Bessel beam tomography for fast volume imaging

Light microscopy on dynamic samples, for example neural activity in the brain, requires imaging large volumes at high rates. Here, we develop a tomography approach for scanning fluorescence microscopy which allows recording volume images at frame scan rates. Volumes are imaged by simultaneously recording four independent projections at different angles using temporally multiplexed, tilted Bessel beams. From the resulting projections, volumes are reconstructed using inverse Radon transforms combined with three dimensional convolutional neural networks (U-net). This tomography approach is suitable for experiments requiring fast volume imaging of sparse samples, as for example often encountered when imaging neural activity in the brain.