Brenneria Yuansilingia Sp. Nov., Isolated From Symptomatic Bark of Willow Canker 

Abstract Two Gram-negative, facultative anaerobic strains are isolated from the symptomatic tissue of willow bark canker. The cells grow at 4–41 ℃, pH 5.0–10.0, with optimal growth occuring at 30 °C and pH 7.0. Two novel strains share the highest 16S rRNA gene sequence similarity with Brenneria nigrifluens LMG 2694T (98.8%). In the phylogenetic trees based on sequences of 16S rRNA gene and four housekeeping genes (gyrB, rpoB, atpD and infB), two novel strains form a distinct branch from Brenneria species, indicating that two novel strains should belong to a novel species in the genus Brenneria, which is confirmed by the results of average nucleotide identity analysis and Digital DNA–DNA hybridization. Two novel strains have 81.9–85.0 % average nucleotide identity values with its closely relatives, B. nigrifluens LMG 2694T, B. alni DSM 11811T, B.corticis CFCC11842T, B. populi CFCC 11963T and B. corticis gBX10-1-2T, respectively, lower than the proposed species boundary cut-off for ANI (95-96 %). The main fatty acids are C16:0, C16:1 ω7c and C18:1 ω7c. The DNA G+C content is 53.5 -53.7 %. Based on these data, two novel strains represent a novel species within the genus Brenneria. The name Brenneria yuansilingia sp. nov. is proposed. The type strain is hezel4-2-4T (=CFCC 15597T = LMG 31719T).