Human PARPs modify RNA nucleobases in vitro and in cells

SUMMARY ADP-ribosylation is known as a protein modification, yet recent studies have expanded the range of ADP-ribosyltransferase (ART) substrates to include nucleic acids. tRNA 2′-phosphotransferase 1 (TRPT1) and several PARP family members can modify the 5′-phosphate of single-stranded RNA. Here, we show that PARP10 and PARP15 extend this activity beyond the 5′-phosphate terminus and generate N3-ADP-ribosyl uracil and N1-ADP-ribosyl guanine bases. The base-linked ADP-ribosylation is reversed selectively by the macrodomain-containing hydrolase TARG1. In TARG1 knockout cells, N1-ADP-ribosyl guanine can be detected. Together, these findings establish guanine and uracil ADP-ribose as two novel nucleotide modifications and reveal PARP15 and TARG1 as an enzyme pair which can dynamically regulate guanine ADP-ribosylation in living cells. Competing Interest Statement The authors have declared no competing interest. Footnotes ↵5 Lead contact