Abstract
The genus Xylaria is a promising source of bioactive compounds. This study explored the diversity of secondary metabolites (SMs) in Xylaria longipes under three fermentation conditions, isolating 14 new SMs and 27 known ones. The structures were elucidated using NMR, HRESIMS, ROESY, and J-based configuration analysis, with absolute configurations determined through calculated ECD and 13 C NMR data. A comprehensive library of SMs was constructed, facilitating metabolomics-wide association studies that identified culture conditions as a critical factor influencing SM production. This compound library also simplifies the determination of absolute configurations for diplosporins by analyzing J values and CD trends. Anti-proliferative assays against induced T/B lymphocytes and HaCaT cells revealed that more than half of the compounds exhibited significant inhibitory activity, with compounds 2 , 15 , and 32 reducing IFN-γ secretion. Compound 32 showed promising anti-psoriatic effects by inhibiting NF-κB p65 phosphorylation in HaCaT cells. This first systematic chemical study of X. longipes under varied conditions provides insights into structure-activity relationships.
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ARPHA Preprints
https://doi.org/10.3897/arphapreprints.e156971 (29 Apr 2025)
https://doi.org/10.3897/arphapreprints.e156971 (29 Apr 2025)
Published in: IMA Fungus https://doi.org/10.3897/imafungus.16.153522
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ARPHA Preprints
doi:
10.3897/arphapreprints.e156971
First posted
29 Apr 2025
Authors
Zhen-Zhu Zhao
- Corresponding author
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Henan University of Chinese Medicine, Zhengzhou, China
Xin Pang
- Corresponding author
Henan University of Chinese Medicine, Zhengzhou, China
Wei-Sheng Feng
- Corresponding author
Henan University of Chinese Medicine, Zhengzhou, China
Conflict of interest
The authors have declared that no competing interests exist.
Disclaimer: This article is (co-)authored by any of the Editors-in-Chief, Managing Editors or their deputies in this journal.
This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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