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by gemini-2.5-flash-lite, 2026-07-17
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This study developed a subcutaneous endometriosis rat model by implanting uterine fragments and demonstrated significant endometrioma weight changes in response to estradiol, medroxyprogesterone acetate, triptorelin pamoate, and acetylsalicylic acid treatments.
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by claude@2026-07, 2026-07-17
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The paper aimed to develop and characterize a reproducible rat model of subcutaneous endometriosis to study pathophysiology and drug effects. Fifty 3-month-old female Wistar rats were divided into control and four treatment groups (estradiol at 2.5, 5, or 10 mg/kg subcutaneously; medroxyprogesterone acetate at 0.5, 2, or 5 mg/kg subcutaneously; triptorelin pamoate at 0.18 or 0.56 mg/kg subcutaneously; or acetylsalicylic acid at 3 mg/kg orally) and all animals received autoimplanted 4×4 mm uterine tissue fragments subcutaneously; endometrioma size was measured on days 1, 7, 14, and 21 with relative wet/dry weights, and endometrial-like tissue was confirmed histologically. The greatest endometrioma weight gain occurred on day 14, and drug-associated changes included significant differences with high-dose estradiol, significant (but not dose-dependent) reductions with medroxyprogesterone acetate, and significant weight reductions with triptorelin pamoate and acetylsalicylic acid. The study’s main limitation as stated in the abstract is that it uses a single implantation setting and endpoint-focused measurements rather than broader mechanistic readouts. This paper is centrally about endometriosis — it develops a subcutaneous rat endometriosis model for testing drug effects and studying pathophysiology.
Abstract
PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.
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http://repositorio.ufc.br/handle/riufc/18140
| Type: | Artigo de Periódico |
| Title: | Development of a subcutaneous endometriosis rat model |
| Authors: | Pereira, Francisco Edson Ximenes Gomes Almeida, Paulo Roberto Carvalho de Dias, Bruno Hállan Meneses Vasconcelos, Paulo Roberto Leitão de Guimarães, Sérgio Botelho Guimarães Medeiros, Francisco das Chagas |
| Keywords: | Endometriose;Pamoato de Triptorrelina |
| Issue Date: | Jan-2015 |
| Publisher: | Acta Cirúrgica Brasileira |
| Citation: | PEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015. |
| Abstract: | PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs. |
| URI: | http://www.repositorio.ufc.br/handle/riufc/18140 |
| ISSN: | On-line version 1678-2674 |
| Appears in Collections: | DCIR - Artigos publicados em revista científica |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2015_art_fexgpereira.pdf | 785,53 kB | Adobe PDF | View/Open |
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