Orai1 and CRAC channel dependence of VEGF-activated Ca2+ entry and endothelial tube formation.

Angiogenesis is an endothelial migration and tube formation process underlying new vessel growth from pre-existing blood vessels 1 . It has roles in wide-ranging physiological events and pathologies that include diabetic retinopathy, peripheral vascular disease, endometriosis, tissue regeneration, atherosclerosis, obesity, rheumatoid arthritis, and cancer 2 - 4 . Strategies to enhance or suppress angiogenesis (depending on the clinical situation) have attracted much attention. In the case of efforts to stimulate angiogenesis for cardiovascular therapy, preclinical data have been encouraging whereas late stage clinical trials have been disappointing 5 , 6 . Problems with the complexities of angiogenesis and the differing biology of old patients and young animal models have been suggested as explanations for the difficulties in translating to the clinic, suggesting that greater understanding and new intervention approaches are needed 6 . Strategies aimed at inhibiting angiogenesis to reduce tumour expansion have, however, seen success, although with limitations, for example, due to drug resistance, again because of the complexities of the angiogenesis 3 , 7 . Perhaps surprisingly, angiogenesis inhibition may have advantage in cardiovascular disease settings because it could be a means to suppress obesity and metabolic diseases that drive atherosclerosis 4 . Primary intercellular signaling molecules in angiogenesis are the vascular endothelial growth factors (VEGFs), which bind to VEGF receptors such as VEGFR2 1 , 7 . The receptors are tyrosine kinase receptors that link to down-stream signaling pathways such as monomeric G proteins and PI3 and MAP kinases but there is also evidence for associated intracellular Ca 2+ signals originating from phospholipase Cγ and inositol 1,4,5-trisphosphate 1 , 8 . These Ca 2+ events show the classical Ca 2+ -release followed by Ca 2+ -entry characteristics 8 , 9 . Jho et al 9 suggested that TRPC1 channels contribute to the Ca 2+ -entry mechanism and other investigators have suggested roles of TRPC6 channels in angiogenesis 10 , 11 . Synergism of VEGF and TRPC1 in zebrafish angiogenesis has been identified 12 . Nevertheless, the underlying Ca 2+ channel mechanisms and their significance remain incompletely understood. A Ca 2+ channel type with emerging prominence is the Ca 2+ -release activated Ca 2+ (CRAC) channel 13 - 15 . It is an extremely low conductance, highly calcium-selective channel that opens in response to any signal that depletes Ca 2+ in intracellular stores 14 . A pore-forming subunit of the channels is Orai1, a member of the Orai family of tetraspanin-related membrane proteins 13 - 15 . The channel type was discovered in immune cells and individuals with disrupted Orai1 function, because of a R91W mutation, have severe combined immune deficiency 13 , 16 . With the identification of Orai1, however, it has become increasingly apparent that the CRAC channel is widely distributed and thus not restricted to the immune system. Its general activation mechanism through STIM1 of depleted Ca 2+ stores 14 is common to many cell types, such that most mammalian cells exhibit store-operated Ca 2+ -entry that may be accounted for, at least partly, by Orai1. The general activation signal of store-depletion suggests that it is a nodal point in the signaling of multiple agonists, presenting an opportunity for overcoming problems of redundancy, compensation and drug resistance. Importantly, there is specific evidence that human umbilical vein endothelial cells (HUVECs) express Orai1, exhibit store-operated Ca 2+ -entry that depends on Orai1, and contain a small CRAC channel-like current 17 . HUVECs are commonly used as a basis for in vitro angiogenesis assays. The relevance of CRAC channels and Orai1 to the VEGF responses of these, or other, endothelial cells is unknown. Here we investigated the relevance of Orai1 and CRAC channel-related Ca 2+ -entry to VEGF-evoked Ca 2+ -entry and endothelial cell behavior, using a variety of established angiogenesis assays 1 . In addition to molecular manipulation of Orai1, which may not only relate to the CRAC channel, we investigated a chemical blocker that has been found to have selectivity for CRAC channels in immune cells 18 , 19 . HUVECs, endothelial cells cultured from human saphenous vein obtained at coronary artery bypass operations, and late outgrowth endothelial progenitor cells (EPCs) from healthy volunteers were used in addition to chicken embryo chorioallantoic membrane for in vivo angiogenesis. EPCs may be important in vasculogenesis, vascular repair, and tumour vessel formation 3 , 20 , 21 .

HUVECs were cultured in EGM-2 growth medium supplemented with EGM-2 bullet kit (Lonza) at 37 °C in a humidified atmosphere containing 5 % CO 2 . Provided in the Supplementary Information are the methods for culture of EPCs, endothelial cells from human saphenous vein, HEK 293 cells with and without stable expression of TRPC6, and CHO cells stably expressing TRPV4. Cells at 90 % confluence were transfected with 20 nmoles/L siRNA using Lipofectamine 2000 in OptiMEM as per the manufacturer’s instructions (Invitrogen). Sequences of siRNA probes are given in the Supplementary Information . Fresh EGM-2 growth medium was added after 4-6 h and the cells were analyzed 48 h after transfection. To validate effectiveness of siRNA probes, mRNA was isolated and quantified by real-time RT-PCR (see Supplementary Information ). Orai1 siRNA 1 (Or1.si.1) effectiveness was also confirmed by western blotting (see Supplementary Information ). When cells were transfected with human Orai1 (accession number BC013386 ) or its dominant negative (DN) mutant (R91W) in pcDNA6, 0.2 μg DNA was added. Orai1 cDNA was intended to saturate Or1.si.1 in rescue experiments. eYFP-STIM1 was in pDS (from J. Liou, Stanford, Calif.). Endothelial cells were incubated with fura-2AM for 1 h at 37 °C followed by a 0.5 h wash at room temperature (21± 2 °C). Fluo-4AM was used in place of fura-2AM for recordings of over-expressed TRPC6 and TRPV4 activity. Measurements were made at room temperature on a 96-well plate reader (FlexStation, Molecular Devices). The change () in intracellular calcium (Ca 2+ ) concentration was indicated as the ratio of fura-2 emission intensities for 340 nm and 380 nm excitation (F ratio). Wells within columns of the 96-well plate were loaded alternately for test and control conditions. The recording solution contained (mmoles/L): 130 NaCl, 5 KCl, 8 D-glucose, 10 Hepes, 1.2 MgCl 2 , 1.5 CaCl 2 , titrated to pH 7.4 with NaOH. When Ca 2+ -free extracellular solution was used, CaCl 2 was omitted; for Ca 2+ addback, Ca 2+ was 0.2 mmoles/L. S66 was added 15 min before Ca 2+ measurements at room temperature. Cells were labeled with antibodies using standard immunofluorescence protocols (details are given in the Supplementary Information ) and visualized using a deconvolution system (Applied Precision Instruments, Seattle, WA) based on the Olympus IX-70 inverted microscope with x60 objective (NA 1.4). Methods are given in the Supplementary Information . Cell migration assays were performed using a modified Boyden chamber (8-μm pores; BD Biosciences, UK). HUVECs transfected with siRNA or pre-treated for 24 h with S66 were serum starved in EGM-2 for 4 h, resuspended at 5 × 10 4 cells/mL in EGM-2 containing 0.4 % foetal calf serum (FCS), and loaded in the upper chamber. The lower chamber contained 0.4 % FCS supplemented with the chemo-attractant 20 ng/mLVEGF (Sigma). After incubation for 4 h at 37 °C in a 5 % CO 2 incubator, the inserts were fixed with 70 % ethanol for 2 min. Cells on duplicate membranes were scraped from the upper surface, and migrating cells underneath were stained with hematoxylin and eosin and evaluated by counting cells in 6 randomly chosen fields under light microscopy. Methods for measuring cell viability are given in the Supplementary Information . HUVECs were detached by trypsinization and, after neutralization of trypsin, were counted and resuspended in EGM-2 containing 1 % FCS and 2 ng/mL VEGF at 4 × 10 5 cells/mL. HUVECs were seeded at 2 × 10 4 cells per well in Matrigel pre-coated BD Biosciences 96-well plates (Catalog #354149). Tube formation was quantified 18-20 h later. Cells were treated with chemical agents for 24 h or transfected 48 h before seeding on the 96-well plate. Chemical agents were maintained throughout experiments. EPCs were plated on thin-layer Matrigel pre-coated 24-well plates (BD Biosciences, Catalog #354605) at 5 × 10 4 cells/well in 1% FCS EGM-2 with 2 ng/mL VEGF and tube formation was quantified 20 h later. Digital images of endothelial tubes were obtained by bright-field light microscopy. Tube formations were measured blind by an independent observer, giving: (i) total tube length per image; and (ii) number of complete loops (circles) per image. Chicken embryo chorioallantoic membrane angiogenesis assays were performed according to a published protocol 22 . Briefly, fertilized chicken eggs were cleaned with 70 % ethanol and incubated at 37 °C under 60 % humidity in an egg incubator. On day 3, albumen (2–3 mL) was aspirated at the acute pole using a sterile 25-G hypodermic needle in order to create an air sac directly over the chorioallantoic membrane. A square window (10 ×10 mm) was then cut in the shell and was sealed with tape. Eggs were returned to the incubator and, on day 8, the window was opened under sterile conditions and a sterilized gelatin sponge pre-infused with VEGF (100 ng/mL) and S66 (20 μmoles/L) was placed on the chorioallantoic membrane. On day 11, the vessels surrounding each sponge were counted. S66 (3-fluoropyridine-4-carboxylic acid (2′,5′-dimethoxybiphenyl-4-yl)amide) was a gift from GSK (GSK1349571A); its chemical identity was confirmed independently by LC-MS analysis (Leeds) and chemical synthesis. S66 is an abbreviation of “Synta 66” from patent WO 2005/009954 18 , 19 . All other reagents were from Sigma unless specified in the Supplementary Information . Averaged data are presented as mean±s.e.mean. Data were produced in pairs (test and control) and compared using t tests with statistical significance indicated by * ( P 0.05). The number of independent experiments is indicated by n . For multi-well assays, the number of replicate wells is indicated by N.

CRAC channel function was first detected as a component of store-operated Ca 2+ -entry, which was investigated using an intracellular Ca 2+ indicator to detect Ca 2+ -entry when extracellular Ca 2+ was added back to endothelial cells that had been store-depleted using the pharmacological agent thapsigargin (e.g. Fig 1a ). The Ca 2+ add-back signal was suppressed when Orai1 expression was knocked-down by short interfering (si) RNA, as shown by the example data for EPCs ( Fig 1a , Supplementary Fig I and Fig II ) and the mean data in Fig 1c . An alternative approach for disruption of Orai1 is expression of exogenous R91W mutant of Orai1, which acts as a dominant negative (DN) to inhibit endogenous Orai1 and CRAC channel function 13 , 23 , 24 . DN-Orai1 also inhibited the Ca 2+ -entry, as shown by comparative analysis in HUVECs ( Fig 1b, c ). The data confirm previous siRNA studies of HUVECs 17 and extend the findings to DN-Orai1 and EPCs. It was hypothesized that the above Ca 2+ signals were relevant to the action of VEGF because VEGF caused Ca 2+ release from intracellular stores, as shown for example in HUVECs ( Supplementary Fig III ). In the continuous presence of extracellular Ca 2+ , VEGF evoked a transient followed by a sustained elevation of the intracellular Ca 2+ concentration ( Fig 1d ), suggesting that there was Ca 2+ entry after transient Ca 2+ release. Orai1 siRNA reduced both the transient and sustained effects of VEGF by about 42 % ( Fig 1d, e ). Whole-cell patch-clamp recordings revealed that VEGF-evoked ionic current was below the detectable amplitude when using CRAC channel recording conditions in the presence of extracellular Ca 2+ ( Supplementary Figs IV-VIII ), consistent with prior information on endothelial cells 17 . The data suggest that Orai1 contributes substantially to VEGF-evoked Ca 2+ signals that are mediated by sub-pA ionic currents. A previous report suggested that TRPC1 also confers VEGF-evoked Ca 2+ entry 9 . We similarly observed suppression by anti-TRPC1 blocking antibody ( Fig 1f ) but the effect was relatively small and variable, not achieving statistical significance across all experiments ( Fig 1g ). Previous research suggested that VEGFR2 is the mediator of VEGF-evoked Ca 2+ signals 9 . Consistent with this mechanism the multikinase inhibitors sorafenib and vatalanib potently suppressed VEGF-evoked Ca 2+ rises ( Fig 2a , Supplementary Fig IX ). Phospholipase Cγ has also been implicated 9 and so a phospholipase C inhibitor U73122 was investigated. U73122 similarly inhibited VEGF-evoked Ca 2+ rises ( Fig 2a , Supplementary Fig IX ). Inositol-1,4,5-trisphosphate generated by phospholipase C was, therefore, presumed to be the messenger evoking Ca 2+ -release. Studies of other cell types have suggested that the ensuing depletion of Ca 2+ stores causes clustering of STIM1 in endoplasmic reticular membranes and that this STIM1 binds to and activates Orai1 channels in the plasma membrane 14 . To investigate this mechanism, HUVECs were transfected with eYFP-tagged STIM1 which showed the expected 14 microtubule-like localization pattern ( Fig 2b ). VEGF caused marked clustering of eYFP-STIM1 ( Fig 2c ). Additional experiments were carried out where endogenous STIM1 was knocked-down by RNA interference ( Fig 2d , Supplementary Fig I ). STIM1 siRNA suppressed VEGF-evoked Ca 2+ signals ( Fig 2d ). The data suggest that the pathway for VEGF to evoke Orai1-dependent Ca 2+ entry is: VEGFR2; phospholipase Cγ; inositol-1,4,5-trisphosphate; inositol-1,4,5-trisphosphate receptor; Ca 2+ release from intracellular stores; STIM1 activation and clustering; STIM1 binding to Orai1; Orai1 (CRAC) channel activation (summarized in Supplementary Fig X ). Using specific mouse monoclonal anti-VEGFR2 and rabbit polyclonal anti-Orai1 antibodies ( Supplementary Figs II, XI ) we investigated the subcellular localizations of endogenous VEGFR2 and Orai1 in HUVECs ( Fig 2e ). Partial colocalization was observed in the vicinity of the plasma membrane, most obviously when observing in a focal plane above the adherent surface, where the nucleus was located ( Fig 2e , Supplementary Fig XI-XIII ). The image in Fig 2e was captured from a cell in the presence of VEGF but VEGF did not obviously change the degree of colocalization ( Supplementary Fig XII ). The data suggest that these proteins share a subcellular compartment, possibly to enhance signaling efficiency. A key process regulated by VEGF is endothelial cell migration 1 which can be quantified in modified Boyden chambers in which the cells migrate through 8 μm pores towards VEGF 1 ( Fig 3a , Supplementary Fig XIV ). It was observed that Orai1 siRNA suppressed HUVEC migration, as shown by the example ( Fig 3a ) and mean data ( Fig 3b ). A similar but weaker effect of DN-Orai1 was observed ( Fig 3b ). The data suggest that Orai1 has a positive role in endothelial cell migration and may therefore play a role in angiogenesis. To investigate the relevance to angiogenesis we first quantified HUVEC tube and loop formation on Matrigel as an in vitro assay of angiogenic potential 1 , 25 . Examples of the structures formed by the cells are shown in Fig 3c . Disruption of Orai1 by DN-Orai1 inhibited the tube formation ( Fig 3c ). Therefore, extensive experiments were performed to test the hypothesis that Orai1 has a role in this phenomenon ( Fig 3d ). Experiments were carried out in test and control pairs where the white bars are mean control data and the black bars to the right are mean test data ( Fig 3d ). Two different Orai1 siRNAs (Or1.si.1 and Or1.si.2) independently inhibited tube length and loop formation, whereas an inactive siRNA (Or1.neg.) had no effect ( Fig 3d ). In cells subjected to Orai1 siRNA, normal tube length and formation could be rescued by expression of exogenous wild-type (WT) Orai1 clone ( Fig 3d ). EPCs were studied less extensively but loop structures formed by the cells were found to be largely prevented by Orai1 siRNA ( Fig 3e, f ). The data suggest that Orai1 makes a significant contribution to angiogenic behaviour of endothelial cells. The above experiments suggest a role of Orai1 but the results may not be explained by a role of CRAC channels because Orai1 could have multiple functions. Therefore, we sought a specific chemical blocker of CRAC channels as a further test of the role of the channels. The patent WO 2005/009954 describes the compound 3-fluoropyridine-4-carboxylic acid (2′,5′-dimethoxybiphenyl-4-yl)amide as an inhibitor of CRAC channels in immune cells. We refer to the compound as S66. It has previously been reported to specifically inhibit CRAC channel-related signals in immune cells with an IC 50 of 1.4-3.0 μmoles/L 18 , 19 . The Ca 2+ add-back signal in store-depleted HUVECs was strongly suppressed by S66 ( Fig 4a ). The effect occurred with high potency, showing an IC 50 of 25.5 nmoles/L ( Fig 4b ). Direct comparisons with immune cell Ca 2+ entry under the same conditions confirmed that there was higher potency against HUVECs and EPCs ( Supplementary Fig XV ). Specificity of S66 was indicated by investigation of two other types of Ca 2+ channel that are expressed and functional in endothelial cells, TRPC6 10 , 11 and TRPV4 26 , 27 . Over-expressed TRPC6 was activated by the lipid 1-oleoyl-2-acetyl- sn -glycerol ( Fig 4c ) whereas over-expressed TRPV4 was activated by 4α-phorbol-didecanoate ( Fig 4d ). S66 (5 μmoles/L) had no significant effect on these channels ( Fig 4c, d ). The data suggest that S66 is an inhibitor of endothelial store-operated Ca 2+ -entry and lacks effect on TRPC6 and TRPV4. S66 was tested against VEGF-evoked Ca 2+ signals in the presence of extracellular Ca 2+ ( Fig 4e, f ). It caused strong inhibition of sustained Ca 2+ -entry while lacking effect on the initial transient response ( Fig 4e, f ). These data contrast with the Orai1 siRNA data of Fig 1 (d, e) because there was specificity for the sustained Ca 2+ response, suggesting that S66 did not affect VEGF receptors or Ca 2+ -release. S66 also did not affect STIM1 clustering ( Supplementary Fig XVI ). To investigate the relevance of the action of S66 to other types of endothelial cell, VEGF responses were investigated in EPCs and cultures of saphenous vein endothelial cells (SVECs) from patients undergoing coronary artery bypass graft surgery. As observed in HUVECs, S66 was a strong inhibitor of the sustained VEGF response but not the transient response ( Fig 4f ). The residual S66-resistant Ca 2+ entry in HUVECs might be accounted for by TRPC1 ( Fig 1f, g ). In saphenous vein endothelial cells the S66-resistant component was larger ( Fig 4f ), as was the TRPC1 contribution ( Supplementary Fig XVII ). The data are consistent with CRAC channels mediating a substantial fraction of the sustained Ca 2+ -entry evoked by VEGF and with the amplitude of this contribution varying across endothelial cell types. S66 was also investigated against endothelial cell migration. The cell migration was inhibited by about 50 % with maximum effect at 2 μmoles/L S66 ( Fig 5a ), suggesting that the S66-sensitive mechanism had a positive modulator role. S66 lacked effect on endothelial cell viability ( Fig 5b ). To investigate the impact on angiogenesis we performed in vitro and in vivo assays. In in vitro assays S66 restricted tube length and reduced the number of loops, conferring 60-70 % inhibition ( Fig 5c, d ). For in vivo analysis we used the established angiogenesis assay of the chick chorioallantoic membrane, where new blood vessels form around VEGF-infused sponges 1 , 22 . S66 had striking inhibitory effects on the growth of these vessels ( Fig 5e, f ). The data suggest that S66 was an inhibitor of VEGF-induced endothelial cell migration and angiogenesis without effect on cell viability.

The study suggests that a CRAC channel protein (Orai1) is important for store-operated and VEGF-evoked Ca 2+ entry in endothelial cells, with Orai1 disruption leading to reduced endothelial cell migration and tube formation (see the summary diagram of Supplementary Fig X ). Because Orai1 may have CRAC channel independent roles we sought to further investigate the hypothesis that CRAC channels are involved by seeking a specific chemical blocker of the channels. S66 was found to be a strong, potent and specific inhibitor of store-operated Ca 2+ -entry. Importantly, it replicated the effects of Orai1 disruption on sustained endothelial cell Ca 2+ -entry and migration and tube formation, and inhibited VEGF-evoked angiogenesis in vivo . Suppression of the transient VEGF Ca 2+ -response by Orai1 siRNA but not S66 may be explained by partial store-depletion resulting from the long-term disruption of CRAC channels or a CRAC channel-independent role of Orai1. CRAC channels and Orai1 have mostly been associated with immune cell function 13 , 28 . Therefore, inhibitors of CRAC channels and Orai1 are predicted to be immune-suppressive. Our S66 data suggest that it may be possible to minimize such effects because of the higher potency in endothelial cells. Nevertheless, inhibitor effects on immune cell function could be advantageous in conditions such as obesity or cancer where, for example, low-grade long-term inflammation and late-stage aggressive immune responses have deleterious effects 29 , 30 . Although the effect of S66 seems to open new therapeutic potential it is a limitation that we do not yet know its mechanism of action. Our data suggest that it does not affect Ca 2+ -release ( Fig 4e ) or STIM1 clustering ( Supplementary Fig XVI ), putting its site of action downstream and thus perhaps at Orai1 or another CRAC channel component. Disease in Orai1-deficient patients is severe but apparently limited to immunodeficiency, congenital myopathy and ectodermal dysplasia 16 , 31 . These patients have intact vasculatures and so Orai1 is not obligatory for vasculogenesis in humans. Orai1-deficient mice are either runted and display immune deficiency or die just before or after birth for reasons that are not yet clear 32 , 33 . Such observations from patients and mutant mice are consistent with our data showing suppression but not abolition of endothelial migration and tube formation following Orai1 disruption. That is, we hypothesize that Orai1’s role in endothelial function is as a positive modulator rather than obligatory factor. It is, therefore, conceivable that there are compensatory mechanisms for Orai1-deficiency, perhaps involving other Orai proteins or other types of Ca 2+ channel protein that have roles in VEGF responses and angiogenesis. Indeed, our data are consistent with a contribution of TRPC1 and there is good evidence for roles of TRPC6 10 , 11 which may be particularly relevant to slowly-developing VEGF effects outside the time-frame of our Ca 2+ i measurements (see Supplementary Fig X ). Integration of Orai1 and TRPC1 is indicated to occur, for example, through STIM1 34 ( Supplementary Fig X ). In summary, positive roles of Orai1 in endothelial cell VEGF Ca 2+ i -responses, migration and tube formation are indicated. The effects appear to result substantially from Orai1’s role in CRAC channels because of the additional finding that a specific CRAC channel blocker had mostly similar effects to Orai1 disruption. Unexpected high potency of the blocker at endothelial compared with immune cells suggested, nevertheless, a distinct characteristic of the endothelial CRAC channel and the opportunity for endothelial specificity. In addition to the mechanistic insight provided by this study there is the suggestion that the process could be an attractive target for therapeutic modulation of angiogenesis in inflammatory disease situations that include cancer and metabolic syndrome associated with obesity. What Is Known? Orai1 is a membrane protein component of a T cell Ca 2+ entry mechanism that is defective in severe combined immune deficiency Depletion of intracellular Ca 2+ stores is an activation signal for CRAC channels in T cells Orai1 is expressed in human umbilical vein endothelial cells, which contain CRAC channel-like signals Orai1 is a membrane protein component of a T cell Ca 2+ entry mechanism that is defective in severe combined immune deficiency Depletion of intracellular Ca 2+ stores is an activation signal for CRAC channels in T cells Orai1 is expressed in human umbilical vein endothelial cells, which contain CRAC channel-like signals What New Information Does This Article Contribute? Endothelial Orai1 stimulates Ca 2+ entry, cell migration and tube formation evoked by vascular endothelial growth factor (VEGF) A specific chemical blocker of CRAC channels inhibits VEGF-evoked Ca 2+ -entry, endothelial cell migration and endothelial tube formation in vitro , and angiogenesis in vivo Endothelial Ca 2+ -entry is more sensitive to the CRAC channel blocker than immune cell Ca 2+ -entry Endothelial Orai1 stimulates Ca 2+ entry, cell migration and tube formation evoked by vascular endothelial growth factor (VEGF) A specific chemical blocker of CRAC channels inhibits VEGF-evoked Ca 2+ -entry, endothelial cell migration and endothelial tube formation in vitro , and angiogenesis in vivo Endothelial Ca 2+ -entry is more sensitive to the CRAC channel blocker than immune cell Ca 2+ -entry This study was conducted because of paucity of information about the significance of Orai1 and CRAC channels in endothelial cell biology and to determine if a pharmacological approach exists that could enhance mechanistic insight and facilitate clinical exploitation in the cardiovascular system. It was confirmed that disruption of Orai1 suppressed Ca 2+ -entry evoked by pharmacological store-depletion in human umbilical vein endothelial cells and the finding was extended to endothelial progenitor cells derived from blood of healthy volunteers, which are thought to be involved in endothelial repair and pathological angiogenesis. Furthermore, Ca 2+ -entry evoked by the physiological factor, VEGF, was also shown to depend on Orai1. Because VEGF has pivotal roles in evoking endothelial cell migration and angiogenesis we investigated if Orai1 is relevant to these key endothelial cell properties. By using an established in vitro angiogenesis assay we were able to carry out a thorough molecular investigation of the role of Orai1, including rescue of function by exogenous Orai1 clone after knock-down of native expression. The data show conclusively that Orai1 is important in tube formation. Reduced capacity for cell migration was implicated as a contributing factor because this parameter also depended on Orai1 and is central to angiogenesis. Because Orai1 may have functions in addition to CRAC channel formation we sought a specific CRAC channel blocking substance for further studies. We found that a substance with previously described specificity but modest potency at immune cell CRAC channels has 100-fold higher potency at endothelial Ca 2+ entry. The substance blocked VEGF-evoked Ca 2+ -entry but not Ca 2+ -release, and endothelial cell migration and tube formation were suppressed without effect on cell viability. Importantly, the substance also inhibited VEGF-evoked angiogenesis in vivo . The study has shown us that Orai1 and CRAC channels are relevant to major aspects of endothelial biology: VEGF signaling, endothelial cell migration, and angiogenesis. Identification of a specific and potent chemical modulator increases the likelihood that there will be opportunity for therapeutic benefit.