Laminar organization of pyramidal neuron cell types defines distinct CA1 hippocampal subregions

Laminar organization of pyramidal neuron cell types defines distinct CA1 hippocampal subregions | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Laminar organization of pyramidal neuron cell types defines distinct CA1 hippocampal subregions Michael Bienkowski, Maricarmen Pachicano, Shrey Mehta, Angela Hurtdado, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6581037/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 03 Dec, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Abstract Investigating the cell type organization of hippocampal CA1 is essential for understanding its role in memory and cognition and its susceptibility to neurological disorders like Alzheimer’s disease and epilepsy. Multiple studies have identified different organizational principles for gene expression and how it reflects cell types within the CA1 pyramidal layer including gradients or mosaic. Here, we identified sublaminar gene expression patterns within the CA1 pyramidal layer that extends across the entire hippocampal axis. Our findings reveal that CA1 subregions (CA1d, CA1i, CA1v, CA1vv) contain differentially distributed layers of constituent cell types and can be identified by regional gene expression signatures. This work offers a new perspective on the organization of CA1 cell types that can be used to further explore hippocampal cell types across species. Biological sciences/Neuroscience/Cellular neuroscience Biological sciences/Neuroscience/Molecular neuroscience Full Text Additional Declarations There is NO Competing Interest. Supplementary Files MPachicanoSupplementaryInformation.pdf Supplemental Information SupplementaryTable1.csv Supplementary Table 1 SupplementaryTable2.csv Supplementary Table 2 SupplementaryTable3.csv Supplementary Table 3 SupplementaryTable4.csv Supplementary Table 4 SupplementaryVideo1.mp4 Supplementary Video 1 Cite Share Download PDF Status: Published Journal Publication published 03 Dec, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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