Plant peptide hormone phytosulfokine promotes embryogenic development of calli in Pinus massoniana

Plant peptide hormone phytosulfokine promotes embryogenic development of calli in Pinus massoniana | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Plant peptide hormone phytosulfokine promotes embryogenic development of calli in Pinus massoniana Qunfeng Luo, Shan Hu, Zhaolei Deng, Zhenjun Gu, Qian Liu, Guang zhou, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4386542/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 11 Sep, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 5 You are reading this latest preprint version Abstract Pinus massoniana is a critical afforestation and ecological tree species in China. However, the continued existence of this pine is severely threatened by pine wilt disease. Somatic embryogenesis serves as a highly efficient clonal propagation approach. Although significant progress has been made in somatic embryogensis research on P.massoniana, resulting in the successful regeneration of plants, the limited embryogenic potential of improved cell lines and the loss of embryogenic properties resulting from prolonged proliferation have posed obstacles to the industrialization of SE production. Here, we investigated the effect of phytosulfokine on embryogenic development of cell lines from P. massoniana, and then leading to a cascade of physicochemical changes. Eight embryogenic cell lines of P. massoniana were used as research materials to observe phenotype and cytological changes. Physiological factors and the contents of nutrients and endogenous hormones were measured before and after phytosulfokine addition. We found that PSK promoted the embryogenic suspensor mass structure of embryogenic cell lines of P. massoniana. In addition, PSK accumulated soluble sugar, protein, and starch, and maintained redox homeostasis during the cell line proliferation process by reducing H2O2 levels. Further, endogenous hormone indole-3-acetic acid may be the main driver of the embryonic changes of cell lines. Our findings increase our understanding of how PSK affects somatic embryogensis in P. massoniana, thereby providing a valuable tool for establishing efficient somatic embryogensis systems in conifer species. Pinus massoniana somatic embryogenesis phytosulfokine endogenous hormone Full Text Supplementary Files SupplmentaryFile.docx Cite Share Download PDF Status: Published Journal Publication published 11 Sep, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Editorial decision: Major revisions 08 Jul, 2024 Reviewers agreed at journal 17 May, 2024 Reviewers invited by journal 12 May, 2024 Editor assigned by journal 11 May, 2024 First submitted to journal 08 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4386542","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":301494007,"identity":"e17ed546-ec23-40f9-94c1-2e936247f151","order_by":0,"name":"Qunfeng Luo","email":"","orcid":"","institution":"Guangxi Forestry Research Institute","correspondingAuthor":false,"prefix":"","firstName":"Qunfeng","middleName":"","lastName":"Luo","suffix":""},{"id":301494008,"identity":"3470dd30-4734-4638-8102-8b5cf39b0144","order_by":1,"name":"Shan 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