Comparative analysis of RNA-sequencing in intraocular tumors

Abstract Background: Intraocular tumors are a class of serious diseases which may cause blindness and disability. This study aims to explore the underlying mechanisms of gene regulation in intraocular tumors. Methods: In this study, we firstly adopted RNA-sequencing technology to screen the differentially expressed genes in OCM-1 and Y79 cells compared with HRECs. Results: We found 8424 differentially expressed genes (4665 downregulated and 3759 upregulated) in OCM-1 cells compared with HRECs cells, and 7486 differentially expressed genes (4250 downregulated and 3236 upregulated) in Y79 cells compared with HRECs cells. In addition, the GO (Gene Ontology) terms was used to predict the potential functions of these differentially expressed genes and KEGG (Kyoto Encyclopedia of Genes and Genomes) was used to analyze the potential functions of these genes in the pathways. Furthermore, we identified the Top differentially expressed genes by using qRT-PCR assay in intraocular tumors. Conclusions: Here, we fully exposed the differentially expressed genes in intraocular tumor cell lines, which will improve understanding for gene regulation in intraocular tumors.