Characterization of Wheat Yellow Rust Resistance in Bread Genotypes at Adult and Seedling Plant Resistance in South Ethiopia

Characterization of Wheat Yellow Rust Resistance in Bread Genotypes at Adult and Seedling Plant Resistance in South Ethiopia | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Characterization of Wheat Yellow Rust Resistance in Bread Genotypes at Adult and Seedling Plant Resistance in South Ethiopia Kabna Asefa, Girma Adugna, Ayele Badebo This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8455355/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Yellow rust is the most serious wheat disease in Ethiopia, particularly in highland areas. Field and greenhouse experiments were conducted to characterize the resistance level of bread wheat genotypes to virulent races at seedling and adult plant resistance stages in Ethiopia. A total of 111 advanced Ethiopian bread wheat breeding lines and varieties were characterized under greenhouse and field conditions (two hotspot areas). Seedling resistance characteristics were assessed under greenhouse conditions using three virulent races (PstS2 + v1 + v27, PstS11, and Other/NEW) through artificial inoculations at Kulumsa, and the same entries were used to evaluate resistance under field conditions. In both field experiments, final rust severity, area under disease progress curves, disease progress rate, coefficient of infection, and head infection showed highly significant differences among genotypes. To further understand the relationships among genotypes and key resistance traits, Principal Component Analysis (PCA), cluster analysis, resistance mapping, and correlation studies were performed. PCA identified traits contributing most to genotype differentiation. Cluster analysis grouped the genotypes into four distinct clusters based on their resistance responses. Of the tested genotypes, 58.8% and 10.8% exhibited characterized resistance at seedling and adult plant growth stages, respectively, whereas 4.5% showed resistance at both the greenhouse and two hotspot field areas. Bread wheat genotypes that are resistant at the seedling and adult plant stages to the prevailing races have also been identified. These useful wheat genotypes could be promoted to breeders and farmers after their evaluation for the most important variables, such as yield and reaction to other diseases. yellow rust resistance resistance genotypes Full Text Additional Declarations No competing interests reported. Supplementary Files sumplementdata.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8455355","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":566666623,"identity":"ee0ff08a-63f2-4bd3-b779-cc4910f1b4da","order_by":0,"name":"Kabna Asefa","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA6klEQVRIiWNgGAWjYBACCQYeCIO9+QDjAyDNw0e0Fp5jCcwGIJqNFC1sEiAGQS2S7WePPfhRUyfPw8b8rPJrjp0MGwPzw0c38GiR5slLN+w5dtiwh43N7LbstmSgw9iMjXPwaJFjyDGT4GE7wLhfvsHstuQ2ZqAWHjZpvFr435hJ/vlXZ9/Dxv6tWHJbPWEt0hI5ZtK8bcyJPWw8Zowftx0mrEVyxrs0adm+w8lALcXSjNuOA4OBgF8kzucek3zzrc4W6LCNH39uq7bnZ29++BifFhTADI4jZmKVgwDjD1JUj4JRMApGwYgBAPldQAT9Q/BzAAAAAElFTkSuQmCC","orcid":"","institution":"Bako Agricultural Research Center, Oromia Agricultural Research Institute (OARI)","correspondingAuthor":true,"prefix":"","firstName":"Kabna","middleName":"","lastName":"Asefa","suffix":""},{"id":566666624,"identity":"fd265e8a-8a16-47b7-ab81-ad0c5374eecb","order_by":1,"name":"Girma Adugna","email":"","orcid":"","institution":"College of Agriculture and Veterinary Medicine, Jimma University, P.O. 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Field and greenhouse experiments were conducted to characterize the resistance level of bread wheat genotypes to virulent races at seedling and adult plant resistance stages in Ethiopia. A total of 111 advanced Ethiopian bread wheat breeding lines and varieties were characterized under greenhouse and field conditions (two hotspot areas). Seedling resistance characteristics were assessed under greenhouse conditions using three virulent races (PstS2\u0026thinsp;+\u0026thinsp;v1\u0026thinsp;+\u0026thinsp;v27, PstS11, and Other/NEW) through artificial inoculations at Kulumsa, and the same entries were used to evaluate resistance under field conditions. In both field experiments, final rust severity, area under disease progress curves, disease progress rate, coefficient of infection, and head infection showed highly significant differences among genotypes. 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These useful wheat genotypes could be promoted to breeders and farmers after their evaluation for the most important variables, such as yield and reaction to other diseases.\u003c/p\u003e","manuscriptTitle":"Characterization of Wheat Yellow Rust Resistance in Bread Genotypes at Adult and Seedling Plant Resistance in South Ethiopia","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-01-06 11:14:32","doi":"10.21203/rs.3.rs-8455355/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"74ce11c5-075b-46ef-b440-7f1228dcaefa","owner":[],"postedDate":"January 6th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-01-06T11:14:32+00:00","versionOfRecord":[],"versionCreatedAt":"2026-01-06 11:14:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8455355","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8455355","identity":"rs-8455355","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}