Mitochondrial Dynamics of Bcl-2 Family During E2-Induced Apoptosis Correlates with the Malignant of Endometrial Cancer Cells

Mitochondrial Dynamics of Bcl-2 Family During E2-Induced Apoptosis Correlates with the Malignant of Endometrial Cancer Cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Mitochondrial Dynamics of Bcl-2 Family During E 2 -Induced Apoptosis Correlates with the Malignant of Endometrial Cancer Cells Takahiro Yaguchi, Hirofumi Taira, Misaki Kameno, Junji Kawakami This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1260502/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Endometrial adenocarcinoma has two type: Type I has an estrogen-derived etiology and low-grade endometrial cancer cells, on the other hand, Type II occurs estrogenindependent and is high-grade endometrial cancer cells. However, it is not well understood that the relationship between the exposure of estrogen and the grade of endometrial cancer cells. So, we investigated how estrogen affected the grade of endometrial cancer cells. In the present study, we focused on the localization and expression of Bcl-2 family that regulate the mitochondrial membrane potential in HEC1 and HEC50B cells. Cell viability was decreased by 17-b-estradiol (E 2 ) and E 2 disturbed the mitochondrial membrane potential followed by activating caspase-9 and caspase-3 in both cells. These results suggested that E 2 induced apoptosis in HEC1 and HEC50B cells. B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated death promoter (Bad) expression were decreased and B-cell lymphoma-extra large (Bcl-XL ) and Bcl-2-associated X protein (Bax) expression were increased at the mitochondrial outer membrane in HEC1 cells. On the other hand, Bcl-2 and Bcl-X L expression were decreased and Bad and Bax expression were increased at the mitochondrial outer membrane in HEC50B cells. In conclusion, the dynamics of the Bcl-2 family during E2 -induced apoptosis was found to be different depending on the grade of endometrial cancer cells. We hope that the dynamics of Bcl-2 family proteins such as Bcl-X L and Bad will be used to diagnose the grade of malignant of endometrial cancer cells. E2 mitochondrial membrane potential Bcl-2 family caspase apoptosis Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Full Text Tables Table 1 is only available as a download in the Supplemental Files section. Supplementary Files Table1Yaguchi.pdf Table 1 Primers used for RT-PCR. SupplementalTable1Yaguchi.pdf Supplementary Table 1 Primers used for RT-PCR. supplyfig1Yaguchi.tif Supplementary Fig. 1 RT-PCR analysis is performed for p53 mRNA expressed in HEC1 cells (a: upper panel) and HEC50B cells (b: lower panel). Cells were treated with E2 (50 µM) for 0, 0.5, 1, 3 and 6 h and RNA was extracted followed by RT-PCR. Note that a similar result was obtained with 3 independent experiments. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1260502","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":79990198,"identity":"71f8a757-ccef-49de-906b-fdbb3e3882a8","order_by":0,"name":"Takahiro Yaguchi","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzUlEQVRIiWNgGAWjYLACxgYJOTYGHhiHSC3GJGthSGyAaSEIDI4fvybxc4dFep9E7jEJhho7BubZBKwxOJNTJtl7RiK3TSIvTYLhWDID45wDBLQcyEmTZmwDackxu8HAdoCBcUYCAS3n34C1pLOBtfwjRsuN9GMgLQlgLYxtRGiRvPGG2bK3TcKwjeeN+Y/EvmQegn7hO5/+8MbPtjp5+fYcY4MP3+zkDAmFmMIBHgMED+gkHsMZ+HUwyDewP0ATkSCgZRSMglEwCkYcAACMVUFxA29+zQAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0002-4660-5123","institution":"International University of Health and Welfare Department of Healthcare: Kokusai Iryo Fukushi Daigaku Fukuoka Hoken Gakubu","correspondingAuthor":true,"prefix":"","firstName":"Takahiro","middleName":"","lastName":"Yaguchi","suffix":""},{"id":79990199,"identity":"b7f0f5d1-6b20-4f31-8e9a-cec920fce69d","order_by":1,"name":"Hirofumi Taira","email":"","orcid":"","institution":"International University of Health and Welfare: Kokusai Iryo Fukushi Daigaku","correspondingAuthor":false,"prefix":"","firstName":"Hirofumi","middleName":"","lastName":"Taira","suffix":""},{"id":79990200,"identity":"23907385-6e23-4fad-86cf-8a7eda58db48","order_by":2,"name":"Misaki Kameno","email":"","orcid":"","institution":"Konan University: Konan Daigaku","correspondingAuthor":false,"prefix":"","firstName":"Misaki","middleName":"","lastName":"Kameno","suffix":""},{"id":79990201,"identity":"eac8f934-0bb8-4852-b8fa-95945ca6e74e","order_by":3,"name":"Junji Kawakami","email":"","orcid":"","institution":"Konan University: Konan Daigaku","correspondingAuthor":false,"prefix":"","firstName":"Junji","middleName":"","lastName":"Kawakami","suffix":""}],"badges":[],"createdAt":"2022-01-14 11:18:01","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1260502/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1260502/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":17823316,"identity":"39464964-91a1-49f1-b776-eecacbc38104","added_by":"auto","created_at":"2022-01-31 19:52:33","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":121516,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e attenuates cell viability in HEC1 cells and HE50B cells. (a) HEC1 cells and (b) HEC50B cells were treated with E\u003csub\u003e2 \u003c/sub\u003eat concentration as indicated for 24 h and 48 h. Cell viability for 5,000 cells per well (96 well) was measured using an MTT assay. In the graphs, each point represents the mean ± SEM ratio of basal level (n=5-7 independent experiments). **\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01; *\u003cem\u003eP\u003c/em\u003e\u0026lt;0.05, ††\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01; †\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01, P values, unpaired \u003cem\u003et\u003c/em\u003e-test.\u003c/p\u003e","description":"","filename":"figure1Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/95a937af2854c26237378e78.png"},{"id":17823319,"identity":"d0e376d0-d2e4-4d0a-bb19-982131e25de3","added_by":"auto","created_at":"2022-01-31 19:52:33","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":804124,"visible":true,"origin":"","legend":"\u003cp\u003eRT-PCR analysis is performed for ERα, ERβ, ERRα, ERRβ, ERRγ and GAPDH mRNA expressed in HEC1 cells (a: upper panel) and HEC50B cells (a: lower panel). HEC1 cells (b: upper panels) and HEC50B cells (b: lower panels) were treated with E2 (50 µM) for 0, 0.5, 1, 3 and 6 h and RNA was extracted followed by RT-PCR. Note that a similar result was obtained with 3 independent experiments.\u003c/p\u003e","description":"","filename":"figure2Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/4de086dd916a117384c0ade3.png"},{"id":17823477,"identity":"0782761d-f993-498e-947d-912b5d5f234a","added_by":"auto","created_at":"2022-01-31 19:55:34","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":640853,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e (50 µM) disturbs mitochondrial membrane potentials in HEC1 cells sand HEC50B cells. HEC1 cells (a) and HEC50B cells (b) were treated with E\u003csub\u003e2\u003c/sub\u003e (50 µM) for 12 h in serum free culture medium, and mitochondrial membrane potentials were observed with BZ-X810. The aggregate red form (a: left 2 panels, b: left 2 panels) was detected at an absorption/emission of 585/590 nm and the green monomeric form (a: right 2 panels, b: right 2 panels) was detected at an absorption/emission of 510/527 nm. Scale bars, 50 µm. Note that a similar result was obtained with 4-5 independent experiments\u003c/p\u003e","description":"","filename":"figure3Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/dad137e585fca25b38ad25ec.png"},{"id":17823324,"identity":"47e6d58a-f089-4b9a-8274-5f35f20cc780","added_by":"auto","created_at":"2022-01-31 19:52:34","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":228963,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e (50 µM) changes the expression and localization of Bcl-2 family and Cyt c in HEC1 cells. After 6-h treatment with E2 (50 µM), Western blotting for Bcl-X\u003csub\u003eL\u003c/sub\u003e (a), Bcl-2 (b), Bad (c), Bax (d), Cyt c (e) and β-actin (f) was performed in the mitochondrial and cytoplasmic fractions. In the graph, each column represents the mean ± SEM ratio against basal normalized immunoreactive intensities for mitochondrial and cytoplasmic fractions untreated with E2 (50 µM) (n=4-6 independent experiments). **\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01; *\u003cem\u003eP\u003c/em\u003e\u0026lt;0.05, P values, unpaired \u003cem\u003et\u003c/em\u003e-test.\u003c/p\u003e","description":"","filename":"figure4Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/cd4264f32015a782fa7c293e.png"},{"id":17823771,"identity":"65ee579d-3c82-49d3-adce-adb293aead6a","added_by":"auto","created_at":"2022-01-31 19:58:34","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":258533,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e (50 µM) changes the expression and localization of Bcl-2 family and Cyt c in HEC50B cells. After 6-h treatment with E2 (50 µM), Western blotting for Bcl-XL (a), Bcl-2 (b), Bad (c), Bax (d), Cyt c (e) and β-actin (f) was performed in the mitochondrial and cytoplasmic fractions. In the graph, each column represents the mean ± SEM ratio against basal normalized immunoreactive intensities for mitochondrial and cytoplasmic fractions untreated with E2 (50 µM) (n=4-6 independent experiments). **\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01; *\u003cem\u003eP\u003c/em\u003e\u0026lt;0.05, \u003cem\u003eP\u003c/em\u003e values, unpaired \u003cem\u003et\u003c/em\u003e-test.\u003c/p\u003e","description":"","filename":"figure5Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/19953172401cfb4a78d5529f.png"},{"id":17823480,"identity":"4938ca43-10e2-40d4-9429-6798ab8edfe5","added_by":"auto","created_at":"2022-01-31 19:55:34","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":103108,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e (50 µM) activates caspase-9 and caspase-3 in HEC1 cells. After 12-h treatment with E2 (50 µM), activities of caspase-9 (a) and -3 (b) were measured with spectrofluorometer (RF-5300). Note that a similar result was obtained with 5 independent experiments. **\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01, \u003cem\u003eP\u003c/em\u003e values, unpaired \u003cem\u003et\u003c/em\u003e-test.\u0026nbsp;\u003c/p\u003e","description":"","filename":"figure6Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/ed4a8d02d0bf9fb641a49110.png"},{"id":17823478,"identity":"b08c55d9-61c4-4b1d-8c48-5d2a4dc72afb","added_by":"auto","created_at":"2022-01-31 19:55:34","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":90028,"visible":true,"origin":"","legend":"\u003cp\u003eE\u003csub\u003e2\u003c/sub\u003e (50 µM) activates caspase-9 and caspase-3 in HEC50B cells. After 12-h treatment with E\u003csub\u003e2\u003c/sub\u003e (50 µM), activities of caspase-9 (a) and -3 (b) were measured with spectrofluorometer (RF-5300). Note that a similar result was obtained with 4-5 independent experiments. **\u003cem\u003eP\u003c/em\u003e\u0026lt;0.01; *\u003cem\u003eP\u003c/em\u003e\u0026lt;0.05, \u003cem\u003eP\u003c/em\u003e values, unpaired \u003cem\u003et\u003c/em\u003e-test.\u003c/p\u003e","description":"","filename":"figure7Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/aabefb9dbeba3e05d6f8ee49.png"},{"id":17823318,"identity":"e2713cf0-cb15-4f27-a683-ea97fa898f9b","added_by":"auto","created_at":"2022-01-31 19:52:33","extension":"png","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":114915,"visible":true,"origin":"","legend":"\u003cp\u003eSchematic diagram for E\u003csub\u003e2\u003c/sub\u003e signaling pathways.\u003c/p\u003e","description":"","filename":"Figure8Yaguchi.png","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/7d18d69c1624d75246d19904.png"},{"id":17823772,"identity":"5907ba4a-9048-424d-a0d5-823f426462d6","added_by":"auto","created_at":"2022-01-31 19:58:39","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":324043,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptYaguchi.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1_covered.pdf"},{"id":17823321,"identity":"5cd07f21-8cf8-443f-956c-70dc89b71511","added_by":"auto","created_at":"2022-01-31 19:52:34","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":35135,"visible":true,"origin":"","legend":"\u003cp\u003eTable\u0026nbsp;1 Primers used for RT-PCR.\u003c/p\u003e","description":"","filename":"Table1Yaguchi.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/889a5769644d9a2a741e90f4.pdf"},{"id":17823317,"identity":"5c465cb1-9bb1-4fdb-9b83-18ad8d4d8935","added_by":"auto","created_at":"2022-01-31 19:52:33","extension":"pdf","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":27708,"visible":true,"origin":"","legend":"\u003cp\u003eSupplementary Table 1 Primers used for RT-PCR.\u003c/p\u003e","description":"","filename":"SupplementalTable1Yaguchi.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/735edf9c7cfd8afae312b760.pdf"},{"id":17823326,"identity":"5e5b4162-7661-4e88-9978-01b70eeb87a9","added_by":"auto","created_at":"2022-01-31 19:52:34","extension":"tif","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":13996744,"visible":true,"origin":"","legend":"\u003cp\u003eSupplementary Fig. 1 RT-PCR analysis is performed for p53 mRNA expressed in HEC1 cells (a: upper panel) and HEC50B cells (b: lower panel). Cells were treated with E2 (50 µM) for 0, 0.5, 1, 3 and 6 h and RNA was extracted followed by RT-PCR. Note that a similar result was obtained with 3 independent experiments.\u003c/p\u003e","description":"","filename":"supplyfig1Yaguchi.tif","url":"https://assets-eu.researchsquare.com/files/rs-1260502/v1/19a7fadd32225ca31bddabf0.tif"}],"financialInterests":"","formattedTitle":"\u003cp\u003eMitochondrial Dynamics of Bcl-2 Family During E\u003csub\u003e2\u003c/sub\u003e-Induced Apoptosis Correlates with the Malignant of Endometrial Cancer Cells\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1260502/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."},{"header":"Tables","content":"\u003cp\u003eTable 1 is only available as a download in the Supplemental Files section.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"E2, mitochondrial membrane potential, Bcl-2 family, caspase, apoptosis","lastPublishedDoi":"10.21203/rs.3.rs-1260502/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1260502/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eEndometrial adenocarcinoma has two type: Type I has an estrogen-derived etiology and low-grade endometrial cancer cells, on the other hand, Type II occurs estrogenindependent and is high-grade endometrial cancer cells. However, it is not well understood that the relationship between the exposure of estrogen and the grade of endometrial cancer cells. So, we investigated how estrogen affected the grade of endometrial cancer cells. In the present study, we focused on the localization and expression of Bcl-2 family that regulate the mitochondrial membrane potential in HEC1 and HEC50B cells. Cell viability was decreased by 17-b-estradiol (E\u003csub\u003e2\u003c/sub\u003e ) and E\u003csub\u003e2\u003c/sub\u003e disturbed the mitochondrial membrane potential followed by activating caspase-9 and caspase-3 in both cells. These results suggested that E\u003csub\u003e2\u003c/sub\u003e induced apoptosis in HEC1 and HEC50B cells. B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated death promoter (Bad) expression were decreased and B-cell lymphoma-extra large (Bcl-XL ) and Bcl-2-associated X protein (Bax) expression were increased at the mitochondrial outer membrane in HEC1 cells. On the other hand, Bcl-2 and Bcl-X\u003csub\u003eL\u003c/sub\u003e expression were decreased and Bad and Bax expression were increased at the mitochondrial outer membrane in HEC50B cells. In conclusion, the dynamics of the Bcl-2 family during E2 -induced apoptosis was found to be different depending on the grade of endometrial cancer cells. We hope that the dynamics of Bcl-2 family proteins such as Bcl-X\u003csub\u003eL\u003c/sub\u003e and Bad will be used to diagnose the grade of malignant of endometrial cancer cells.\u003c/p\u003e","manuscriptTitle":"Mitochondrial Dynamics of Bcl-2 Family During E2-Induced Apoptosis Correlates with the Malignant of Endometrial Cancer Cells","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-01-31 19:52:32","doi":"10.21203/rs.3.rs-1260502/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"987c0b73-e224-4fc7-8168-945700420dff","owner":[],"postedDate":"January 31st, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2022-12-07T19:55:40+00:00","versionOfRecord":[],"versionCreatedAt":"2022-01-31 19:52:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1260502","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1260502","identity":"rs-1260502","version":["v1"]},"buildId":"_2-kVJe1T_tPrBINL-cwx","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}