siRNA knockdown validation 101: Incorporating negative controls in antibody research
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OA: closed
Abstract
More antibody validation protocols would identify non-specific reagents – a major source of irreproducible research – with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00