Histological Evaluation of Uterus and Bone response to Hormonal Contraceptive in Rats

Mohammed F. Ali, Isam Hamo Mahmood, Tahani A. Azeez
In: Research Journal of Pharmacy and Technology · 2023 · pp. 686–690 · doi:10.52711/0974-360x.2023.00117 · W4362587596
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This study histologically evaluated the effects of oral and injectable hormonal contraceptives on the uterus and bone in rats, finding significant differences in articular cartilage, metaphyseal plate, trabecular bone, and endometrium thickness compared to controls.

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The study evaluated histological effects of oral versus injectable hormonal contraceptives on uterine tissues (including endometrium, cervix, and ovary) and on bone-related structures in 30 female albino rats, compared with a control group, after 8 weeks of daily oral dosing or weekly injectable dosing. Compared with controls, the experimental groups showed significant differences in articular cartilage thickness, metaphyseal plate thickness, trabecular bone thickness, and endometrial thickness, indicating histological changes in both uterus and bone tissues. The authors acknowledge a key limitation in that the work is limited to histological endpoints in an animal model over a fixed 8-week period without broader functional or mechanistic assessments. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Background: Sex hormones play an important role in uterus and bone tissue integration. Oral hormonal contraceptive pills (OCPs) were developed over 50 years ago. Aims of the study: to clarify the histological evaluation of different types of hormonal contraceptive effects on uterus, cervix, ovary, endometrium, and bone in the female albino rat. Materials and methods: The study was conducted on thirty albino rats. three groups each group 10 rats one of them considered as a control group and the other two groups considered as a treated group which taking oral hormonal contraceptive once daily for about 8 weeks and another group takes injectable hormonal contraceptive once weekly for about 8 weeks then euthanized for histological examination. Result: After 8 weeks of drug administration histological findings show significant differences in the thickness of articular cartilage, the thickness of metaphyseal plate, the thickness of trabecular bone, and thickness of endometrium between the control group and experimental group. Conclusion: The current study showed that hormonal contraceptives exhibited histological changes on the uterus and bone which can be correlated to different clinical findings.
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Abstract

Background: Sex hormones play an important role in uterus and bone tissue integration. Oral hormonal contraceptive pills (OCPs) were developed over 50 years ago. Aims of the study: to clarify the histological evaluation of different types of hormonal contraceptive effects on uterus, cervix, ovary, endometrium, and bone in the female albino rat. Materials and methods: The study was conducted on thirty albino rats. three groups each group 10 rats one of them considered as a control group and the other two groups considered as a treated group which taking oral hormonal contraceptive once daily for about 8 weeks and another group takes injectable hormonal contraceptive once weekly for about 8 weeks then euthanized for histological examination. Result: After 8 weeks of drug administration histological findings show significant differences in the thickness of articular cartilage, the thickness of metaphyseal plate, the thickness of trabecular bone, and thickness of endometrium between the control group and experimental group. Conclusion: The current study showed that hormonal contraceptives exhibited histological changes on the uterus and bone which can be correlated to different clinical findings. Cite this article: Mohammed F. Ali, Isam H. Mahmood, Tahani A. Azeez. Histological Evaluation of Uterus and Bone response to Hormonal Contraceptive in Rats. Research Journal of Pharmacy and Technology 2023; 16(2):686-0. doi: 10.52711/0974-360X.2023.00117 Cite(Electronic): Mohammed F. Ali, Isam H. Mahmood, Tahani A. Azeez. Histological Evaluation of Uterus and Bone response to Hormonal Contraceptive in Rats. Research Journal of Pharmacy and Technology 2023; 16(2):686-0. doi: 10.52711/0974-360X.2023.00117 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-2-36

References

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