Real-time Monitoring by Interferometric Light Microscopy of Phage Suspensions for Personalised Phage Therapy

Real-time Monitoring by Interferometric Light Microscopy of Phage Suspensions for Personalised Phage Therapy | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Real-time Monitoring by Interferometric Light Microscopy of Phage Suspensions for Personalised Phage Therapy Benjamine Lapras, Camille Merienne, Emma Eynaud, Lea Usseglio, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4459652/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 30 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Phage therapy uses viruses (phages) against antibiotic resistance. Tailoring treatments to specific patient strains requires stocks of various highly concentrated purified phages. It therefore faces challenges: titration duration and specificity to a phage/bacteria couple; purification affecting stability; and highly concentrated suspensions tending to aggregate. To address these challenges, interferometric light microscopy (ILM), characterising particles (size, concentration, and visual homogeneity) within minutes, was applied herein to myovirus phage suspensions. Particle concentration was linearly correlated with phage titre (R²>0.97, slope: 3 particles/plaque forming units (PFU)) at various degrees of purification, allowing to estimate phage titre for suspensions ≥3.108 PFU/mL, thereby encompassing most therapeutic doses. Purification narrowed and homogenised particle distribution while maintaining therapeutic concentrations. When compared to dynamic light scattering, electrophoretic mobility, and UV/Visible-spectroscopy, ILM best detected aggregates according to our homemade scoring. The present proof-of-concept positions ILM as a valuable quality control tool for phage suspensions, paving the way for further investigations. Biological sciences/Microbiology/Antimicrobials Physical sciences/Nanoscience and technology/Techniques and instrumentation/Characterization and analytical techniques bacteriophages therapeutic phage suspension virus quantification fast purification monitoring viral stability aggregation Full Text Additional Declarations Competing interest reported. Myriade, the manufacturer of Videodrop® loaned the equipment for one month. However, Myriade had not provided funding for the study and had no role in the design of the study, in the collection, analyses, or interpretation of data, in the decision and the way to publish the results. Therefore, none author is having conflict of interest. Supplementary Files 20240522sci.repsupplementary.docx Cite Share Download PDF Status: Published Journal Publication published 30 Dec, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 09 Sep, 2024 Reviews received at journal 08 Sep, 2024 Reviewers agreed at journal 03 Sep, 2024 Reviews received at journal 13 Aug, 2024 Reviewers agreed at journal 04 Aug, 2024 Reviewers invited by journal 16 Jun, 2024 Editor assigned by journal 30 May, 2024 Editor invited by journal 30 May, 2024 Submission checks completed at journal 27 May, 2024 First submitted to journal 22 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4459652","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":310649922,"identity":"f91bfdb8-fa8b-42b7-b9a4-9eaf10e34234","order_by":0,"name":"Benjamine 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