies are currently used in ART. Herein we designed an innovative non-pump microfluidic culture platform to enhance the embryo culture in-vitro. We try to elucidate its feasibility and differences compared to traditional micro-drip culture systems. Materials and methods: We innovated a microfluidic chips with series procedures, including fabrication of an SU-8 master mould; casting of polydimethylsiloxane (PDMS) on mould; and integrating microfluidic device. All mouse embryos were divided: (1) traditional static drops; (2) microfluid static controls; (3) dynamic microfluid chip. We used the gravity plateform with tilt angle 15 degree to drive the culture medium through the whole microchannels. The cytotoxicity surveys of chip materials were performed with MTT assay. The embryo developments in each groups was compared. Results: The MTT surveys appeared the non-toxicity of PDMS chip materials. The percentages of 2 cell embryos reaching the stage of 4-8 cells, morula, and early blastocyst were comparable between both groups. In contrast, higher percentages of expanding blastocys and hatching blastocyst were observed in microfluid chip group. The percentages of 4-8 cells/morula/early/expanding/hatching blastocyst in each groups were 92.4/85.4/71.2/52.5/45.7%; 93.5/84.5/69.2/52.2/45.7%; and 93.8/86.5/74.5/67.2/60.4%, respectively. Conclusions: Combined microfluid and non-pump tilting culture system results in the dynamic stimulation of the embryos and automatic waste swept, which could not provided by traditional culture system. The tilting gravity instead of the motor driving system improves the convenience and feasibility for improving the embryo qualities of embryo culture in-vitro. Biological sciences/Biological techniques Biological sciences/Biotechnology culure chip microfluidics microchannel polydimethylsiloxane PDMS Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4687604","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":333153217,"identity":"2f387fb7-18fc-4386-9ad3-c4941af1bd82","order_by":0,"name":"Yao-Yuan Hsieh","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAy0lEQVRIiWNgGAWjYDCC8/2PHyRUMDAYEK/lxhk2gwdnkLTwENRyIIdB8mEbKVr4Dpw9YJA477C8OXvzAYYfFdsY7CUS8GuRPNyX8CBx22HDnT3HEhh7ztxm4CGkxeDAAQMDoBbGDTdyDJgZ24BapAlqSTCQSJxz2J4ULTlALQ2HE4nXInnjWJpBwrH05A1njiUcBPqFh+f+A/xa+M43H374o8badsPx5oMPflTclmPvOYBfCxQ0g0mQWsIxCQV1xCocBaNgFIyCkQgAxEtOj80r7eAAAAAASUVORK5CYII=","orcid":"","institution":"Hsieh Women Clinics","correspondingAuthor":true,"prefix":"","firstName":"Yao-Yuan","middleName":"","lastName":"Hsieh","suffix":""},{"id":333153218,"identity":"a7859b9c-bb3c-4d75-8734-0fb6cf62dcfa","order_by":1,"name":"Tsung-Tao Huang","email":"","orcid":"","institution":"Taiwan Instrument Research Institute, National Applied Research Laboratories","correspondingAuthor":false,"prefix":"","firstName":"Tsung-Tao","middleName":"","lastName":"Huang","suffix":""},{"id":333153219,"identity":"94dca682-1ec7-4686-90a7-2db8d8ce0791","order_by":2,"name":"Tzu-Chieh Kao","email":"","orcid":"","institution":"Jamon Incorporation","correspondingAuthor":false,"prefix":"","firstName":"Tzu-Chieh","middleName":"","lastName":"Kao","suffix":""},{"id":333153220,"identity":"1769f4a2-01f3-4de9-b7b2-619d710cde64","order_by":3,"name":"Chi-Chen Chang","email":"","orcid":"","institution":"Hsieh Women Clinics","correspondingAuthor":false,"prefix":"","firstName":"Chi-Chen","middleName":"","lastName":"Chang","suffix":""},{"id":333153221,"identity":"59ae1deb-8b5e-4ac0-b88c-93d0fe9e25f7","order_by":4,"name":"Jen-Hsiang Hsieh","email":"","orcid":"","institution":"Taichung Veterans General Hospital","correspondingAuthor":false,"prefix":"","firstName":"Jen-Hsiang","middleName":"","lastName":"Hsieh","suffix":""}],"badges":[],"createdAt":"2024-07-04 15:33:59","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4687604/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4687604/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":64269461,"identity":"f53f0593-e438-49d8-8695-79f6bf6a03e9","added_by":"auto","created_at":"2024-09-11 05:05:03","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":552496,"visible":true,"origin":"","legend":"","description":"","filename":"ScientificReport.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4687604/v1_covered_83e6c32e-b4e0-4c57-9055-a7f2445bb118.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Innovative non-pump gravity driving microfluid culture chip enhance mouse blastocyst formation","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"culure chip, microfluidics, microchannel, polydimethylsiloxane, PDMS","lastPublishedDoi":"10.21203/rs.3.rs-4687604/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4687604/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjectives:\u003c/strong\u003e Various microfluidic technologies are currently used in ART. Herein we designed an innovative non-pump microfluidic culture platform to enhance the embryo culture in-vitro. We try to elucidate its feasibility and differences compared to traditional micro-drip culture systems.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials and methods: \u003c/strong\u003eWe innovated a microfluidic chips with series procedures, including fabrication of an SU-8 master mould; casting of polydimethylsiloxane (PDMS) on mould; and integrating microfluidic device. All mouse embryos were divided: (1) traditional static drops; (2) microfluid static controls; (3) dynamic microfluid chip. We used the gravity plateform with tilt angle 15 degree to drive the culture medium through the whole microchannels. The cytotoxicity surveys of chip materials were performed with MTT assay.\u003cstrong\u003e \u003c/strong\u003eThe embryo developments in each groups was compared.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003eThe MTT surveys appeared the non-toxicity of PDMS chip materials. The percentages of 2 cell embryos reaching the stage of 4-8 cells, morula, and early blastocyst were comparable between both groups. In contrast, higher percentages of expanding blastocys and hatching blastocyst were observed in microfluid chip group. The percentages of 4-8 cells/morula/early/expanding/hatching blastocyst in each groups were 92.4/85.4/71.2/52.5/45.7%; 93.5/84.5/69.2/52.2/45.7%; and 93.8/86.5/74.5/67.2/60.4%, respectively.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusions: \u003c/strong\u003eCombined microfluid and non-pump tilting culture system results in the dynamic stimulation of the embryos and automatic waste swept, which could not provided by traditional culture system. The tilting gravity instead of the motor driving system improves the convenience and feasibility for improving the embryo qualities of embryo culture in-vitro.\u003c/p\u003e","manuscriptTitle":"Innovative non-pump gravity driving microfluid culture chip enhance mouse blastocyst formation","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-30 04:51:00","doi":"10.21203/rs.3.rs-4687604/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d5ba121c-3c3c-4506-a147-f9e693a4be7f","owner":[],"postedDate":"July 30th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":35273038,"name":"Biological sciences/Biological techniques"},{"id":35273039,"name":"Biological sciences/Biotechnology"}],"tags":[],"updatedAt":"2024-09-11T04:56:56+00:00","versionOfRecord":[],"versionCreatedAt":"2024-07-30 04:51:00","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4687604","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4687604","identity":"rs-4687604","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}