Abstracts of the Annual Meeting of the Iuliu Haţieganu, University of Medicine and Pharmacy : 3rd – 7th December 2018, Cluj-Napoca

Sevastre - Berghian A, Hanganu D, Filip G, Mitrea D, Szel M, Hoteiuc O, Bidian C, Olteanu D, Clichici S, David L, Moldovan B, Filip A, Ujvary P, Necula V, Maniu A, Gheban D, Uifălean A, Boarescu P, Chirilă I, Cioca L, Bulboacă A, Capraş R, Login C, Baldea I, Tiperciuc B, Decea N, Suciu S, Bocşan I, Muntean I, Neag M, Deleanu D, Crăciun C, Crăciun A, Bocşan C, Brumboiu M, Alsharoh H, Hurdiel R, Peze T, Zunquin G, Porrovecchio A, Mogosan C, Calinici T, Tavolacci M, Ladner J, Zubaşcu P, Goadă D, Puscas E, Stoeber L, Parvu M, Uifalean A, Ţicolea M, Fărcaş A, Moţ A, Kulak M, Pârvu A, Dudea-Simon M, Schiau C, Dudea S, Feier D, Mocan O, Rădulescu D, Coman A, Cristea A, Pop R, Puia I, Puia A, Chedea V, Boca A, Seillier M, Vanheesbeke M, Lambinet M, Sekkal M, Jurcău R, Jurcău I, Colceriu N, Ilieş R, Cătană A, Popp R, Schiotis R, Rednic S, Buzoianu A, Tompa M, Ciontea M, Pandrea S, Tompa R, Pepelea L, Matroş L, Oniţiu N, Ţaranu I, Drugan C, Cătană C, Drugan T, Chis A, Bunea I, Lazea C, Oprita S, Abrudan G, Buzdugan E, Grosu A, Crisan D, Radulescu D, Dudric V, Constantea N, Avram L, Miclaus D, Chiorescu R, Barta A, Mare C, Jianu C, Filipescu I, Topan A, Itu-Mureşan C, Pop S, Mansour M, Blendea D, McPherson C, Stoia M, Mocan M, Anton F, Chevalier J, Singh J, Andreica S, Burac L, Pacurar D, Bodea L, Militaru M, Maris A, Sirbe C, Gheorhe P, Asavoaie C, Grigore M, Grama A, Cosarca M, Pop T, Zaharie G, Blaga L, Matyas M, Lamy E, Hasmasanu M, Morariu D, Goron A, Roman G, Leach N, Răcătăianu N, Para I, Sâmpelean D, Nechifor O, Török T, Racz O, Emma F, Konrad M, Fufezan O, Bulata B, Bungardi A, Cainap S, Pop V, Zsoldos A, Stanciu S, Bojan A, Parvu A, Asafti A, Mako L, Roşu P, Isip O, Mureşanu D, Stoia P, Stache-Ciucanu O, Vulcu S, Vlaicu I, Bălăcescu L, Lisencu C, Puşcaş E, Burz C, Faur D, Răchişan A, Tomşa A, Chezan C, Borzan C, Cherecheş-Panţa P, Dudilă S, Puşcaş L, Rachisan A, Dubois V, Ranchin B, Leclerc A, Thomas A, Cochat P, Bacchetta J, Vulturar R, Dumitru-Ionescu G, Miu A, Coste S, Breaban I, Stefan A, Popovici I, Taut A, Sarlea S, Cozma A, Orasan O, Sampelean D, Negrean V, Procopciuc L, Kalesis N, Vlad C, Rajnoveanu R, Gergely-Domokos B, Gluhovschi A, Motoc N, Man M, Magdau C, Leşan A, Todea D, Chiş A, Aioanei C, Damian I, Nicoara S, Malutan A, Clinciu D, Mirescu S, Ciortea R, Mocan-Hognogi R, Dudea M, Mihu D, Pop M, Fiţ A, Bartoş D, Vesa Ş, Puia C, Nadim A, Iancu C, Nechita V, Leucuţa D, Bolboacă S, Dragotă M, Cosma A, Al-Hajjar N, Graur F, Oltean-Dan D, Dogaru G, Apostu D, Mester A, Benea H, Paiusan M, Dan C, Bardas C, Popa C, Jianu E, Toader A, Tomoaia G, Ujváry L, Sevastre B, Potârniche A, Blebea C, Dindelegan M, Chirilă M, Chifiriuc M, Popa M, Coman F, Mbaveng A, Leonte D, Bencze L, Imre S, Kuete V, Efferth T, Zaharia V, Iacoviţă C, Topală T, Dudric R, Scoruş L, Florea A, Tetean R, Lucaciu C, Gliga L, Oprean L, Hosu O, Selvolini G, Băjan I, Marrazza G, Cernat A, Györfi S, Irimes M, Bodoki A, Suciu M, Melinte G, Şimon I, Ştiufiuc R, Săndulescu R, Nagy K, Palmieri C, Duez P, Oniga S, Araniciu C, Marc G, Stoica C, Palage M, Uncu L, Oniga O, Stoian A, Iacob B, Bodoki E, Prates Ramalho J, Oprean R, Vedeanu N, Voica C, Magdas A, Ştefan M, Simedrea R, Georgiu C, Voştinaru O, Grozav A, Diuzheva A, Bădărău S, Carradori S, Locatelli M, Soković M, Barbălată C, Porfire A, Boşca A, Farkas A, Cazacu I, Apan B, Safta D, Somotecan A, Brumboiu I, Pop C, Berce C, Ghibu S, Scurtu I, Khuroo A, Kochhar R, Oroian M, Curean T, Marcovici A, Bhardwaj S, Epure A, Oniga I, Colobăţiu L, Gâvan A, Mirel S, Hurubă M, Farcaş-Călugăr A, Ilyés K, Kovács N, Balogh A, Borbás E, Farkas B, Casian T, Marosi G, Nagy Z, Fizeşan I, Cambier S, Moschini E, Chary A, Nelissen I, Ziebel J, Audinot J, Wirtz T, Kruszewski M, Kiss B, Serchi T, Loghin F, Gutleb A, Abrudan M, Muntean D, Şipoş M, Farcaş A, Mogoşan C, Benedec D, Bogdan C, Iurian S, ROMAN C, Rusu M, Mocan A, Gheldiu A, Moldovan C, Tomuţă I, Ielciu I, Angenot L, Păltinean R, Ledoux A, Vlase L, Crişan G, Frédérich M, Crişan M, Lenghel M, Manea A, Comsa D, Pop D, Buiga O, Vacaras S, Armencea G, Dinu C, Buhăţel D, Sarbu A, Mirică C, Ciui B, Tertiş M, Cristea C, Cosma L, Suhani R, Mesaros A, Badea M, Vlas I, Aghiorghiesei A, Manziuc M, Lazar (Moldovan) M, Rotar A, Baciut G, Prejmerean C, Prodan D, Balc N, Bere P, Aluaş M, Bordea R, Buduru S, Zaharia A, Popa D, Bocsan C, Mirica I, Campian R, Petrescu N, Ilea A, Lucaciu O, Băbţan A, Boşca B, Feurdean C, Buhatel D, Ionel A, Mirica C, Sava A, Pop A, Uriciuc W, Câmpian R, Moga R, Chiorean C, Iacob S, Fluerasu M, Berar A, Lascu L, Chisnoiu R, Rotaru D, Păstrav O, Hrab D, Chisnoiu A, Saroşi C, Moldovan M, Bunta O, Ţărmure V, Băciuţ M, Florea D, Delean A, Mocanu A, Tomoaia-Cotisel M, Greta D, Gasparik C, Colosi H, Dudea D, Simon I, Hedesiu M, Virag P, Salmon B, Tarmure V, Baciut M, Bran S, Jacobs R, Falamas A
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Wild and cultured Thymus Marshallianus extracts improved antioxidant defense, reduced blood glucose, and alleviated anxiety-like behaviors in diabetic rats.

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This study evaluated the comparative effects of wild and cultured Thymus Marshallianus extracts on oxidative stress and behavioral changes in a diabetic rat model induced by streptozotocin. The researchers conducted in vitro experiments on human umbilical vein endothelial cells and in vivo trials using Wistar rats to assess biomarkers such as MDA, NF-kB, and HDAC1 alongside anxiety-like behaviors measured by open field and elevated plus maze tests. Results indicated that both extracts reduced blood glucose levels, enhanced antioxidant capacity in the frontal lobe, and significantly improved anxiety-like behavior by increasing time spent in open arms. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Intro

Diabetes mellitus (DM) is a chronic metabolic disorder that can associate with oxidative stress and behavioral changes. We aimed to evaluate the comparative effects of two species of Thymus Marshallianus (TM), wild flora (TMW) and culture (TMC) on both in vitro and in vivo models.

Results

In vitro, TMW and TMC diminished MDA, NF-kB and γH2AX levels and increased pNF-kB and HIF 1α expressions. In vivo, TMW and TMC administration reduced blood glucose levels, improved the overall mobility and increased 5 times the entrances and time spent in the open arms in EPM. In frontal lobe, both extract diminished lipid peroxidation and enhanced the antioxidant capacity and HDAC1 expression. TMW administration increased NF-kB level and diminished MECP2 expression in hippocampus.

Conclusions

Both compounds exerted beneficial effect by increasing the antioxidant defense and improving the anxiety-like behavior.

Materials|Methods

In vitro studies were performed on HUVEC cell line, using TMW and TMC in two different dilutions (1/10000, 1/100000), under normoglycemic and hyperglycemic conditions. NF-kB, activated pNF-kB, HIF 1α and γH2AX were assessed by western blot and MDA levels by spectrofluorimetry. 36 Wistar rats were used for the in vivo study. The animals were divided in 4 groups (n=9/group): Control (Carboxymethylcellulose, CMC), DM, DM + TMW, DM+ TMC. TMW and TMC (200 mg/kg b.w.) were orally administered for 14 days. On the 15th day, one dose of STZ (30 mg/kg b.w.) was intraperitoneally administered. Subsequently, natural compounds were administrated for the next 14 days. On the 33rd day, Open Field Test and Elevated Plus Maze were performed. Oxidative stress biomarkers in serum, hippocampus and frontal lobe homogenates (MDA, GSH/GSSG) and NF-kB levels in hippocampus and frontal lobe samples were also assessed. Methyl CpG binding protein (MECP) 2 and histone deacetylase 1 (HDAC1) expressions in rats’ brain were also analyzed by western blot.

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