Exploration of inflammation-prognosis correlation and potential regulatory molecules in adamantinomatous craniopharyngioma

Purpose: To explore the inflammation-prognosis correlation and potential regulatory molecules in adamantinomatous craniopharyngioma (ACP) Methods 61 ACP patients who received surgery by either sub-resection or total-resection were included. Kaplan-Meier Curves were drafted to clarify patients’ prognosis in different sub-groups. Kinds of histological staining were used to evaluate the infiltration and activation of inflammatory cells. The expression dataset of GSE94349 was downloaded from Gene Expression Omnibus (GEO) database for series of bioinformatic analysis. Online bioinformatic analysis databases (Oncomine, GEPIA, TIMER, and NetworkAnalyst, et al) were used for a pan-cancer analysis on potential molecules. Finally, fluorescence in situ hybridization (FISH) and western bolt were conducted to verify the connection between target biomarkers and macrophages. Results The extent of resection was irrelevant to the progress free survival (PFS) of the ACP patients. Patients with high grade of inflammation showed worse prognosis than those with low and medium grade. More inflammatory cells infiltrated in recurrent tumor but represented with an immune inhibitory phenotype. Fraction of immune cells was calculated, and ACP was loaded with more M0 macrophages and plasma cells. SERPINs were identified and then a pan-cancer analysis was conducted to verify its value in clinic. At last, single cell sequencing database and FISH results reveals SERPINs mainly expressed in macrophages and monocytes. Conclusion Our study reveals a connection between inflammation response and poor prognosis in ACP patients. And SERPINs, especially SERPINE1 might be key inflammatory gene, which is relevant to macrophages.